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199 results about "Gene specific primer" patented technology
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Gene Specific primers enhance sensitivity by directing all of the RT activity to a specific message instead of transcribing everything in the mix. If you are performing a one-step RT-PCR, gene specific primers are used because the RT primer is also your reverse primer for the PCR step.
Nucleic acid assays employing universal arrays
InactiveUS20010026919A1Microbiological testing/measurementBiological testingGene expressionNucleic acid
Hybridization assays, as well as kits, primers and arrays for use in practicing the same, are provided. In the subject assays, a population of tagged target nucleic acids generated from a population of tagged gene specific primers is contacted with an array of tag complements under hybridization conditions and the presence of any resultant hybridized tag target nucleic acid-tag complement structures is detected. The subject arrays find use in a number of different applications, e.g. differential gene expression analysis.
Owner:CLONTECH LAB
Methods and compositions for analyzing AHASL genes
The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and / or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
Owner:BASF AG
Endpoint taqman methods for determining zygosity of corn comprising tc1507 events
InactiveUS20110151441A1High throughput zygosity analysisSugar derivativesMicrobiological testing/measurementReference genesPcr assay
A method for zygosity analysis of the maize Cry1F event TC1507 is provided. The method provides TC1507 event-specific and maize endogenous reference gene-specific primers and TaqMan probe combinations for use in an endpoint biplex TaqMan PCR assay capable of producing robust genotype calls for assisting in molecular breeding of TC1507.
Owner:DOW AGROSCIENCES LLC
Method and kit for detecting lung cancer susceptibility gene
ActiveCN102373287AHigh detection sensitivityMicrobiological testing/measurementCell sensitivityLung cancer susceptibility
The invention relates to the field of genetic engineering, and provides a method and a corresponding kit for detecting a lung cancer susceptibility gene. The method for detecting the lung cancer susceptibility gene comprises the following steps of: A, using a specificity primer of the lung cancer susceptibility gene to amplify a plurality of target areas in a sample to be detected, and establishing a sequencing library based on amplified products; B, subjecting the sequencing library to monomolecular amplification to obtain a plurality of monomolecular amplification products corresponding to the plurality of target areas; and C, subjecting the plurality of monomolecular amplification products to high-throughput gene sequencing at the same time so as to obtain sequence information of the plurality of target areas. The method and the corresponding kit are used for sequencing the plurality of areas of the lung cancer susceptibility gene at the same time by using a high-throughput sequencing technology; the sequence information including variation of known mutations and unknown mutations in the areas can be obtained accurately; the kit has high detection sensitivity, and can be used for further detecting mainly samples at the same time.
Owner:盛司潼
Population scale HLA-typing and uses thereof
InactiveUS20070298425A1Bioreactor/fermenter combinationsBiological substance pretreatmentsGene specific primerPopulation
The present invention provides a portable system for real-time population-scale HLA genotyping and / or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
Owner:GENOMICS USA
Compositions and Methods for RT-PCR
ActiveUS20140199699A1Rapid and efficient amplificationHigh detection sensitivityMicrobiological testing/measurementTrehaloseBiology
The present invention relates to methods and compositions having trehalose and DNA polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules, and for increasing the detection sensitivity and reliability through generation of secure cDNA molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) trehalose in a concentration between about 5% and about 35%; (b) a viral reverse transcriptase; and (c) at least one DNA polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates.
