33 results about "Netilmicin" patented technology

The invention relates to a separation and purification method of high-purity netilmicin, belonging to the technical fields of medical continuous chromatographic separation and binding film separation and purification. In the invention, the continuous chromatographic separation technology is used for separating and purifying triacetyl sisomicin reaction liquid to obtain high-purity triacetyl sisomicin, and then, the industrial chromatographic separation and film separation combined technology is used for purifying and separating netilmicin hydrolyzate to obtain high-purity netilmicin. By adopting a continuous chromatographic separation system combined with film ultrafiltration and nanofiltration technology, the feed liquid of the triacetyl sisomicin and the netilmicin which are used as intermediates can be effectively separated and purified through the continuous chromatographic separation system; in the film separation production process, the reasonable temperature condition is controlled to reduce the generation of degradation products; and meanwhile, the production cost can be reduced, the production cycle can be shortened, the product quality can be improved, and the discharge amount of waste water can be lowered. The netilmicin purity of the product, which is measured by the high performance liquid chromatography (HPLC) method, is greater than or equal to 98%.

The invention discloses a chemical medicine composition for treating helicobacter pylori (Hp) infection of adults through oral administration. The composition at least comprises fosfomycin or pharmaceutical salts such as a sodium salt, a calcium salt, fosfomycin trometamol and aminoglycoside medicines of the fosfomycin of a common dosage of an adult, and the aminoglycoside medicines are selected from tobramycin, netilmicin, etimicin, isepamicin, amikacin and common salts such as sulfate, of the medicines.

The invention discloses a method for simultaneously separation determination of the content of three aminoglycoside antibiotics (AGs) (tobramycin, etimicin and netilmicin); the method successfully separates a mixture of the three antibiotics, and also is applied in determination of the content of the AGs in tobramycin eye drops, an etimicin sulfate injection and a netilmicin sulfate injection, and an HPLC-RRS combined technology is used for determination of the AGs. In an experiment, used chromatographic columns comprise Agilent Zorbox SB-C18 column (250 mm*4.6 mm i.d., 5 [mu]m) and a protective column (Security Guard C18, 4*2 mm, Phenomenex). A mobile phase is methanol:water (containing 0.2% TFA)=5:95 (v/v), the HPLC flow rate is 0.50 mL*min<-1>, [lambda]ex=[lambda]em=362 nm, the column temperature is 30 DEG C, and the sample injection volume is 20 [mu]L. The probe is a 2.0*10<-5> mol*L<-1> PSB solution, the pH is 9.5, the single-pump flow rate is 0.2 mL*min<-1>, the reaction pipe length is 250 cm, and the internal diameter is 0.5 mm. A brand-new HPLC detection mode established with RRS as a detection method is established, and is successfully applied to determination of the content of the AGs in different preparations. The application range of the RRS technology is widened, and the brand-new detection mode is provided for HPLC.

The invention describes an antibiotic coating for porous bodies and its use. Into the porous system of non-metallic porous bodies and of metallic porous bodies, a coating made of at least one antibiotic salt that is hardly soluble in water or in an aqueous environment from the group of the netilmicin laurate, the netilmicin myristate, the netilmicin dodecyl sulfate, the sisomicin laurate, the sisomicin myristate, the sisomicin dodecyl sulfate, the gentamicin laurate, the gentamicin myristate, the clindamycin laurate, the amikacin laurate, the amikacin myristate, the amikacin dodecyl sulfate, the kanamycin laurate, the kanamycin myristate, the kanamycin dodecyl sulfate, the tobramycin laurate, the tobramycin myristate, the tobramycin dodecyl sulfate, the ciprofloxacin myristate, the vancomycin dodecyl sulfate, the vancomycin laurate, the vancomycin myristate, the vancomycin teicoplanin and the clindamycin teicoplanin is introduced. The antibiotically coated, porous bodies are used as implants.

The invention relates to a netilmicin sulfate injection and a preparing method. Particularly, the netilmicin sulfate injection is prepared from netilmicin sulfate, sodium sulfite or sodium hydrogen sulfite, cysteine, edetate disodium or sodium calcium edentate and water for injection. The invention further relates to the preparing method for the netilmicin sulfate injection. The prepared netilmicin sulfate injection has one or more excellent characteristics described in the description.

