Method used for quickly breaking dormancy of peony seed epicotyl
A peony seed, high-efficiency technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as unsuccessful reports, achieve high phenotypic consistency, shorten the process of cultivation and genetic improvement, and improve efficiency Effect
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Embodiment 1
[0026] Epicotyl dormancy breaking method for soil germinated peony seeds:
[0027] (1) Select plump peony seeds, soak them in treatment solution I for 24 hours, discard the solution, keep the seeds, wash with warm water 3 times, air-dry, and put them in a 4°C refrigerator for later use;
[0028] (2) Take some clean river sand, sterilize at 121°C for 1 hour, cool to room temperature, soak the river sand with treatment solution II, bury the peony seeds treated in step (1) in the above wet sand, and keep it under dark conditions for 4 Stored at ℃ for 1 month, the roots appear and germination begins;
[0029] (3) Shallowly sow the rooted peony seeds in step (2) in a substrate with a mass ratio of peat, vegetable garden soil, and perlite at a ratio of 3:6:1, and grow one in a greenhouse at 28±2°C under natural light. month, petioles and leaves appear;
[0030] (4) Spray the leaves of the seedlings obtained in step (3) with the treatment liquid III, 7 days / time, and continue spray...
Embodiment 2
[0033] Epicotyl dormancy breaking method for culturing peony seed embryos in vitro:
[0034] (1) Select plump peony seeds, soak them in treatment solution I for 24 hours, discard the solution, keep the seeds, wash with warm water 3 times, air-dry, and put them in a refrigerator at 4°C or -20°C for later use;
[0035] (2) Peel off the seed shells of the peony seeds obtained in step (1), soak them in the treatment solution II for 24 hours, discard the solution, collect the seeds with endosperm, and absorb the surface moisture with filter paper;
[0036] (3) Wash the seeds with endosperm obtained in step (2) with tap water, blot the surface water droplets, disinfect with 70% volume concentration of ethanol for 30-90 seconds on the ultra-clean workbench, soak in 0.1wt% mercury chloride After 5-8 minutes, rinse with sterile water for 5-8 times, dry the water drops on the surface of the sterile material with sterile filter paper, peel off the seed embryos, and inoculate them in WPM ...
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