Immune preparation for treating tumor and preparation method thereof

An immunological preparation and tumor technology, applied in the field of biomedicine, can solve the problems of low efficacy and poor targeting of non-specific cellular immunotherapy, and achieve the effects of enhanced sensitivity, good clinical efficacy, and disappearance of varicose veins.

Inactive Publication Date: 2016-08-10
SHANGHAI PRECISION DIAGNOSTICS CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0018] Aiming at the above-mentioned technical problems in the prior art, the present invention provides an immune preparation for treating tumors and a preparation method thereof, and the immune preparation for treating tumors and a preparation method thereof shall solve the problems widely encountered in the prior art. The non-specific cellular immunotherapy used is not highly targeted and has low efficacy technical problems

Method used

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  • Immune preparation for treating tumor and preparation method thereof
  • Immune preparation for treating tumor and preparation method thereof
  • Immune preparation for treating tumor and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0069] Operation steps of tumor-targeted DC-CIK immunotherapy:

[0070] 1) Collection of peripheral blood mononuclear cells (PBMC):

[0071]Take 50ml of blood from the patient's vein, use a blood cell separator to separate and enrich PBMC, and perform preliminary purification by density gradient centrifugation of Ficoll lymphocyte separation medium. The upper plasma was placed in another centrifuge tube, inactivated at 56°C for 30 minutes, and stored at 4°C for later use. Slowly absorb the cloud layer (that is, the mononuclear cell layer) with a dropper, add 0.9% physiological saline to wash twice, and centrifuge at 1500 rpm for 5-10 minutes.

[0072] 2) DC and CIK cell culture

[0073] Use LonZA X-VIVO 15 serum-free medium (10% human serum albumin) to adjust the cell concentration to 6×10 6 / ml, 2ml per well. Placed at 37°C, 5% CO 2 After incubating in the incubator for 2 hours, the non-adherent cells were CIK precursor cells, and the CIK culture medium was used to press...

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Abstract

The invention discloses an immune preparation for treating tumor; the immune preparation contains dendritic cells (DC) loaded with a tumor antigen and cytokine-induced killer cells (CIK), the concentration of the DC loaded with the tumor antigen in a culture liquid is 1*10<7> to 1*10<8>, and the concentration of the CIK in the culture liquid is 1*10<9> to 1*10<10>. The invention further provides a preparing method of the immune preparation for treating tumor; the preparing method comprises the steps: collection of mononuclear cells of peripheral blood; isolation and culture of the DC and the CIK; extraction of a tumor antigen gene and viral vector packaging; transfection of the DC with a virus mediated tumor antigen gene; and co-culture of the DC loaded with the tumor antigen and the CIK. The accuracy of positioning of re-transported immune cells in a human body can be improved. The objective defect of a conventional cell immunotherapy technology in the same field is not good in solid tumor treatment effect can be effectively improved; adaptation diseases are wide, and the use is safe and effective.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to an immune preparation for treating tumors and a preparation method thereof. Background technique [0002] Cancer is a major disease that seriously threatens human life and social development, and has become an important cause of human death. In 2015, 9 million people were estimated to die from cancer in many countries, and this number will reach 11.4 million in 2030. Cancer is a major disease that seriously threatens human life and has become an important cause of human death. According to the data in the 2015 Cancer Registration Annual Report released by the National Cancer Registry Center, there were about 3.37 million new cases of malignant tumors in China in 2011, and about 2.11 million people died of them. Due to its large population base, China has the highest number of cancer deaths in the world. The national malignant tumor incidence rate is 250.28 / 100,000 (277.77 / 100,000 for ...

Claims

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Application Information

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IPC IPC(8): A61K35/17A61P35/00A61P35/04A61P37/04A61P37/02C12N5/0783C12N5/10A61K35/15
CPCA61K35/17A61K35/15C07K14/4748C12N5/0638C12N2501/2301C12N2501/2302C12N2501/24C12N2501/515C12N2502/1121C12N2506/11A61K2300/00
Inventor 周彤
Owner SHANGHAI PRECISION DIAGNOSTICS CO LTD
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