244results about How to "Sensitive method" patented technology

The invention discloses a nucleic acid detection method comprising the following steps of determining an objective sequence to be detected and a target sequence to be detected; designing and synthesizing a padlock probe according to the objective sequence; preparing a rolling circle amplification primer; preparing a crosslinking probe; carrying out coupled reaction, and carrying out rolling circle amplification by utilizing the amplification primer through taking a coupled product as a template; after the rolling circle amplification is ended, detecting a rolling circle amplification product by using the crosslinking probe, and proving that the objective target sequence exists if a fluorescent signal is remarkably enhanced. A DNA (Deoxyribonucleic Acid) which can be detected by using a crosslinking probe detecting technology is amplified through the padlock probe and the rolling circle amplification reaction under the condition that a target to be detected exists, and no long-chain products can be detected by using the crosslinking probe detecting technology if no targets to be detected exist. One unit of sequence to be detected can be amplified to form hundreds of units of repetitive sequences through rolling circle amplification, and hundreds of times of signal amplification can be obtained on the basis of one unit of repetitive sequence by using the crosslinking probe detecting technology. The flexibility of the method disclosed by the invention is greatly enhanced.

A molecular diagnosing method for botrytis of tomato includes such steps as designing a pair of PCR primers specific to the pathogen of tomato botrytis, using them for PCR amplifying of the specimen to be tested, and judging if the tomato plant is suffered from botrytis. Its advantages are high speed, high sensitivity and high specificity.

The invention discloses a cutaneous sensitization detecting method based on a co-culturing mode of a three dimensional skin model and dendric cells. The cutaneous sensitization detecting method comprises the following steps : (1) preparing of the skin model in an early stage, (2) preparing and culturing of dendric cells, (3) constructing of co-culturing of the skin model and the dendric cells and exposing to-be-tested substances, (4) testing of activity of the skin model, (5) detecting of genome of the skin model, (6) detecting of secretion of lower layer cells of the co-culturing model, (7) detecting of expression of surface markers on the dendric cells, and (8) predicting of a statistic method and result. According to the cutaneous sensitization detecting method based on the co-culturing mode of the three dimensional skin model and the dendric cells, 3D skin which is reconstructed in vitro and has functions of barrier and metabolism and the dendric cells with the immunity function are combined together, a co-cultured novel experiment system is constructed and has similar functions and sensitization reaction with human bodies; the cutaneous sensitization of the to-be-tested substances can be evaluated from qualitative and quantitive aspects; and living animals and human bodies can be replaced through the method, and possible cutaneous sensitization caused by chemicals, cosmetics and drugs can be predicted directly.

The invention discloses a method for determining the content of soluble arsenic in a realgar-containing medicine and is characterized in that the method comprises the following steps of: (1) extracting soluble arsenic in the realgar-containing medicine by bionic solvent-ultrasonic treatment and / magnetic stirring; (2) alternatively purifying a solution obtained by extraction in the step (1); and (3) determining the content of soluble arsenic in the realgar-containing medicine by an ICP-MS method. The invention also discloses a method for determining the content of soluble valence-state arsenic in the realgar-containing medicine. By the adoption of the extraction mode for simulation in vivo, ICP-MS and HPLC-ICP-MS, the method for determining drug effects of realgar and main toxic components is established, and the method is sensitive and accurate.

The invention belongs to the product quality detection field, and concretely relates to a method for rapidly detecting the residual amount of benzene-based pollutants, such as benzene, toluene, ethylbenzene, xylene, styrene and the like, in plastic particles. The method concretely comprises the following steps: preparing a standard sample and an internal standard solution; pre-treating a sample; carrying out enriching extraction on benzene-based substances in the sample; carrying out GC / MS analysis; and determining the contents of the benzene-based substances through a standard addition method. The method disclosed in the invention can be used for detecting benzene-based volatile residues in products and a plurality of raw materials comprising color master batches, the plastic particles and the like. The method disclosed in the invention has the advantages of sensitivity, accuracy, environmental protection, simplicity, reliability and easy popularization, and is very effective in the determination of the benzene-based substances residual in a plurality of products and raw materials.

The invention relates to an apparatus and a method for rapidly measuring the sulfur dioxide residue level in a Chinese herb, and belongs to the technical field of drug analysis. The method comprises: a, drawing a standard curve and deriving a calculation formula; b, directly detecting the Chinese herb without treatment; and c, calculating the result. The apparatus comprises a purified water bottle, a sodium hydroxide solution bottle, an o-phthalaldehyde solution bottle, an ammonium acetate solution bottle, a sodium sulfite solution bottle, a multi-channel micro-flow-controlled peristaltic pump, a reaction pool, an ultrasound instrument, a micro-flow-controlled peristaltic pump, a serpentine condensation pipe, a filtration membrane, a cuvette, a detector, and a workstation. The method has characteristics of simple equipment, simple operation, accurate detection result, and effective detection of sulfur dioxide in the Chinese herb.

The invention relates to the field of biotechnology, and particularly relates to applications of a single-stranded nucleotide sequence of an INHA gene as animal superovulation molecule marker, and a detection method. The INHA gene of the western Hunan yellow cattle has Reference SNP Cluster Report:rs41257116 locus, and when being hybridized into A / G, the locus expresses the superovulation advantage. The partial segment at the Reference SNP Cluster Report:rs41257116 locus of the INHA gene of the to-be-detected western Hunan yellow cattle is detected by adopting a PCR-SSCP technology to determine the INHA gene of the western Hunan yellow cattle as AA or AG, the western Hunan yellow cattle with the INHA gene being AG type is selected as western Hunan yellow cattle capable of obtaining better superovulation character. According to the method, the superovulation efficiency of the western Hunan yellow cattle can be increased. The method is simple and convenient, fast and sensitive, a special instrument is not required, and the implementation is convenient.

The application discloses a video communication method and device. The method comprises the steps of: detecting a network delay, a packet loss rate and a bandwidth of a transmitted video; determininga network parameter value of the transmitted video, wherein the network parameter value is a weighted array of at least two of the network delay, the packet loss rate and the bandwidth; according to the network parameter value, determining a negotiation code rate and / or a negotiation coding format; sending the determined negotiation code rate and / or negotiation coding format to an opposite end ofvideo communication; and receiving video data processed according to the negotiation code rate and / or the negotiation coding format from the opposite end. According to the method and device disclosedby the invention, the code rate and / or the coding format of video transmission can be regulated in real time according to a network condition so as to ensure quality of video communication.

The present invention relates to the field of pharmaceutical technology, particularly to a preparation method for oligomer impurities of dextro-lipoic acid and analysis detection methods for the oligomer impurities of dextro-lipoic acid. The preparation method for the oligomer impurities comprises: taking dextro lipoic acid as a monomer; dissolving the monomer in an organic solution with low concentration; under catalysis of a free-radical initiator, effectively controlling the polymerization degree for condensation; refining and obtaining a high purity dextro lipoic acid oligomer, the HPLC purity degree of which is higher than 98.5%, by a recrystallization system of water, ethanol and methyl isoamyl ketone so as to facilitate use applications of an impurity reference substance. The present invention also provides an analysis method for measuring the oligomer impurities in the lipoic acid bulk drug or preparation. The method adopts a means of high molecular exclusion chromatography, wherein the mobile phase is phosphate buffer and acetonitrile (97: 3), the detection wavelength is 254 nm, the flow velocity is 0.8 mL / min, the temperature of the column is 25 DEG C, and the injection quantity is 20 [mu]L. The establishment of the analysis method has important significance of qualitative, quantitative analysis and control of the polymer impurities in the lipoic acid bulk drug and preparation.