145results about How to "Shorten the breeding process" patented technology

The invention relates to an LED plant illuminating system and method based on PC / mobile terminal remote control.A system remote control terminal is connected with a control processor through a cloud server, and the control processor is connected with the cloud server and a power supply module in a bi-directional mode; a sensor detection module is connected with the control processor and feeds detection conditions of the environment in a greenhouse back to the control processor, and a driving module provides steady direct currents or a pulse width modulation power source to an LED illuminating module and is used for turning on a specific lamp in an LED lamp set and adjusting the brightness of the lamp set according to instructions of the control processor.A plant growth and development cycle, the photosynthetic principle and the spectral wavelength selective absorption principle are fully considered, according to spectrum sensitivity difference of plants, intelligent data analysis of the remote control terminal is realized, intelligence of the greenhouse is achieved, and self-adaptive accurate light supplementary to different plants in different light environments is achieved.The plants are supplemented with light scientifically and reasonably, full photosynthesis of the plants is ensured, and the cultivation and harvest period of the plants is shortened.

The invention provides 5 clubroot-resistant Chinese cabbage gene CRb closely-linked molecular markers, primers and a selection method of a clubroot CRb-resistant plant. The 5 clubroot-resistant Chinese cabbage gene CRb closely-linked molecular markers comprise TCR25, TCR108, TCR116, TCR30 and TCR74. Distances of the gene CRb respectively to the TCR25, the TCR108, the TCR116, the TCR30 and the TCR74 are 0.07cM, 0.04cM, 0.08cM, 0.19cM and 0.27cM. The 5 clubroot-resistant Chinese cabbage gene CRb closely-linked molecular markers can be used for molecular marker-assistant selection of clubroot-resistant genes of brassica such as Chinese cabbage and Shanghai Bak choi. In assistant selection, the 5 clubroot-resistant Chinese cabbage gene CRb closely-linked molecular markers are used simultaneously so that theoretical selection accuracy is 100%. The 5 clubroot-resistant Chinese cabbage gene CRb closely-linked molecular markers have good repeatability, high reliability and a low detection cost and saves time and labor.

The invention discloses a method used for quickly breaking dormancy of a peony seed epicotyl. With the method, the dormancy of the peony seed epicotyl supposed to naturally germinate in two years can be quickly broken, and the peony seed can germinate smoothly after being processed for three months; under the isolated culture condition, the dormancy characteristic of the peony seed epicotyl can be broken after the processing period of two weeks. With the adoption of the two methods disclosed by the invention, high-quality peony seedlings can be obtained; the peony nursery stock germinating quickly after processing is highly consistent with the phenotype of peony seedlings obtained by the traditional method, in other words, the parental genetic phenotype is maintained, and the seed germination, seedling and flow formation processes are greatly shortened; besides, the method can be used for creating heterogeneous parental new germplasm, so that the efficiency of traditional breeding and cross breeding of peony is improved, and the breeding and genetic improvement processes of peony are shortened.

The invention belongs to the field of molecular breeding of livestock, and relates to a polygene pyramiding early-breeding method for raising the litter size of sheep. Cloning is performed from FecB, BMP15 and FSHR genes so as to obtain three associated gene segments, and the nucleotide sequences of the gene segments are shown in SEQ ID NO: 1-3. A base substitution of A111-G111 exists at the 111(th) bp of SEQ ID NO: 1, which causes the digestion polymorphism of Ava II-RFLP; a base substitution of C35-T35 exists at the 35(th) bp of the SEQ ID NO: 2, which causes the digestion polymorphism of Hinf I-RFLP; and a base substitution of C88-T88 exists at the 88(th) bp of the SEQ ID NO: 3, which causes the digestion polymorphism of BsiE I-RFLP. The molecular marker combination can significantly improve the polygene pyramiding early-breeding efficiency for raising the litter size of sheep.

The invention discloses a co-separation molecular marker TCR540 of a clubroot resistant CRb gene of Chinese cabbages. A nucleotide sequence of the co-separation molecular marker TCR540 is represented as SEQ ID NO.1, TCR540 and the CRb gene are co-separated, and TCR540 can be applied to molecular marker assisted selection of the clubroot resistant CRb genes of Brassica including Chinese heading cabbages and green pakchoi. The markers are used simultaneously in an assisted selection process, the theoretical accuracy of the selection can be up to 100%, and the co-separation molecular marker TCR540 is good in repeatability, high in reliability and low in detection cost and saves time and labor.

