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Separation and purification method of high-purity teicoplanin

A technology for separation and purification of teicoplanin, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of difficult large-scale promotion, high production cost of nano-polymer microspheres, and unsuitability for industrial production, etc. problem, to achieve the effect of simple and easy method, stable yield and low cost

Active Publication Date: 2013-09-04
NCPC NEW DRUG RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high production cost of nanopolymer microspheres, this method is difficult to promote on a large scale and is not suitable for industrial production.

Method used

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  • Separation and purification method of high-purity teicoplanin
  • Separation and purification method of high-purity teicoplanin

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Preparation of High Purity Teicoplanin

[0025] a, take 60 grams of teicoplanin crude product, the mass percentage is 75.6%, dissolve with purified water until the concentration of teicoplanin is 5000ug / ml, adjust the pH to 7, and pass into the chromatogram filled with 3L aluminum oxide The column is used for sample loading adsorption, wherein the particle size of aluminum oxide is 200-300 mesh, and the flow rate of sample loading is 0.3BV / h, until the concentration of teicoplanin in the effluent is greater than 100u / ml; then use 1BV / h Wash with water until the effluent is colorless, then wash with methanol at a volume ratio concentration of 5%, and wash with alcohol at a flow rate of 1BV / h until the effluent reaches 3BV; then wash with water at a rate of 1BV / h until the effluent It is colorless, and then eluted with a methanol aqueous solution with a volume ratio concentration of 40%, and the elution flow rate is 0.3BV / h, and the A in the elution effluent is determined...

Embodiment 2

[0032] a, take 60 grams of teicoplanin crude product, the mass percentage is 75.6%, dissolve with purified water until the concentration of teicoplanin is 20000ug / ml, adjust the pH to 9, pass into the chromatogram filled with 3L aluminum oxide The column is used for sample loading adsorption, wherein the particle size of aluminum oxide is 200-300 mesh, and the sample loading flow rate is 0.6BV / h, until the concentration of teicoplanin in the effluent is greater than 100u / ml; Wash with water until the effluent is colorless, then use an ethanol solution with a concentration of 30% by volume for alcohol washing, the flow rate of alcohol washing is 2BV / h, until the effluent reaches 4BV; then wash with water at a rate of 2BV / h until the effluent It is colorless, and then eluted with an ethanol aqueous solution with a volume ratio concentration of 90%, and the elution flow rate is 0.6BV / h, and the A in the elution effluent is determined. 2 Group components and impurity concentration...

Embodiment 3

[0039] a, take 60 grams of crude product of teicoplanin, the mass percentage is 75.6%, dissolve it with purified water until the concentration of teicoplanin is 10000ug / ml, adjust the pH to 7.5, and pass it into the chromatogram filled with 3L aluminum oxide The column is used for sample loading adsorption, wherein the particle size of aluminum oxide is 200-300 mesh, and the sample loading flow rate is 0.5BV / h, until the concentration of teicoplanin in the effluent is greater than 100u / ml; Wash with water at the rate until the effluent is colorless, then use isopropanol aqueous solution with a volume ratio concentration of 15% for alcohol washing, the flow rate of alcohol washing is 1.5BV / h, until the effluent reaches 3.5BV; then use 1.5BV / h Wash with water at a rate of 0.5BV / h until the effluent is colorless, then elute with isopropanol aqueous solution with a concentration of 70% by volume, and measure A in the eluted effluent. 2 Group components and impurity concentrations,...

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Abstract

The invention provides a separation and purification method of high-purity teicoplanin, which comprises the steps of taking teicoplanin coarse powder as a raw material, sequentially conducting absorption, water washing, low-concentration low alcohol washing, water washing and high-concentration low alcohol elution with an aluminum oxide chromatographic column, then conducting nanofiltration concentration, decoloration, refined filtration and crystallization on an effluent, and obtaining pure teicoplanin. The method is easy and simple to operate and low in cost, and is suitable for industrial production, the obtained teicoplanin is high in purity, and the product quality complies with the EP (European Pharmacopoeia) standard.

Description

technical field [0001] The invention belongs to the field of pharmaceutical engineering, in particular to a method for separating and purifying teicoplanin obtained from microbial fermentation. Background technique [0002] Teicoplanin is an antibiotic produced by the fermentation of Actinoplanes teichomyceticus. It has strong antibacterial activity against Gram-positive aerobic bacteria and anaerobic bacteria. It is one of the drugs that still has antibacterial activity against multi-drug resistant Staphylococcus aureus and Enterococcus. [0003] The molecular weight of teicoplanin is about 1900, and it is composed of five main active components A with very similar structures 2-1 、A 2-2 、A 2-3 、A 2-4 、A 2-5 , and another active ingredient A 3-1 composition. Among them, the sum of the first 5 active components is referred to as A 2 group, another active component called A 3 Group. The general formula of the molecular structure of teicoplanin is shown in formula (1)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K9/00C07K1/36C07K1/16C07K1/30
Inventor 仲伟潭郭月玲魏增辉常亮王崔岩李敏娜张雪霞周站云倪慧敏梁冬王力群孟亚娟
Owner NCPC NEW DRUG RES & DEV
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