SNP marker related to growth traits of black-bone chickens and application thereof
A marker and polymorphism technology, which is applied in the determination/testing of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of high breeding cost, inaccurate selection, long breeding process, etc.
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Embodiment 1
[0044] Example 1. Verification of the correlation between the SNP marker rs317682933 and the body weight and feed intake of Lueyang black-bone chicken
[0045] 1. Test animals
[0046] Lueyang black-bone chickens are randomly selected from three generations of Lueyang black-bone egg breeding core group bred by Lueyang Longjia Agricultural Science and Technology Development Co., Ltd. All Lueyang black-bone chickens are hatched in the same batch and raised in the same chicken house. 40pcs / m 2Cage brooding; brooding temperature is 33-35°C in the first week, then lowered by 2-3°C every week, and removed from the temperature in the fourth week; the light time is 22h in the first week, and then reduced by 1-1.5h every week, using Huaqin 911A egg chicks were fed with compound feed. In 60 days, they were transferred to the laying hen house and raised in individual single cages.
[0047] 2. Determination of body weight and material consumption
[0048] Measure the body weight of bl...
Embodiment 2
[0081] Embodiment 2, the comparison of the breeding effect of traditional phenotype selection and marker-assisted selection
[0082] 1. Test animals
[0083] 500 hens and 200 roosters were randomly selected from the second generation of egg-line breeding core group raised by Lueyang Longjia Agricultural Science and Technology Development Co., Ltd. to form a traditional phenotype selection breeding group, and another 500 hens and 200 roosters were selected SNP marker-assisted selection breeding population.
[0084] 2. Test plan
[0085] Traditional phenotype selection is based on 12-week-old body weight, and individual phenotype selection is adopted. The top 5% of cocks are sorted by 12-week-old body weight, and the top 5% of hens are reserved for 25%. The marker-assisted breeding method not only considers the body weight at 12 weeks, but also considers the genotype of the rs317682933 locus, and the proportion of reserved seeds is the same as that of the traditional breeding ...
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