Kit for genotyping folate metabolism gene

Abstract

The invention provides a kit for genotyping folate metabolism gene. The kit includes a PCR reaction liquid and a DNA hybrid membrane strip, the DNA hybrid film strip comprises a matrix vector and probes, four probes for MTHFR genes, two probes for MTRR genes and two probes designed for human genome are sequentially fixed on the matrix vector, each of the probes is an oligonucleotide sequence hybridized with the SNP locus of each of the corresponding gene, and the nucleotide sequences of the probes are represented by SEQ ID NO:1-8. The kit provided by the invention has the advantages of high sensitivity, rapid detection, good stability, realization of high or low flux detection, high flexibility, strong maneuverability for small clinical samples in some hospitals, less equipment investment and low cost.

Description

technical field [0001] The invention relates to a gene detection technology related to folic acid metabolism ability, which belongs to the field of molecular biology gene chip technology, specifically belongs to the SNP polymorphism detection method in the field of in vitro diagnostic reagents, and more specifically uses multiple polymerase chain reaction technology and Reverse dot hybridization technique. Background technique [0002] China is one of the countries with a high incidence of birth defects in the world, and the number of children with birth defects every year accounts for about 20% of the world. Common birth defects in my country include neural tube defects, congenital heart disease, cleft lip and palate, and hypospadias. According to statistics from the Chinese Center for Disease Control and Prevention, there are 800,000 to 1.2 million birth defects every year, and an average of one defect is born every 30 seconds. Among them, in addition to 20%-30% of child...

AI Technical Summary

Problems solved by technology

[0011] Genetic variants such as MTHFR and MTRR cause corresponding reductions in enzyme activity that inhibit the conversion of homocysteine ​​to methionine, leading to hypofolate and hyperhomocysteinemia, thereby increasing the risk of birth defects in newborns risk of spontaneous miscarriage

[0012] At present, the method of folic acid-related gene detection on the market is mainly sequencing method. Although sequencing method is the "gold standard" of gene detection, the detection cycle of sequencing method is long. Clinically, for patients, they hope to get it as soon as possible. The better the test results, the sequencing method is not conducive to the clinical detection of genes related to folic acid metabolism

Moreover, the cost of sequencing is relatively high, which is very high for patients and related testing institutions, which further limits its clinical application

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