44 results about "Lomefloxacin" patented technology

The invention relates to a method for splitting a chiral medicine of hydrochloric acid lomefloxacin, adopting a high-speed counter-current chromatography method to separate out levorotatory hydrochloric acid lomefloxacin and dextrorotatory hydrochloric acid lomefloxacin from a hydrochloric acid lomefloxacin crude extract. A solvent system comprises three components of ethers or normal paraffin hydrocarbon or fatty ester, acetonitrile or fatty alcohol or fatty ketone and water, the volume ratio is sequentially 5-100:1:5-100, an upper phase is used as a stationary phase, a lower phase is used as a flowing phase, sulphonation-beta-schardinger dextrin is used as a chiral splitting reagent, the content of the sulphonation-beta-schardinger dextrin in the lower phase is 0.02-0.06mol / L, and a chiral flowing phase method is adopted to split a hydrochloric acid lomefloxacin enantiomorph. The method for splitting the chiral medicine of the hydrochloric acid lomefloxacin can achieve higher separating degree, is suitable for various types of prepared type counter-current chromatography instruments and can separate and obtain the monomers of the levorotatory hydrochloric acid lomefloxacin and the dextrorotatory hydrochloric acid lomefloxacin.

The invention belongs to the technical field of molecularly imprinted polymers, and particularly discloses a magnetic carboxylation nano-crystalline cellulose amino-functionalization surface molecularly imprinted polymer. Magnetic carboxylation nano-crystalline cellulose Fe3O4@CCNs serves as a carrier, ofloxacin OFX serves as template molecules, glycidyl methacrylate GMA serves as a functional monomer, and through a polymerization reaction, the magnetic carboxylation nano-crystalline cellulose amino-functionalization surface molecularly imprinted polymer Fe3O4@CCNs@MIPs is formed. Fluoroquinolone drugs in a water body can be selectively extracted, and include ofloxacin OFX, lomefloxacin LOM, gatifloxacin GAT, sarafloxacin SARA, marbofloxacin MARBO, orbifloxacin OBX, difloxacin DFX, ciprofloxacin CIP, enrofloxacin ENRO, sparfloxacin SPX, and moxifloxacin MFX, the recovery rate reaches 81.2%-93.7%, and the adsorption capacity reaches 33 mg / g after adsorption / elution are repeated seven times.

The invention relates to a lomefloxacin hydrochloride injection and the preparation method, the components and the parts by weight of the injection are: 5 to 15 weight parts of lomefloxacin hydrochloride; 150 to 250 weight parts of DMF; 10 to 30 parts of Tween-80; 5 to 15 weight parts of nicotinamide and 600 to 850 weight parts of injection water. The steps of the preparation method are as follows: (1) the lomefloxacin hydrochloride is weighed according to the weight parts, and then the DMF is added; (2) Tween-80 is added according to the weight parts; (3) the nicotinamide is added according to the weight parts; (4) the injection water is added according to the weight parts; (5) the pH value is adjusted to 3.5 to 5.5, and the injection water is added till 1000 weight parts; (6) 0.5 to 2 weight parts of activated carbon for needle use is added, the mixture is placed still for 10 to 30 minutes, the filtration and filling-sealing are carried out, and the mixture is sterilized at 80 to 120 DEG C for 20 to 40 minutes, so as to prepare the lomefloxacin hydrochloride injection finished product. The product of the invention has good stability, exact efficacy, difficult decomposition and precipitation; furthermore, the invention can realize large-scale industrial production.

The invention discloses a method and special immune affinity adsorbent for extracting quinolone compound and / or sulfonamide compound. The immune affinity adsorbent consists of a solid-phase carrier and Norfloxacin monoclonal antibody and / or sulfamethoxazole monoclonal antibody coupled with the carrier, wherein the Norfloxacin monoclonal antibody and the sulfamethoxazole monoclonal antibody are obtained by taking Norfloxacin hapten, sulfamethoxazole hapten and carrier protein conjugate as immunogen; the quinolone compound is at least one of the following 13 types of compounds: Ciprofloxacin, Norfloxacin, Pefloxacin, Ofloxacin, Enoxacin, Marbofloxacin, Lomefloxacin, Danofloxacin, Enrofloxacin, Sarafloxacin, Difloxacin, Oxolinic acid and Flumequine; and the sulfonamide compound is at least one of the following 6 types of compounds: sulfapyridine, sulfathiazole, sulphapyridine, sulfamethizole, sulfamonomethoxine and sulfamethoxazole.

