385 results about "Bacterial pollution" patented technology

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

The invention discloses a novel automatic cleaning machine for the inside and the outside of a water barrel, relates to automatic cleaning equipment for a pure water barrel, and more particularly relates to an automatic water barrel cleaning machine for simultaneously cleaning the inside and the outside. The problems that all domestic barreled pure water production plants cannot clean recycled water barrels from inside to outside because such cleaning equipment does not exist so far, particularly bacteria easily grow in the water barrels to pollute the water barrels because of high temperature in summer and the like in the background technology are solved. The adopted technical scheme for solving the technical problems is that the novel automatic cleaning machine for the inside and the outside of the water barrel mainly comprises a cylinder, a conveying belt, an inner hair brush, an outer hair brush and the like; the work efficiency is improved; the equipment works eight hours per day, and 5,000 barrels for holding pure water can be cleaned and filled only by three to four persons; the hygienic quality is improved; the work environment is improved; fully closed production is achieved, and the barrels are not in contact with the persons and the outside world from cleaning, filling to packaging.

The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

The invention discloses a technology for carrying out fungus growing on loose tea. The technology comprises the following steps: (1) preparing a fermenting agent, namely, separating out eurotium cristatum from fuzhuan tea, and preparing to obtain the solid pure strain fermenting agent; (2) treating raw dark green tea raw material, namely, removing the impurities in the raw dark green tea, steaming, carrying out pile fermentation and then feeding tea juice; (3) carrying out steaming inoculation and fungus growing, namely, steaming the raw dark green tea with the tea juice, then immediately feeding the solid pure strain fermenting agent and inoculating; after that, putting the inoculated raw dark green tea into a fungus growing culture room, and carrying out fungus growing culture; and (4) drying and packaging, namely, drying after fungus growing, and directly packaging the dried loose tea. After the technology is adopted, the fungus growing time is shortened, the bacterial pollution rate can be reduced in the fungus growing process of the loose tea, the problems that the existing loose tea is hard in fungus growing and easy in bacterial growth can be solved, the fungus growing rate of the loose tea reaches up to 100%, and the tea is widespread in golden flowers on the surface and the granules are plump.

Methods of detecting bacterial contamination in a platelet concentrate are performed using a dynamic light scattering (DLS) instrument and a sample holder. A sample of platelet concentrate can be held vertically or horizontally in a capillary in the sample holder. Alternatively, novel platelet storage bags modified to include an optically translucent window can be held within another variant of the sample holder. Still alternatively, platelet storage bags having one or more tubes detachably appended to the bag can be used. A sample is drawn off into an appended tube for placement directly into the sample holder. This method provides a number of related, non-invasive techniques for detecting whether bacteria has contaminated a platelet concentrate. Contamination indicators include a population of particles different from platelets, microparticles or proteins, bad-quality platelets, i.e. low DLS score, and very high or very low scattering intensity.

The invention provides a method for preparing mesenchymal stem cells of menstrual blood. The method comprises steps as follows: female menstrual blood is collected and taken as a raw material and is subjected to sterile processing; stem cells in the menstrual blood are separated; the mesenchymal stem cells of the menstrual blood are cultured; the mesenchymal stem cells of the menstrual blood are cryopreserved; and the mesenchymal stem cells of the menstrual blood are recovered. The method has the technical characteristics as follows: the female menstrual blood is collected and stored in a preservative fluid; by means of a bacterial pollution prevention method, the pollution probability is reduced from a collection source, a collecting cup is repeatedly washed by sterile water, and the pollution probability is effectively reduced; with the adoption of a differential centrifugation method, bacteria in the menstrual blood are removed as far as possible; a sample is repeatedly separated by HES (hydroxyethyl starch), and the maximum quantity of mesenchymal stem cells of the menstrual blood can be obtained; a serum-free medium is utilized for culturing, components of animal origin are reduced, the cell performance is stable, the in-vitro long-term culturing process of the mesenchymal stem cells of the menstrual blood can be kept, and cellular morphology, multiplication capacity, MSC (mesenchymal stem cell) surface marker expression, differentiation capacity and the like are maintained. The method is simple, practical and convenient to operate, the maximum quantity of required stem cells can be obtained, and the stem cells are successfully cultured.

