Teicoplanin purifying method

A technology of teicoplanin and purification method, which is applied in the field of purification of teicoplanin, can solve problems affecting the production environment and personnel health, unsatisfactory decolorization and impurity removal effects, and relatively large impact of teicoplanin control. Achieve the effect of avoiding residue problems, good decolorization effect and large preparation volume

Active Publication Date: 2017-04-26
FUJIAN INST OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although the above-mentioned resin adsorption method has been relatively fully approved in specific industrial production, it also has the following defects: the resins used in the first current resin adsorption method are mostly non-polar macroporous adsorption resins, which are not suitable for the alternative The control of the proportion of each component of lanin has a great influence, and the effect of decolorization and impurity removal is not ideal; the second is the final precipitation operation, usually by adding acetone to a concentration of 90% acetone to separate out teicoplanin, which requires a large amount of acetone, greatly aggravating the pressure of environmental protection, and affecting the production environment and personnel health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Get 10L of teicoplanin fermentation broth, adjust the pH value to 11.0, then adopt plate and frame filtration to obtain the teicoplanin filtrate; the teicoplanin filtrate is pumped into the pretreated polar large Pore ​​adsorption HT60 (20-60 mesh) resin chromatography column (Φ10×120cm); then washed with purified water until colorless, and then at an elution rate of 1.5L / h, the volume ratio was sequentially 10:90, 20: 80, 30:70, 40:60 ethanol-water solution (analysis solution) for gradient elution, and the analysis solution used for each gradient elution is 3 times the volume of the packed bed, and the enrichment solution is collected to obtain teicoplanin rich Collect liquid, adopt high performance liquid chromatography to detect this teicoplanin enriched liquid, then detection result is as follows figure 2 shown;

[0049] The obtained teicoplanin enrichment solution was diluted with an equal volume of purified water and then pretreated with a medium-polarity HN60 (...

Embodiment 2

[0051] Get 100L of teicoplanin fermentation broth, adjust the pH value to 10.0, then adopt plate and frame filtration to obtain the teicoplanin filtrate; the teicoplanin filtrate is pumped into the pretreated polar large Pore ​​adsorption HT60 (20-60 mesh) resin chromatography column (Φ25×200cm); then wash with purified water until colorless, and then use gradients at a volume ratio of 10:90 and 20:80 at an elution rate of 20L / h , 30:70, 40:60 ethanol-water solution (analysis solution) for gradient elution, and the analysis solution used for each gradient elution is 5 times the volume of the packed bed, and the enrichment solution is collected to obtain teicoplanin enrichment liquid;

[0052] The obtained teicoplanin enrichment solution was diluted with an equal volume of purified water and pretreated with a medium-polarity HN60 (100 mesh) macroporous adsorption resin column (Φ25×200cm); then washed with purified water until it was colorless, and then washed with 15 L / The el...

Embodiment 3

[0054] Get 800L of teicoplanin fermentation broth, adjust the pH value to 12.0, then adopt plate and frame filtration to obtain the teicoplanin filtrate; the teicoplanin filtrate is pumped into the pretreated polar large Pore ​​adsorption HT60 (20-60 mesh) resin chromatography column (Φ50×300cm); then wash with purified water until colorless, and then use gradients at a volume ratio of 10:90 and 20:80 at an elution rate of 60L / h , 30:70, 40:60 ethanol-water solution (analysis solution) for gradient elution, and the analysis solution used for each gradient elution is 5 times the volume of the packed bed, and the enrichment solution is collected to obtain teicoplanin enrichment liquid;

[0055] The obtained teicoplanin enrichment solution was diluted with an equal volume of purified water and pretreated with a medium-polarity HN60 (100 mesh) macroporous adsorption resin column (Φ60×250cm); then washed with purified water until it was colorless, and then washed with 40 L / The el...

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Abstract

The invention provides a teicoplanin purifying method. The purifying method includes steps of orderly performing resin gathering, resin purification, film concentration, and spraying drying operations on teicoplanin fermentation liquor. Compared with the prior art, polarity macroporous adsorbing rein is combined with a medium-sized polarity chromatography resin with uniform grain diameter, thus the product yield is high and decoloring effect is good; the spraying drying technology avoids the massive use of acetone, so that environment is protected, cost is reduced and problem of residue of organic solvent in the prior art can be further avoided; the ratio of every component of the teicoplanin obtained through the purifying method can meet standard, the purifying process is high-efficient and convenient, the preparation amount is big; the teicoplanin purifying method is applicable to industrial production.

Description

[0001] 【Technical field】 [0002] The invention belongs to the field of pharmaceutical engineering, in particular to a method for purifying teicoplanin. [0003] 【Background technique】 [0004] Teicoplanin (teicoplanin) is another glycopeptide antibiotic developed after vancomycin against drug-resistant bacteria. In the existing teicoplanin production method, generally adopt microbial fermentation to produce teicoplanin as main, and the teicoplanin in the fermented liquid comprises TAl~TA2, and wherein TA2 is the main active ingredient of teicoplanin, mainly consists of 5 It consists of two compounds (TA2-1, TA2-2, TA2-3, TA2-4, TA2-5) with very similar chemical structures; its crude and refined products also contain an active component TA3-1, which is TA2 The deacylated glucosamine product; in addition, it also contains 2 analogs with strong lipophilicity. The chemical structure of teicoplanin is shown in Formula 1 below. At present, among the standards followed in the phar...

Claims

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Application Information

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IPC IPC(8): C07K9/00C07K1/36C07K1/34C07K1/16
CPCC07K9/008
Inventor 杨煌建张祝兰连云阳王德森任林英高振云刘珣
Owner FUJIAN INST OF MICROBIOLOGY
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