The invention discloses a method for determining 10 quinolone antibiotics in bean sprouts by an ultra-high performance liquid chromatography-tandem mass spectrometry, which is characterized by comprising the following steps of: preprocessing; preparing a standard solution; obtaining a liquid chromatography mass spectrogram and a regression equation; and analyzing and detecting samples by the ultra-high performance liquid chromatography-tandem mass spectrometry. A ultra-high performance liquid chromatography-electrospray tandem mass spectrometry is adopted in this study to carry out an analysisand determination of enrofloxacin, ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, sarafloxacin, danofloxacin, sparfloxacin, fleroxacin and lomefloxacin in the bean sprouts. Sample extraction conditions, purification means, liquid chromatography-mass spectrum parameters and the like are optimized and the matrix effect of the method is evaluated. The result shows that an establishment of the method solves the problem of a simultaneous determination of various quinolone antibiotics in the bean sprouts and provides a technical reference for risk assessment and government supervision.

The invention relates to a method for detecting a lomefloxacin enantiomer in an aquatic product. According to the present invention, the solid-phase extraction condition is optimized, such that the solid-phase extraction time is shortened, and the efficiency is high; the high performance liquid chromatography method for analyzing the lomefloxacin enantiomer concentration has characteristics of high precision, rapidness, simple operation, and low cost; and the detection method has characteristics of good linearity to the target compound, high recovery rate, low relative deviation, low toxicity of the used reagent, low consumption of the used reagent, and environmental protection.

A method for extracting and analyzing quinolone drugs by using a DPX tip-type dispersed solid-phase micro-extraction column relates to a method for detecting quinolone drug residues in animal-derivedfood. The invention aims to solve the problem that there is no effective method for detecting quinolone drugs in animal-derived food, especially measuring with quantitative high-precision, high-throughput and low-consumption in the prior art. According to the invention, a sample preparation and high performance liquid chromatography-tandem mass spectrometry method for detecting multi-residues of quinolones in animal-derived food is established. The method is suitable for the detection of single or multiple drug residues of enrofloxacin, ciprofloxacin, sarafloxacin, norfloxacin, ofloxacin, lomefloxacin and danofloxacin in animal-derived food.

The present invention relates to a crystal form of lomefloxacin aspartate and a preparation method thereof. The present invention also relates to a pharmaceutical composition containing the above-mentioned crystal form of lomefloxacin aspartate and the crystal form is used to manufacture a treatment for Gram Human or animal respiratory tract infection, genitourinary system infection, gastrointestinal bacterial infection, abdominal cavity, biliary tract, typhoid and other infections, bone and joint infection, skin and soft tissue infection, sepsis and other systemic infection caused by positive or negative bacterial sensitive bacteria, Other infections, such as sinusitis, otitis media, blepharitis and other drugs.

Methods and compositions are disclosed utilizing the optically pure (S)-isomer of lomefloxacin to treat bacterial infection. In particular, this compound is a potent drug for the treatment of Mycobacteria infection.

An anti-quinolone antibiotic class specific monoclonal antibody hybridoma cell strain YH6 and an application thereof belong to the technical field of immunochemistry. The monoclonal cell strain YH6 is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.12024. A monoclonal antibody secreted by the YH6 is detected by indirect competitive enzyme-linked immunosorbent assay, and has cross reaction with the following 21 pyrethroids: norfloxacin, ofloxacin, enrofloxacin, ciprofloxacin, flumequine, nafloxacin, enoxacin, lomefloxacin, levofloxacin, pefloxacin, nalidixic acid, danofloxacin, pyridine acid, cinoxacin, oxolinic acid, marbofloxacin, pazufloxacin, sparfloxacin, gatifloxacin, orbifloxacin and fleroxacin, and the IC50 value of the monoclonal antibody is 0.1-50 ng / mL. The class specific monoclonal antibody can be used for developing colloidal gold immunochromatographic test strips and immunosensors, provides a raw material for the immunodetection of quinolone antibiotic residues in foods, and has practical application values.

The invention discloses a lomefloxacin coupling compound with general formula (I), which comprises coupling compounds of lomefloxacin hapten and bovine serum albumin of carrier substance which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of lomefloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining lomefloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the lomefloxacin coupling compound of this invention has prepared antiserum with 1:6400 of potency, of which the lowest check limit is 1ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of lomefloxacin.