The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample.

Orthopedic and dental implants coated with an antibacterial coating and methods of making and using, are described herein. The implant can be coated with a hydrophobic, polycationic antibacterial polymer. The polymer can be covalently or non-covalently associated with the surface; however, in particular embodiments, the polymer is non-covalently associated with the surface. As shown in the examples below, clinical observations, digital radiography, and a battery of well-accepted ex vivo analytical methods show that the presence of a hydrophobic polycationic polymer coating, such as N,N-dodecyl,methyl-PEI coating on the surface of a metal implant, was effective in eliminating the clinical signs of infection in vivo in a large animal infection model. Moreover, the coated plates supported bone healing, and in fact decreased healing times, even in the presence of significant bacterial contamination compared to a control.

The invention discloses an edible film-coating freshness preservation agent used for megalobrama amblycephala, which belongs to the technical fields of the freshness preservation and the processing of aquatic products. The freshness preservation agent is a water solution prepared by dissolving the following components in water, wherein each component and the concentration thereof in the water solution are as follows: 14-16g / L of sodium alginate, 95-105g / L of glycerol and 40-60g / L of ascorbate. The invention also discloses a use method of the freshness preservation agent. After the film-coating freshness preservation agent is used for the film coating of the megalobrama amblycephala, a layer of film can be formed on the surface of the megalobrama amblycephala and acts as the functions of reducing bacterial pollution, prohibiting microbial growth, attenuating air oxidation and slowing down the lipid oxidation rate, and the effect of freshness preservation is obviously superior to that of an ordinary low-temperature preservation method. The use method of the film-coating freshness preservation agent is simple, the equipment investment is little, the operation site is small, and the film-coating freshness preservation agent can be suitable for industrial mass production.

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample.

The invention discloses a preservative for aquatic products, and is particularly suitable for fish products. The preservative is prepared by dissolving the following components in water: 50 to 75 weight percent of sodium hexametaphosphate, sodium tripolyphosphate and sodium pyrophosphate, 15 to 20 weight percent of sodium alginate, 5 to 10 weight percent of ascorbic acid and 2 to 10 weight percent of glycerol, wherein the weight ratio of the sodium hexametaphosphate to the sodium tripolyphosphate to the sodium pyrophosphate is 10:2:1. The coating preservative is used for forming a layer of film on the surfaces of the aquatic products after the aquatic products are coated to achieve the effects of reducing bacterial pollution, inhibiting the growth of microbes and weakening air oxidation, and a refreshment effect is obviously superior to that of the ordinary low-temperature preservation method. A using method for the preservative is simple, the preservative can be suitable for industrial batch production, the investment of equipment is low, and an operation site is small.

The invention provides a culture medium of euglena gracilis, which is added with different inorganic nutrient substances, in particular to nutrient components such as a mixture of ammonium chloride and urea, and the like. In an open culture process, the culture medium not only overcomes the defects that euglena gracilis is easy to pollute by microorganisms and bacteria so as to pollute the environment, and the like in the prior art, but also can ensure that the euglena gracilis quickly utilizes the inorganic nutrient components to accelerate cell division under the condition of not adding an organic carbon source. The culture medium of the invention shortens the grown cycle of the euglena gracilis by 2-5 days compared with a conventional culture medium and has a biomass accumulation being 1-1.5 times higher than the conventional culture medium. The culture medium removes the organic carbon source of the conventional culture medium and supplements the carbon source in the mode of CO2, thus the culture medium not only can achieve the purpose of avoiding microorganism pollution, but also can increase the capacity of absorbing the CO2 of the euglena gracilis. In the process of the mass production and culture of the euglena gracilis, the culture medium can adsorb a large amount of industrial CO2 waste gas, thereby having important meaning to energy saving, emission reduction and environmental protection.

