70 results about "Teicoplanin" patented technology

An isolated strain of Enterococcus faecalis GALT deposited under number CECT 7121 of the group of lactic bacteria is disclosed, which is capable of surviving and colonizing the gastrointestinal tract of humans and / or animals and showing beneficial probiotic activity for the health of humans and animals. The strain E. faecalis GALT and / or a culture supernatant and / or metabolites thereof shows no in vitro multiresistance to antibiotics of common use in human clinics as glycopeptides, such as vancomycin, teicoplanine; carbapenemes, such as impipenem, meropenem; and ampicillin. The strain E. faecalis GALT contains no red blood cell-destroying hemolysins of human, ovine and equine origin; and it does not produce any gelatinase, DNase and decarboxylases.The strain E. faecalis GALT is useful for the preparation of a composition intended for the treatment and / or prophylaxis of disorders associated with colonization by pathogenic microorganisms of the gastrointestinal tract; for use as a regulator of the immune response in human and animals, as well as for the preparation of a composition.The invention is also directed to methods and uses of the strain E. faecalis GALT.

The invention provides a purification method of teicoplanin, and the purification method comprises the following steps: (1) adjusting teicoplanin fermentation liquid to be alkaline, then performing filtration; (2) adjusting the filtrate obtained in step (1) to be acidic, eluting the filtrate through macroporous adsorption resin by using water and ammonia water in order, collecting the ammonia water eluate; (3) filtering the eluate obtained in step (2), separating the filtrate through silica gel chromatography, collecting the eluate of ethanol aqueous solution with a volume ratio of 10-50%; (4) adjusting the eluate obtained in step (3) to be acidic, performing ultrafiltration by an ultrafilter membrane with a cutoff molecular weight of 5000-30000 Da, preferably 10000 Da, so as to obtain an ultrafiltrate; and (5) performing nanofiltration of the ultrafiltrate obtained in step (4) through a nanofiltration membrane with a cutoff molecular weight of 100-1000 Da, preferably 200 Da, and concentrating to obtain the teicoplanin. The purification method of the invention improves the production efficiency of teicoplanin to the maximum extent, and thus realizes industrial production of high-purity teicoplanin.