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Molecular marker for corn tripsacum monosome addition line nucleic male sterility genes and application thereof

A molecular marker, additional line technology, applied in application, plant genetic improvement, microbial assay/inspection, etc., can solve problems such as limited application, incomplete pollen abortion, and no discovery.

Active Publication Date: 2015-09-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem of incomplete pollen abortion in 200B limits the application of this material
[0005] After searching, there is no report on the use of molecular markers for genetic male sterility gene-assisted selection of monosomy addition lines in maize

Method used

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  • Molecular marker for corn tripsacum monosome addition line nucleic male sterility genes and application thereof
  • Molecular marker for corn tripsacum monosome addition line nucleic male sterility genes and application thereof
  • Molecular marker for corn tripsacum monosome addition line nucleic male sterility genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Screening Test of Molecular Markers of Monosomy Addition Line GMS Gene in Maize

[0077] Proceed as follows:

[0078] (1) In late March, 2012, at the Chengdu Campus of Sichuan Agricultural University, 10 seeds of Tripscaum and 50 seeds of Tripscaum monomer addition line 220B introduced from the US USDA were planted in germination boxes. The substrate is coarse river sand, placed in an artificial climate box with a temperature of 28°C and a humidity of 70% for germination. When the plants grow to three leaves and one heart, they are transplanted into plastic flower pots with a diameter of 30 cm and a height of 25 cm.

[0079] (2) When the corn friction grain additional line 220B plant is as high as 30cm, cut off the root tip of about 0.5cm at 10:00-14:00 at noon on a sunny day and when the temperature is higher than 25°C, and use saturated α-bromine Naphthalene aqueous solution was pretreated in the dark for 2 hours, and the pretreated root tips were hypotonic...

Embodiment 2

[0088]Example 2 Verification test of the molecular marker of the GMS gene in the monosomy addition line of maize friction grain

[0089] Proceed as follows:

[0090] In late March 2013, at the Chengdu Campus of Sichuan Agricultural University, the corn friction grain addition line (abbreviation: monomer addition line) MTchr2 (which was deposited in the China Microbiological Culture Collection Management Committee on July 18, 2014) was planted. Center, the preservation number is: CGMCC No.9552) 50 seeds (including monosomy addition seeds with 21 chromosomes and ordinary seeds with 20 chromosomes), when the plants grow to 5 to 6 leaves, single plants are listed. Genomic DNA of leaves was extracted, and PCR amplification was carried out using Mst1-F and Mst1-R as primers, and the specific method was the same as step (7) of Example 1. At the same time, the male fertility of the plants was identified during the flowering and pollination stage. Results The sterile strains (2n=20+1...

Embodiment 3

[0091] Example 3 Experiment of using the SCAR marker of the GMS gene of the monosomy addition line of maize to breed the male sterile line of maize

[0092] (1) Test materials:

[0093] (1), Maize Friction Grass monomer addition line (Zea mays) MTchr2 (preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on July 18, 2014, and the preservation number is: CGMCC No.9552)

[0094] (2) Maize inbred lines: B73, Zheng 58, Chang 7-2 (all provided by the Maize Institute of Sichuan Agricultural University).

[0095] (2) Test site:

[0096] Chengdu Campus of Sichuan Agricultural University and Xishuangbanna Experimental Base of Corn Research Institute of Sichuan Agricultural University

[0097] (3) Test method

[0098] (1) In late March 2013, at the Chengdu Campus of Sichuan Agricultural University, the additional line MTchr2 of corn friction grain monomer was obtained (it was deposited in the General Microorganism Center of Chi...

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Abstract

The invention discloses a molecular marker which is used for corn tripsacum monosome addition line nucleic male sterility genes and belongs to the field of crop molecular markers. The molecular marker is composed of nucleotide sequences shown by SEQ ID No:1. The invention further discloses an SCAR primer pair used for amplifying the molecular marker and composed of SEQ ID No:2 and SEQ ID No:3. In addition, the invention further discloses a method of using the molecular marker for backcross and recurrent selection breeding. The molecular marker is not limited by time and environment conditions, can identify and screen the corn tripsacum monosome addition line nucleic male sterility genes at the seedling stage, selects and weeds out the nucleic male sterility genes easily, saves manpower and material resources in large quantities, improves the breeding efficiency, shortens the breeding process and time, and substantially reduces breeding cost. In addition, the molecular marker can serve as a tool for large-scale recurrent selection for corns and continuously extend the corn heredity basis and is beneficial for selecting out a breakthrough corn variety.

Description

technical field [0001] The invention belongs to the field of crop molecular markers, and in particular relates to the molecular markers of the GMS gene of corn tribosome addition line and the application of the molecular markers of the GMS genes. Background technique [0002] Maize nuclear sterility, also known as genetic male sterility (Male Sterility, abbreviated as MS or ms), refers to male sterility controlled by nuclear genes. Since Eyster (Eyster L.A., Journal of Heredity, 1921.12:138-141) discovered the maize sterility gene in 1921, there have been 92 GMS genes reported so far (Sun Qingquan et al. Botany Bulletin. 2003.20 (2 ):248-2530). However, because of the segregation of fertility in the hybrid offspring of the genic male sterile gene, it is difficult to directly apply the maize sterility in maize production. [0003] Tripsacum (Tripsacum) is a close genus of Zea mays, which has the advantages of resistance to diseases and insect pests, high quality, and apomix...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68A01H1/02
Inventor 唐祈林王建银李志龙王红林李杨郑名敏马行云李晓峰周树峰吴元奇荣廷昭曹墨菊卢艳丽兰海
Owner SICHUAN AGRI UNIV
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