A method for high-efficiency in vitro regeneration of larch needles

Abstract

The invention discloses a method for high-efficiency in vitro regeneration of larch needles. The larch needles are used as a starting material to produce a large number of adventitious buds after cultivation, and a large number of larch regenerated plants can be obtained in a short time. The plant phenotype The consistency is high, and the occurrence of this seedling is not limited by seasons. The regeneration system can be used as a transgene recipient system to further prepare transgenic plants, so that they can not only maintain the genetic phenotype of the parents, but also have the traits of the transferred exogenous target gene. By establishing an in vitro regeneration method with larch needles as the starting explants, research on the genetic improvement of conifers represented by larch, the cultivation of new varieties (lines) and the propagation of high-quality seedlings will contribute to the sustainable development of forestry economy. It is of far-reaching significance to narrow the gap between the field of forestry basic research and developed countries.

Description

technical field [0001] The invention relates to the field of plant regeneration and rapid technology, in particular to the in vitro regeneration of larch. Background technique [0002] Larix Mill (Larix Mill) is a deciduous tree belonging to the genus Larix of the Pinaceae family. It is widely distributed, grows quickly, has strong adaptability, and has few pests and diseases. It is valued and widely used in forestry production. At the same time, larch wood has the advantages of long fiber, decay resistance, compression resistance and bending resistance, so larch can not only be used as a high-quality building material, but also as a basic raw material for high-grade printing paper. There are 18 species of larch, which are mainly distributed in temperate mountainous areas, cold temperate plains and alpine climate zones in the northern hemisphere. There are 10 species in my country, which are mainly distributed in Northeast China, Xiaoxing'an Mountains, North China, Northwes...

AI Technical Summary

Problems solved by technology

[0004] The current research mainly focuses on zygotic embryos or hypocotyls as explants, whose source is easily limited, and the regeneration effect needs to be improved, and the practicability is not strong enough to be widely used.

It can be seen that there is still no effective regeneration system found, which has always been a difficult problem to be overcome.

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