The invention discloses a Porphyromonas gingivalis culture medium for tartar specimens and a preparation method thereof. The invention is characterized in that every 1000ml of culture medium contains malt extract, egg white, hemin solution, vitamin K1 ethanol solution, magnesium citrate, agar, synercid, telithromycin, linezolid, asparagine and the balance of distilled water. The preparation method of the optimal formula comprises the following steps: dissolving the malt extract, egg white, hemin, agar, magnesium citrate, calcium citrate tetrahydrate, synercid, garlic press juice and L-cysteine in a rhizoma polygonati boiling solution, evenly mixing, adding water to the constant volume, sterilizing and packaging. Compared with the prior art, the culture medium has the characteristics of high detection reliability and high Porphyromonas gingivalis separation rate.