Hybridizing, sowing and seedling raising method of pears
A technology for sowing seedlings and pear trees, which is applied in the field of pear hybrid seeding and seedling raising, can solve the problems of uneven seedling emergence, low germination rate, and damping-off seedling-raising cycle at the seedling stage, and achieves uniform seedling emergence, shortens the seedling-raising process, and avoids damping-off at the young stage. effect of disease
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Embodiment 1
[0036] Embodiment 1: the impact of different treatment methods on pear seed germination
[0037] Test location: in the pear resource nursery of the Institute of Horticulture, Jiangsu Academy of Agricultural Sciences
[0038] Test species:
[0039] The research object is the Cuiguan pear fruit, which is harvested when the fruit is 7-8 years old (both are bagged fruits), and transported back to the laboratory on the day of harvest, and then the fruit without diseases and insect pests is selected for each test treatment at room temperature (25°C).
[0040] experiment method
[0041]①Determination of conventional fruit storage on germination rate and emergence of pear seeds
[0042] On 1, 7, 14 and 21d, 4 stages of treatment were taken, 100 seeds in each group, and 3 repetitions per treatment. The fruit rot rate was investigated when the seeds were taken in different periods, and then the seeds were washed and dried, and the seeds were stored in sand for 3 days. 2 months in the...
Embodiment 2
[0065] Inoculation and transplanting test of indoor culture medium
[0066] The test fruit is the Cuiguan pear fruit in the ② test method adopted in Example 1, and the storage and seeding time is the 21st day.
[0067] experiment method:
[0068] The fruit is sterilized with 20% sodium hypochlorite water-soluble for 20-30 minutes, and then the seeds are taken on the ultra-clean workbench. Select the seeds with full grains and immerse them in sterile water to prepare 20% sulfuric acid for 10-30 seconds, take them out and rinse them with sterile water for 2-3 times, then put the seeds in test tubes (Φ30×150, medium depth 20-40cm, one tube per tube) Seeds) in the dark and light cultures.
[0069] Medium preparation:
[0070] Treatment 1: MS medium
[0071] Treatment 2: MS medium + 1g / L activated carbon
[0072] Treatment 3: MS medium + 0.5mg / L GA 3
[0073] Add 25g / L sucrose+5.0g / L agar powder to each medium, pH 5.8.
[0074] ①Effect of different storage time on seed germ...
Embodiment 3
[0080] Culture medium inoculation improvement and verification
[0081] For the cultivation of ① treatment 3 in implementation 2, the modified MS medium is 0.2mg / L IBA+0.5mg / L GA 3 + 1g / L activated carbon + 25g / L sucrose + 5.0g / L agar powder, pH 5.8, the inoculation method is the same as that in Implementation 2, place the inoculated test tube in a 3°C freezer for 30 days, and then place it at 25°C , 1500-2000 lux group culture room for cultivation, germination rate statistics, 40 days for seedling growth potential measurement, and then for nutrient pot transplanting, 30 seeds a group, repeated three times.
[0082] The test tube seedlings were transplanted with a black plastic nutrient bowl with a diameter of 8 cm and a height of 12 cm. The nutrient soil was clay, peat soil, and vermiculite. The ratio of mass to mass was 1:3:1. When transplanting seedlings, it was necessary to cover them with a sunshade net for 1 week;
[0083] Field transplanting or nutrient pot seedling cu...
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