The invention aims to provide nano modified polylactic acid with high toughness and good flame resistance and a preparation method thereof. The nano modified polylactic acid is prepared by mixing the following raw materials in percentage by weight: 45 to 94.6 percent of polylactic acid, 5 to 45 percent of nano particles, 0.2 to 5 percent of thermal stability and 0.2 to 5 percent of processing aid. The preparation method comprises the following steps of: A, putting the weighed polylactic acid, nano particles, thermal stability and processing aid into a high-speed mixer in a ratio and mixing the raw materials; and B, conveying the raw materials mixed in the step A to a double-screw extruder, and melting, extruding and granulating the raw materials under high-speed shearing, mixing and conveying of screws to obtain a composite material. The polylactic acid is modified by using the nano particles, so the impact resistance, flame resistance, ageing resistance and bacterial pollution resistance of the polylactic acid composite material can be improved, and the application range of the polylactic acid composite material is enlarged.

The invention relates to the technical field of medical appliance. Ventilator associated pneumonia results from inhalation of secretion with pathogene, wherein, bacterial pollution of gas circuit of the ventilator is a key factor which causes the ventilator associated pneumonia. The invention aims at providing an artificial trachea cannula with broad-spectrum antibacterium property and controllable slowly-releasing drugs; an air providing tube (2), an air bag (4), an air tube (3) and a liquid discharging tube (5) of the artificial trachea cannula of the invention are coated with nanometre anti-microbial coatings (6) on inner and outer surfaces; the nanometre anti-microbial coatings are mainly blended by macromolecule polymers, nanometre inorganic antimicrobial and slowly-releasing drugs. The artificial trachea cannula with nanometre anti-microbial coatings of the invention not only satisfies traditional functions of artificial trachea in providing air path and maintaining respiratory tract clear, but also has functions of excellent bacteriostasis and slowly releasing of controllable drugs, thereby providing wide application prospect to clinical application of the artificial trachea cannula.

The invention relates to a current enzyme electrode for detecting catalase-positive bacteria and a preparation method thereof in the technical field of biological sensing; the electrode comprises an electrode substrate, wiring terminals, electrode connection cables, a working electrode, a counter electrode, an insulation layer and reaction substrate films, and is characterized in that the wiring terminals, the electrode connection cables, the working electrode and the counter electrode are respectively arranged between the electrode substrate and the insulation layer; the working electrode is arranged in the middle of the electrode with a gap left between; one end of each electrode connection cable is respectively connected with the working electrode and the counter electrode, and the other end thereof is respectively connected with the two wiring terminals; and the reaction substrate films are arranged above the working electrode and the counter electrode. The electrode which is prepared by the method has the advantages of high sensitivity and no need of bacterial culturing, can realize continuous detection of a plurality of samples by replacing different disposable electrode strips, is simple to operate, is easy for industrial popularization, and has important practical value in food bacterial pollution monitoring.

The invention discloses a plant source extract anti-bacterial preservative film, and a preparation method and application thereof. According to the method, boracic acid and hydrochloric acid are used for modifying vinol; then, the modified vinol is uniformly mixed with nanometer titania; next, the mixture is uniformly mixed with crofton weed ethanol extracts emulsified by tween-80; film casting is performed; the materials are dried under the natural conditions; the plant source extract anti-bacterial preservative film is obtained. The film has the proper oxygen permeability and moisture permeability; due to plant source extracts contained in the preservative film, preserved articles can be prevented from getting rotten through being infected by bacteria; ethylene gas generated by fruit and vegetables can be degraded by nanometer materials; meanwhile, the film also has excellent mechanical property. The plant source extract anti-bacterial preservative film is mainly applied to the fields of preservative package of fruit and vegetables, sea products and cereal subsidiary food and the like, and can also be applied to food container coverage, so that the food is prevented from getting rotten through being polluted by bacteria; the storage life is prolonged.

A process for preparing the injection of carnosine features that the chitosan and oligose are used for adsorbing the modified protein and fat to improve the purity of product, and the 6-aminopurine phosphate is used as the protector for increasing the sterilizing temp and time to 121 deg.C and 30 min, so having no bacterial pollution.

