Technology for carrying out fungus growing on loose tea
A process and tea juice technology, applied in the field of loose tea blooming process, can solve the problems of inability to guarantee the growth advantage of Coronoid spp., uncontrollable tea blooming, destroying the activity of golden flower fungus, etc., so as to shorten the blooming time. , the effect of shortening the blooming cycle and improving the safety and convenience of drinking
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[0024] 1) Preparation of leavening agent:
[0025] ① Firstly, isolate S. coronoidis from Fuzhuan tea, purify and identify it, store it on a low-temperature slant, and use PDA to expand and cultivate it for later use;
[0026] 2. prepare starter culture medium then, starter culture medium comprises the tea that tea-water ratio is 1 / 40, every 100L tea water adds bran 100kg, glucose 6kg, yeast extract powder 4kg, NaCl 0.8kg; Described starter culture medium is at 121 Sterilize for 30 minutes at a temperature of ℃ or at a pressure of 0.1-0.15 MPa;
[0027] ③The expanded and cultivated S. coronoidis prepared at a concentration of 2×10 7 ~9×10 7 Bacteria solution per mL, inoculated on the above medium, the inoculum amount is 20ml / kg relative to the above medium, the culture time is 8 days, the culture temperature is 27-29°C for the first three days, and 25-28°C for the next five days , when the golden-yellow closed cyst shells completely cover the surface of the culture medium, l...
Embodiment 1
[0041] A process for developing loose tea flowers, comprising the following steps:
[0042] 1) Preparation of leavening agent:
[0043] ① Firstly, isolate S. coronoidis from Fuzhuan tea, purify and identify it, store it on a low-temperature slant, and use PDA to expand and cultivate it for later use;
[0044] 2. prepare starter culture medium then, starter culture medium comprises the tea that tea-water ratio is 1 / 40, every 100L tea water adds bran 100kg, glucose 6kg, yeast extract powder 4kg, NaCl 0.8kg; Described starter culture medium is at 121 Sterilize for 30 minutes at a temperature of ℃ or a pressure of 0.1 MPa;
[0045] ③The expanded and cultivated S. coronoidis prepared at a concentration of 2×10 7 ~9×10 7 bacteria solution per mL, inoculated on the above medium, the inoculum amount is 20ml / kg relative to the above medium, the culture time is 8 days, the culture temperature is 27°C for the first three days, and 25°C for the next five days, when golden yellow When ...
Embodiment 2
[0051] A process for developing loose tea flowers, comprising the following steps:
[0052] 1) Preparation of leavening agent:
[0053] ① Firstly, isolate S. coronoidis from Fuzhuan tea, purify and identify it, store it on a low-temperature slant, and use PDA to expand and cultivate it for later use;
[0054] 2. prepare starter culture medium then, starter culture medium comprises the tea that tea-water ratio is 1 / 40, every 100L tea water adds bran 100kg, glucose 6kg, yeast extract powder 4kg, NaCl 0.8kg; Described starter culture medium is at 121 Sterilize for 30 minutes at a temperature of ℃ or a pressure of 0.12 MPa;
[0055] ③The expanded and cultivated S. coronoidis prepared at a concentration of 2×10 7 ~9×10 7 Bacteria solution per mL, inoculated on the above-mentioned medium, the inoculum amount is 20ml / kg relative to the above-mentioned medium, the culture time is 8 days, the culture temperature is 28°C for the first three days, and 26°C for the next five days, when...
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