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Co-separation molecular marker TCR540 of clubroot resistant CRb gene of Chinese cabbages, primers and application

An anti-clubroot, molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve problems such as chain burden, and achieve the effect of shortening the breeding process and improving the breeding efficiency.

Inactive Publication Date: 2016-10-12
SHENYANG AGRI UNIV
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Problems solved by technology

However, these markers are not markers that co-segregate with CRb, and the use of these markers for molecular marker-assisted breeding is likely to cause linkage drag

Method used

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  • Co-separation molecular marker TCR540 of clubroot resistant CRb gene of Chinese cabbages, primers and application
  • Co-separation molecular marker TCR540 of clubroot resistant CRb gene of Chinese cabbages, primers and application
  • Co-separation molecular marker TCR540 of clubroot resistant CRb gene of Chinese cabbages, primers and application

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Experimental program
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Embodiment Construction

[0027] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0028] 1. Acquisition of co-segregation molecular markers TCR540 and TCR541 of Chinese cabbage clubroot resistance CRb gene

[0029] 1. Construction of isolated groups

[0030] F 1 , the obtained F 1 All the offspring plants showed resistance to the disease. The above two parents 'CRBJN3-2' and 'BJN3-2' are preserved in Shenyang Agricultural University. Select individual plant F 1 selfing build F 2 There are 4,783 groups in the mapping group. All 4783 Fs Seeded 2 Groups were inoculated with Plasmodium 10 days after planting. After 40 days for disease resistance identification, 4783 F 2 In the population, 3641 were resistant and 1142 were susceptible, and the chi-square test met the segregation ratio of 3:1

[0031] 2. DNA ex...

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Abstract

The invention discloses a co-separation molecular marker TCR540 of a clubroot resistant CRb gene of Chinese cabbages. A nucleotide sequence of the co-separation molecular marker TCR540 is represented as SEQ ID NO.1, TCR540 and the CRb gene are co-separated, and TCR540 can be applied to molecular marker assisted selection of the clubroot resistant CRb genes of Brassica including Chinese heading cabbages and green pakchoi. The markers are used simultaneously in an assisted selection process, the theoretical accuracy of the selection can be up to 100%, and the co-separation molecular marker TCR540 is good in repeatability, high in reliability and low in detection cost and saves time and labor.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a co-separation molecular marker TCR540 of Chinese cabbage clubroot resistance CRb gene, primers and applications. Background technique [0002] In 2002, our research group obtained a Chinese cabbage double haploid line 'CR Shinki DH' resistant to brassica clubroot, and the research showed that the disease-resistant material was resistant to physiological races 2, 4 and 8 of Plasmodium brassicae (Piao et al., Conversion of AFLP marker linked to clubroot resistance gene into SCAR marker. 2002, J Kor Soc Hort Sci 43:653-665). Further studies have proved that the clubroot resistance gene is a dominant single gene and named as CRb (Piao et al., SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp.pekinensis), Theor Appl Genet, 2004, 108:1458-1465). [0003] During the transformation of brassica species ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13
Inventor 朴钟云张腾庞文星李晓楠朴英兰张椿雨
Owner SHENYANG AGRI UNIV
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