The invention provides a seedling growing method for polygonatum sibiricum seeds. The method comprises the following steps: selection, soaking, fine sand treatment, storage, germination acceleration,seeding and seedling-stage management. According to the method provided by the invention, the polygonatum sibiricum seeds are subjected to soaking and fermentation, cold storage on a culture medium and breeding in soil, then the hibernation period of the polygonatum sibiricum seeds can be greatly shortened, and the breeding process is thus shortened; the breeding process is simple, a large quantity of polygonatum sibiricum seedlings can be provided quickly, the tuber propagation of polygonatum sibiricum is reduced, the planting cost is lowered, artificial planting of polygonatum sibiricum is promoted, excavation of wild polygonatum sibiricum is reduced, and the income of the planters is increased; moreover, through the breeding method provided by the invention, the germination rate of theobtained polygonatum sibiricum seeds is relatively high, thus popularization is facilitated.

The invention discloses an SNP marker related to growth traits of black-bone chickens and an application thereof. The invention firstly provides an application of a substance for detecting polymorphisms or genotypes of an SNP marker rs317682933 in identification or auxiliary identification of chicken body weight. The SNP marker rs317682933 is the 73209189th nucleotide of 4th chromosome located ingalGalGal6-edition chicken genome, and the alleles of the marker are C and T. The invention further provides a method for identifying or assisting in identifying chicken body weight. The invention discovers that; when the genotype of the SNP marker rs317682933 in the chicken genome is CC, the body weight is larger than that of chickens whose genotype is CT, and the correlation between the marker and the body weight is gradually enhanced along with the increase of the age of the chickens. Therefore, the application of the SNP marker rs317682933 in the growth performance breeding of the Lueyangblack-bone chickens can improve the selection accuracy, increase the selection reaction and shorten the breeding process.

The invention discloses a rice granule shape gene qSS7 as well as a preparation method and application. The sequence of the rice granule shape gene qSS7 is as shown in SEQIDNO.1. Due to the gene, the granule length and the length to width ratio can be increased, the granule width can be reduced, and the chalkiness rate and the chalkiness degree can be also reduced; and when the appearance quality of rice is improved by using the gene, the quality and the yield of rice can be prevented from negative influence; a gene mark is also designed for qSS7, long and short granule allele can be identified in the seedling period, unexpected heterozygous and pure short granule genotype plants can be rejected, the possibility that descendant granule shapes can be separated when being bred is avoided, and breeding procedures for a next season can be reduced. Therefore, the area of a descendant bred material can be reduced by 75% in quality breeding practice, the gene selection accuracy can be improved, and the breeding progress can be accelerated.

The invention discloses a method for breeding sexual triploids of colored zantedeschia by using 2n pollen, and belongs to the technical field of plant ploidy breeding. The method comprises the following steps of: (1) determining a 2n pollen induced appropriate development stage; (2) preparing styles to be treated; (3) inducing the 2n pollen by colchicine treatment; (4) identifying the 2n pollen; (5) collecting the 2n pollen; (6) performing sexual polyploidy hybridization; and (7) identifying sexual triploid descendants. By the method, the sexual triploids of the colored zantedeschia can be successfully obtained in one step.

The invention discloses a KASP marker typing primer combination for high-throughput detection of mutation sites of an AhFAD2B gene and an application of the primer combination, belongs to the technical field of molecular genetic breeding and relates to a special primer combination for detecting types of the AhFAD2B gene of high-oleic-acid peanuts by a high-throughput SNP molecular marker and the application of the primer combination. The primer combination is the KASP marker typing primer combination for detecting C>T mutant sites at 814 sites of the AhFAD2B gene of high-oleic-acid peanuts andcomprises an A-labeled wild type AhFAD2B gene specific primer with a nucleotide sequence shown in SEQ ID No.1, a B-labeled mutant AhFAD2B gene specific primer with a nucleotide sequence shown in SEQID No.2 as well as a AhFAD2B gene general primer with a nucleotide sequence shown in SEQ ID No.3. The high-throughput KASP molecular marker gene typing specific primers are designed for novel mutant 814 C>T allelic variation of the AhFAD2B of high-oleic-acid peanuts, novel mutants of the AhFAD2B can be effectively identified, and the problem that identifying methods in the prior art can only identify 448 G>A allelic variation of classic F435 mutant sites of the AhFAD2B is solved.