The invention relates to a medical cotton swab asepsis storage box, and discloses a storage and taking device through which cotton swabs can be conveniently taken out and bacterial pollution can be effectively prevented. The medical cotton swab asepsis storage box is characterized in that a fixing frame is arranged on upper edges on the left side and the right side of a main box through fixing grooves, a limiting plate is arranged on the fixing frame and parallel to the bottom of the main box, a cover plate is arranged on the main box and located below the limiting plate, the cover plate is provided with a taking outlet, a taking-out cover is hinged to the portion, on one side of the taking outlet, of the cover plate through a connection strip and located above the taking outlet, multiple partition plates are distributed on the cover plate at equal intervals and located in the main body, the distance between every two adjacent partition plates is slightly larger than the diameter of each cotton swab, multiple baffles are evenly distributed on the taking-out cover and arranged between the partition plates in a one-to-one corresponding mode, the taking outlet is divided by the baffles into multiple small windows, multiple shifting needles are evenly arranged on the cover plate and located between the partition plates correspondingly, and the shifting needles are located on one side of the taking outlet.

A beverage bottle boxing machine comprises a rack, a rotating disc driven by a driving device to rotate is installed in the center of the rack, multiple processing stations for containing packaging boxes are concavely formed in the rotating disc, the periphery of the rotating disc is sequentially connected with a packaging box loading device, a beverage bottle feeding and upper cap loading device, a lower cap loading device, a lower pressing device, a bottle cap loading device, a bottle cap screwing device and a discharging output device, and the processing stations sequentially pass through the devices along with rotation of the rotating disc. The beverage bottle boxing machine is high in automation degree, production efficiency is improved, labor is saved, cost is reduced, bacterial pollution caused by manual contact in the boxing process is reduced, and the beverage bottle boxing machine is suitable for wide application and popularization.

The invention discloses a method for tissue culture and rapid propagation of stems of cinnamomum kanehirae hay. The method comprises the steps of S1. disinfecting explants of the stems of cinnamomum kanehirae hay, inoculating the disinfected explants into an induction medium, and carrying out induction culture; S2. carrying out proliferating cultivation, namely, sequentially inoculating the explants subjected to induction culture into a proliferation medium A and a proliferation medium B, and carrying out proliferating cultivation; S3. carrying out strong seedling culture; and S4. carrying out rooting culture. According to the method, the conditions that fungus and bacterial pollution is easily caused to the explants of cinnamomum kanehirae hay in the primary culture process can be avoided, and the germination rate can be ensured while the pollution rate is reduced. Moreover, the concentration of the hormone of the proliferation medium A is slightly high, so that calluses can be induced; the calluses are inoculated to the proliferation medium B with relatively low hormone concentration, withering and death of leaves can be reduced, a large quantity of calluses can be differentiated, the month propagation coefficient achieves 4-6 times, the form is normal in the propagation process, large-scale industrial production can be met, and the nursery stock breeding efficiency can be greatly improved.

The present invention relates to a method so called Bacterial Digitalcode System (BaDis) that identifies microorganism by using bacterial-specific, genus-specific and species-specific oligonucleotides from a variety of samples or specimens for detection and differential diagnosis of microorganism. Particularly, the present invention relates to bacterial-specific, genus-specific and species-specific oligonucleotides designed by the target nucleotide sequences of 23S rDNA or ITS gene, polymerase chain reaction (hereinafter, referred to as “PCR”) kits using the oligonucleotides as a primer, the microarray containing the oligonucleotides as a probe, and methods for detecting microorganism by using the oligonucleotides. Therefore, the present invention can be applied to detect the presence of microorganism and diagnose differentially all microorganism such as pathogenic bacteria of infectious diseases, bacteria inducing food poisoning, bacteria contaminating biomedical products and environmental pollutants.

A smallpeptide chelate trace element feed additive comprises, in mass percent, from 18 to 89% of smallpeptide protein, from 10 to 20% of salt and from 0.59 to 62% of inorganic trace element when in a dry state. A production process includes following steps of (1) concentrating protein hydrolysate waste liquor which has the solid content of 5-15% and is generated from production of chondroitin and heparin sodium into protein hydrolysate waste liquor with the solid content of 30-40%; (2) adding prepared single monomial or compounded inorganic trace element solution with the mass percent concentration of 15-35%, controlling a pH (potential of hydrogen) value to be 6-8, and performing chelation reaction; and (3) performing spraying drying at the temperature ranging from 180 DEG C to 220 DEG C. The smallpeptide chelate trace element feed additive and the production process thereof effectively avoid waste liquor pollution generated in a production process of the chondroitin and heparin sodium, and turn waste into wealth, and prepared organic chelate feed additive enables the additional value of the waste liquor to be maximized. Spraying drying technology is adopted for the production process, and the problem that drying time is long and products are easy to be polluted by bacteria in a common method is solved.