The invention relates to a lomefloxacin aspartate hydrate and the preparation, as well as the usage. The lomefloxacin aspartate hydrate of the invention (the molecular formula is C17H19F2N3O3.C4H7NO4.nH2O, n is equal to 0.5 to 2.5), the lomefloxacin aspartate hydrate of the invention is the fluoroquinolone antibacterial agent used for human or animal respiratory infection, genitourinary infection, gastrointestinal tract bacterial infection, abdominal infection, bile duct infection, typhoid and other infections, bone and joint infection, skin soft tissue infection, sepsis and other systemic infection, other infections, such as paranasal sinusitis, otitis media, blepharitis and so on which are caused by Gram positive or negative bacteria and sensitive bacteria, the invention has the advantages of stable existence, easy storage and transportation and convenient preparation of the pharmaceutical preparations.

The present invention features an antibacterial composition comprising 1) a composition A comprising polymyxin B and trimethoprim; and 2) an antibiotic agent selected from the group consisting of rifampicin, rifabutin, rifapentine, rifaximin, pefloxacin mesylate, sparfloxacin, sarafloxacin HCl, tobramycin, lomefloxacin, besifloxacin, danofloxacin mesylate, enrofloxacin, nadifloxacin and clinafloxacin, a topical pharmaceutical thereof, and a method of treating bacterial infections using mixtures of 1 and 2.

The invention discloses a lomefloxacin coupling compound with general formula (I), which comprises coupling compounds of lomefloxacin hapten and bovine serum albumin of carrier substance which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of lomefloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining lomefloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the lomefloxacin coupling compound of this invention has prepared antiserum with 1:6400 of potency, of which the lowest check limit is 1ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of lomefloxacin.

The invention discloses hybridoma WXX-1 capable of secreting an anti-lomefloxacin monoclonal antibody and an application thereof, and belongs to the technical field of immunochemistry. A murine monoclonal cell strain WXX-1 is prepared by a conventional cell fusion technique, and has reserved in China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.13088. The monoclonal antibody secreted by the cell strain is measured by using an indirect competitive-euzymelinked immunosorbent assay method; IC50 of the antibody to lomefloxacin, norfloxacin and enoxacin are 0.19microgram / L, 0.17microgram / L and 0.23microgram / L, respectively. Recovery ranges of lomefloxacin, norfloxacin and enoxacin in milk are 98.02-107.40%, 82.175%-105.55% and 92.47%-105.52%, respectively. The hybridoma provided by the invention provides materials for immunodetection of residual quinolone antibiotics in food, and has an actual application value.

A hydrated lomefloxacin aspartate (C17H19F2N3O3.C4H7NO4.nH2O, where n=0.5-2.5) is an antibacterial fluoroquinolone medicine for treating the various infections of the gram-positive and gram-negative bacteria, and its preparing process are disclosed.

The invention provides a lomefloxacin hydrochloride impurity and a preparation method thereof, 2, 3, 4-trifluoroaniline is used as a raw material to prepare trifluoroaniline lomefloxacin as the lomefloxacin hydrochloride impurity through ring formation, substitution, hydrolysis, acylation, condensation and other reactions, and an impurity reference substance is provided for external standard method quantitative detection of the trifluoroaniline lomefloxacin impurity in lomefloxacin hydrochloride. And the lomefloxacin hydrochloride impurity trifluoroaniline lomefloxacin synthesized by the method is high in yield and high in purity, and the detection accuracy of the impurity trifluoroaniline lomefloxacin in the bulk drug is improved.

The invention discloses bis-fluoroquinolone thiadiazole urea N-methyl lomefloxacin derivatives as well as a preparation method and an application thereof. The general chemical structure formula of thederivatives is shown in the formula I as follows in the description. In the formula I, R is ethyl, cyclopropyl, fluoroethyl, an oxazine ring formed with C-8 or a thiazine ring formed with C-8; L is an independent Cl or F atom, 1-piperazinyl, substituted piperazine-1-yl or a nitrogen fused heterocyclic ring; X is CH, N atom or F-substituted C atom (F-C) or a methoxy substituted C atom (CH3O-C). The bis-fluoroquinolone thiadiazole urea N-methyl lomefloxacin derivatives realize organic combination of a bis-fluoroquinolone skeleton and the thiadiazole heterocyclic ring with functional urea pharmacophores, so that transition and superposition of different pharmacophores are realized, anti-tumor activity and selectivity of fluoroquinolone are improved, toxic and side effects on normal cells arereduced, and the derivatives can be taken as an anti-tumor active substance for developing anti-tumor drugs with novel structures.

