Method for detecting antibacterial agent in serum by ultra-high performance liquid chromatography-tandem mass spectrometry technology

Abstract

The invention discloses a method for detecting an antibacterial agent in serum by an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The antibacterial agent containssulbactam (SBT), imipenem (IPN), linezolid (LNZ), melopenem (MPN), moxifloxacin (MXC), piperacillin (PRC), tigecycline (TGC), cefoperazone (CFZ), vancomycin (VCM) and teicoplanin (TCL). The method comprises the steps: detecting the content of the antibacterial drug in the pretreated serum by adopting an ultra-high performance liquid chromatography tandem mass spectrometry method, quantifying by utilizing a mass spectrometry isotope internal standard method, establishing a calibration curve by taking the concentration ratio of a standard substance to an internal standard substance as an X axisand the peak area ratio of the standard substance to the internal standard substance as a Y axis, and calculating the concentration of a target drug in the serum. According to the method, the pretreatment process is simple, the sensitivity is high, the specificity is high, separation and detection of the antibacterial agent are completed within 5 min, and a reliable detection method is provided for monitoring the treatment concentration of the antibacterial agent clinically.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and more specifically relates to a method for detecting antibacterial drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Bacterial infections are an important source of morbidity and mortality, and treatment with antibiotics has become an important aspect of inpatient management, especially in the intensive care unit (ICU). However, it has been proven that the blood concentration of patients treated with antibiotics based on the standard dosage regimen often cannot reach the effective therapeutic concentration, which greatly affects the therapeutic effect and may cause patients to develop resistance to a variety of bacteria. Because of interindividual variability in pharmacokinetics in different patients, simply increasing the dose of a drug can cause dangerous toxic side effects. Therefore, strict in vivo drug co...

AI Technical Summary

Problems solved by technology

[0012] At present, the methods for the determination of antibacterial drugs in human serum or plasma mainly include HPLC-UV method and LC-MS / MS method in domestic and foreign literature reports, but there are defects such as low sensitivity, long analysis time for a single sample, and large sample consumption. Quantification by external standard method, there is matrix interference, and the accuracy is poor

Chinese patents (CN 110361481 A and CN 110361482 A) disclose respectively "a kit for monitoring the concentration of vancomycin in blood and its detection method" and "a kit for monitoring the concentration of linezolid in blood and its detection method". ", the two inventions are very similar, both use multi-dimensional online solid phase extraction liquid chromatography analysis technology to measure vancomycin and linezolid in blood, the sample size used in the method is 4mL, the external standard method is used for quantification, single sample analysis The time is up to 17 minutes; China Drug Application and Monitoring, Volume 10, Issue 2, April 2013, entitled "Determination of Moxifloxacin Concentration in Plasma by HPLC-MS / MS", the method uses levofloxacin as an internal standard to detect Moxifloxacin in plasma of patients The concentration of cifloxacin, this method is no longer applicable to patients who have taken levofloxacin; Chinese Journal of Clinical Pharmacology, Volume 35, Issue 24, entitled "Simultaneous determination of voriconazole and tigecycline in human plasma by LC-MS / MS In the article "The Concentration of Drugs", other drugs were also used as internal standards for quantitative analysis, which lacked universal applicability and accuracy.

Furthermore, clinical samples are extremely precious, so the amount of pre-treated samples should be kept as small as possible, and too much sample volume will seriously pollute the instrument and increase the maintenance cost of the instrument.

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