The invention discloses a Netilmicin liposome and the preparation technique. The Netilmicin liposome is composed by the following materials in weight ratio: one share of Netilmicin, two to seven shares of cholesterol and four to eighteen shares of lecithin. The preparation technique includes the infusing and ultrasonic method and the multiple emulsion and ultrasonic method, with the following steps: firstly, dissolve a matched proportion of Netilmicin medicine into a neutral-pH buffer solution of phosphate as an aqueous phase; secondly, dissolve a matched proportion of the cholesterol and the lecithin into a proper proportion of Ethyl ether as an organic phase; thirdly, drop the organic phase into the aqueous phase uniformly and stir simultaneously; and finally with the water ultrasonic extraction method, an uniform emulsion, the Netilmicin liposome with a particle diameter of one hundred to two hundreds nanometers, is formed with renal toxicity decreased greatly. The invention has the advantages that due to simplified operation of processing, the liposome with less particle diameter and better stability can be formed; the particle diameter is notably reduced while the uniformity is improved; additionally, the envelope rate rises to fifty percents or above.

The invention discloses a netilmicin immunoassay reagent and preparation and detection method thereof. The netilmicin immunoassay reagent includes enzyme-labeled netilmicin and an indicator reagent for detection of a netilmicin antibody-enzyme-labeled netilmicin complex; the enzyme-labeled netilmicin is conjugated by netilmicin and glucose dehydrogenase. The netilmicin immunoassay reagent can accurately and rapidly determine the content of the netilmicin in human blood and other samples. Compared with the existing detection reagents on the market, the netilmicin immunoassay reagent has the advantages of convenience, rapidity, high sensitivity, strong specificity, accurate quantification and so on, and is conducive to clinical popularization and use.

The invention discloses a western medicine composition for treating leucoderma. The composition is prepared from, by weight, 0.1-1 part of vitamin C, 0.1-2 parts of vitamin p, 0.1-1 part of vitamin E, 2-5 parts of placentin, 2-8 parts of hair, 10-25 parts of thymosin injection, 0.2-5 parts of retinoic acid, 1-3 parts of netilmicin, 2-5 parts of acetylspiramycin, 2-10 parts of pazufloxacin mesilate, 1-5 parts of asparaginic acid, 0.5-1 part of glycine, 2-5 parts of compound kaliziran tincture and 1-5 parts of vindesine. The medicine for treating leucoderma has the advantages that the medicine is good in treatment effect, takes effect quickly and is short in treatment period and small in toxic and side effects, leucoderma is not likely to relapse after drug withdrawal, and the medicine is convenient to take. Formation of melanin around a leukoplakia portion is benefited, and the effective rate is as high as 80%. A preparation method is simple, cost is low, and economic burden of patients is effectively reduced.

The invention provides a fructose injection of an antibiotic drug. The injection is composed of antibiotics, fructose and water. The injection also can contain proper additives. The antibiotic comprise gatifloxacin, levofloxacin, ofloxacin, ciprofloxacin, pazufloxacin, fleroxacin, sparfloxacin, moxifloxacin, pefloxacin, rufloxacin, lomefloxacin, norfloxacin, caderofloxacin, azithromycin, telithromycin, ornidazole, secnidazole, tinidazole, metronidazole, clindamycin, lincomycin, fluconazole, etimicin, netilmicin, amikacin and medicinal acid additive salts, esterified compounds, derivatives and the like. The antibiotics are prepared into the fructose injection. Besides the advantages of convenience in use and quickness to take effect of the injection, compared with a gluconic infection, the injection provided by the invention is more easily absorbed and utilized, is more suitable for preventing bacteria and diminishing inflammation, supplying energy and supplementing body fluids for patients with diabetes, heart disease and liver disease, so that the application range of the injection is expanded.

The invention discloses a preparation method of netilmicin sulfate sterile powder injection for injection, which comprises the following steps: taking water for injection, adding an antioxidant and an excipient, and conducting dissolving to obtain a first solution; cooling the first solution to 5-15 DEG C, and adding netilmicin sulfate for dissolving to obtain a second solution; adjusting the pH value of the second solution to 5.0-5.5 by using a pH value regulator to obtain a third solution; decoloring, degerming and filtering the third solution to obtain a fourth solution; and freezing and drying the fourth solution to obtain the netilmicin sulfate sterile powder injection. The netilmicin sulfate freeze-dried powder injection prepared by the invention has the beneficial effects that the stability is good, the content of active ingredients of the product is high, the resolubility is excellent, and the long-term storage is facilitated.

The invention provides a method for detecting the residual amount of mercury in netilmicin sulfate, which comprises the following five steps: (1) preparing a linear stock solution and a linear solution from a mercury element; (2) preparing a test solution; (3) preparing a standard test solution; (4) measuring the linear solution in ICP, recording a spectrogram, and carrying out linear regression by taking the sample injection concentration as an abscissa and the peak area as an ordinate to obtain a regression equation; and (5) sequentially determining the test solution and the standard test solution according to the steps (2) and (3), and determining the repeatability intermediate precision and the recovery rate of the standard test solution. According to the method disclosed by the invention, the efficient determination of the residual quantity of Cd, Pb, As, Co, V and Ni in netilmicin sulfate is realized through five steps, the operation steps are simple, the sensitivity is good, and the safety, effectiveness and quality controllability of medicines are ensured.

