Molecule technical method for identifying indica/japonica type of Yunnan rice old variety of Yunnan rice old variety

Abstract

The invention relates to a method of a molecular authenticating technique for the indica-japonica of old species of Yunnan Rice, which belongs to the breeding field in the technical field of agricultural biotechnology. The technical proposal is as follows: five grains of the material seeds to be authenticated are taken for germinating under the temperature of 20 to 35 DEG C; after the seeds geminate for five days, the leaves are taken for extracting the total DNA of the rice; the concentration of the DNA is detected and the DNA is quantificationally diluted to 25ng / ul; a specific primer JI designed according to the sequence of the specific long-arm section of the 10th chromosome of the rice is used for carrying out PCR reaction; gel electrophoresis is carried out on the 2 percent agarose (containing EtBr0.1ug / ml); the PCR product is detected on a UV transilluminator; and the indica-japonica characteristics of the material are authenticated according to the electrophoresis result. The indica type rice has two PCR products with the molecular weights being respectively 280bp and 380bp; and the japonica rice has one PCR product with the molecular weight of 280bp, thus authenticating the indica-japonica characteristics of the old species of Yunnan Rice. The method has the effects of being quickly and effectively authenticating the indica-japonica characteristics of the old of Yunnan Rice.

Description

technical field [0001] The invention relates to a molecular technology method for identifying indica and japonica rice varieties of old Yunnan rice varieties, which belongs to the field of agricultural biotechnology, and more specifically belongs to the field of breeding. Background technique [0002] my country is one of the world-recognized origin centers of cultivated rice in Asia, one of the countries with the richest rice seed resources and one of the earliest rice-growing countries in the world. Due to its low latitude and large altitude difference (the lowest point is 76 meters above sea level and the highest point is 6,800 meters), Yunnan has obvious three-dimensional climate characteristics. Its geographical and climate diversity has bred rich and diverse rice seed resources. As the center of the richest diversity of ecological and genetic resources, it constitutes one of the most important gene banks of cultivated rice in Asia. [0003] At present, more than 6,000...

AI Technical Summary

Problems solved by technology

However, this method requires planting the materials in the field and doing a lot of field observations and records, which is time-consuming and labor-intensive, especially when faced with a large number of rice seed resources.

Moreover, it is difficult to distinguish some old varieties with indica and japonica characteristics with indica and japonica characteristics using the current method.

All in all, at present, there is no technology that can accurately, quickly and economically identify indica and japonica, an old variety of rice in Yunnan.

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