44 results about "Viral enteritis" patented technology

The invention discloses a traditional Chinese medicine additive for preventing and treating porcine viral enteritis, which comprises the following raw materials: Astragalus membranaceus, Radix Codonopsitis, radix sileris, Poria cocos, Atractylodes macrocephala, aucklandia roots, Magnolia officinalis, evodia, dried ginger, Fructus Psoraleae, raw hawkthron, goose grasses, honeysuckle, isatis roots, swamp mahogany, dyers woad leaves, radix scutellariae, coptis roots, pawpaw, garlicin, borneol and liquorice. After being eaten by pigs, the traditional Chinese medicine additive can have the function of health protection, improve the immunologic function and the disease resistance of the pigs, and prevent and treat porcine viral enteritis, wherein the disease rate is 8 percent, and the mortality rate is 1 percent; and the effective rate for treating porcine viral enteritis is 98 percent, and the cure rate is 97 percent. The raw materials have wide source and low cost, and are safe and nontoxic; no drug resistance is generated; and pork has no drug residue, so that the traditional Chinese medicine additive for preventing and treating porcine viral enteritis is an ideal product for replacing the prior traditional Chinese medicine additive.

The invention relates to a duck viral enteritis virus infective cloned system, which is characterized in that the system comprises multiple cosmids, each cosmid possesses clone of a gene fragment of duck viral enteritis viral vaccine strain, the gene fragment of the duck viral enteritis viral vaccine strain contains a mutual overlapped area, and splices to cover the whole gene group of the duck viral enteritis viral vaccine strain. The invention also relates to a construction method and an application of the duck viral enteritis virus infective cloned system.

The invention provides a recombinant duck enteritis virus (DEV) vaccine strain CCTCC NO:V201125 named rDEVus78Ha-Re6 for expressing an avian influenza virus haemagglutinin (HA) gene, and a constructing method and application thereof. The vaccine strain is prepared by the following steps of: inserting a genetic fragment SV40-HA comprising the avian influenza virus HA gene and an SV40 promoter sequence into a spacer between a US7 gene and a US8 gene of a DEV by adopting a recombinant cloning technology; constructing a cosmid for inserting an SV40-HA expression frame between the US7 gene and theUS8 gene; and saving to obtain the recombinant DEV vaccine strain CCTCC NO:V201125 for expressing the avian influenza virus HA gene. The invention further relates to a method for constructing the recombinant DEV vaccine strain and application of the recombinant DEV vaccine strain to preparation of a vaccine for preventing duck viral enteritis and avian influenza.

The invention relates to a duck enteritis virus infectious recombinant cloning system. The duck enteritis virus infectious recombinant cloning system is characterized by comprising a plurality of cosmids, wherein a duck enteritis virus vaccine strain gene segment is cloned in each cosmid, contains areas which are overlapped with each other, and a duck enteritis virus vaccine strain complete genome is assembled and covered; and an exogenous gene is inserted in a replication nonessential region of at least one cosmid. The invention also relates to a method for constructing the duck enteritis virus infectious recombinant cloning system and application of the duck enteritis virus infectious recombinant cloning system to the construction and recombination of the duck enteritis virus.

The invention provides a method for quickly detecting duck viral enteritis by real-time fluorescence quantitative PCR. The method selects a gene fragment of duck viral enteritis UL31 to design a primer and a probe, and order-checking results on an amplified fragment proves that homologies of the amplified fragment and the gene fragment are both 100 percent without a cross reaction, thereby eliminating problems of virus variation and false negative caused by region factors, and improving detectable positive rate and compatibility of the method. The method constructs a quantitative positive quality control standard (recombinant plasmid pMD18-T-DEV76bp), establishes a standard curve of fluorescence quantitative detection, and can minimally detect 2.1*10 degrees copy / mu liter. The assembled standard kit reacts under a standard reaction condition, and determines according to accurate quality control standard and determination standard. The method has the advantages of strong specificity, high sensibility, safe quantitative positive quality control stand, simple operation and low cost, is applicable to large-scale quarantine inspection, port quarantine inspection, epidemiological investigation and laboratory quick diagnose of field duck viral enteritis.

The invention provides a recombinant duck viral enteritis virus vaccine for expressing secretory duck Tembusu virus M / E protein, and a construction method and application thereof. The collection number is CCTCC NO:V201215, and the name is rDEV-TME-tPAS. By using a recombinant clone technique, a gene segment rDEV-TME-tPAS comprising an SV40 promoter, duck Tembusu virus M and E proteins and a tPA (Tissue plasminogen activator) signal peptide sequence is inserted into a spacer between the US7 and US8 genes of the duck viral enteritis virus to construct a cosmid in which the rDEV-TME-tPAS expression frame is inserted between the US7 and US8 genes, thereby obtaining the recombinant duck viral enteritis virus vaccine CCTCC V201215 for expressing secretory duck Tembusu virus M / E protein. The invention also provides a method for constructing a recombinant duck viral enteritis virus vaccine strain and application of the recombinant duck viral enteritis virus vaccine strain in preparing a vaccine for preventing infectious diseases caused by duck viral enteritis virus and duck Tembusu virus.

The invention discloses an anti-canine parvovinis disease preparation which is characterized in that the preparation is prepared by the weight part from 25 to 35 parts of lalang grass rhizome, 25 to 35 parts of ailanthus altissima swingle, 30 to 40 parts of liquiritia glycyrrhiza, 40 to 60 parts of radix isatidis, 40 to 60 parts of baical skullcap root and 30 to 50 parts of pulsatilla chinensis, and has comparatively significant effect of curing the canine parvovinis viral enteritis.

