34 results about "Pyrus bretschneideri" patented technology

The invention discloses a method for shortening the juvenile period of directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. The method adopts sowing and greenhouse seedling raising in the same year, and comprises the following steps: removing seed coat, carrying out gibberellin treatment, spraying GA3 in the growing period of the hybrid seedlings, conducting inversed ring-bark grafting on the hybrid seedlings sprayed with GA3, carrying out hybrid seedling terminal bud grafting, dwarfing stock, spraying chlormequat chloride (CCC), and various other technical measures, so as to shorten the juvenile period of the directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. According to the method, the Pyrus bretschneideri Rehd. hybrid seedlings can bloom and fruit in the third year after being planted, and the fruiting plant rate can be 30 percent, while the conventional Pyrus bretschneideri Rehd. hybrid seedlings bloom and fruit 7 to 8 years after being planted. Being treated with the six measures, the Pyrus bretschneideri Rehd. directive breeding hybrid seedlings can fruit in the third year after being planted, that is, the juvenile period is shortened by 4 to 5 years. Therefore, the Pyrus bretschneideri Rehd. directive breeding efficiency is greatly improved; the Pyrus bretschneideri Rehd. directive breeding period is shortened; a new way and a new method are provided for breeding of new Pyrus bretschneideri Rehd. directive breeding varieties; the application value is high.

The invention discloses a method for efficiently and rapidly regenerating adventitious buds from pear leaves. The method comprises steps as follows: acquiring sterile tissue culture seedlings, generating adventitious buds by inducing the leaves and performing primary culture and subculture on the adventitious buds, wherein an inducing medium is a combination of a basic culture medium NN69, auxin IBA and cytokinin TDZ, the leaf age is about 20-50 d, dark culture is performed for 21 d, the regeneration effect of leaves of Pyrus bretschneideri Rehd. is remarkable, the regeneration rate can reach 70.83%, the average number of regeneration buds of each leaf is 2.06, the callus occurrence rate reaches 100%, the browning rate is very low and is even 0, and demands for germplasm preservation of pear varieties and requirements for the regeneration rate of the Pyrus bretschneideri Rehd. serving as a genetic transformation material are met. Requirements for the leaves are low, the material sources are enriched, and the problem of high browning rate in the woody plant tissue regeneration process is solved. The problem that the regeneration rate of the oriental pear varieties is generally low is effectively solved, an effective material is expected to be provided for genetic transformation of the oriental pear varieties, and breakthrough of genetic transformation of pears is realized.