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160 results about "Operon gene" patented technology
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In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter.
Promoter and plasmid system for genetic engineering
This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
Owner:EI DU PONT DE NEMOURS & CO
Method for producing l-amino acid by fermentation
InactiveUS20060216796A1Improve abilitiesEnhancing threonine biosynthetic pathwayBacteriaFermentationL-threonineMicrobiology
L-threonine or L-isoleucine is produced by culturing a bacterium which belongs to the genus Escherichia and has an ability to produce L-threonine or L-isoleucine, and wherein expression of a threonine operon is directed by its native promoter, and from which at least a leader sequence and an attenuator are deleted, in a medium and collecting the L-threonine or L-isoleucine from the medium.
Owner:AJINOMOTO CO INC
Method for propagating adenoviral vectors encoding inhibitory gene products
The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.
Owner:GEN VEC INC
Transgenic microbial polyhydroxyalkanoate producers
InactiveUS6913911B2Simple production processInduce expressionSugar derivativesBacteriaBiotechnologyTransposon mutagenesis
Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein mutiple genes are incorporated, these are incorporated as an operon. Sequences are used to stabilize mRNA, to induce expression as a function of culture conditions (such as phosphate concentration), temperature, and stress, and to aid in selection, through the incorporation of selection markers such as markers conferring antibiotic resistance.
Owner:CJ CHEILJEDANG CORP
Bioinformatically detectable group of novel regulatory oligonucleotides and uses thereof
ActiveUS20070042380A1Sugar derivativesMicrobiological testing/measurementNucleotideSpecific function
The present invention relates to a first group of novel oligonucleotides, here identified as “Genomic Address Messenger” or “GAM” oligonucleotide, and a second group of novel operon-like polynucleotides, here identified as “Genomic Record” or “GR” polynucleotide. GAM oligonucleotides selectively inhibit translation of known “target” genes, many of which are known to be involved in various diseases. Nucleic acid molecules are provided respectively encoding 122,764 GAM oligonucleotides and their respective precursors, and 18602 GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.
Owner:ROSETTA GENOMICS
Transgenic microbial polyhydroxyalkanoate producers
Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein mutiple genes are incorporated, these are incorporated as an operon. Sequences are used to stabilize mRNA, to induce expression as a function of culture conditions (such as phosphate concentration), temperature, and stress, and to aid in selection, through the incorporation of selection markers such as markers conferring antibiotic resistance.
Owner:METABOLIX
Bioinformatically detectable group of novel regulatory bacterial and bacterial associated oligonucleotides and uses thereof
InactiveUS20070031843A1Preventing and treating bacterial diseasesSugar derivativesMicrobiological testing/measurementNucleotideSpecific function
The present invention relates to a first group of novel bacterial and human associated oligonucleotides, here identified as “Genomic Address Messenger” or “GAM” oligonucleotide, and a second group of novel operon-like bacterial and human polynucleotides, here identified as “Genomic Record” or “GR” polynucleotide. GAM oligonucleotides selectively inhibit translation of known “target” genes, many of which are known to be involved in various bacterial infections. Nucleic acid molecules are provided respectively encoding 21,916 bacterial and 6,100 human GAM precursor oligonucleotides, and 6,056 bacterial and 430 human GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.
Owner:ROSETTA GENOMICS
Nontypeable Haemophilus influenzae virulence factors
InactiveUS7306805B2Translation is prevented and reducedAvoid stickingAntibacterial agentsSenses disorderVirulent characteristicsOperon
The invention relates to a mutation within the sap operon of an avirulent clone of a nontypeable strain of Haemophilus influenzae (NTHi). The invention also relates to the NTHi sap operon genes and the polypeptides encoded by these polynucleotide sequences. The invention also relates to a novel 110 kDa NTHi outer membrane protein and the polynucleotide that encodes this outer membrane protein. Methods of screening for NTHi infection, and treating and preventing NTHi related disorders are also contemplated.
Owner:NATIONWIDE CHILDRENS HOSPITAL
Vectors, cells and processes for pyrimidine deoxyribonucleosides production
Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin. In preferred embodiments, constructs further comprise DNA sequences encoding for thymidylate synthase and / or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, host cells comprising constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.
Owner:SMITHKLINE BECKMAN CORP
Bioinformatically detectable group of novel regulatory viral and viral associated oligonucleotides and uses thereof
ActiveUS7777022B2Preventing and treating viral diseasesPreventing and treatingSugar derivativesMicrobiological testing/measurementSpecific functionNucleotide
The present invention relates to a first group of novel viral and human associated oligonucleotides, here identified as “Genomic Address Messenger” or “GAM” oligonucleotide, and a second group of novel operon-like viral and human polynucleotides, here identified as “Genomic Record” or “GR” polynucleotide. GAM oligonucleotides selectively inhibit translation of known “target” genes, many of which are known to be involved in various viral diseases. Nucleic acid molecules are provided respectively encoding 1,655 viral and 105,537 human GAM precursor oligonucleotides, and 190 viral and 14,813 human GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.
