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Method for separating and purifying teicoplanin

A technique for separation and purification of teicoplanin, applied in the field of biomedicine, can solve the problems of large solvent usage, high production cost, expensive chromatographic packing, etc., and achieve the effect of changing appearance and color, improving effective components, and reducing side effects

Active Publication Date: 2017-11-21
鲁南新时代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large amount of solvent used, the poor separation and purification effect and the low purity of the final product prepared by the solvent extraction method, this method is rarely used at present.
Chromatography, because the chromatographic filler used is expensive and the production cost is high, it is not conducive to industrial application

Method used

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  • Method for separating and purifying teicoplanin
  • Method for separating and purifying teicoplanin
  • Method for separating and purifying teicoplanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Teicoplanin fermentation broth 25L, fermented broth titer 3200mg / L, adjust pH value to 10.8 with 1mol / L sodium hydroxide solution, filter with ceramic membrane, when concentrated to 8L, add 4L to ceramic membrane impermeable liquid Purified water, adjust the pH value to 10.8 with 1mol / L sodium hydroxide solution, stir for 5min, and continue to filter with ceramic membrane. The above operation was repeated 11 times, and a total of 44 L of purified water was added. Sampling, HPLC detection of teicoplanin 1221mg / L in the ceramic membrane permeate solution, ceramic membrane filtration ends. A total of 61L of ceramic membrane permeate was collected, containing 74.4g of teicoplanin.

[0045]Add absolute ethanol to the ceramic membrane permeate, adjust the alcohol content to 16%, adjust the pH value to 6.5 with 1mol / L hydrochloric acid, load the sample on a macroporous adsorption resin column, the resin model is HZ818, the column volume is 7L, and the resin column diameter is...

Embodiment 2

[0054] Receive 20L of fermentation broth to be put into the tank, the titer of the fermentation broth is 3720mg / L, adjust the pH value to 11 with 1mol / L sodium hydroxide solution, filter with ceramic membrane, when it is concentrated to 6L, add 3L to the impermeable liquid of ceramic membrane Ethanol with an alcohol content of 5%, adjust the pH value to 11 with 1mol / L sodium hydroxide solution, stir for 5min, and continue to filter with a ceramic membrane. The above operation was repeated 11 times, and a total of 33 L of 5% ethanol was added. Sampling, HPLC detection of teicoplanin 1421mg / L in the ceramic membrane permeate liquid, ceramic membrane filtration ends. A total of 47L of ceramic membrane permeate was collected, containing 67g of teicoplanin.

[0055] Add absolute ethanol to the ceramic membrane permeate, adjust the alcohol content to 20%, adjust the pH value to 7 with 1mol / L hydrochloric acid, load the sample on a macroporous adsorption resin column, the resin mode...

Embodiment 3

[0064] Receive 25L of fermentation broth to be put into the tank, the titer of the fermentation broth is 3521mg / L, adjust the pH value to 11.2 with 1mol / L sodium hydroxide solution, filter with ceramic membrane, when concentrated to 8L, add 4L to the impermeable liquid of ceramic membrane Alcohol is 10% ethanol, adjust the pH value to 11.2 with 1mol / L sodium hydroxide solution, stir for 5min, and continue to filter with ceramic membrane. The above operation was repeated 11 times, and a total of 44 L of 10% ethanol was added. Sampling, HPLC detection of teicoplanin 1320mg / L in ceramic membrane permeate liquid, ceramic membrane filtration ends. A total of 60L of ceramic membrane permeate was collected, containing 79g of teicoplanin.

[0065] Add absolute ethanol to the ceramic membrane permeate, adjust the alcohol content to 15%, adjust the pH value to 6.8 with 1mol / L hydrochloric acid, load the sample on a macroporous adsorption resin column, the resin model is XAD16, the colu...

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Abstract

The invention provides a method for separating and purifying teicoplanin. The method specifically comprises the following steps: filtering a teicoplanin fermentation liquid by means of a ceramic membrane; performing macroporous adsorption resin column chromatography; performing cation exchange resin column adsorption, and performing elution; performing nanofiltration on the eluate, and performing concentration; performing reverse chromatographic resin column adsorption, and performing elution; performing activated carbon decolorization; performing nanofiltration on the decolorization liquid, and performing concentration; performing ultrafiltration, performing nanofiltration, and performing concentration; and performing freeze-drying. The method overcomes the defects of the existing teicoplanin extraction technology, and improves the purity of the teicoplanin.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for separating and purifying teicoplanin. Background technique [0002] Teicoplanin (Teicoplanin) is a group of glycopeptide antibiotics produced by the fermentation of Actinomycetes teicoplanin. It is another glycopeptide against drug-resistant bacteria developed after the internationally recognized antibiotic vancomycin against drug-resistant bacteria Antibiotics, which mainly have strong antibacterial activity against Gram-positive aerobic and anaerobic bacteria, especially for infections caused by methicillin-resistant Staphylococcus aureus (MRSA). One of the most active drugs against multidrug-resistant Staphylococcus aureus and Enterococci. [0003] Teicoplanin is a mixture of various components produced by the fermentation of actinomycetes. It has a complex structure and poor stability to heat, acid, and alkali. It is easy to cause inactivation an...

Claims

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Application Information

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IPC IPC(8): C07K9/00C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16
CPCC07K9/00
Inventor 张贵民朱海洋李春利
Owner 鲁南新时代生物技术有限公司
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