44 results about "DNA glycosylase" patented technology

The invention belongs to the technical field of medicine and chemical industry, and discloses a class of natural catalpol derivatives and a class of new catalpol derivatives derived from catalbin and picroside II modification. The invention also provides the preparation method of the above derivatives and Application in the preparation of anti-inflammatory drugs. The structure of the natural catalpol derivatives involved in the present invention is as shown in formula (I) or formula (II), and the structure of the new catalpol derivatives derived from catalbin and pubetroside II modification is as shown in formula (III) or Shown in formula (IV). Experiments have proved that the compounds of formula (I), formula (II), formula (III) and formula (IV) described in the present invention or their hydrates, pharmaceutically acceptable salts, tautomers, stereoisomers, The precursor compound can significantly inhibit 8-hydroxyguanine DNA glycosylase 1 (8-hydroxytridine DNA glycosylase 1, OGG1), and has a broad application space in the preparation of anti-inflammatory drugs and anti-tumor drugs.

The invention discloses loop-mediated isothermal amplification primers and kit for detecting acanthamoeba. The sequences of the primers are represented by SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4 respectively. The loop-mediated isothermal amplification primers designed according to the sequence of the conserved region of the gene of the acanthamoeba can flexibly, quickly and safely detect acanthamoeba; meanwhile, in the kit disclosed by the invention, false positive caused by nucleic acid pollution in a multiple detection process can be radically eliminated by using uracil-DNA glycosylase (UNG), so the problem of susceptibility to pollution and interference, which limits the wide application of a loop-mediated isothermal amplification technique, is solved. When the kit disclosed by the invention is used, the acanthamoeba causing amebic keratitis can be detected within 3 hours, and instead of expensive instrument and equipment, only a water bath pot or metal bath and a centrifuge are needed in a detection process; the detection result is very easy to determine; and compared with the conventional detection technique, the kit is low in cost, the kit is very safe for operators and the environment for the whole process is free from toxic reagents, and the detection sensitivity of the kit is very high.

The invention discloses a method for ultra-sensitively simultaneously detecting multiple DNA glycosylases by using intrinsic fluorescent nucleotide. The method is a fast and sensitive fluorescence method for simultaneously detecting multiple DNA glycosylases by adopting 2-aminopurine and pyrrole-deoxycytidine as fluorophores and a DNA molecule as an intrinsic quenching agent and combining excision enzyme-assisted circulating signal amplification; and extra fluorophore and quenching group are not needed for marking, so that the target of simple, intuitive and high-sensitivity detection of an actual sample is achieved, and above all, simultaneous detection of multiple DNA glycosylases is achieved.

The invention discloses a prawn IHHNV (infectious hypodermal and hematopoietic necrosis virus) nucleic acid isothermal amplification detection kit which comprises a grinding fluid tube containing a grinding fluid, a nucleic acid extracting solution tube A containing a sodium acetate solution, a nucleic acid extracting solution tube B containing absolute ethyl alcohol, a nucleic acid extracting solution tube C containing an alcohol solution with a mass concentration of 70 percent, a TE (tellurium) buffer solution tube containing a TE buffer solution, a UNG (uracil-DNA glycosidase) enzyme tube containing uracil DNA (deoxyribonucleic acid) glycosylase, an LAMP (Loop-mediated isothermal amplification) reaction liquid tube containing an LAMP reaction liquid, a Bst DNA polymerase tube containing Bst DNA polymerase, a color-developing agent tube containing nucleic acid dye SYBR Green I, a positive control nucleic acid tube containing prawn IHHNV positive DNA and a negative control tube containing sterilizing double distilled water. The invention also provides a method for detecting the prawn IHHNV by utilizing the detection kit. The method has the characteristics of low cost, high detection sensitivity and easy site operation.

The invention provides a sequencing primer pair for qualitatively detecting human BRAF V600E gene mutation and a kit thereof, belonging to the field of detection for in-vitro nucleic acid. The kit comprises a uracil DNA (deoxyribonucleic acid) glycosylase, a Taq polymerase, PCR (polymerase chain reaction) solution, a PCR amplification primer, a pyrosequencing primer and a positive reference substance. The kit provided by the invention is high in sensitivity, good in specificity, capable of sequencing a PCR product on a pyrosequencer after performing a simple treatment, simple and convenient in operation, short in reaction time, higher in sensitivity compared with golden standard-capillary electrophoresis sequencing, and more suitable for mutation analysis.