Owner:ROSETTA GENOMICS
Transformant for reducing weight and fat and preparation method thereof
InactiveCN101608172AGood curative effectOvercome costsBacteriaMetabolism disorderBifidobacteriumProkaryotic expression
The invention relates to a transformant for reducing weight and fat, which is obtained by converting recombinant expression plasmids of a human gastric acid regulin gene into probiotic bacteria of human beings or animals, wherein the recombinant expression plasmids of the human gastric acid regulin gene are obtained by cloning a genetic fragment for coding human gastric acid regulin to an expression area of prokaryotic expression plasmids taking an ara operon as a promoter; and the probiotic bacteria of human beings or animals are bifidobacteria or lactic acid bacteria. The transformant can induce, express and excrete gastric acid regulin inside and outside intestinal tracts through revulsant L, namely, arabinose or low-polyxylose and plays a role of reducing weight and fat, therefore, the transformant can be prepared into an oral live bacterial preparation for reducing weight and fat.
Owner:SOUTHERN MEDICAL UNIVERSITY
Mutant lactobacillus bulgaricus strains free from beta-galactosidase activity
InactiveUS20050196388A1Increase capacityReduced post-acidificationBiocideMilk preparationBiotechnologyBeta-galactosidase activity
The invention concerns mutant L. bulgaricus strains bearing a nonsense mutation, in at least one of the sequences coding for the lactose operon, and free from β-galactosidase activity, and lactic starters comprising these strains. The strains and starters can be used to obtain fermented milk products from glucose-added milk.
Owner:DANONE
Biosensor for small molecule analytes
InactiveUS20060292581A1High sensitivityStrong specificityBioreactor/fermenter combinationsBiological substance pretreatmentsEscherichia coliPhenylarsine oxide
A biosensor device for detecting small molecules analytes is provided. The device employs a first class of molecules, e.g., protein that binds to both the analyte and a second class of molecules, e.g., nucleic acid. The binding of the protein to the analyte and nucleic acid can be mutually exclusive, and the presence of analyte in a sample results in a detectable displacement of protein from nucleic acid. Alternatively, binding of the protein to the nucleic acid can depend on the presence of analyte in the sample. In a specific embodiment, either the protein or nucleic acid is immobilized on a solid phase support. An arsenic detection system is exemplified. An ArsR binding sequence from the E. coli ars operon is immobilized on a gold-plated surface. ArsR protein binds to the DNA in the absence of arsenic, and is released in the presence of sodium arsenate or phenylarsine oxide. Protein release results in a change in surface plasmon resonance, and the magnitude or kinetics of the change indicate the concentration of arsenic.
Owner:LAING LANCE
Highly regulable promoter for heterologous gene expression
InactiveUS6030807APromote reproductionModulate transcriptional activityBacteriaSugar derivativesL-ArabinoseOperon gene
The invention relates to an operon encoding enzymes involved in the utilization of L-arabinose, to the promoter derived therefrom, and to expression systems utilizing the promoter. The promoter is particularly useful for expression of DNA sequences in prokaryotes because of their inducibility and repressibility of the promoter. The invention also relates to the enzymes of the operon, and antibodies thereto.
Owner:THE ROCKEFELLER UNIV
Production and secretion of glucose in photosynthetic prokaryotes (cyanobacteria)
InactiveUS7803601B2Reduce crystallinityEasy to degradeBacteriaUnicellular algaeBacteroidesBiotechnology
The present invention includes compositions and methods for making and using an isolated cyanobacterium that includes a portion of an exogenous bacterial cellulose operon sufficient to express bacterial cellulose, whereby the cyanobacterium produces extracellular glucose. The compositions and methods of the present invention may be used as a new global crop for the manufacture of cellulose, CO2 fixation, for the production of alternative sources of conventional cellulose as well as a biofuel and precursors thereof.
Owner:BOARD OF RGT THE UNIV OF TEXAS SYST
Method for producing L-amino acid using bacterium of Enterobacteriaceae family, having nir operon inactivated
A method is provided for producing L-amino acid, such as L-arginine using a bacterium of Enterobacteriaceae family, particularly a bacterium belonging the genus Escherichia, with an inactivated nir operon.