The invention discloses a hybridizing, sowing and seedling raising method of pears. The method comprises steps of field hybridization, fruit harvesting and after-ripening storage, seed taking, inoculated culture, seedling transplantation with nutritive bowls, field planting and the like. Compared with a traditional field seedling raising method in the prior art, the method has the advantages that indoor sterile culture media are used for direct sowing and seedling raising, and a large quantity of time and labor force can be saved. Meanwhile, with the adoption of the technology, seedling damping-off can be avoided, seedlings emerge regularly, the seedling rate is high, the number of hybrid progeny single plants is guaranteed, the hybrid seedling juvenile period is shortened, and the breeding process is accelerated.

The invention discloses a method for recurrently and selectively breeding non-glutinous rice by using a recessive cytoblast sterile material, which mainly comprises the following steps: hybridizing a sterile strain with recessive cytoblast sterile gene ms-np serving as a female parent and 20 to 30 rice materials serving as male parents, and backcrossing one generation; then, selecting seeds on excellent sterile strains in the later generations, and equivalently mixing the seeds to form a first recurrent selective population; and repeating the recurrent selection process, selecting the excellent fertile strains to a pedigree method at any time, combining molecular marker-assisted selection, and breeding a new variety. The method has the advantages that: recessive cytoblast sterility is used for performing a large amount of hybridization so as to enrich the hereditary basis of the breeding material; new rice materials can be added at any time so as to continually improve the population; moreover, the method avoids manual castration and pollination so as to save a large amount of manpower; and by utilizing a DNA molecular marker for assisting in selection, the method shortens the breeding progress and time, and reduces the breeding cost.

The invention discloses a molecular marker of a high erucic acid gene in Brassica napus L. and a breeding method thereof and relates to the biological breeding field. The method comprises the following steps of: S1, constructing a segregating population; 2, extract that genomic DNA of the material to be tested by the CTAB method; 3, carry out PCR amplification; S4, using marker primer pairs Pb1, Pb2, Fa1, Fa2 to detect whether a single plant in the segregated population contains root swelling resistance site PbBa8.1 or low erucic acid gene fae1 and classify; S5, a new rapeseed variety with high erucic acid and root swelling resistance locus PbBa 8.1 and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and high erucic acid and low erucic acid were selected. The method for selecting and breeding rapeseedwith root swelling disease resistance and low erucic acid in grain can be used for auxiliary selection of root swelling disease resistance site PbBa8.1 and low erucic acid gene fae1 with genetic linkage at seedling stage, The theoretical accuracy of gene-specific markers reached 100%, which improved the breeding efficiency of rapeseed resistant to root swelling and low erucic acid, and shortenedthe breeding process.

The invention discloses a KASP labeled primer for detecting a Wx-D1 gene in waxy wheat K107Wx1 and application of the KASP labeled primer, and belongs to the technical field of molecular genetic breeding; and three groups of KASP primers provided by the invention, when used for detecting the Wx-D1 genotype in the waxy wheat K107Wx1, are low in human error and high in analysis throughput, and are suitable for detecting a great amount of samples. A codominant molecular marker provided by the invention, when applied to transfer breeding of the Wx-D1 gene in the waxy wheat K107Wx1, has an important practical significance for accelerating genetic improvement by virtue of the Wx-D1 gene in the waxy wheat K107Wx1 and f improving a breeding efficiency.

The invention provides two SCAR marks including an SCR01-378 and an SCR02-208 which are positioned at two sides of a genetic sterile multiple allele Ms of a celery cabbage and are in tight linage with the genetic sterile multiple allele Ms. The genetic distances of the two marks and the MS gene are 1.2 centimeters and 2.5 centimeters respectively. According to the nucleotide sequences of the two marks, two pairs of specific amplification primers are designed respectively and are used for molecular-assisted selection of the genetic sterile multiple allele of the celery cabbage. The two marks are used simultaneously in the process of the molecular-assisted selection, and the selection accuracy is over 97.5 percent. The two marks have the advantages of good repeatability, high reliability, low detection cost, time conservation and labor conservation.

The invention discloses a molecular marker which is used for corn tripsacum monosome addition line nucleic male sterility genes and belongs to the field of crop molecular markers. The molecular marker is composed of nucleotide sequences shown by SEQ ID No:1. The invention further discloses an SCAR primer pair used for amplifying the molecular marker and composed of SEQ ID No:2 and SEQ ID No:3. In addition, the invention further discloses a method of using the molecular marker for backcross and recurrent selection breeding. The molecular marker is not limited by time and environment conditions, can identify and screen the corn tripsacum monosome addition line nucleic male sterility genes at the seedling stage, selects and weeds out the nucleic male sterility genes easily, saves manpower and material resources in large quantities, improves the breeding efficiency, shortens the breeding process and time, and substantially reduces breeding cost. In addition, the molecular marker can serve as a tool for large-scale recurrent selection for corns and continuously extend the corn heredity basis and is beneficial for selecting out a breakthrough corn variety.