Owner:LEE JUN EUIHUM
Primer group for simultaneously identifying wild strain and gene deletion strain of African swine fever based on multiple qPCR technology and test kit
InactiveCN111996191AReduce pollutionShorten the timeMicrobiological testing/measurementDNA/RNA fragmentationAfrican swine feverViral test
The invention provides a primer group for simultaneously identifying a wild strain and a gene deletion strain of African swine fever based on a multiple qPCR technology and a test kit, and belongs tothe technical field of virus detection. The primer probe group for simultaneously identifying the wild strain and the gene deletion strain of the African swine fever based on the multiple qPCR technology comprises a p72 gene specific primer probe, a CD2v gene specific primer probe and an MGF gene specific primer probe. The test kit comprises the primer probe group. Multiple qPCR detection is performed by adopting the test kit; p72 gene specific primers of a conserved gene of ASFV can amplify the wild strain and the gene deletion vaccine strain; specific primers of a CD2v gene and an MGF gene can only amplify the wild strain; and the three pairs of primers are combined for use, so that the wild strain and the gene deletion vaccine strain of the ASFV can be simultaneously identified. The primer group and the test kit have the advantages of accurate detection, sensitivity, high efficiency, low cost and the like, and have relatively high practical value for diagnosis of clinical samples and the breeding industry.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
Selective amplification of overlapping amplicons
The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag t3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.
Owner:PILLAR BIOSCI INC
Method of detecting one or more limited copy targets
InactiveUS20080050724A1Facilitate conductionEliminate needMicrobiological testing/measurementGeneNon specific
A method allowing simultaneous amplification of multiple low-abundance targets in environmental samples. This is a two-step process that includes a combined reverse transcription and pre-amplification step, which utilizes a mix of gene-specific primer sets, followed by a second amplification step performed on the previously generated “RT-amplification” product. Initial amplification of each target is performed prior to the splitting of the sample for individual amplification and identification. The method combines the process of reverse transcription and amplification within a single processing apparatus. The method also enables gene-specific reverse transcription using gene-specific primers, thereby reducing if not eliminating non-specific product in this reverse transcription step of the process.
Owner:MICROFLUIDIC SYST
Messenger RNA profiling: body fluid identification using multiplex reverse transcription-polymerase chain reaction (RT-PCR)
InactiveUS7270983B1Strong specificityImprove timelinessSugar derivativesMicrobiological testing/measurementSemenStain
This invention relates to a body fluids identification method and kit. A parallel, multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the definitive identification of body fluids commonly encountered in forensic casework analysis, namely blood, saliva, semen, and vaginal secretions. The methodology is based on gene expression profiling analysis in which the body fluid-specific genes are identified by detecting the presence of appropriate messenger RNA species. Gene-specific primers are labeled with fluorescent dyes, separated and subjected to laser induced fluorescence for identification of body fluid-specific genes present in a sample stain.
Owner:UNIV OF CENT FLORIDA RES FOUND INC
Reference-containing high-sensitivity fluorescent quantitative polymerase chain reaction (PCR) kit for Epstein-Barr virus
ActiveCN103642945AHigh detection sensitivityAvoid misdiagnosisMicrobiological testing/measurementFluoProbesViral nucleic acid
The invention relates to a kit for quantitative detection of an Epstein-Barr virus (EBV) nucleic acid by using a fluorescent polymerase chain reaction (PCR) technology. A pair of primers is used for amplifying an EBV specific nucleic acid sequence and EBVDNA is quantitatively detected through a fluorescence probe and meanwhile, an internal reference DNA is detected by using a human endogenous gene specificity primer and the fluorescence probe. By adopting the kit, existence of the EBV and internal reference nucleic acid is simultaneously detected by a single-tube double-wavelength fluorescent PCR technology, the EBVDNA in whole blood, plasma and a nasopharyngeal secretion sample can be quantitatively detected, and the whether a PCR inhibitor or template loss caused by a misoperation in detection exists in the sample is judged by the detection result of the internal reference nucleic acid. Thus, the kit is simple, convenient and fast in operation, can provide sensitive and accurate quantitative result, and can be widely applied to quantitative detection of clinical EBV virus infection.
