Separation and determination method for mobility of nicotine optical isomers in cigarettes
A technology for optical isomers and determination methods, which is applied in the field of tobacco analysis, can solve the problems of large influence on the stability of the method, insufficient sensitivity, and long analysis time of two-dimensional gas chromatography, so as to solve the interference of complex matrix and quantitative determination accurate effect
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[0033] The content of the present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.
[0034] (1) Instruments and reagents
[0035] Agilent1260-Agilent1290 two-dimensional liquid chromatograph (Agilent Company of the United States); Milli-Q-Intergral5 ultrapure water instrument (Millipore Company of the United States); SHZ-88 water bath constant temperature oscillator (Jintan Medical Instrument Factory); BT125D electronic balance ( Sensitivity 0.0001g, German Sartorius Scientific Instrument Co., Ltd.); KQ-700DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); centrifuge tube with stopper, 50mL; disposable sterile syringe; 5mL; organic phase filter membrane , 0.22 μm (Shanghai Chuding Analytical Instrument Co., Ltd.).
[0036] Methanol (chromatographically pure, Merck Company of Germany), acetonitrile (chromatographically pure, Merck Company of Germany), triethyl...
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