33 results about "Folate Metabolism" patented technology

The invention discloses application, a detection method and a kit of SNP of an METHFR gene guiding folic acid and related nutrient element intake. Because of providing different positive locuses of SNP gene types of folic acid metabolic capacity and related nutrient element intake, the reason generating impediment thereof is found, and the guidance for guiding individuals, especially pre and post natal women, to take folic acid and intake related nutrients is provided.

The invention discloses a method and a kit for detecting genetic information of folate metabolism related genes, and relates to three sites, namely an MTHFR gene rs1801133 site, an rs1801131 site andan MTRR gene rs1801394 site. The method and kit provided by the invention, on the basis of the comprehensive use of such technologies as direct PCR, nested PCR, multiple PCR, created restriction sitemethod PCR, PCR-RFLP and the like, can implement PCR amplifications by two turns in a mode of directly using cells without extracting DNA, and the second turn of PCR product of each site can use cheaprestriction endonuclease to conduct enzyme digestion typing; in accordance with actual demands, the three sites can be simultaneously detected within a same tube, or two sites can be simultaneously detected within a same tube, or any one of the sites can be independently detected. All PCR products take enzyme digestion internal control sequences as quality control of enzyme digestion, so that theaccuracy of result judging. The method is simple and convenient in operation of a whole process and is low cost; and accuracy is quite guaranteed.

The present disclosure describes the use of genetic variance information for folate transport or metabolism genes or pyrimidine transport or metabolism genes in the selection of effective methods of treatment of a disease or condition. The variance information is indicative of the expected response of a patient to a method of treatment. Methods of determining relevant variance information and additional methods of using such variance information are also described.

The invention relates to a primer pair and a kit for detecting folate metabolism-related gene polymorphism in hypertensive patients, and belongs to the technical field of in vitro nucleic acid detection. The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5' end of the reverse amplification primer is labeled with biotin. Thekit comprises a PCR (Polymerase Chain Reaction) solution containing a forward amplification primer and a reverse amplification primer, a sequencing primer, uracil DNA (Deoxyribonucleic Acid) glycosylase, Taq polymerase and a positive control product. The kit provided by the invention has the advantages of accurate detection result, high specificity, short detection period, and simple operation, and the clinical inspection requirements can be effectively met; in addition, the kit also has the advantages of real-time monitoring of reaction progress, and short reaction time, the PCR product canbe used for sequencing on a pyrosequencing instrument after being simply treated, the sensitivity is higher than that of a gold standard method, i.e., a capillary electrophoresis sequencing method, and the kit is more applicable to mutation analysis.

The invention discloses a liquid chromatography tandem mass spectrometry detection kit and a detection method for testing folic acid metabolism derivatives of a human body, and belongs to the technical field of analysis and detection. The liquid chromatography tandem mass spectrometry detection kit for testing the folic acid metabolism derivatives of the human body can be used for detecting folic acid, tetrahydrofolic acid, 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, 5,10-methylenetetrahydrofolate, S-adenosylmethionine, S-adenosylhomocysteine and homocysteine serum (plasma). The method has the characteristics of simplicity, rapidness, strong specificity, high sensitivity, comprehensive detection indexes and the like; the method can provide a reference basis for knowing the folic acid metabolism condition in clinical application.

The invention provides a kit for detecting the genotyping of folic acid metabolizing genes, and belongs to the technical field of molecular biology. Specifically, RNase H2 and Taq DNA polymerase biphasic enzyme systems were used to detect the folate metabolism-related gene loci. The kit provided by the invention has the advantages of high sensitivity, fast detection, good stability and closed detection, and reduces the possibility of later pollution.

The invention provides a gene detection method of amount of an individual folic acid replenisher. Through design of a primer for amplifying rs1801133, rs1801131, rs1805087 and rs1801394 sites, 4 SNP sites tightly relevant with metabolism of folic acid in individuals are amplified, and the gene type of individual amplification fragments is further analyzed. According to the difference of idiotype, the quantity demanded of the folic acid is recommended. The method is simple to operate and high in practicability, and is suitable for promotion and use.

The invention relates to compound sulfamonomethoxine injection and a preparation method of the compound sulfamonomethoxine injection. The injection comprises main drug, antioxidant, solvent for injection and water for injection. The preparation method is that the sulfamonomethoxine and trimethoprim (TMP) are used together, which can doubly block folate metabolism of bacteria; under the bidirectional cooperative effect between cyclooxygenase and ethyl inhibitor, the sulfamonomethoxine and doxycycline cooperate, so the drug absorption is fast and the toxicity is reduced with better efficacy and more safety. With scientific compatibility, lower cost and simple and easy preparation method, the invention is suitable for industrialized mass production.

