Purification method of teicoplanin

Abstract

The invention provides a purification method of teicoplanin, and the purification method comprises the following steps: (1) adjusting teicoplanin fermentation liquid to be alkaline, then performing filtration; (2) adjusting the filtrate obtained in step (1) to be acidic, eluting the filtrate through macroporous adsorption resin by using water and ammonia water in order, collecting the ammonia water eluate; (3) filtering the eluate obtained in step (2), separating the filtrate through silica gel chromatography, collecting the eluate of ethanol aqueous solution with a volume ratio of 10-50%; (4) adjusting the eluate obtained in step (3) to be acidic, performing ultrafiltration by an ultrafilter membrane with a cutoff molecular weight of 5000-30000 Da, preferably 10000 Da, so as to obtain an ultrafiltrate; and (5) performing nanofiltration of the ultrafiltrate obtained in step (4) through a nanofiltration membrane with a cutoff molecular weight of 100-1000 Da, preferably 200 Da, and concentrating to obtain the teicoplanin. The purification method of the invention improves the production efficiency of teicoplanin to the maximum extent, and thus realizes industrial production of high-purity teicoplanin.

Description

technical field [0001] The invention relates to a method for purifying teicoplanin. Background technique [0002] Teicoplanin (teicoplanin) is a representative series of glycopeptide antibiotics. Clinically, it is mainly used to treat severe infections caused by sensitive bacteria such as Staphylococcus aureus and Streptococcus, such as endocarditis, osteomyelitis, sepsis, and infections of the respiratory tract, urinary tract, skin, and soft tissues. Its antibacterial mechanism is that the substance can hinder the mutual combination of peptidoglycan synthesis intermediates in the process of cell wall synthesis of Gram-positive strains, thereby inhibiting the growth of the above-mentioned bacteria. [0003] In the existing methods for producing teicoplanin, Actinomyces teicoplanos is generally used as the production strain of teicoplanin, and the crude product of teicoplanin obtained by fermentation needs to be purified. Chinese patent application 200710107185.6 discloses ...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the process is complex, the separation and purification effect is poor, the extraction yield is low, and the actual operation is difficult; the use of imported dextran gel and agarose gel for chromatography is expensive and the equipment investment is high; crystallization uses A large amount of acetone affects the production environment and personnel health