Owner:SHANGHAI XINGYAO MED TECH DEV CO LTD +1
PCR method for quickly detecting brucella in milk sample
InactiveCN101659995APracticalHigh sensitivityMicrobiological testing/measurementMicroorganism based processesMilk samplePcr method
The invention discloses a PCR method for quickly detecting brucella in a milk sample, relating to a gene detection technology of pathogens of zoonotic infectious diseases and being applicable to qualitative detection of brucella. The PCR method consists of the components of a standard positive template, a PCR reaction solution, a brucella BCSP31 gene specific primer and a negative quality-controlstandard sample. The invention has the advantages of quick detection speed, good specificity, high sensitiveness, simple using steps, high repeatability, and capability of replacing the traditional pathogenic detection method.
Owner:JILIN UNIV
Genetic diagnosis kit for amyotrophic lateral sclerosis
ActiveCN103898211AGuaranteed speedGuaranteed accuracyMicrobiological testing/measurementSequence analysisProtide
The invention discloses a genetic genetic diagnosis kit for amyotrophic lateral sclerosis. The kit and a detection method are characterized in that gene-specific primers are designed and synthesized according to a virulence gene which is studied and reported to be correlated with the amyotrophic lateral sclerosis, including SOD1, FUS, VAPB, ANG, TARDBP, FIG4, OPTN, VCP, Sigmar1, CHMP2B and PFN1 sites, a sample is obtained from an individual to be detected, a RT-PCR technology is carried out, the RT-PCR product is subjected to sequencing analysis, next, the sample sequence is compared with a normal gene sequence to determine whether the sample sequence has disease-causing mutations. The kit is capable of detecting all disease-causing mutation sites of the coding sequence (CDS) of the virulence gene at a time, and has the advantages of being simple and quick, reliable in preparation, good in specificity, high in sensitivity and the like; the kit is capable of detecting the ALS patients and asymptomatic patients.
Owner:江苏雄鸣医药科技有限公司
Fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Leishmania donovani
InactiveCN101613752AQuantitatively accurateThe detection process is fastMicrobiological testing/measurementMicroorganism based processesEtiologyBiology
The invention discloses a fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Leishmania donovani, relating to gene detection technology of zoonosis pathogen, and being applicable to qualitative and quantitative detection of Leishmania donovani. The invention comprises a standard positive template, a fluorescent quantitative PCR reaction solution, Leishmania donovani kDNA gene specific primer and negative quality control standard product. The invention is accurate in quantifying, rapid in detection speed, good in specificity, high in sensitivity, simple in use steps and high in repeatability and can replace traditional etiology detection method.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Primer, kit and method for quickly detecting HLA-B*5801 allele
ActiveCN104017898AImprove bindingAvoid it happening againMicrobiological testing/measurementDNA/RNA fragmentationHLA-BBiology
The invention belongs to the technical field of gene engineering, and discloses a primer for quickly detecting HLA-B*5801 allele, a kit containing the primers and a method for quickly detecting HLA-B*5801 allele by using the primers and kit. By using the HLA-B*5801 gene specific primer and adopting the multiplex primer combination design, the SNP (single-nucleotide polymorphism) site of the HLA-B*5801 gene is completely covered, the specific combination is enhanced, and the generation of the false positive is prevented more effectively. Besides, the specific primer, dNTPs (deoxyribonucleotide triphosphates), PCR (polymerase chain reaction) buffer solution and dyes are mixed previously, thereby greatly saving the operation time and workload; and the method is quick, simple, accurate and visual, and can the screening and typing experiment of the whole gene within 3 hours, thereby solving the problem of safe application instructions of the HLA-B*5801 gene in drugs for treating gout and the like.
Owner:SHANGHAI TISSUEBANK BIOTECH +3
Beta-mannase gene and amino acid sequence of its coded product and preparation method
InactiveCN1807644AExpand genetic resourcesEnrich excellent candidate genesHydrolasesRecombinant DNA-technologyBiotechnologyBacterial strain
The invention discloses an amino acid sequence of beta-mannase gene and coding making method in the biological domain, which is characterized by the following: extracting Armillariella tabescens main RNA of high activity by konjaku flour evoking; using consanguinity sequence of Genebank to design degenerate primer; carrying on PCR expanding to protectorate by primer; designing gene specificity primer to carry on 3'RACE and 5'RACE; cloning total long cDNA of beta-mannase gene; constructing eucaryon expressing carrier with pichia; building-up yeast gene engineering bacterial strain.