The invention discloses a protein related to pollen grain germination and / or pollen tube growth, the encoding genes and the application thereof. The protein is the following protein shown in (1) or (2): (1) the protein being composed of amino acid sequences shown in sequence two of a sequence table; (2) the protein comprises the amino acid in the sequence two of the sequence table substituted and / or lacked and / or added and is related to the stress tolerance of plant. The protein and the encoding gene thereof related to the pollen grain germination and / or pollen tube growth can be used for the genetic transformation research of the plant, increases the pollen germination rate of plant and / or the growth speed of the pollen tube and the reproductive efficiency of the plant, shortens the breeding process, has important theoretical and practical actual meaning for adjusting and controlling the reproductive development and gene engineering breeding of woody plants with slow growth development and accelerating the genetic improvement of the woody plants.

The invention provides an anther culture method of primula forbesii, comprising selection of the explant, disinfection and vaccination of the explant, induction and proliferation of the callus, differentiation of the callus, and rooting culture and transplantation of the tissue culture seedling, wherein, the medium of the induction and proliferation of the callus is as follows: MS +6- BA1.0mg / L +2,4-D 0.5mg / L, 30g / L sucrose, 6g / L agar, pH 5.9-6.0; the differentiation medium of the callus is as follows: MS +6- BA 0.2mg / L + NAA 0.01mg / L, 40g / L sucrose, 7g / L agar, pH 5.9-6.0; the rooting medium is as follows: MS, pH 5.9-6.0. The seedling obtained through the method provided by the invention has good growth, strong consistency and adaptability, less pests and diseases, easy management, and is easy for scale production.

The invention provides a method for identifying a self-sterile S-gene type SSR molecular marker of a sweet cherry variety. The method uses PTCR4SSR to mark, wherein the SSR primer sequence is upstream primer 5'-CATAGGTTCAAACCATACCCGTG-3' and downstream primer 5'-CTCATCTTTGTAGGGTATAATACC-3' to amplify the DNA of sweet cherry of different varieties (systems), if the amplified fragment of 255bp can be amplified, pollen S-gene exists, and a electrophoretogram can be obtained by agar gel electrophoresis; according to the agarose elelctrophoretogram, the self-sterile gene type of each variety (system) can be determined, wherein the same bands are the same S-gene type, otherwise being different S-gene types. The SAM method is used to develop SSR marked PTCR4 in sweet cherry for identifying the S-gene type of different sweet cherry self-sterile gene type varieties (systems) and guiding selection of breeding parents and configuration of pollination varieties thereof, so that the detection efficiency of the self-sterile gene type can be improved, the breeding process of the sweet cherry is shortened, and the production cost is saved.

The invention relates to a method of a molecular authenticating technique for the indica-japonica of old species of Yunnan Rice, which belongs to the breeding field in the technical field of agricultural biotechnology. The technical proposal is as follows: five grains of the material seeds to be authenticated are taken for germinating under the temperature of 20 to 35 DEG C; after the seeds geminate for five days, the leaves are taken for extracting the total DNA of the rice; the concentration of the DNA is detected and the DNA is quantificationally diluted to 25ng / ul; a specific primer JI designed according to the sequence of the specific long-arm section of the 10th chromosome of the rice is used for carrying out PCR reaction; gel electrophoresis is carried out on the 2 percent agarose (containing EtBr0.1ug / ml); the PCR product is detected on a UV transilluminator; and the indica-japonica characteristics of the material are authenticated according to the electrophoresis result. The indica type rice has two PCR products with the molecular weights being respectively 280bp and 380bp; and the japonica rice has one PCR product with the molecular weight of 280bp, thus authenticating the indica-japonica characteristics of the old species of Yunnan Rice. The method has the effects of being quickly and effectively authenticating the indica-japonica characteristics of the old of Yunnan Rice.

