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Liquid chromatography tandem mass spectrometry detection kit and detection method for testing folic acid metabolism derivatives of human body

A detection kit and folic acid metabolism technology, applied in the field of analysis and detection, can solve problems such as the inability to detect multiple derivatives at the same time

Pending Publication Date: 2021-06-22
广东南芯医疗科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemiluminescence cannot simultaneously detect multiple derivatives in folic acid metabolism
On the mass spectrometry platform, in previous reports, the combination of folic acid and homocysteine, cysteine, etc., or the combination of folic acid and 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, etc., can only derivate folic acid metabolism. One of the impact path indicators of the object is tested

Method used

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  • Liquid chromatography tandem mass spectrometry detection kit and detection method for testing folic acid metabolism derivatives of human body
  • Liquid chromatography tandem mass spectrometry detection kit and detection method for testing folic acid metabolism derivatives of human body
  • Liquid chromatography tandem mass spectrometry detection kit and detection method for testing folic acid metabolism derivatives of human body

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Experimental program
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Embodiment 1

[0050] A liquid phase tandem mass spectrometry detection kit for testing human folic acid metabolic derivatives, comprising the following components:

[0051]

[0052] Folic acid, folic acid internal standard, tetrahydrofolate, 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, 5,10-methylenetetrahydrofolate, homocysteine, homocysteine ​​internal standard , S-adenosylmethionine, S-adenosylhomocysteine ​​chromatograms see Figure 1-10 ;Standard folic acid, tetrahydrofolate, 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, 5,10-methylenetetrahydrofolate, homocysteine, S-adenosylmethionine , S-adenosylhomocysteine ​​total ion chromatogram see Figure 11 . Wherein, the abscissa of the chromatogram is time, and the unit is min; the ordinate is the response value.

[0053] The preparation method is:

[0054] ①PBS buffer:

[0055] Weigh 0.24g of potassium dihydrogen phosphate, 1.44g of disodium hydrogen phosphate, 8g of sodium chloride, and 0.2g of potassium chloride, add a...

Embodiment 2

[0075] Example 2 A liquid phase tandem mass spectrometry detection method for testing human folic acid metabolic derivatives

[0076] (1) Sample collection

[0077] Collect blood (serum or plasma) on an empty stomach according to the current venous blood collection method, and all samples should be treated as blood-borne infectious disease specimens.

[0078] (2) Sample processing

[0079] Take 100-200 μL of sample (serum, calibrator or quality control product) into the Ep tube, add 5 μL of internal standard solution, then add 500 μL of extraction solution 1, vortex for 1 min, and mix well. After centrifugation, transfer 400 μL of the supernatant to a 96-well injection plate. Blow dry with nitrogen. Add 100 μL of reconstitution solution 2 to redissolve, cover with aluminum foil, and test for later use. Put the 96-well sampling plate into the autosampler, ready to go on the machine.

[0080] (3) Machine conditions

[0081] ① Chromatographic conditions

[0082] Chromatogr...

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Abstract

The invention discloses a liquid chromatography tandem mass spectrometry detection kit and a detection method for testing folic acid metabolism derivatives of a human body, and belongs to the technical field of analysis and detection. The liquid chromatography tandem mass spectrometry detection kit for testing the folic acid metabolism derivatives of the human body can be used for detecting folic acid, tetrahydrofolic acid, 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, 5,10-methylenetetrahydrofolate, S-adenosylmethionine, S-adenosylhomocysteine and homocysteine serum (plasma). The method has the characteristics of simplicity, rapidness, strong specificity, high sensitivity, comprehensive detection indexes and the like; the method can provide a reference basis for knowing the folic acid metabolism condition in clinical application.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, and more specifically relates to a liquid phase tandem mass spectrometry detection kit and a detection method for testing human folic acid metabolic derivatives. Background technique [0002] Folate is a general term for a class of substances, including folic acid and its derivatives 5-formyltetrahydrofolate (5-FoTHF, folinic acid), 5-methyltetrahydrofolate (5-MeTHF ), 5,10-methylenetetrahydrofolate and tetrahydrofolate (THF), etc. Folic acid mainly affects the two processes of DNA synthesis and DNA methylation. On the one hand, it is the conversion of uracil deoxynucleotide (dUMP) to thymine deoxynucleotide (dTMP). dUMP is accumulated due to folic acid deficiency and is misincorporated DNA. A large amount of incorporated dUMP can easily induce gene mutations, DNA single- and double-strand breaks, chromosome breaks and other genomic stability decline events and cell malignant tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 张召刁嘉茵郑康帝陈涛
Owner 广东南芯医疗科技有限公司
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