70 results about "Teicoplanin" patented technology

The invention provides a method for making high-purity teicoplanin. The method comprises the following stages that: stage a, gel chromatography and macropore fractionation by adsorption of a teicoplanin crude product solution are carried out so as to obtain a teicoplanin crude product solution; stage b, active carbon decoloring, ultrafiltration, nanofiltration and crystallization of the teicoplanin crude product solution are carried out so as to obtain high-purity teicoplanin. The method furthest increases the production efficiency of teicoplanin, thereby realizing the industrialization of high-purity teicoplanin.

Disclosed is a method of producing teicoplanin. The method includes purifying teicoplanin from a culture broth, obtained by culturing microorganisms capable of producing teicoplanin by a porous adsorption resin under selective elution conditions and recovering highly pure teicoplanin using activated carbon and/or ultrafiltration. In this regard, the method can further include ultrafiltration as pre-treatment before the culture broth is adsorbed into the porous adsorption resin so as to increase the purity of teicoplanin.

The invention relates to a preparation method of single teicoplanin components, which belongs to the field of preparation of medicaments with microbial ingredients. The method comprises the following steps of: decolorizing teicoplanin fermentation liquor through macroporous decolorizing resin, and absorbing the teicoplanin fermentation liquor through macroporous adsorption resin, then desorbing the teicoplanin fermentation liquor, and concentrating the teicoplanin fermentation liquor to obtain a crude teicoplanin extract including the six components of TA2-1, TA2-2, TA2-3, TA2-4, TA2-5 and TA3-1; and dissolving the crude extract in water, and performing chromatographic separation on the dissolved crude extract through polymer microspheres to obtain the six single components, namely TA2-1, TA2-2, TA2-3, TA2-4, TA2-5 and TA3-1, of teicoplanin with high purity. According to the separation method of the single teicoplanin components, the novel chromatography media-polymer microspheres are adopted to simultaneously separate the six single components from the crude teicoplanin extract; and the method is simple in technology, can be used for disposing a large quantity of samples, consumes less solvent, can be used for preparing the single teicoplanin components in a large scale, and provides samples for intensive study on pathology, toxicology and the like of the single teicoplanin components.

The invention relates to a carbanilate group carbanilate group chirality fixed phase stuff and relative production, wherein it uses silica gel as carrier; the carrier and 3-propyl triethoxysilicane to process silanization reaction; activates the carrier and distant arm; bonds silica carrier and peptide macrocyclic antibiotics, processes derivation with carbanilate group. The inventive fixed phase stuff has high chirality recognize ability and high stability to be used in any spectrum conditions. The invention can separate glutacid in reverse flow phase, to separate the salbutamolum in polar flow phase.

The invention discloses a separating and purifying method for obtaining highly pure teicoplanin. The method comprises the following steps: removing solids in a teicoplanin fermenting liquid through using a microfiltration membrane, and concentrating an obtained teicoplanin-containing micro-filtrate through a nanofiltration membrane to obtain a teicoplanin concentrate; carrying out adsorption elution of the concentrate through a macroporous adsorption resin twice to obtain a highly pure teicoplanin eluate; carrying out nanofiltration concentration of the eluate, adding a protein precipitating agent acetone to the concentrate for precipitating proteins of the concentrate, and filtering for removing the proteins to obtain a filtrate; adding active carbon to the filtrate for decoloring, and filtering to obtain a decolored liquid; and adding acetone to the decolored liquid, carrying out low temperature crystallization, and carrying out vacuum drying of obtained crystals to obtain finally obtained highly pure teicoplanin having a purity of above 96%. The method has the advantages of low cost, high separation purity of teicoplanin, and suitableness for the commercial production.

The invention provides a supplemented culture medium of teicoplanin, which is capable of improving the output of teicoplanin and a method for producing the teicoplanin. The supplemented culture medium of the teicoplanin comprises one or more of a carbon source, a nitrogen source and a pH value regulator, wherein the carbon source comprises one or more of glycerol, glucose, starch, oat flour and lactose; the nitrogen source comprises one or more of corn gluten meal, cottonseed fine lour, soybean meal, peptone and yeast powder; and the pH value regulator comprises one or two of phosphoric acid and ammonia water. The method for producing the teicoplanin specifically comprises a step of adding the supplemented culture medium into a fermentation culture medium during fermentation, wherein the supplemented culture medium comprises one or more of the carbon source, the nitrogen source and the pH value regulator. The invention has the beneficial effects that the supplemented culture medium is added into the fermentation culture medium during fermentation to reduce factors inhibiting secondary metabolic pathways for producing the teicoplanin, and the fermentation output of the teicoplanin can be improved.

