44 results about "Lomefloxacin" patented technology

The invention relates to a method for splitting a chiral medicine of hydrochloric acid lomefloxacin, adopting a high-speed counter-current chromatography method to separate out levorotatory hydrochloric acid lomefloxacin and dextrorotatory hydrochloric acid lomefloxacin from a hydrochloric acid lomefloxacin crude extract. A solvent system comprises three components of ethers or normal paraffin hydrocarbon or fatty ester, acetonitrile or fatty alcohol or fatty ketone and water, the volume ratio is sequentially 5-100:1:5-100, an upper phase is used as a stationary phase, a lower phase is used as a flowing phase, sulphonation-beta-schardinger dextrin is used as a chiral splitting reagent, the content of the sulphonation-beta-schardinger dextrin in the lower phase is 0.02-0.06mol/L, and a chiral flowing phase method is adopted to split a hydrochloric acid lomefloxacin enantiomorph. The method for splitting the chiral medicine of the hydrochloric acid lomefloxacin can achieve higher separating degree, is suitable for various types of prepared type counter-current chromatography instruments and can separate and obtain the monomers of the levorotatory hydrochloric acid lomefloxacin and the dextrorotatory hydrochloric acid lomefloxacin.

The invention belongs to the technical field of molecularly imprinted polymers, and particularly discloses a magnetic carboxylation nano-crystalline cellulose amino-functionalization surface molecularly imprinted polymer. Magnetic carboxylation nano-crystalline cellulose Fe3O4@CCNs serves as a carrier, ofloxacin OFX serves as template molecules, glycidyl methacrylate GMA serves as a functional monomer, and through a polymerization reaction, the magnetic carboxylation nano-crystalline cellulose amino-functionalization surface molecularly imprinted polymer Fe3O4@CCNs@MIPs is formed. Fluoroquinolone drugs in a water body can be selectively extracted, and include ofloxacin OFX, lomefloxacin LOM, gatifloxacin GAT, sarafloxacin SARA, marbofloxacin MARBO, orbifloxacin OBX, difloxacin DFX, ciprofloxacin CIP, enrofloxacin ENRO, sparfloxacin SPX, and moxifloxacin MFX, the recovery rate reaches 81.2%-93.7%, and the adsorption capacity reaches 33 mg/g after adsorption/elution are repeated seven times.

The invention discloses a method and special immune affinity adsorbent for extracting quinolone compound and/or sulfonamide compound. The immune affinity adsorbent consists of a solid-phase carrier and Norfloxacin monoclonal antibody and/or sulfamethoxazole monoclonal antibody coupled with the carrier, wherein the Norfloxacin monoclonal antibody and the sulfamethoxazole monoclonal antibody are obtained by taking Norfloxacin hapten, sulfamethoxazole hapten and carrier protein conjugate as immunogen; the quinolone compound is at least one of the following 13 types of compounds: Ciprofloxacin, Norfloxacin, Pefloxacin, Ofloxacin, Enoxacin, Marbofloxacin, Lomefloxacin, Danofloxacin, Enrofloxacin, Sarafloxacin, Difloxacin, Oxolinic acid and Flumequine; and the sulfonamide compound is at least one of the following 6 types of compounds: sulfapyridine, sulfathiazole, sulphapyridine, sulfamethizole, sulfamonomethoxine and sulfamethoxazole.

The invention discloses a method for determining 10 quinolone antibiotics in bean sprouts by an ultra-high performance liquid chromatography-tandem mass spectrometry, which is characterized by comprising the following steps of: preprocessing; preparing a standard solution; obtaining a liquid chromatography mass spectrogram and a regression equation; and analyzing and detecting samples by the ultra-high performance liquid chromatography-tandem mass spectrometry. A ultra-high performance liquid chromatography-electrospray tandem mass spectrometry is adopted in this study to carry out an analysisand determination of enrofloxacin, ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, sarafloxacin, danofloxacin, sparfloxacin, fleroxacin and lomefloxacin in the bean sprouts. Sample extraction conditions, purification means, liquid chromatography-mass spectrum parameters and the like are optimized and the matrix effect of the method is evaluated. The result shows that an establishment of the method solves the problem of a simultaneous determination of various quinolone antibiotics in the bean sprouts and provides a technical reference for risk assessment and government supervision.

