Preparation method, product and application of teicoplanin-functionalized gold nanoparticles

A teicoplanin and functionalized technology, which is applied in the field of preparation of teicoplanin functionalized nano-gold, can solve the problems of time-consuming, complicated and the like, and achieve the effect of good antibacterial effect.

Active Publication Date: 2016-11-30
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, the preparation of antibiotic-gold nanoparticle complexes is mai

Method used

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  • Preparation method, product and application of teicoplanin-functionalized gold nanoparticles
  • Preparation method, product and application of teicoplanin-functionalized gold nanoparticles
  • Preparation method, product and application of teicoplanin-functionalized gold nanoparticles

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Experimental program
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Embodiment 1

[0026] Embodiment 1 Preparation of Teicoplanin Functionalized Nano Gold

[0027] Auric acid chloride (HAuCl) with a mass concentration of 1% 4 ) into the round bottom flask, and then add the teicoplanin whose mass concentration is 1%, wherein HAuCl 4 The molar ratio to teicoplanin is 4:1, then add a certain amount of water, stir at 20-25°C for 15 minutes, the solution is light yellow, then adjust the pH of the solution to 12 with NaOH, the solution is brown, 20-25 Stirring was continued for 30 min at ℃, the solution was wine red, and the solution was purified by dialysis using a dialysis bag with a regenerated fiber membrane and ultrapure water as a buffer to obtain Teicoplanin-AuNPs.

[0028] The teicoplanin-nanogold prepared in embodiment 1 is carried out ultraviolet absorption spectrum detection, obtains such as figure 1 As shown in the spectrogram, the maximum absorption wavelength of teicoplanin-nanogold is around 520nm, figure 2 Actual picture showing teicoplanin-gol...

Embodiment 2

[0032] Example 2 Antibacterial Activity Verification of Teicoplanin-Nanogold

[0033] will be 10 3 CFU / mL Staphylococcus aureus were mixed with phosphate buffered saline (PBS, 0.01M, pH7.4), teicoplanin-nanogold (0.2mg / mL), trisodium citrate modified gold nanoparticles (citrate-AuNPs ) solution (0.2mg / mL) and Teicoplanin (0.2mg / mL) solution were mixed, and then incubated at 37°C for 2h, each 100μL was spread on an agar plate, incubated at 37°C for 24h, and the colony on the plate was observed quantity. get as Figure 6 The comparison chart of antibacterial activity shown. Depend on Figure 6 It can be seen that the bactericidal effect of teicoplanin-nanogold is the best.

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Abstract

The invention discloses a preparation method, a product and an application of teicoplanin-functionalized gold nanoparticles. The preparation method comprises the following steps: mixing teicoplanin, chloroauric acid and water into a solution, adjusting the pH value of the solution with alkali till the pH value of the solution is in a range of 10-14 and the solution is brown, performing a stirring reaction at the temperature of 20-25 DEG C for 0.5-24 h till the solution becomes dark red, and performing dialysis to remove unreacted teicoplanin or chloroauric acid to obtain the teicoplanin-functionalized gold nanoparticles. According to the method, teicoplanin and chloroauric acid are subjected to a one-pot mixing reaction. The teicoplanin molecular structure contains hydroxyl and amino, which can be directly used as a stabilizer and reductant in the reaction. The method is quite simple and easy to operate. The teicoplanin-functionalized gold nanoparticles has better gram positive bacteria resistance performance than teicoplanin, and can be applied to the field of biological medicine.

Description

technical field [0001] The invention belongs to the field of synthesis of antibacterial drugs, and in particular relates to a preparation method of teicoplanin functionalized nano-gold as well as products and applications thereof. Background technique [0002] The outbreak of food safety and infectious diseases caused by pathogenic bacteria has become a hot issue in society and people's livelihood in recent years. The effective treatment of infectious diseases caused by bacteria mainly depends on antibiotics. Due to the abuse of various antibiotics, especially the application of third-generation cephalosporins, many strains have developed drug resistance. [0003] Teicoplanin is a glycopeptide antibiotic with strong antibacterial activity against Gram-positive bacteria. Teicoplanin has a strong lipophilicity due to its unique fatty chain, which can penetrate into tissues and cells more easily. After interacting with bacteria, it can cause the death of bacteria by hindering ...

Claims

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Application Information

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IPC IPC(8): A61K38/14A61K41/00A61P31/04B82Y30/00B82Y40/00
CPCA61K38/14A61K41/0052B82Y30/00B82Y40/00
Inventor 宋尔群余梦群宋杨
Owner SOUTHWEST UNIVERSITY
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