The invention relates to a method for separating and measuring content of Palonosetron hydrochloride and optical isomers thereof by high performance liquid chromatography, which is characterized in that: a chromatographic column packing material used in the high performance liquid chromatography is macrocyclic glycopeptides and teicoplanin bonded silica gel, and a mobile phase consists of aqueoussolution of triethylamine and organic solvent.

The invention describes an antibiotic coating for porous bodies and its use. Into the porous system of non-metallic porous bodies and of metallic porous bodies, a coating made of at least one antibiotic salt that is hardly soluble in water or in an aqueous environment from the group of the netilmicin laurate, the netilmicin myristate, the netilmicin dodecyl sulfate, the sisomicin laurate, the sisomicin myristate, the sisomicin dodecyl sulfate, the gentamicin laurate, the gentamicin myristate, the clindamycin laurate, the amikacin laurate, the amikacin myristate, the amikacin dodecyl sulfate, the kanamycin laurate, the kanamycin myristate, the kanamycin dodecyl sulfate, the tobramycin laurate, the tobramycin myristate, the tobramycin dodecyl sulfate, the ciprofloxacin myristate, the vancomycin dodecyl sulfate, the vancomycin laurate, the vancomycin myristate, the vancomycin teicoplanin and the clindamycin teicoplanin is introduced. The antibiotically coated, porous bodies are used as implants.

The invention provides a purification method of high-purity teicoplanin refined powder, which comprises the following steps: centrifuging a teicoplanin fermentation liquid under alkaline conditions to remove solids in the fermentation liquid, regulating the filtrate to a neutral state, adsorbing and eluting with a macroporous adsorbent resin to obtain a teicoplanin eluate with the concentration of 65% above, and filtering by gel chromatography to obtain a 80% teicoplanin eluate; concentrating the eluate, adding acetone to precipitate, and centrifuging to obtain a milk white teicoplanin crude product; and dissolving the crude product with a right amount of water, adding the sample to a chromatographic column filled with reversed phase silica gel, carrying out gradient elution with an acetonitrile-water mixed solution to obtain a high-purity teicoplanin eluate, adding activated carbon for decolorization, filtering, cooling to precipitate, removing the residual impurities, adding acetone to the clear solution to completely precipitate, and carrying out infrared drying on the wet product to obtain the teicoplanin pure product. The method has the advantages of simple technique, reliable quality, high purity of the teicoplanin refined powder and controllable single-component content, and is suitable for industrial large-scale production of high-purity teicoplanin.

The invention provides a method for separating and purifying teicoplanin. The method specifically comprises the following steps: filtering a teicoplanin fermentation liquid by means of a ceramic membrane; performing macroporous adsorption resin column chromatography; performing cation exchange resin column adsorption, and performing elution; performing nanofiltration on the eluate, and performing concentration; performing reverse chromatographic resin column adsorption, and performing elution; performing activated carbon decolorization; performing nanofiltration on the decolorization liquid, and performing concentration; performing ultrafiltration, performing nanofiltration, and performing concentration; and performing freeze-drying. The method overcomes the defects of the existing teicoplanin extraction technology, and improves the purity of the teicoplanin.

The invention relates to a medicinal composition for treating complex infection and a preparation method thereof. The medicinal composition is characterized by comprising an active ingredient selected from one of glycopeptides antibacterial drugs of telavancin, dalbavancin, oritavancin, vancomycin, teicoplanin or a pharmaceutically acceptable salt thereof, and also comprising polyene and echinocandin type antifungal agents, and being prepared into a freeze-dried powder injection by adopting a conventional process. The medicinal composition provided by the invention realizes combined application of the antibacterial drugs and the antifungal agents, can simultaneously give play to an antibacterial infection effect and an antifungal infection effect, and is convenient for clinical use.