The invention relates to a method for detecting a lomefloxacin enantiomer in an aquatic product. According to the present invention, the solid-phase extraction condition is optimized, such that the solid-phase extraction time is shortened, and the efficiency is high; the high performance liquid chromatography method for analyzing the lomefloxacin enantiomer concentration has characteristics of high precision, rapidness, simple operation, and low cost; and the detection method has characteristics of good linearity to the target compound, high recovery rate, low relative deviation, low toxicity of the used reagent, low consumption of the used reagent, and environmental protection.

The present invention relates to a crystal form of lomefloxacin aspartate and a preparation method thereof. The present invention also relates to a pharmaceutical composition containing the above-mentioned crystal form of lomefloxacin aspartate and the crystal form is used to manufacture a treatment for Gram Human or animal respiratory tract infection, genitourinary system infection, gastrointestinal bacterial infection, abdominal cavity, biliary tract, typhoid and other infections, bone and joint infection, skin and soft tissue infection, sepsis and other systemic infection caused by positive or negative bacterial sensitive bacteria, Other infections, such as sinusitis, otitis media, blepharitis and other drugs.

Methods and compositions are disclosed utilizing the optically pure (S)-isomer of lomefloxacin to treat bacterial infection. In particular, this compound is a potent drug for the treatment of Mycobacteria infection.

The present invention features an antibacterial composition comprising 1) a composition A comprising polymyxin B and trimethoprim; and 2) an antibiotic agent selected from the group consisting of rifampicin, rifabutin, rifapentine, rifaximin, pefloxacin mesylate, sparfloxacin, sarafloxacin HCl, tobramycin, lomefloxacin, besifloxacin, danofloxacin mesylate, enrofloxacin, nadifloxacin and clinafloxacin, a topical pharmaceutical thereof, and a method of treating bacterial infections using mixtures of 1 and 2.

The invention discloses hybridoma WXX-1 capable of secreting an anti-lomefloxacin monoclonal antibody and an application thereof, and belongs to the technical field of immunochemistry. A murine monoclonal cell strain WXX-1 is prepared by a conventional cell fusion technique, and has reserved in China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.13088. The monoclonal antibody secreted by the cell strain is measured by using an indirect competitive-euzymelinked immunosorbent assay method; IC50 of the antibody to lomefloxacin, norfloxacin and enoxacin are 0.19microgram/L, 0.17microgram/L and 0.23microgram/L, respectively. Recovery ranges of lomefloxacin, norfloxacin and enoxacin in milk are 98.02-107.40%, 82.175%-105.55% and 92.47%-105.52%, respectively. The hybridoma provided by the invention provides materials for immunodetection of residual quinolone antibiotics in food, and has an actual application value.

The invention provides a lomefloxacin hydrochloride impurity and a preparation method thereof, 2, 3, 4-trifluoroaniline is used as a raw material to prepare trifluoroaniline lomefloxacin as the lomefloxacin hydrochloride impurity through ring formation, substitution, hydrolysis, acylation, condensation and other reactions, and an impurity reference substance is provided for external standard method quantitative detection of the trifluoroaniline lomefloxacin impurity in lomefloxacin hydrochloride. And the lomefloxacin hydrochloride impurity trifluoroaniline lomefloxacin synthesized by the method is high in yield and high in purity, and the detection accuracy of the impurity trifluoroaniline lomefloxacin in the bulk drug is improved.

The invention discloses bis-fluoroquinolone thiadiazole urea N-methyl lomefloxacin derivatives as well as a preparation method and an application thereof. The general chemical structure formula of thederivatives is shown in the formula I as follows in the description. In the formula I, R is ethyl, cyclopropyl, fluoroethyl, an oxazine ring formed with C-8 or a thiazine ring formed with C-8; L is an independent Cl or F atom, 1-piperazinyl, substituted piperazine-1-yl or a nitrogen fused heterocyclic ring; X is CH, N atom or F-substituted C atom (F-C) or a methoxy substituted C atom (CH3O-C). The bis-fluoroquinolone thiadiazole urea N-methyl lomefloxacin derivatives realize organic combination of a bis-fluoroquinolone skeleton and the thiadiazole heterocyclic ring with functional urea pharmacophores, so that transition and superposition of different pharmacophores are realized, anti-tumor activity and selectivity of fluoroquinolone are improved, toxic and side effects on normal cells arereduced, and the derivatives can be taken as an anti-tumor active substance for developing anti-tumor drugs with novel structures.

