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Nocardia rubra-cell wall skeleton solvent extraction method

A technology of Nocardia rubrum and extraction method, which is applied in the field of microbial pharmaceuticals, can solve problems such as destruction, personnel damage to the environment, and hidden dangers left by residues, and achieve the effects of ensuring product quality, reducing extraction time, and reducing residues

Active Publication Date: 2017-10-10
FUJIAN SHANHE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the solvent extraction process is: "ethanol, ether, chloroform, methanol separate or mixed step-by-step extraction", the methanol, ether and chloroform used in this process are poisonous and harmful organic solvents, and there may be There are trace residues that leave hidden dangers, and may cause harm to personnel and damage to the environment during the operation process, and further improvement is needed

Method used

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  • Nocardia rubra-cell wall skeleton solvent extraction method
  • Nocardia rubra-cell wall skeleton solvent extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting a Nocardia rubra cell wall skeleton solvent, comprising the following steps:

[0027] (1), ethyl acetate is added in the cell wall skeleton fragments of Nocardia rubra obtained after enzymolysis according to the ratio of ethyl acetate and Nocardia rubra cell wall skeleton fragments as 6ml: 1g, to normal temperature, magnetic stirring for 5 Hours, centrifugation: 3136g, 8°C, 20min, discard the supernatant and keep the precipitated debris;

[0028] (2), add ethyl acetate to the precipitated fragments obtained in step (1) and centrifuge them, stir them magnetically for 16 hours at room temperature, centrifuge: 3136g, 8°C, 20min, discard the supernatant, and leave the precipitated fragments, wherein, The ratio of ethyl acetate to the precipitated fragments obtained in step (1) is 6ml: 1g;

[0029] (3) Add absolute ethanol to the precipitated debris obtained in step (2) at a ratio of 6ml: 1g to the precipitated debris obtained in step (2), stir evenl...

Embodiment 2

[0034] A method for extracting a Nocardia rubra cell wall skeleton solvent, comprising the following steps:

[0035] (1), ethyl acetate is added in the cell wall skeleton fragments of Nocardia rubra obtained after enzymolysis according to the ratio of ethyl acetate and Nocardia rubra cell wall skeleton fragments as 5ml: 1g, to normal temperature, magnetic stirring for 7 Hours, centrifugation: 3136g, 8°C, 20min, discard the supernatant and keep the precipitated debris;

[0036] (2), add ethyl acetate to the precipitated fragments obtained in step (1) and centrifuge them, stir them magnetically for 15 hours at room temperature, centrifuge: 3136g, 8°C, 20min, discard the supernatant, and leave the precipitated fragments, wherein, The ratio of ethyl acetate to the precipitated fragments obtained in step (1) is 5ml: 1g;

[0037] (3) Add absolute ethanol to the precipitated debris obtained in step (2) at a ratio of 5ml: 1g to the precipitated debris obtained in step (2) and stir ev...

Embodiment 3

[0042] A method for extracting a Nocardia rubra cell wall skeleton solvent, comprising the following steps:

[0043] (1), add ethyl acetate to the cell wall skeleton fragments of Nocardia rubra obtained after enzymolysis according to the ratio of ethyl acetate and Nocardia rubra cell wall skeleton fragments as 8ml: 1g, and stir magnetically at room temperature for 6 Hours, centrifugation: 3136g, 8°C, 20min, discard the supernatant and keep the precipitated debris;

[0044] (2) Add ethyl acetate to the precipitated fragments obtained in step (1) and centrifuge them, stir magnetically for 18 hours at room temperature, centrifuge: 3136g, 8°C, 20min, discard the supernatant, and leave the precipitated fragments, wherein, The ratio of ethyl acetate to the precipitated fragments obtained in step (1) is 8ml: 1g;

[0045] (3) Add absolute ethanol to the precipitated debris obtained in step (2) at a ratio of 8ml: 1g to the precipitated debris obtained in step (2) and stir evenly, wash...

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Abstract

The invention discloses a nocardia rubra-cell wall skeleton solvent extraction method which is characterized by including the steps: (1) ethyl acetate extraction: adding ethyl acetate in nocardia rubra-cell wall skeleton fragments acquired after enzymolysis, stirring mixture for 5-18 hours at the indoor temperature, performing centrifuging treatment, abandoning liquid supernatant, and reserving sedimentation fragments; (2) absolute ethyl alcohol stirring and cleaning: adding the absolute ethyl alcohol into the sedimentation fragments acquired in the step (1) to uniformly stir mixture, performing centrifuging treatment, abandoning liquid supernatant, and reserving sedimentation fragments; (3) absolute ethyl alcohol extraction: adding the absolute ethyl alcohol into the sedimentation fragments acquired in the step (2), stirring mixture for 5-18 hours at the indoor temperature, performing centrifuging treatment, and abandoning liquid supernatant to obtain final sedimentation fragments. The proportion of the nocardia rubra-cell wall skeleton fragments and ethyl acetate is 1g: 5-8ml, and the proportion of the sedimentation fragments acquired in the step (2) and the absolute ethyl alcohol is 1g:5-8ml.

Description

technical field [0001] The invention belongs to the technical field of microbial pharmacy, and in particular relates to a solvent extraction method for the cell wall skeleton of Nocardia rubrum. Background technique [0002] Nocardia rubra (Nocardia rubra) is an immunologically active microorganism. It obtains its cell wall skeleton through cell crushing, removal of cell contents, proteins and lipids and other components. The active substances of neutral polysaccharides and mucopeptides can stimulate cellular immune function, promote the secretion of various cytokines, participate in the anti-tumor immune process, and are used for the treatment or adjuvant therapy of various tumors; they can also be used for the development of new dosage forms and new adaptations Diseases such as skin lesions and gastrointestinal diseases. [0003] In the prior art, the solvent extraction process is: "ethanol, ether, chloroform, methanol separate or mixed step-by-step extraction", the metha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P37/04A61P35/00
CPCA61K35/74
Inventor 徐镜黄春玉龚丽萍黄建
Owner FUJIAN SHANHE PHARMA
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