Owner:AJINOMOTO CO INC
Solid state drive memory device comprising secure erase function
InactiveUS9111621B2Memory architecture accessing/allocationRead-only memoriesProcessor elementSolid-state drive
A memory device such as a solid state memory device have a dual-hardware, secure erase feature. A memory controller operating in a memory controller domain provides general memory management and interface operons. Upon receipt of a trigger signal which may be received from a secure supervisor circuit, a separate processor element that is configured to directly access the raw memory cells in the device bypasses the memory controller domain and executes a separately provided secure erase operating system whereby the raw cell data may be erased and rewritten with a predetermined data pattern and whereby the erase operation at the raw cell level may be verified and reported to the user by the processor.
Owner:IRVINE SENSORS
Bistable genetic toggle switch
InactiveUS6841376B2Enhanced inhibitory effectEfficient switchingFungiBacteriaToggle switchRegulator gene
Provided are methods and compositions for regulating gene expression in a cell. The invention provides recombinant genetic toggle switches which contain a first constitutive promoter-regulatory gene operon and a second constitutive promoter-regulatory gene operon. Expression of the regulatory gene from the first operon inhibits expression from the promoter in the second operon, and expression of the regulatory gene from the second operon inhibits expression from the promoter in the first operon. By use of the toggle switch and various switching agents it is possible to reversibly switch the expression of a gene of interest between a stable “on” state and stable “off” state or vice versa via transient exposure to a switching agent.
Owner:NAVY SEC 0F THE UNITED STATES OF AMERICA
Stable Genomic Integration of Multiple Polynucleotide Copies
Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for Promoter carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
Owner:NOVOZYMES AS
Promoter and plasmid system for genetic engineering
This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
Owner:EI DU PONT DE NEMOURS & CO +1
High-yield L-histidine genetically engineered bacterium strain, constructing method and application thereof
InactiveCN110184230AIncreased growth burdenClear genetic backgroundBacteriaMicroorganism based processesEscherichia coliArginine
The present invention provides a high-yield L-histidine genetically engineered bacterium strain and a constructing method thereof. The bacterium integrates a nucleotide sequence of a corynebacterium glutamicum ATP phosphoribosyl transferase HisG mutant encoding gene hisG* represented by SEQ ID NO:1 based on genome of escherichia coli to enhance activity of histidine-synthesizing key enzyme HisG, also increases copy number of histidine operon genes in the genome to enhance a terminal synthesis pathway of histidine, and also integrates an encoding gene lysE of arginine / lysine transportprotein derived from the corynebacterium glutamicum to promote extracellular secretion of the intracellular histidine. The genetically engineered bacterium is used for producing the L-histidine by a fermentation method, can stably produce 40-55 g / L of the histidine by fermentation in a 5L fermentation tank for 40-50 h, has a production intensity reaching 1.0-1.5 g / (Lxh), and has a conversion rate of 0.18-0.22 g histidine / g glucose.
Owner:TIANJIN UNIV OF SCI & TECH +1
Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
InactiveUS20040210966A1High expressionHigh copy numberClimate change adaptationDepsipeptidesPosition effectChloroplast
Owner:AUBURN UNIV +1
Isolation and characterization of the csa operon (ETEC-CS4 pili) and methods of using same
Compositions comprising products of the csa operon, an isolated nucleic acid encoding the csa operon or functional fragments thereof, purified polypeptide products of the csa operon or functional fragments thereof, methods of eliciting an immune response to these products, and methods of producing products of the csa operon are disclosed herein.
Owner:UNIV OF MARYLAND
Method for producing isoprene by utilizing blue algae
ActiveCN103215315AIncrease productionEfficient expressionMicroorganism based processesIsomerasesIsomerasePlant Sources
The invention discloses a method for producing isoprene by utilizing blue algae, and the method comprises the following steps of: (1) expressing an isoprene synthetase coding gene derived from blue gum and an isoprene synthetase coding gene with an optimized codon in the blue algae; (2) applying the promotor of a cpc operon to express a mediate terpenoid synthetase coding gene contained in the blue algae; (3) enhancing the expression of an prenyl pyrophosphate isomerase coding gene; (4) expressing a fusion protein which contains a prenyl pyrophosphate isomerase and an isoprene synthetase; and (5) applying an SMUO tag to express a heterologous protein in the blue algae. The method disclosed by the invention can be used for carrying out genetic modification on the blue algae and expressing the isoprene synthetase (IspS) of a plant source in the blue algae, outstandingly increases the output of isoprene produced through the gene engineering blue algae through multiple methods, greatly widens the application range of the gene engineering blue algae by applying research results to express other heterologous proteins in the gene engineering blue algae and has wide industrial application prospect.
Owner:SHANGHAI RES & DEV CENT OF INDAL BIOTECH
Salmonella enterica presenting c. jejuni n-glycan or derivatives thereof
The present invention relates to Salmonella enterica comprising at least one pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and / or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.