The invention belongs to the field of detection of genetically modified plants, and particularly discloses 2 molecular markers for identifying the specificity of bivalent herbicide resistance gene cotton strains GGK2 and derived lines. Each molecular marker consists of nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The invention further discloses specific primers for amplifying the molecular markers, and purposes of the molecular markers and the specific primers. Accurate and reliable molecular markers and specific primers are provided for detection and identification of the bivalent herbicide resistance gene cotton strains GGK2 or derived materials thereof, and transgenic safety is guaranteed; then the detection method is simple, the detection speed is high, large-scale identification and screening are appropriately performed on homozygosis / heterozygote breeding materials in bivalent herbicide resistance genes in filial generations in heredity and breeding of genetically modified crops, shortening of a breeding process is facilitated, and the breeding cost is reduced.

The invention discloses a rapid breeding method of potatoes. Screening is conducted by methods of screening in Shandong and Inner Mongolia as well as screening spring, summer and autumn, varieties suitable for a central plain two-season crop area and a northern one-season crop area simultaneously can be selected, and potato new varieties only suitable for the central plain two-season crop area toplant or only suitable for the northern one-season crop area to plant also can be selected, so compared with the traditional breeding method, the rapid breeding method of the potatoes has the following advantages: the prepared hybridized combination is completely utilized and waste of hybridized materials is avoided; and through generation adding planting of two places, the operation from preparation of hybridized combination in the first year to variety registration totally needs 11 years, the breeding year limit is shortened and the breeding flow is accelerated, so that the pace of pushing the new varieties to the market is accelerated.

Provided is a method for breeding seeds of polygonatum cyrtonema Hua. The method comprises the following steps of: (1) putting the seeds of polygonatum cyrtonema Hua in a water-containing bucket for immersion and fermentation, wherein the fermentation time is 25-35 days, and the water temperature is 25-29 DEG C; (2) cleaning the fermented seeds of polygonatum cyrtonema Hua with clear water, layingthe cleaned seeds of polygonatum cyrtonema Hua on a culture medium, and then performing cold storage on the culture medium in an aerobic-environment refrigerating room, wherein the time of cold storage is 25-35 days, the temperature of the refrigerating room is 2-6 DEG C, the moisture content of the air in the refrigerating room is 11-18%, and the refrigerating room is internally provided with acold-light lamp; and (3) sowing the refrigerated seeds obtained in the cold environment to loose soil for seedling cultivation. According to the method for breeding the seeds of polygonatum cyrtonemaHua, not only can a high germination rate of the seeds of polygonatum cyrtonema Hua be ensured, but also the hibernation period of the seeds of polygonatum cyrtonema Hua is shortened, so that the seedbreeding process is shortened, the breeding process is simple, and the method for breeding the seeds of polygonatum cyrtonema Hua is easy to popularize and apply.

The invention discloses a selection breeding method of a new homozygous sterile line of brassica napus; according to the method, a F2 fertile plant to a transfer material is used for inbreeding; as for F3, a part of the F2 inbred single plants is hybridized with a temporary maintainer line for seed production so as to identify the F2 inbred single plant material with a homozygous sterile genotype; then the homozygous sterile line is separated and selected by the reserved single plant material; and productivity identification is performed on hybrids prepared by a restorer with seeds obtained in hybrid seed production by the selected material and the temporary maintainer line during fertility identification. The method saves a lot of labor force used in plantation and hybridization of the selected colony during selection, can predict the combining ability of the selected material at an early stage, and thus improves the breeding efficiency.

The invention discloses a breeding method of a new high-yield lodging-resistant disease-resistant wheat variety. The method comprises the following steps: hybridizing and backcrossing a dwarf, large-ear and hard-stalk lodging-resistant wheat intermediate material serving as a female parent and a disease-resistant variety Yangmai 18 serving as a male parent for one time, adding generations in a greenhouse, selecting single plants for inducing powdery mildew and resisting powdery mildew in the generation adding process, then performing molecular marker selection by combining synergistic sites QGns-YY-5B / QGbs-YY-5B of the number of grains per spike and the number of fruiting grains per spike base, planting selected strains into plant rows in a field, performing artificial induction selection on powdery mildew and gibberellic disease of advanced generations, and selecting comprehensive agronomic traits, stalk toughness lodging resistance and disease resistance. The new wheat variety bred by the method disclosed by the invention stably inherits the gibberellic disease and powdery mildew resistance of Yangmai 18, the excellent characters of good stem toughness and good lodging resistance of a non-recurrent parent are added, and the average yield is remarkably higher than that of the Yangmai 18.