The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least(a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest;(b) a polynucleotide encoding a first selectable marker (sm I);(c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I),wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

The invention provides a method for detecting polymorphism of folate metabolism-related genes. The method comprises the steps that specific amplification primers and single base extension primers of the C677T site and A1298C site of an MTHFR gene and the A66G site of an MTRR gene are synthesized in advance; to-be-detected sample genomic DNA is extracted from a to-be-detected sample to serve as a DNA template; a polymerase chain reaction PCR amplification system containing the specific amplification primers and the DNA template is prepared; a PCR apparatus is used for amplifying the PCR amplification system; digestion treatment is performed in sequence; a single base extension reaction system containing the single base extension primers is added into a reaction system for extension reactions in sequence, desalted purification treatment is performed in sequence, and a purified product is obtained; a detection instrument containing a mass spectrometer is used for detecting the purified product to determine the gene polymorphism of the C677T site and A1298C site of the MTHFR gene and the A66G site of the MTRR gene of the to-be-detected sample. The method for detecting the polymorphismof the folate metabolism-related genes can improve the detection efficiency of folate metabolism capacity in the body of a patient.

The invention discloses crRNA, a kit and a method for detecting folate metabolism related molecular marker gene mutation. The crRNA comprises crRNA of a mutation site of MTHFR-667 with a sequence as shown in any one of SEQ ID NO.11-12; crRNA of a mutation site of MTHFR-1298 with a sequence as shown in any one of SEQ ID NO.13-14; and crRNA of a mutation site of MTRR-66 gene with a sequence as shownin any one of SEQ ID NO. 15-16. By screening crRNA and combining with a CRISPR-Cpf1 system, whether folate metabolism related molecular marker gene mutation exists in a to-be-detected sample or not can be detected in a short time, the kit and the detection method are easy to operate, high in detection speed and low in cost, repeated detection can be achieved, and meanwhile detection sensitivity and detection specificity are remarkably improved.

The invention discloses a method for detecting folate metabolism-related gene. The method comprises the following steps: subject nucleic acid is obtained, the nucleic acid comprises DNA fragment; at least two SNP bits genotype are detected, MTRR gene c.66A>G; MTHFR gene c.667C>T; and MTHFR gene c.1298A>C; a first primer amplification comprise the DNA fragment of the SNP bit, the amplification product is obtained, a second primer is combined with the amplification product, a base is extended, an extension product is obtained, according to the molecular weight difference of the extension product and the second primer, the base type of the SNP bit is determined. The invention also discloses a kit for detecting folate metabolism capability gene. By using the method and / or the kit, the detecting folate metabolism-related gene can be typed with high flux, low cost, high accuracy and short time consumption, and the typing result can help the subject for supplying folic acid.

The invention belongs to the field of biomedicine, solves the problem that one-way single base extension accurately determines a mutation site difficultly, and discloses a method for detecting a mutation site of a folate metabolism related gene. A target fragment is obtained through a single-tube multiplex PCR amplification reaction of a designed target site specific primer, then the target fragment is bidirectionally extended by using a single base extension technology to obtain a to-be-detected gene site, and finally a target DNA sequence is accurately determined through the nucleic acid mass spectrum analysis of the to-be-detected gene site, wherein the single base extension technology involves that a single base extension primer is adopted for bidirectionally extending the target fragment. The invention aims to perform mutation detection on the folate metabolism related gene by the bidirectional single base extension technology, thereby providing safety guidance for individual clinical medication.

The invention provides a method for increasing the content of 5-methyltetrahydrofolate through gene knockout. Maize by CRISPR / Cas9 technology ZmGFT1 The gene knockout was implemented, and it was found that the content of 5-methyltetrahydrofolate in maize was significantly increased after the gene knockout. Corn in the present invention ZmGFT1 The design of the CRISPR / Cas9 target site and the discovery that the function of the gene can significantly affect the folic acid content not only provide a reference for the CRISPR research of this gene in maize and other crops, but also provide a theory for the folic acid metabolism of maize and other crops The foundation, by increasing the folic acid content of transgenic plants, alleviates the hidden hunger problem of human beings in folic acid nutrition.

The invention relates to a pharmaceutical composition for preventing and treating the black gill disease of aquatic animals. The pharmaceutical composition is prepared from the following components in parts by weight: 50 parts of cortex dictamni, 75 parts of common cnidium fruit, 50 parts of golden larch bark, 40 parts of artemisia leaves, 45 parts of sophora flavescens, 45 parts of fineleaf schizonepeta herb, 25 parts of ephedra, 30 parts of common threewingnut root and 25 parts of gypsum rubrum. In the invention, the synergy of all the components is exerted through selecting and reasonably proportioning the pharmaceutical compositions, bacterial growth and reproduction are inhibited through interfering the protein synthesis and the folate metabolism of sensitive pathogenic microorganisms, and the pharmaceutical composition can be used for preventing and treating symptoms of aquatic animals caused by various bacteria and fungi, such as black gills, yellow gills, gill rotting, brown spots, tail rotting, body rotting, and the like. The invention is applied to remarkably improving the water quality, obviously adding the feed quantity and obviously reducing unintelligible death; and the special natural biological active substances in the pharmaceutical composition can control the metabolism of organisms and help the aquatic animals recover. Meanwhile, the pharmaceutical composition provided by the invention is a pure Chinese medicine preparation without any toxic and side effects and ca not form pharmaceutical residue in the aquatic animals.