The invention provides application of teicoplanin to resisting Ebola virus. The teicoplanin can inhibit the envelope protein GP of Ebola virus. The envelope protein GP is the Zaire envelope protein of Ebola virus breaking out in 2014. The teicoplanin can inhibit Ebola virus from entering host cells and acting in host cells.

The invention discloses a specific primer combination and a probe combination for detecting four glycopeptide drug resistance genes vanA, vanB, vanD and vanM in enterococcus bacteria, comprising a primer combination for specific amplification of four glycopeptide drug resistance genes and further comprising specific probes for detecting the four glycopeptide drug resistance genes. A method for detecting the four glycopeptide drug resistance genes by using the specific primer and probe combinations is further provided. The combinations have high specificity and coverage, can be used not only for detecting the drug resistance genes vanA, vanB, vanD and vanM in human infected samples but also for suggesting resistance degree to vancomycin and teicoplanin, and provides a reference basis for clinical anti-infective therapy medication. At the same time, the development of glycopeptides drug resistance is monitored. The combinations can also be used for monitoring the distribution and transfer of the four glycopeptide drug resistance genes in animal and environmental samples.

The invention provides an isotonic composition used for improving the stability of teicoplanin for injection and a preparation method of the isotonic composition. The isotonic composition comprises 0.1 g of the teicoplanin, 0.2 g of sodium chloride and/or glucose and 0.4 g of water for injection, wherein the pH value of the composition is 7.0-8.0. The isotonic composition used for improving the stability of the teicoplanin for injection can effectively improve the clarity, the stability is equivalent to that of a teicoplanin composition for injection only containing sodium chloride, and the isotonic composition can be stored at room temperature.

The invention discloses a preparation method, a product and an application of teicoplanin-functionalized gold nanoparticles. The preparation method comprises the following steps: mixing teicoplanin, chloroauric acid and water into a solution, adjusting the pH value of the solution with alkali till the pH value of the solution is in a range of 10-14 and the solution is brown, performing a stirring reaction at the temperature of 20-25 DEG C for 0.5-24 h till the solution becomes dark red, and performing dialysis to remove unreacted teicoplanin or chloroauric acid to obtain the teicoplanin-functionalized gold nanoparticles. According to the method, teicoplanin and chloroauric acid are subjected to a one-pot mixing reaction. The teicoplanin molecular structure contains hydroxyl and amino, which can be directly used as a stabilizer and reductant in the reaction. The method is quite simple and easy to operate. The teicoplanin-functionalized gold nanoparticles has better gram positive bacteria resistance performance than teicoplanin, and can be applied to the field of biological medicine.

The invention relates to a culture medium for producing teicoplanin by using actinoplanes and a culture method. The culture medium comprises a seed culture medium and a fermentation culture medium. Byusing a culture medium recipe and the culture method provided by the invention, the teicoplanin fermentation unit can be improved; the fermentation period can be shortened; the fermentation cost canbe reduced; the environment influence on raw and auxiliary material sources is reduced to the maximum degree; the sufficient supply of raw and auxiliary materials is ensured; and the stable and efficient production of the teicoplanin is realized.

The invention discloses a teicoplanin purifying method. The method comprises the following steps: cooling a teicoplanin fermentation liquid to 5-10 DEG C, adjusting the pH value of the teicoplanin fermentation liquid to 11-12, and carrying out ceramic membrane filtration to obtain a ceramic membrane filtrate; adjusting the pH value of the ceramic membrane filtrate by using hydrochloric acid, carrying out first stage resin column adsorption on the adjusted filtrate, and collecting obtained eluate; adding purified water to dilute the eluate, carrying out second stage resin column adsorption on the diluted eluate, eluting the diluted eluate with purified water, eluting the diluted eluate with methanol, and collecting obtained eluate; adding purified water to dilute the eluate, and concentrating the eluate by using a nanofiltration membrane; and carrying out active carbon decolorizing, crystallizing and drying: decolorizing the obtained nanofiltration concentrate by using active carbon, adding acetone used as a crystallization solvent, crystallizing the decolorized concentrate, and drying obtained crystals to obtain fine teicoplanin powder with the teicoplanin purity of 95% or above and the total yield of 60% or above. The purifying method has he advantages of high purity, high recovery rate and safety.