The invention relates to fructose injection of an antibiotic medicine. The fructose injection of the antibiotic medicine consists of antibiotics, fructose and water and also comprises proper additives, wherein the antibiotics comprise gatifloxacin, levofloxacin, ciprofloxacin, pazufloxacin, fleroxacin, sparfloxacin, moxifloxacin, pefloxacin, rufloxacin, lomefloxacin, norfloxacin, caderofloxacin, azithromycin, telithromycin, ornidazole, secnidazole, tinidazole, metronidazole, clindamycin, lincomycin, fluconazole, etimicin, netilmicin, amikacin as well as medicinal acid addition salt, esterification compounds, derivatives and the like; the fructose injection is prepared from the antibiotics; the advantages that the injection is convenient to use and takes effect rapidly are achieved; and compared with the glucose injection, the fructose injection is easier to absorb and utilize, more suitable for antisepsis and anti-inflammation, energy supply and body liquid supplementation of patientssuffering from diabetes, heart diseases and liver diseases, and enlarges the use range.

The invention discloses a magnetic reduced graphene oxide / silver tungstate composite photocatalyst as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, preparing graphene oxide by adopting an improved Hummers' method; 2, preparing magnetic reduced graphene oxide by adopting a coprecipitation method; 3, preparing magnetic reduced graphene oxide / silver tungstate by adopting a hydrothermal method. The magnetic reduced graphene oxide / silver tungstate composite photocatalyst disclosed by the invention can be used for effectively adsorbing and degrading residual lomefloxacin antibiotics in water. The material has relatively strong adsorption effect and photodegradation effect, can be magnetically recycled, avoids secondary pollution and has relatively good biocompatibility.

The invention relates to a lomefloxacin aspartate hydrate and its novel preparation method and use. The novel preparation method of the lomefloxacin aspartate hydrate comprises the following steps of dissolving lomefloxacin aspartate in one or more of water, C3-C6 low-grade ketones and C1-C6 low-molecular weight alcohols as solvents in a reactor, adjusting a pH value to 6-8.5 by an alkali, adding L, D or DL aspartic acid or its solution into the reactor, stirring for dissolution so that a reaction is finished, slowly adding one or more of C3-C8 low-grade ketones, C1-C6 low-molecular weight alcohols and C2-C6 low-grade ethers into the reactor, cooling to a temperature of -20 to 20 DEG C, filtering after solids are precipitated, carrying out rinsing by one or more of organic solvents of C1-C6 low-molecular weight alcohols, C3-C8 low-grade ketones, C2-C6 low-grade ethers and C1-C6 low-grade halohydrocarbons, carrying out vacuum-pumping and drying to obtain the lomefloxacin aspartate hydrate. The lomefloxacin aspartate hydrate is powder of which the color changes form almost white to faint yellow and has good storage stability.

The invention provides a fructose injection of an antibiotic drug. The injection is composed of antibiotics, fructose and water. The injection also can contain proper additives. The antibiotic comprise gatifloxacin, levofloxacin, ofloxacin, ciprofloxacin, pazufloxacin, fleroxacin, sparfloxacin, moxifloxacin, pefloxacin, rufloxacin, lomefloxacin, norfloxacin, caderofloxacin, azithromycin, telithromycin, ornidazole, secnidazole, tinidazole, metronidazole, clindamycin, lincomycin, fluconazole, etimicin, netilmicin, amikacin and medicinal acid additive salts, esterified compounds, derivatives and the like. The antibiotics are prepared into the fructose injection. Besides the advantages of convenience in use and quickness to take effect of the injection, compared with a gluconic infection, the injection provided by the invention is more easily absorbed and utilized, is more suitable for preventing bacteria and diminishing inflammation, supplying energy and supplementing body fluids for patients with diabetes, heart disease and liver disease, so that the application range of the injection is expanded.

The invention provides an isocryptolepine analogue, a preparation method of the isocryptolepine analogue from lomefloxacin and an application thereof, which are used for solving the technical problem of how to design an indoloquinoline antituberculosis drug with a novel structure by taking cryptolepine alkaloid as a primer and using an atom economic strategy. According to the invention, the isocryptolepine analogue is prepared from lomefloxacin, so that effective chemical construction from a fluoroquinolone structure to an indoloquinoline skeleton is realized, a new way for structural modification of iso-cryptolepine is expanded, and complementation of dominant structures of fluoroquinolone drugs and natural indoloquinoline alkaloids is achieved. An in-vitro anti-tuberculosis activity test result shows that the compound has relatively good growth inhibition activity on a tested tubercle bacillus strain, the activity of parts of the compound is equivalent to that of a control isoniazide, and the compound has drug resistance and relatively low cytotoxicity, and can be further developed and prepared as an anti-tuberculosis drug with a brand new structure.