The invention discloses a method for improving the moisture resistance of cabbage type rape. The method comprises the following steps of: (A), constructing a glufosinate-ammonium resistant expression vector containing vitreoscilla hemoglobin (vgb), namely, (1) obtaining a target fragment, (2) recovering a polymerase chain reaction (PCR) product, transforming and sequencing, (3) preparing transferred-deoxyribonucleic acid (T-DNA), (4) preparing Vector, (5) preparing the expression vector, and (6) preparing bacterial liquid of agrobacterium tumefaciens; (B), breeding a recovery line of the moisture-resistant cabbage type rape, namely transforming the vgb into R2 by an agrobacterium tumefaciens mediated method to obtain a T0 recovery line, and performing continuous selfing to obtain a T2 recovery line; (C), breeding a maintainer line of the moisture-resistant cabbage type rape, namely (a) transforming the vgb into 6098B by the agrobacterium mediated method to obtain a T0 maintainer line, and performing continuous selfing to obtain a T2 maintainer line, and (b) hybridizing a T1 recovery line single plant serving as a male parent with the 6098B, performing continuous backcrossing by taking the 6098B as a recurrent parent to obtain BC3, and performing selfing to obtain the T2 maintainer line; and (D), obtaining a sterile line of the moisture-resistant cabbage type rape. The method is easy and convenient to operate and obvious in effect, the cost can be reduced, and the breeding progress can be shortened.

The invention provides two microsatellite markers related to rapid growth of pseudosciaena crocea, and preparation methods thereof, and relates to molecular breeding of the pseudosciaena crocea. The two microsatellite markers related to the rapid growth of the pseudosciaena crocea are LYC0088 and LYC0143. Primer sequences of the LYC0088 are shown as SEQUENCE LISTING 1 and SEQUENCE LISTING 2; and primer sequences of the LYC0143 are shown as SEQUENCE LISTING 3 and SEQUENCE LISTING 4. The preparation methods comprise the steps of 1) screening microsatellite markers related to the rapid growth; 2) preliminary verifying the microsatellite markers related to the rapid growth; and 3) verifying again. The two microsatellite markers related to the rapid growth of the pseudosciaena crocea can be applied in selective breeding of body length, body height and body mass of the pseudosciaena crocea and in screening of the microsatellite markers related to the rapid growth of the pseudosciaena crocea.

The invention discloses a haploid breeding method of dendrobium candidum, which comprises the following steps: a. taking dendrobium candidum flowers blooming for three to six days, inoculating pollen at normal temperature, treating for 45-50 hours at 4-DEG C low temperature in anther callus inducement culture medium, and then carrying out conventional culture; b. sterilizing for 15 minutes with NaCIO solution, peeling out anther from the sterilized flower buds under sterile environment, inoculating into the anther callus inducement culture medium, and sealing with a preservative film; c. carrying out dark constant-temperature culture for 2-3 days at 25 DEG C, then carrying out dark shaking culture for 12-15 days to obtain an embryoid; d. collecting the embryoid, inoculating to a differential medium, to obtain haploid plant; and e. conducting doubling germination on the root of the haploid plant with colchicine. According to the haploid breeding method, the problems of storing the wild dendrobium candidum strain and developing high-quality strains can be well solved; the variety of the dendrobium candidum can be improved, and the new variety of dendrobium candidum can be bred.

The invention relates to the technical field of plant molecular biology, in particular to a corn waxy gene mutant as well as a molecular marker and application thereof. The invention provides a corn waxy protein mutant, and compared with a wild WAXY protein, the protein mutant comprises mutation that an amino acid sequence represented by SEQ ID NO.1 is inserted behind the 125th amino acid resulting in early termination of protein translation, so that protein translation terminates early. The invention also provides a corn waxy gene mutant, and compared with a wild waxy gene, the gene mutant comprises mutation that a nucleotide sequence shown as SEQ ID NO.4 is inserted behind the 43rd nucleotide of the 3rd exon. According to the WAXY protein mutant and the waxy gene mutant provided by the invention, the amylopectin content of corn grains can be remarkably improved, and the grains have waxy characters. The invention further provides the molecular marker of the waxy gene mutant, seedlingstage selection of waxy corn can be achieved, and the breeding period is greatly shortened.

The invention provides a method for rapidly stabilizing rice backcross introgression population trait. The method comprises the following steps: establishing selective introgression lines; screening the selective introgression lines; performing genotype identification on the selective introgression lines; analyzing the descendant donor introgression frequency and heterozygous genotype of the selective introgression lines; and breeding new variety of the selective introgression lines, performing regional tests on the selective introgression lines with obvious target performances under different ecological conditions at home and abroad, identifying the general performance of the variety, and approving or registering the bred new variety.