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Method for culturing young embryos of Pyrus bretschneideri

A technology for culturing young embryos and young embryos is applied in the breeding field of in vitro rapid propagation, which can solve the problems of not forming a mature and perfect theory and method system, and achieve the effect of improving the breeding efficiency and shortening the breeding process.

Inactive Publication Date: 2020-09-25
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on the cultivation of pear immature embryos, and there is no mature and perfect theory and method system

Method used

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  • Method for culturing young embryos of Pyrus bretschneideri
  • Method for culturing young embryos of Pyrus bretschneideri
  • Method for culturing young embryos of Pyrus bretschneideri

Examples

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Embodiment 1

[0022] Embodiment 1: a kind of pear immature embryo cultivation method

[0023] (1) explant disinfection treatment:

[0024] The immature pear fruits 5 weeks after pollination were selected ( figure 1 ), after picking, carry out low-temperature vernalization treatment at 4°C, take it out after 5 days, wash the fruit with tap water for 10 minutes, and then peel off the peel and pulp, and the obtained immature immature embryos (undeveloped seed coat) or seed-bearing The mature immature embryos (seeds) of the skin were first washed once with tap water and then washed 5 times with distilled water, and then placed on filter paper for 10 minutes, and the surface water of immature immature embryos or mature immature embryos with seed coats was blotted dry. Soak immature immature embryos or mature immature embryos with seed coats in 75% ethanol on an ultra-clean workbench for 20 seconds, wash them with sterile water for 4 times, then disinfect them with 3.3% sodium hypochlorite solut...

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Abstract

The invention discloses a method for culturing young embryos of Pyrus bretschneideri and relates to Pyrus bretschneideri. In-vitro embryos are cultured by a plant cell culture technology to break dormancy of Pyrus bretschneideri seeds and realize a higher seed germination rate. The method takes unmatured young embryos or mature young embryos of the Pyrus bretschneideri as explants, a culture technology system for in-vitro young embryos of the Pyrus bretschneideri is established by explant sterilization, young embryo culture, rooting culture, acclimatization and transplantation in order to laya foundation for establishing a regeneration system of the Pyrus bretschneideri and improving Pyrus bretschneideri varieties by genetic engineering and cell engineering. The method can significantly shorten the breeding process, improve breeding efficiency, and open up a new breeding way.

Description

technical field [0001] The invention relates to a breeding method for in vitro rapid propagation in plant biotechnology, in particular to a method for cultivating young pear embryos. Background technique [0002] Pear is one of the world's main fruits, and it is the third largest fruit in my country after apples and citrus. Pear is one of the fruit trees with the longest planting history in my country. As early as the pre-Qin period, there is a record in the "Book of Songs" that "there are buds in the mountains, and trees in the mountains". Pears are also one of the most widely planted fruit trees in my country. According to the latest statistics from the United Nations Food and Agriculture Organization (FAO) in January 2019, by the end of 2017, my country's pear cultivation area was about 960,000 hectares, with a total output of about 16.53 million tons , accounting for 69.1% and 68.4% of the world's total cultivated area and total output respectively, ranking first in the ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴巨友汤超王鹏张绍铃齐开杰谢智华
Owner NANJING AGRICULTURAL UNIVERSITY
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