A sustained-release injection containing netilmicin comprises sustained-release microspheres and solvent. The microspheres contain sustained-release adjuvants and aminoglycoside antibiotics, and the solvent is a special solvent containing suspending agent such as sodium carboxymethylcellulose and having viscosity of 100-3000cp (20-30 DEG C); and the sustained-release adjuvants are selected from poly(ethylene-co-vinylacetate) (EVAc), polifeprosan, poly(lactic acid) (PLA), poly(lactic-co-glycolic acid) (PLGA), sebacic acid copolymer, albumin glue and gelatin; the sustained-release microspheres can also be made into sustained-release implant or ointment. The sustained-release implant or injection can be locally placed or injected into foci to locally sustained-release of drug for 5-30 days, so as to obtain and maintain local drug effective concentration while remarkably reduce the systemic toxicity of the drug. The sustained-release injection has distinct and unique therapeutic effect on local infection diseases caused by Staphylococci, Streptococci, Peptostreptococcus, Propionibacterium acnes, Enterobacter, Mycobacterium tuberculosis, Gonococcus or Meningococcus, such as chronic osteomyelitis, severe decubital ulcer, refractory skin ulcer, diabetic foot, femoral head necrosis, abscess, etc.

The invention provides a netilmicin sulfate aerosol. The netilmicin sulfate aerosol is prepared from the following raw materials: 5 to 10g of netilmicin sulfate, 100 to 200ml of solvent, a propellant and 0.05 to 0.5 g of additive. According to the netilmicin sulfate aerosol provided by the invention, the medicine can directly reach the acting part, the netilmicin sulfate aerosol is uniform in distribution, quick in action, convenient to use, free of drinking water, suitable for people of all ages and beneficial to improving the medication compliance of patients, and the netilmicin sulfate aerosol can be pressed once. On the other hand, due to the fact that the medicine is used for local action, the administration dosage is obviously reduced, and the occurrence rate of side effects such as ototoxicity and renal toxicity is reduced.

A fructose injection of an antibiotic drug comprises antibiotics, fructose and water and can contain proper additives. The antibiotics comprise gatifloxacin, levofloxacin, ofloxacin, ciprofloxacin, pazufloxacin, fleroxacin, sparfloxacin, moxifloxacin, pefloxacin, rufloxacin, lomefloxacin, norfloxacin, cadrofloxacin, azithromycin, telithromycin, ornidazole, secnidazole, tinidazole, metronidazole, clindamycin, lincomycin, fluconazole, etimicin, netilmicin, amikacin as well as pharmaceutical acid added salts, esters and derivatives thereof. The antibiotics are prepared into the fructose injection, beside injection advantages of being convenient to use and taking effect quickly, compared with glucose injections, the injection is easier to absorb and utilize and more suitable for antisepsis andanti-inflammation, energy supply and body fluid supplement use for patients suffering from diabetes, heart disease and hepatopathy, and the use range of the injection is enlarged.

The invention discloses eye drops containing netilmicin sulfate. Every 1000ml of the eye drops comprises the following components: 1-10g of netilmicin sulfate, 60-80g of a preservative, 1-10g of an isoosmotic adjusting agent and the balance of purified water. In the eye drops provided by the invention, netilmicin sulfate is used as a main drug, and the preservative, the isoosmotic adjusting agentand the purified water are used as auxiliary materials. The whole preparation process is simple, the production efficiency is high, and the eye drops can be used for treating various eye infections sensitive to netilmicin.

The present invention concerns the use of a compound comprising one or more sugar moieties, which optionally are aminosubstitutes, and possibly other moieties, wherein said compound is a molecule comprising at least two aminosubstituents, said aminosubstituents being primary, secondary or tertiary amino groups, wherein said aminosubstituents are either attached to one single sugar moiety or attached to several sugar moieties or other moieties of the molecule, or to chains connecting two moieties or to chains being substituents to the molecule, for the manufacture of a pharmaceutical preparation useful as an agent capable of influencing an amine oxidase enzyme activity, more particularly inhibiting VAP-1/SSAO activity. The preferred compounds are aminoglycoside antibiotics, e.g., streptomycin, netilmicin, geneticin, gentamicin, puromycin, tobramycin and amikacin. The preferred conditions to be treated are inflammatory diseases or conditions, diseases related to carbohydrate metabolism, diseases related to aberrations in adipocyte differentiation or function or smooth muscle cell function, and vascular diseases.