Teicoplanin purifying method

A technology of teicoplanin and purification method, which is applied in the field of purification of teicoplanin, can solve the problems of large cost input, potential safety hazards, complicated process, etc., and achieve the reduction of subsequent environmental protection load, consumption reduction and usage reduction Effect

Inactive Publication Date: 2017-04-26
沁浩膜技术(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process has great potential safety hazards in the production process, and requires too many protective measures. The process is co...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the purification of teicoplanin

[0023] 1. Cool the teicoplanin fermentation broth to 5°C with frozen brine through the cooling coil or jacket, adjust to alkaline pH=11 with 8% NaOH, and then filter. Among them, the inorganic ceramic membrane is used for filtration, the molecular weight cut-off is 100,000MW, the pore size is 50nm, the flow channel is 4mm, the filtration temperature is 25°C, the working pressure is: inlet pressure 3bar, outlet pressure 1.8bar; the amount of water added is 2.5 times, and the concentration ratio is 3 times. The ceramic membrane retentate is discharged as sewage, and the ceramic membrane filtrate is sent to the resin.

[0024] 2. After the ceramic membrane filtrate is adjusted to pH = 6 with 2N hydrochloric acid, it is adsorbed on a resin column at a flow rate of 0.8-1.0BV / h. After the column is loaded, use 3 times the volume of purified water once at 0.8-1.0BV / h Wash with water, then wash with 3 times the volume of a soluti...

Embodiment 2

[0028] Embodiment 2: the purification of teicoplanin

[0029] 1. Cool the teicoplanin fermentation broth to 10°C with frozen brine through the cooling coil or jacket, adjust to alkaline pH=11.5 with 12% NaOH, and then filter. Among them, inorganic ceramic membrane is used for filtration, the molecular weight cut-off is 150000MW, the pore size is 100nm, the flow channel is 4mm, the filtration temperature is 44°C, the working pressure is: inlet pressure 3bar, outlet pressure 1.8bar; the amount of water added is 4 times, and the concentration ratio is 4 times. The ceramic membrane retentate is discharged as sewage, and the ceramic membrane filtrate is sent to the resin.

[0030] 2. After the ceramic membrane filtrate is adjusted to pH = 8 with 2N hydrochloric acid, it is adsorbed on the first-stage resin column at a flow rate of 0.8-1.0BV / h. After the column is loaded, use 3 times the volume of purified water to carry out once at 0.8-1.0BV / h Wash with water, then wash with 3 tim...

Embodiment 3

[0034] Embodiment 3: the purification of teicoplanin

[0035]1. Cool the teicoplanin fermentation broth to 9°C with frozen brine through the cooling coil or jacket, adjust to alkaline pH=12 with 12% NaOH, and then filter. Among them, inorganic ceramic membrane is used for filtration, the molecular weight cut-off is 200000MW, the pore size is 200nm, the flow channel is 4mm, the filtration temperature is 30°C, the working pressure is: inlet pressure 3bar, outlet pressure 1.8bar; water addition is 4 times, and the concentration ratio is 4 times. The ceramic membrane retentate is discharged as sewage, and the ceramic membrane filtrate is sent to the resin.

[0036] 2. After the ceramic membrane filtrate is adjusted to pH = 7 with 2N hydrochloric acid, it is adsorbed on the first-stage resin column at a flow rate of 0.8-1.0BV / h. After the column is loaded, use 3 times the volume of purified water once at 0.8-1.0BV / h Wash with water, then wash with 3 times the volume of a solution ...

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Abstract

The invention discloses a teicoplanin purifying method. The method comprises the following steps: cooling a teicoplanin fermentation liquid to 5-10 DEG C, adjusting the pH value of the teicoplanin fermentation liquid to 11-12, and carrying out ceramic membrane filtration to obtain a ceramic membrane filtrate; adjusting the pH value of the ceramic membrane filtrate by using hydrochloric acid, carrying out first stage resin column adsorption on the adjusted filtrate, and collecting obtained eluate; adding purified water to dilute the eluate, carrying out second stage resin column adsorption on the diluted eluate, eluting the diluted eluate with purified water, eluting the diluted eluate with methanol, and collecting obtained eluate; adding purified water to dilute the eluate, and concentrating the eluate by using a nanofiltration membrane; and carrying out active carbon decolorizing, crystallizing and drying: decolorizing the obtained nanofiltration concentrate by using active carbon, adding acetone used as a crystallization solvent, crystallizing the decolorized concentrate, and drying obtained crystals to obtain fine teicoplanin powder with the teicoplanin purity of 95% or above and the total yield of 60% or above. The purifying method has he advantages of high purity, high recovery rate and safety.

Description

technical field [0001] The invention relates to the field of antibiotic production, in particular to a method for purifying teicoplanin. Background technique [0002] Teicoplanin, also known as Taikoycin, is a glycopeptide antibiotic produced by the fermentation of actinomycetes, and has good antibacterial activity against Gram-positive bacteria, including aerobic and anaerobic bacteria. Its molecular structure, antibacterial spectrum, and antibacterial activity are similar to vancomycin. Due to the addition of fatty acid side chains in its chemical structure. The relative molecular weight increases, so it shows good characteristics in pharmacokinetics. It inhibits the same pathway of cell wall synthesis as vancomycin, interfering with the synthesis of new parts of peptidoglycan. Teicoplanin has strong lipophilicity and is easy to penetrate into tissues and cells, causing cell death by hindering the biosynthesis of cell wall. Its antibacterial spectrum is aerobic and ana...

Claims

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Application Information

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IPC IPC(8): C07K9/00C07K1/34C07K1/16C07K1/14
CPCC07K9/00
Inventor 黄桂辉郑昌辉颜文剑黄宜乾黄宜卷
Owner 沁浩膜技术(厦门)有限公司
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