The invention discloses a household garbage treatment method and a household garbage treatment device. The method comprises the following steps of: (1) performing magnetic separation, performing magnetic separation on household garbage by using magnetic separation equipment so as to remove ironware from the household garbage; (2) drying and sterilizing at high temperature: introducing the garbage subjected to magnetic separation into a high-temperature drying and sterilizing device at the temperature of between 80 and 200 DEG C for drying and sterilizing at high temperature; (3) adding a chemical aid: adding a dry powder chemical aid into the dried and sterilized garbage; (4) crushing: crushing the garbage in the step (3) by using a crusher; (5) stirring: uniformly stirring the garbage in the step (4) by using a stirrer; and (6) performing hot-pressing to form garbage carbon: performing hot-pressing on the garbage treated in the step (5) to form the garbage carbon by using hot pressing equipment. The household garbage treatment method has the advantages that: sewage pollution, odor pollution, animal pollution such as flies and bacterial pollution are avoided; the garbage is treated to become new energy; and the aims of harmless garbage treatment, recycling of garbage, and reduction of garbage are fulfilled.

Bacterial contamination of industrial water systems lead to biofouling by biofilms and corrosion from bacterial induced corrosion. This invention provides a method for control of fouling and contamination of industrial water systems caused by bacteria. Prevention or reduction of process interruptions and general contamination, fouling and corrosion is achieved by the destruction of targeted problematic bacteria with naturally occurring, non-engineered bacteriophage virulent for targeted bacteria. The invention also provides for in-situ confirmation of the proper identification of target bacteria and a mobile laboratory adapted to implement the method.

The invention relates to a sterilizing method for liquid food. Carrying out UHT (ultrahigh temperature) or high-temperature sterilization and reduced temperature filling to the liquid food, then carrying out sterilization through a special microwave sterilization device, and then cooling the sterilized liquid food to room temperature to obtain the finished product; when the filling temperature decreases to 25-65 DEG C, carrying out microwave sterilization immediately; and particularly when the filling temperature decreases to 40 DEG C plus or minus 5 DEG C, carrying out microwave sterilization immediately. The UHT or high-temperature sterilization is carried out at a temperature of 121 DEG C above, the microwave sterilization temperature can be lower, according to the method and the device, the microwave sterilization after filling can be carried out to the non-high-temperature soft bags, plastic bottles, glass bottles and other nonmetal bottles at a higher temperature, the general disinfection is required to be carried out to the pipes, bottles, bottle caps, air and the like, the cost is low, the management difficulty is low, and the secondary bacterial pollution is avoided due to the sterilization after filling.

The invention discloses a preparation method of porous nano antibacterial particles and a composite nanofiltration membrane and the composite nanofiltration membrane. The preparation method of the porous nano-antibacterial particle composite nanofiltration membrane comprises the following steps: 1, adding porous copper-coated TiO2 nano-antibacterial particles into a basic solution, and uniformly embedding the porous nano-antibacterial particles into the basic solution through ultrasonic dispersion or strong stirring to prepare a monomer solution; wherein the basic solution is a polybasic acylchloride solution and a piperazine aqueous solution, or a polybasic acyl chloride solution and an aqueous solution of a mixture of piperazine and polyamine; and 2, carrying out interfacial polymerization reaction on the monomer solution on the surface of an ultrafiltration support membrane layer to obtain the porous copper-coated TiO2 nano antibacterial particle composite nanofiltration membrane.The prepared porous copper-coated titanium dioxide nano antibacterial particle composite nanofiltration membrane is large in water flux, high in desalination rate, resistant to bacterial pollution, easy to clean and capable of being widely applied to the application fields of sewage treatment, material concentration, decoloration, desalination of alkaline water or seawater and the like.