Owner:JINAN UNIVERSITY
Method and reagent for detecting transgenic maize strain VCO-01981-5
The invention discloses a method and a reagent for detecting the transgenic maize strain VCO-01981-5. The detection method provided by the invention comprises the following steps: adding mineral oil to a PCR reaction system, carrying out uniform mixing, transferring the mixture to a droplet generator for generating droplets, carrying out PCR reaction, after the reaction, reading an amplification signal of each droplet, and calculating the target DNA molecule number of a to-be-detected sample by adopting QuantaSoft software, wherein the PCR reaction system comprises strain VCO-01981-5 and HMG gene specific primers and probes, and the two probes are subjected to FAM and HEM fluorescence labeling. According to the quantitative determination method provided by the invention, the endogenous gene HMG and strain specific primers and probes matched for use are designed, by adopting a double-channel method, the molecule numbers of the endogenous gene HMG and the strain VCO-01981-5 in a maize gene group are quantified, the absolute content of the transgenic maize strain VCO-01981-5 is calculated, and thus the detection method has great significance for the research for the transgenic ingredients and the transgenic detection at the ports of china.
Owner:SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT
Molecular identification method for zero-type fruit branch genes of cotton
ActiveCN104561284ASpeed up breedingImprove convenienceMicrobiological testing/measurementBiotechnologyMolecular identification
The invention belongs to the field of biotechnology, relates to a molecular identification method for zero-type fruit branches of cotton, and particularly provides a gene specific primer marker used for identifying a cotton strain with zero-type fruit branch genes. Nucleotide sequences are as shown in SEQ ID No.1 and SEQ ID No.2. An individual strain carrying the zero-type fruit branch genes in a segregation population is identified by the gen specific marker. The method is high in identification speed, high in accuracy, simple to carry out and very suitable for indoor identification and molecular marker-assisted selection of the zero-type branches of the cotton.
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Primers, kit and detection method for detecting gene type of dominant white feather site of chicken PMEL17 gene
ActiveCN103602745AAccurate detectionQuick checkMicrobiological testing/measurementDNA/RNA fragmentationDominant whiteGene type
Owner:HENAN AGRICULTURAL UNIVERSITY
Method for quantitating anaerobic ammonia oxidizing bacteria in sediment of aquiculture environment
InactiveCN102925581ACreate Quantitative ResearchOvercome the disadvantages of difficult isolation and cultureMicrobiological testing/measurementFluorescence/phosphorescenceBacteroidesCoverage ratio
The invention relates to a method for quantitating ammonia oxidizing bacteria in a sediment of an aquiculture environment by a real-time fluorescent quantitative PCR (polymerase chain reaction) technology, which belongs to the field of environmental microbial ecology. The method comprises the steps that DNA (deoxyribonucleic acid) of a sample sediment is extracted and quantitated; an HZO (hydrazine oxidordeuctase) gene specific primer is subjected to PCR amplification; standard plasmids are constructed; and a fluorescent quantitation PCR amplification curve and a standard curve are made. The quantity of the anaerobic ammonia oxidizing bacteria in a sample is calculated by comparing a detected sample Ct value with the standard curve; an obtained log value of the initial template gene copy number has a better linear relationship with the Ct value; and a regression coefficient is greater than 0.99. According to the method, an HZO gene is used for conducting quantitative analysis on the ammonia oxidizing bacteria under an anaerobic condition; the deficiencies that the specificity is not high, the coverage ratio is low and the method is inaccurate due to the fact that the a 16S rRNA (ribosomal ribonucleic acid) gene is used for quantitating in the existing method are overcome; and the method for quantitating the ammonia oxidizing bacteria in the sediment of the aquiculture environment is high in specificity, quick and accurate.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)
Fluorescence quantitative RT-PCR detection reagent for PPR virus and preparation method and use thereof
InactiveCN1840700AConvenient and efficientImprove featuresMicrobiological testing/measurementFluorescence/phosphorescenceFluorescenceGene
The related bio-reagent is designed by: with conservative PPR (PESTE DES PETITS RUMINANTS), virus H gene fragment as target object, designing and composing carefully for large quantities of primers and probes, taking optimal pairing screen test on different conditions to obtain the most proper primer and probe. The fluorescence quantitative RT-PCR testing reagent includes one couple of specific primers and one specific fluorescent probe, and the amplification fragment length is 76bp.
