Production method of high-purity teicoplanin

Abstract

The invention provides a method for making high-purity teicoplanin. The method comprises the following stages that: stage a, gel chromatography and macropore fractionation by adsorption of a teicoplanin crude product solution are carried out so as to obtain a teicoplanin crude product solution; stage b, active carbon decoloring, ultrafiltration, nanofiltration and crystallization of the teicoplanin crude product solution are carried out so as to obtain high-purity teicoplanin. The method furthest increases the production efficiency of teicoplanin, thereby realizing the industrialization of high-purity teicoplanin.

Description

technical field [0001] The invention relates to a method for purifying and producing the anti-biological substance teicoplanin by using a variant strain actinoplanesteichomyceticus var jianesis; A high-efficiency method for the purification of actinomycetes to produce antibiotic substance-teicoplanin. Background technique [0002] The so-called antibiotics refer to low-molecular compounds that inhibit the reproduction of other organisms in a low-concentration environment below 1 mg / ml. Therefore, the category of antibiotics usually includes not only pharmaceutical antibiotics, but also anticancer agents, agricultural fungicides, herbicides, and insecticides; Chemical structure, etc., can also subdivide the above-mentioned antibiotics. [0003] On the other hand, about 10,000 kinds of antibiotic substances have been isolated from natural products so far; and more than 50% of these antibiotic substances are purified and produced by actinomycetes; most of which actinomycetes ...

AI Technical Summary

Problems solved by technology

In fact, the optimal growth condition for teicoplanin is a weakly alkaline state above pH 7; however, the above separation method can only isolate / purify teicoplanin in an acidic pH culture solution environment, and an acidic environment will hinder the growth of the strain Therefore, the above-mentioned separation / purification method not only has the defect of low production efficiency, but also has the problem of the self-toxicity of the bacterial strain that cannot be solved in the cultivation of the bacterial strain and the isolation process of teicoplanin

The so-called self-toxicity phenomenon refers to that the antibiotic substance-teicoplanin produced by the above-mentioned strains hinders the self-metabolism of the bacterial strain, thereby hindering the production of teicoplanin; for the generation of antibiotic substances, the above-mentioned self-toxicity phenomenon will reduce its production efficiency

[0012] In addition, existing documents [refer to Journal of Antibiotics such as M.R. Bardon (M.R.Bardon), Vol. 31, No. 3, pp. 170-177 (March, 1978)] describe that from the A method for isolating teicoplanin in the filtered fermented liquid of the genus Fibromyces; and the above-mentioned separation method includes, utilizing butanol to extract the above-mentioned fermented liquid in an acidic pH environment and implementing washing and concentrating engineering; due to the strong acidic ion exchange resin Use, there is a problem that some sugar components of teicoplanin are decomposed

If a strongly basic ion exchange resin is used, epimerization is prone to occur and biological activity will be lost