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A kind of pear hybrid sowing seedling raising method

A technology for sowing seedlings and pear trees, which is applied in the field of pear hybrid seeding and seedling raising, can solve the problems of uneven seedling emergence, damping-off seedling-raising cycle at the seedling stage, low germination rate, etc., achieves uniform seedling emergence, shortens the seedling-raising process, and avoids damping-off in the young stage. effect of disease

Active Publication Date: 2018-05-25
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for pear hybrid sowing and seedling raising, so as to solve the problems of low germination rate, irregular seedling emergence, serious damping-off disease at the seedling stage and long seedling raising cycle in the prior art.

Method used

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  • A kind of pear hybrid sowing seedling raising method
  • A kind of pear hybrid sowing seedling raising method
  • A kind of pear hybrid sowing seedling raising method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the impact of different treatment methods on pear seed germination

[0037] Test location: in the pear resource garden of the Institute of Horticulture, Jiangsu Academy of Agricultural Sciences

[0038] Test species:

[0039] The research object is the Cuiguan pear fruit, which is harvested when the fruit is 7-8 years old (both are bagged fruits), and transported back to the laboratory on the day of harvest, and then the fruit without diseases and insect pests is selected for each test treatment at room temperature (25°C).

[0040] experiment method

[0041]①Determination of conventional fruit storage on germination rate and emergence of pear seeds

[0042] On 1, 7, 14 and 21d, 4 stages of treatment were taken, 100 seeds in each group, and 3 repetitions per treatment. The fruit rot rate was investigated when the seeds were taken in different periods, and then the seeds were washed and dried, and the seeds were stored in sand for 3 days. 2 months in the ...

Embodiment 2

[0065] Inoculation and transplanting test of indoor culture medium

[0066] The test fruit is the Cuiguan pear fruit in the ② test method adopted in Example 1, and the storage and seeding time is the 21st day.

[0067] experiment method:

[0068] The fruit is sterilized with 20% sodium hypochlorite water-soluble for 20-30 minutes, and then the seeds are taken on the ultra-clean workbench. Select the seeds with full grains and immerse them in sterile water to prepare 20% sulfuric acid for 10-30 seconds, take them out and rinse them with sterile water for 2-3 times, then put the seeds in test tubes (Φ30×150, medium depth 20-40cm, one tube per tube) Seeds) in the dark and light cultures.

[0069] Medium preparation:

[0070] Treatment 1: MS medium

[0071] Treatment 2: MS medium + 1g / L activated carbon

[0072] Treatment 3: MS medium + 0.5mg / L GA 3

[0073] Add 25g / L sucrose+5.0g / L agar powder to each medium, pH 5.8.

[0074] ①Effect of different storage time on seed germ...

Embodiment 3

[0080] Culture medium inoculation improvement and verification

[0081] For the cultivation of ① treatment 3 in implementation 2, the modified MS medium is 0.2mg / L IBA+0.5mg / L GA 3 + 1g / L activated carbon + 25g / L sucrose + 5.0g / L agar powder, pH 5.8, the inoculation method is the same as that in Implementation 2, place the inoculated test tube in a 3°C freezer for 30 days, and then place it at 25°C , 1500-2000 lux group culture room for cultivation, germination rate statistics, 40 days for seedling growth potential measurement, and then for nutrient pot transplanting, 30 seeds a group, repeated three times.

[0082] The test tube seedlings were transplanted with a black plastic nutrient bowl with a diameter of 8 cm and a height of 12 cm. The nutrient soil was clay, peat soil, and vermiculite. The ratio of mass to mass was 1:3:1. When transplanting seedlings, it was necessary to cover them with a sunshade net for 1 week;

[0083] Field transplanting or nutrient pot seedling cu...

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Abstract

The invention discloses a hybridizing, sowing and seedling raising method of pears. The method comprises steps of field hybridization, fruit harvesting and after-ripening storage, seed taking, inoculated culture, seedling transplantation with nutritive bowls, field planting and the like. Compared with a traditional field seedling raising method in the prior art, the method has the advantages that indoor sterile culture media are used for direct sowing and seedling raising, and a large quantity of time and labor force can be saved. Meanwhile, with the adoption of the technology, seedling damping-off can be avoided, seedlings emerge regularly, the seedling rate is high, the number of hybrid progeny single plants is guaranteed, the hybrid seedling juvenile period is shortened, and the breeding process is accelerated.

Description

technical field [0001] The invention belongs to the field of agricultural production, and in particular relates to a pear hybrid sowing seedling raising method. Background technique [0002] Pear is a perennial woody plant. Its fruit is delicious and has rich nutritional value. It is one of the most popular fresh fruits. In the past ten years, my country has bred more than 50 fine new sand pear varieties with high yield, resistance to diseases and insect pests and other adverse external conditions, such as Cuiguan, Huangguan, Crispy Green, Huasu, Zhongli No. 1, etc. Most of these varieties are bred through conventional cross-breeding methods, that is, the pollen is collected in the field and then dried in an incubator. When pollinating, the pollen is mostly collected with a pencil tip. After pollination, the pollen is isolated in a paper bag. After the fruit is ripe, Seed collection, sand deposit, sowing seedlings, transplanting, economic traits identification for 3 consecu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00A01H1/02A01H4/00A01C1/00
CPCA01C1/00A01G17/005A01H1/02A01H4/00
Inventor 蔺经杨青松李晓刚王中华李慧常有宏
Owner JIANGSU ACAD OF AGRI SCI
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