The invention discloses a magnetic reduced graphene oxide/silver tungstate composite photocatalyst as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, preparing graphene oxide by adopting an improved Hummers' method; 2, preparing magnetic reduced graphene oxide by adopting a coprecipitation method; 3, preparing magnetic reduced graphene oxide/silver tungstate by adopting a hydrothermal method. The magnetic reduced graphene oxide/silver tungstate composite photocatalyst disclosed by the invention can be used for effectively adsorbing and degrading residual lomefloxacin antibiotics in water. The material has relatively strong adsorption effect and photodegradation effect, can be magnetically recycled, avoids secondary pollution and has relatively good biocompatibility.

The invention provides a fructose injection of an antibiotic drug. The injection is composed of antibiotics, fructose and water. The injection also can contain proper additives. The antibiotic comprise gatifloxacin, levofloxacin, ofloxacin, ciprofloxacin, pazufloxacin, fleroxacin, sparfloxacin, moxifloxacin, pefloxacin, rufloxacin, lomefloxacin, norfloxacin, caderofloxacin, azithromycin, telithromycin, ornidazole, secnidazole, tinidazole, metronidazole, clindamycin, lincomycin, fluconazole, etimicin, netilmicin, amikacin and medicinal acid additive salts, esterified compounds, derivatives and the like. The antibiotics are prepared into the fructose injection. Besides the advantages of convenience in use and quickness to take effect of the injection, compared with a gluconic infection, the injection provided by the invention is more easily absorbed and utilized, is more suitable for preventing bacteria and diminishing inflammation, supplying energy and supplementing body fluids for patients with diabetes, heart disease and liver disease, so that the application range of the injection is expanded.

The invention provides an isocryptolepine analogue, a preparation method of the isocryptolepine analogue from lomefloxacin and an application thereof, which are used for solving the technical problem of how to design an indoloquinoline antituberculosis drug with a novel structure by taking cryptolepine alkaloid as a primer and using an atom economic strategy. According to the invention, the isocryptolepine analogue is prepared from lomefloxacin, so that effective chemical construction from a fluoroquinolone structure to an indoloquinoline skeleton is realized, a new way for structural modification of iso-cryptolepine is expanded, and complementation of dominant structures of fluoroquinolone drugs and natural indoloquinoline alkaloids is achieved. An in-vitro anti-tuberculosis activity test result shows that the compound has relatively good growth inhibition activity on a tested tubercle bacillus strain, the activity of parts of the compound is equivalent to that of a control isoniazide, and the compound has drug resistance and relatively low cytotoxicity, and can be further developed and prepared as an anti-tuberculosis drug with a brand new structure.

The invention relates to a preparation method of an anti-infectious agent, particularly relates to a preparation method of lomefloxacin aspartate. The preparation method comprises the steps of performing a neutralization reaction on lomefloxacin hydrochloride, water and an alkaline solution in proportion, obtaining lomefloxacin base, performing a salt forming reaction on the lomefloxacin base generated by the neutralization reaction, L-aspartate and the water in proportion, obtaining a lomefloxacin aspartate wet product, refining the lomefloxacin base, the L-aspartate and mother liquor obtained by a purified water salt forming reaction, obtaining the lomefloxacin aspartate wet product, and finally drying the lomefloxacin aspartate wet product, so as to obtain a finished product, generally, the preparation method has the advantages that the lomefloxacin aspartate can react in the water, the production cost can be effectively reduced, the reaction speed can be improved, the safety in the production process can be improved, the economic benefit of enterprise can be improved, and a high use value can be realized.

The invention provides a detection method of four forbidden fluoroquinolone drug residues in aquaculture water, and belongs to the technical field of environment quality security detection. The methodcomprises the following steps: taking 50mL of a water sample, adding an acetic acid-acetonitrile solution with the same volume to extract, and shaking out for 30min; adding an appropriate amount of sodium chloride, mixing for 1min, and standing for layering; taking 25mL of supernate, and concentrating, namely blowing the supernate with nitrogen at 40 DEG C till to nearly dry; adding 1mL of 0.1% formic acid-water-methanol (90+10) to re-dissolve, filtering through a filter membrane of 0.22 micron for future detection. The detection method disclosed by the invention has the advantages that the pretreatment for detecting the residues of ofloxacin, pefloxacin, lomefloxacin and norfloxacin in the aquaculture water is simple and convenient, the liquid chromatogram-tandem mass spectrometry and multiple reaction monitoring modes are adopted, the determination on the nature and the quantitation is accurate, the sensitivity is high, the reproducibility is good. Theoretical support of the detection method is provided for formulating related industry standard by the nation.