The invention provides a lomefloxacin immunogen which has the structure represented by the formula I. A preparation method of the lomefloxacin immunogen is provided and comprises the steps of carrying out C chain derivatization of carboxyl on lomefloxacin, and activating BSA by using a glycosylation modification method, then synthesizing and immunizing by using a carbodiimide two-step method, and thus obtaining the immunogen having the characteristics of high coupling rate and strong immunity. The invention also provides a test paper card for detecting the lomefloxacin, wherein the test paper card has the advantages of fast detection speed and high sensitivity; and the detection limit of the test paper card can be reach 0.3 ng / ml, and the requirement of rapid detection can be met.

The invention relates to a preparation method of an anti-infectious agent, particularly relates to a preparation method of lomefloxacin aspartate. The preparation method comprises the steps of performing a neutralization reaction on lomefloxacin hydrochloride, water and an alkaline solution in proportion, obtaining lomefloxacin base, performing a salt forming reaction on the lomefloxacin base generated by the neutralization reaction, L-aspartate and the water in proportion, obtaining a lomefloxacin aspartate wet product, refining the lomefloxacin base, the L-aspartate and mother liquor obtained by a purified water salt forming reaction, obtaining the lomefloxacin aspartate wet product, and finally drying the lomefloxacin aspartate wet product, so as to obtain a finished product, generally, the preparation method has the advantages that the lomefloxacin aspartate can react in the water, the production cost can be effectively reduced, the reaction speed can be improved, the safety in the production process can be improved, the economic benefit of enterprise can be improved, and a high use value can be realized.

An anti-quinolone antibiotic class specific monoclonal antibody hybridoma cell strain YH6 and an application thereof belong to the technical field of immunochemistry. The monoclonal cell strain YH6 is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.12024. A monoclonal antibody secreted by the YH6 is detected by indirect competitive enzyme-linked immunosorbent assay, and has cross reaction with the following 21 pyrethroids: norfloxacin, ofloxacin, enrofloxacin, ciprofloxacin, flumequine, nafloxacin, enoxacin, lomefloxacin, levofloxacin, pefloxacin, nalidixic acid, danofloxacin, pyridine acid, cinoxacin, oxolinic acid, marbofloxacin, pazufloxacin, sparfloxacin, gatifloxacin, orbifloxacin and fleroxacin, and the IC50 value of the monoclonal antibody is 0.1-50 ng / mL. The class specific monoclonal antibody can be used for developing colloidal gold immunochromatographic test strips and immunosensors, provides a raw material for the immunodetection of quinolone antibiotic residues in foods, and has practical application values.

The invention provides a detection method of four forbidden fluoroquinolone drug residues in aquaculture water, and belongs to the technical field of environment quality security detection. The methodcomprises the following steps: taking 50mL of a water sample, adding an acetic acid-acetonitrile solution with the same volume to extract, and shaking out for 30min; adding an appropriate amount of sodium chloride, mixing for 1min, and standing for layering; taking 25mL of supernate, and concentrating, namely blowing the supernate with nitrogen at 40 DEG C till to nearly dry; adding 1mL of 0.1% formic acid-water-methanol (90+10) to re-dissolve, filtering through a filter membrane of 0.22 micron for future detection. The detection method disclosed by the invention has the advantages that the pretreatment for detecting the residues of ofloxacin, pefloxacin, lomefloxacin and norfloxacin in the aquaculture water is simple and convenient, the liquid chromatogram-tandem mass spectrometry and multiple reaction monitoring modes are adopted, the determination on the nature and the quantitation is accurate, the sensitivity is high, the reproducibility is good. Theoretical support of the detection method is provided for formulating related industry standard by the nation.

The invention discloses a composition preparation of a lomefloxacin compound and sodium chloride. The composition preparation is injection, wherein the injection is prepared from lomefloxacin, sodium chloride, glutamic acid, disodium edentate, citric acid and water for injection, and each 100 milliliters of injection contains 200 milligrams of lomefloxacin, 900 milligrams of sodium chloride, 5 to20 milligrams of glutamic acid, 2 to 5 milligrams of disodium edentate, 10 to 30 milligrams of citric acid and the balance of water for injection. The composition preparation of the lomefloxacin compound and the sodium chloride has high stability. The invention also provides a preparation method for the composition preparation of the lomefloxacin compound and the sodium chloride.

The present invention relates to a lomefloxacin eye gel preparation. Said preparation is formed from lomefloxacin and pharmaceutically-acceptable carrier suitable for making eye gel. Its formula composition includes carbomer 941, NaOH, NaCl and methylparaben.