Owner:ETH ZZURICH
Macrolide efflux genetic assembly
Macrolide resistance associated with macrolide efflux (mef) in Streptococcus pneumoniae has been defined with respect to the genetic structure and dissemination of a novel mefE-containing chromosomal insertion element. The mefE gene is found on the 5′-end of a 5.5 kb or 5.4 kb insertion designated mega (macrolide efflux genetic assembly) found in at least four distinct sites of the pneumococcal genome. The element is transformable and confers macrolide resistance to susceptible S. pneumoniae. The first two open reading frames (ORFs) of the element form an operon composed of mefE and a predicted ATP-binding cassette homologous to msrA. Convergent to this efflux operon are three ORFs with homology to stress response genes of Tn5252. Mega is related to mefA-containing element Tn1207.1. Macrolide resistance due to mega has been rapidly increased by clonal expansion of bacteria containing it and horizontally by transformation of previously sensitive bacteria.
Owner:EMORY UNIVERSITY
Bioluminescent Plants Comprising Bacterial Lux Operon and Methods of Making Same
InactiveUS20100192262A1FermentationVector-based foreign material introductionNucleotide sequencingOrganism
In one aspect, the invention relates to a transgenic bioluminescent plant including an expressible heterologous nucleotide sequence comprising a bacterial LUX operon, which includes LUX A, LUX B, LUX C, LUX D, LUX E, and LUX G genes, wherein the heterologous nucleotide sequence is expressed to render the plant bioluminescent.
Owner:BIOGLOW
Engineering bacteria based on ilv attenuator and application of engineering bacteria to isoleucine production
ActiveCN107058323AImprove expression levelDe-attenuation controlBacteriaMicroorganism based processesChemistryDerepression
The invention discloses engineering bacteria based on an ilv attenuator and an application of the engineering bacteria to isoleucine production and provides an ilv attenuator mutant. The ilv attenuator mutant is a DNA molecule obtained by removing 1st-n4th-position nucleotides from the ilv attenuator, wherein n4 is larger than or equal to 128 and smaller than or equal to 147. The invention further protects an ilvLXGMEDA operon gene with feedback derepression, wherein the ilvLXGMEDA operon gene is obtained by removing 1st-n4th positions counting from the first position of the ilv attenuator from the ilvLXGMEDA operon gene. The invention further protects a method for feedback derepression of an ilvLXGMEDA operon in microorganisms. The method comprises the following step: deleting the 1st-n4th positions counting from the first position of the ilv attenuator. With adoption of the scheme, output of isoleucine and isoleucine derivatives can be increased remarkably, and great important application and promotion values are provided for the field of production of isoleucine and isoleucine derivatives.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Genetic engineering bacterium for L-theanine production and fermentation method thereof
ActiveCN109370966AClear genetic backgroundObvious cost advantageBacteriaTransferasesEscherichia coliT7 phage
The invention provides a genetic engineering bacterium for L-theanine production and a fermentation method thereof. A construction method of the genetic engineering bacterium comprises the steps of carrying out single copying on an ribonucleic acid (RNA) polymerase gene T7RNAP derived from T7 phage on an Escherichia coli W3110 genome, wherein the gene is controlled by a xylose promoter; carrying out dual copying on a gamma-glutamyl methylamine synthetase gene gmas derived from methylovorusmays, wherein the gene is controlled by a T7 promoter; knocking out a xylose operon repressor protein genexylR; and knocking out a succinyl CoA synthetase gene sucCD. The genetic engineering bacterium has the beneficial effects that the yield of L-theanine fermented by using the genetic engineering bacterium can be up to 40g / L and the sugar acid conversion rate can be up to 25%; and the L-theanine production method provided by the invention has the advantages of being low in raw material price, shortin cycle, simple and convenient to operate, green and friendly to environment and high in yield and has good industrial application value.
Owner:TIANJIN UNIV OF SCI & TECH +1
Secretion expression of antibiotic peptide cad in bacillus subtilis and expression system of recombination bacillus subtilis
ActiveUS20120009625A1Easy culture conditionIncrease the propagation speedBacteriaPeptidesAntimicrobial peptidesFeed additive
The present invention relates to a method for expressing antimicrobial peptide CAD by means of a recombinant Bacillus subtilis expression system. The SUMO protease expression operon is first artificially synthesized. The protein expression operon genes of Saccharomyces cerevisiae small ubiquitin-related protein is then fused with the antibacterial peptide AD. The fusion protein is further cloned into the pNF11 plasmid to be introduced into Bacillus subtilis, thereby ensuring the induced expression of recombined Bacillus subtilis in shake flasks. The method has the advantages of a simple expression system, large-scale production, low production cost, strong biological activity and no toxic or harmful substance production. Moreover, the method provides a medicine with low price and strong antibacterial capacity for clinic disease prevention and treatment. This invention can also be used as a feedstuff additive.
Owner:LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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