Owner:CHECKOUT & QUARANTINE TECH CENT YUNNAN ENTRY &EXIT CHECKOUT & QUARANTINE BUR
Multiplex Preparation of Barcoded Gene Specific DNA Fragments
ActiveUS20200063190A1Identification and elimination of PCR duplicate biasesReduce complexityMicrobiological testing/measurementDNA preparationForward primerMultiplex
Methods of preparing a plurality of sample-barcoded anchor-domain-flanked gene specific deoxyribonucleic acid (DNA) fragments from a template nucleic acid, e.g., ribonucleic acid (RNA), sample are provided. Aspects of the methods include employing a set of gene specific primer pairs, wherein each pair of gene specific primers is made up of a forward primer and a reverse primer, at least one of which includes a sample barcode domain. The methods find use in a variety of different applications, including high-throughput sequencing, e.g., expression profiling, applications, including of small biological samples, e.g., single-cells.
Owner:CELLECTA
KASP marker typing primer combination for high-throughput detection of mutation sites of AhFAD2B gene and application of primer combination
InactiveCN109593876AEfficient identificationImprove throughputMicrobiological testing/measurementDNA/RNA fragmentationNucleotideGene type
The invention discloses a KASP marker typing primer combination for high-throughput detection of mutation sites of an AhFAD2B gene and an application of the primer combination, belongs to the technical field of molecular genetic breeding and relates to a special primer combination for detecting types of the AhFAD2B gene of high-oleic-acid peanuts by a high-throughput SNP molecular marker and the application of the primer combination. The primer combination is the KASP marker typing primer combination for detecting C>T mutant sites at 814 sites of the AhFAD2B gene of high-oleic-acid peanuts andcomprises an A-labeled wild type AhFAD2B gene specific primer with a nucleotide sequence shown in SEQ ID No.1, a B-labeled mutant AhFAD2B gene specific primer with a nucleotide sequence shown in SEQID No.2 as well as a AhFAD2B gene general primer with a nucleotide sequence shown in SEQ ID No.3. The high-throughput KASP molecular marker gene typing specific primers are designed for novel mutant 814 C>T allelic variation of the AhFAD2B of high-oleic-acid peanuts, novel mutants of the AhFAD2B can be effectively identified, and the problem that identifying methods in the prior art can only identify 448 G>A allelic variation of classic F435 mutant sites of the AhFAD2B is solved.
Owner:INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
N gene specific primer pair, method and kit for detecting resistance of tobacco to TMV as well as kit
ActiveCN103866038AHigh amplification efficiencyGuaranteed specificityMicrobiological testing/measurementDNA/RNA fragmentationNicotiana tabacumAgricultural science
The invention provides an N gene specific primer pair, a method and a kit for detecting resistance of tobacco to a TMV. The specific primer pair comprises an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is as shown in SEQ ID NO:1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO:2. The method comprises the following steps: designing a pair of specific primers in a tobacco N gene sequence specific zone; with the DNA of a to-be-detected tobacco variety as a template, performing PCR amplification on the N gene specific primer pair; and detecting a PCR amplification product by electrophoresis and judging whether the PCR amplification product contains a 865bp characteristic band, wherein if so, the tobacco has resistance to TMV, and otherwise, the tobacco does not have N gene mediated resistance to TMV. The method has the advantages of reliable result, high detection speed and the like and is simple to operate, the breeding process of TMV-resisting breeding materials can be greatly accelerated, the breeding period is shortened and the breeding efficiency is enhanced.
