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Rheumatosis autoantibody immunoblotting reagent kit

An autoantibody, immunoblotting technology, applied in material analysis, measuring device, instrument, etc. by observing the effect on chemical indicator, can solve the problem of no simultaneous detection, etc.

Inactive Publication Date: 2007-08-22
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there is no simultaneous detection of 8 kinds of autoantibodies (Sm, U1RNP, ssA, ssB, Scl-70, Jo-1, Ro60, Rib antibodies), systemic lupus erythematosus (SLE), Sjogren's syndrome (SS), hard Western blot kit for the diagnosis and differential diagnosis of 6 rheumatic diseases including dermatosis (PPS), dermatomyositis and polymyositis (DM / PM), mixed connective tissue disease (MCTD), and rheumatoid arthritis (RA)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] The components of this kit include: 5 plates of blotted membrane strips placed in the reaction tank, 8 strips per plate, 0.5ml of enzyme-linked reagent, 25ml of chromogenic reagent A, 25ml of chromogenic reagent B, 25ml of stop solution, 25ml of concentrated washing solution, standard zone . Sample requirements: Take 2-5ml of blood from the vein, put it in a test tube and centrifuge the serum after coagulation, store the serum sample at 2-8°C, and store the sample that cannot be tested within 24 hours at -20°C, severe hemolysis, flocculent Or moldy serum samples will affect the test.

[0009] Measurement steps

[0010] 1. Preparation of washing application solution

[0011] The concentrated washing solution stored at 2-8°C will crystallize. Dilute the whole bottle (25ml) of the concentrated washing solution to 500ml with distilled water or deionized water, put it into a reagent bottle, and indicate the preparation time on the label, and store it at 2 -8 ℃, valid for ...

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PUM

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Abstract

The invention discloses a immune imprinting regent box of the rheumatism self antibody which includes the print film in the reacting slot, the enzyme linked regent, the color regent A liquid, the color regent B liquid, the stop liquid, the condense washing solution and the standard band. Firstly, the ENA is divided by the SDS-polyacrylamide gel electrophoresis according to the molecular weight, then transfers it into the print membrane by the print technology which includes the ENA arrayed by the molecular weight; if the detected blood serum has the self antibody, it will disappear the color band by adding the enzyme linked and the color regent after combining with the according antigen. So it can detect that if the serum has the antibody of Sm, U1RNP,ssA,ssB,Scl-70,Jo-1,Ro60,Rib to diagnose and identify the rheumatism.

Description

technical field [0001] The invention relates to a rheumatism autoantibody immunoblotting kit. Background technique [0002] At present, there is no simultaneous detection of 8 kinds of autoantibodies (Sm, U1RNP, ssA, ssB, Scl-70, Jo-1, Ro60, Rib antibodies), systemic lupus erythematosus (SLE), Sjogren's syndrome (SS), hard Western blot kit for the diagnosis and differential diagnosis of 6 rheumatic diseases including dermatosis (PPS), dermatomyositis and polymyositis (DM / PM), mixed connective tissue disease (MCTD), and rheumatoid arthritis (RA) . Contents of the invention [0003] The object of the present invention is to provide a kind of rheumatism autoantibody immunoblotting kit, utilize immunoblotting technique, can detect 8 kinds of autoantibodies (Sm, U1RNP, ssA, ssB, Scl-70, Jo-1 simultaneously) on a blot film , Ro60, Rib antibody), providing reliable laboratory basis for the diagnosis and differential diagnosis of rheumatic diseases. [0004] In order to achieve...

Claims

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Application Information

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IPC IPC(8): G01N33/559G01N21/78
Inventor 马伟民张永顶
Owner SHENZHEN BLOT BIOTECH
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