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD
Discrimination method of target base in DNA, and allele specific primer used in the method of the same
ActiveUS20070117118A1Low efficiencyResolution problemMicrobiological testing/measurementFermentationCancer researchBase sequence
An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-TT-3′, 5′-GA-3′ or 5′-GT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
Owner:PANASONIC CORP
Gene expression amount comparing analysis method
InactiveCN1398988AHigh sensitivityGood quantitative effectMicrobiological testing/measurementDiseaseQuantitative determination
An analytical method for quantitative gene expression amount determination and comparison has great significance for disease-related gene screening and in clinical early diagnosis and development of specific medicine. The present invention utilizes quantitative characteristic of biological luminance process is sequencing to compare the gene expression amount of the same gene in different individuals or samples and to search disease-related gene. The specific steps includes: making mRNA with different sources by using a proper method and mixing in equal amount to form PCR substrate; PCR amplification with the primer corresponding to the gene of different sources and gene specific primer; and determining sequence by using bioluminescence analysis process with base variety to replace gene of different source.
Owner:周国华 +1
Quantitative measuring transgene component in transgene rapeseed and processed product
A process and reagent kit for quantitatively detecting the transgenic component content in transgenic rape seed and its products are disclosed. Said process includes extracting DNA, PCR amplification by use of extracted DNA as template, PEP-specific primer pair and exogenous gene specific primer pair, using the amplified resultants to respectively hybridize with the first and the second probes, detecting the detectable signals generated by hybridization, and analyzing the detected data. Its advantages are high correctness and simple operation.
Owner:SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
Fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Toxoplasma gondii
InactiveCN101613751AQuantitatively accurateThe detection process is fastMicrobiological testing/measurementMicroorganism based processesEtiologyBiology
The invention discloses a fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Toxoplasma gondii, relating to gene detection technology of zoonosis pathogen, and being applicable to qualitative and quantitative detection of Toxoplasma gondii. The invention comprises DNA extracting solution, a standard positive template, a fluorescent quantitative PCR reaction solution, Toxoplasma gondii B1 gene specific primer and a negative quality control standard product. The invention is accurate in quantifying, rapid in detection speed, good in specificity, high in sensitivity, simple in use steps and high in repeatability and can replace traditional etiology detection method.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Genetic diagnosis kit of Alzheimer's disease
PendingCN105969885AEasy to detectGuaranteed speedMicrobiological testing/measurementDiseaseSequence analysis
The invention discloses a genetic diagnosis kit of Alzheimer's disease. The kit can detect, research and report 10 virulence genes related to Alzheimer's disease, including APP, APOE, PSEN1, PSEN2, GSK3beta, DYRK1A, Tomm40, CLU, PICALM and TAU. A detecting method comprises the following steps: designing and synthesizing specific primers of all genes, collecting the sample of a individual to be detected, adopting the RT-PCR technology to carry out sequencing analysis on obtained specificity DNA products of all genes, comparing the sample sequence with the normal gene sequence, and analyzing whether pathogenic mutations exist to evaluate the morbidity risk of the individual. The genetic diagnosis kit of Alzheimer's disease can detect different pathogenic mutation sites of CDS at a time and has the advantages of high simpleness, convenience, rapidity and sensitivity, reliable preparation and excellent specificity.
Owner:江苏雄鸣医药科技有限公司
Discrimination method of target base in DNA, and allele specific primer used in the method of the same
ActiveUS20070042393A1Low efficiencyResolution problemMicrobiological testing/measurementFermentationCancer researchBase sequence
An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is T. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of T, C or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
Owner:PANASONIC CORP
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