The invention provides a recombinant duck viral enteritis virus vaccine for expressing secretory duck Tembusu virus E protein, and a construction method and application thereof. The collection number is CCTCC V201214, and the name is rDEV-TE-tPAS. By using a recombinant clone technique, a gene segment SV40-E-tPA comprising an SV40 promoter, a duck Tembusu virus E protein and a tPA (Tissue plasminogen activator) signal peptide sequence is inserted into a spacer between the US7 and US8 genes of the duck viral enteritis virus to construct a cosmid in which the SV40-E-tPA expression frame is inserted between the US7 and US8 genes, thereby obtaining the recombinant duck viral enteritis virus vaccine CCTCC V201214 for expressing secretory duck Tembusu virus E protein. The invention also provides a method for constructing a recombinant duck viral enteritis virus vaccine strain and application of the recombinant duck viral enteritis virus vaccine strain in preparing a vaccine for preventing infectious diseases caused by duck viral enteritis virus and duck Tembusu virus.

The invention relates to a traditional Chinese medicine composite preparation for effectively controlling the fur animal viral diseases. The preparation is composed of the following nine traditional Chinese herbals by weight: 20 to 24 grams of radix astragali, 5 to 12 grams of white peony root, 10 to 12 grams of Chinese angelica, 8 to 12 grams of bighead atractylodes rhizome, 5 to 12 grams of poria cocos, 3 to 6 grams of cassia twig, 1 to 3 grams of Sichuan pepper, 8 to 10 grams of saposhnicovia divaricata, and 6 to 9 grams of licorice. The preparation method of the composite preparation comprises the following steps: selecting roots, stalks, and seeds of pure natural high-quality traditional Chinese herbals, individually grinding the selected traditional Chinese herbals by a traditional Chinese herbal grinder under a clean and dry condition, sieving the grinded traditional Chinese herbals by a 100-mesh sieve, and finally evenly mixing the processed traditional Chinese herbals according to the weight ratio mentioned above. The composite preparation can be used along with feed, and can also be orally taken after being decocted with water. The composite preparation has the advantages of user-friendliness, stable curative effect, no side or toxic effect, and no drug residue. The composite preparation is mainly used to prevent and treat viral diseases (canine distemper, viral enteritis, etc.) of fur animals such as mink, fox, racoon dog, and the like, and has a high practical and promotion value.

A traditional Chinese medicine preparation for treating viral enteritis and diarrhea relates to a Chinese patent medicine product for treating viral enteritis and diarrhea, belongs to the technical field of Chinese veterinarians, and is mainly used for canine parvovirus, diarrhea caused by animal bacterial, viral and parasitic injection, and physiological peptic diarrhea. The traditional Chinese medicine preparation has following components: 50 g rhizoma coptidis, 30 g atractylis ovata, 50 g terminalia chebula, 30 g Chinese violet, 30 g cyrtomium rhizome, 25 g hairyvein agrimony, 30 g magnolia officinalis, 60 g allium sativum, 25 g radix saposhnikoviae and 25 g magnolia officinalis. The traditional Chinese medicine preparation has the effects of fever reducing, detoxication, inflammation diminishing, sterilization, virus inhibiting, desinsectization, diarrhea checking, constringency, hemostasis, spleen strengthening, qi tonifying, diuresis promoting, spleen invigorating and qi replenishing. The traditional Chinese medicine preparation can not only treat diarrhea caused by animal bacterial, viral and parasitic injection and protect the intestinal mucosa, but also has no drug resistance, and the effect is greatly superior to the chemical drug and single traditional Chinese medicine preparations used at present. Active ingredients in medicine are extracted through adopting a technology of applying the organic solvent extraction method, and the dosage use is 30% of original drug.

A method for immunizing the meat geese in their centralized culture procedure in order to increase their survival rate includes such steps as injecting the double yolk liquid of small goose pest and viral enteritis in one-day age, injecting the double vaccine of colibasillus and fowl's cholera vibro in 3- day age, injecting the double vaccine of fowl's influenza virus and paramyxovirus in 5-day age, injecting the double vaccine of fowl's influenze virus and paramyxovirus in 15-day age, and injecting duck pest vaccine in 20-day age.

The invention provides a recombinant duck viral enteritis virus, a preparation method and applications, belonging to the field of animal medicine vaccines. The avian influenza virus haemagglutinin gene is 1437th to 3131th nucleotide with the nucleotide sequence of SEQ ID NO:1. According to the recombinant duck viral enteritis virus, an expression cassette containing the avian influenza virus haemagglutinin gene is inserted in a spacer region between LORF11 and UL55 genes of duck viral enteritis virus genome. As a vaccine active ingredient, the avian influenza virus haemagglutinin gene can achieve better cross protection effect. The recombinant duck viral enteritis virus can stably and efficiently express the avian influenza virus haemagglutinin gene, virus is stable and grows well, the prepared live vaccine has higher protective efficacy on duck plague and avian influenza, higher avian influenza antibody is generated, and the duration period of the antibody is long.

The invention discloses a Chinese medicine composition as well as a preparation and application thereof, relates to the field of traditional Chinese medicines and in particularly relates to a Chinese medicine composition for treating infantile diarrhea and a preparation of the Chinese medicine composition. The invention aims at solving the problems in the prior art of limited medicines for treating the infantile diarrhea and toxic and side effects in different degrees and provides the Chinese medicine composition as well as the preparation and the application thereof. The Chinese medicine composition is prepared from the raw materials in parts by weight: 300 parts of fired yam, 150 parts of poria cocos, 100 parts of kudzu, 100 parts of rhizoma atractylodis, 100 parts of dark plum and 50 parts of mangnolia officinalis. The invention also provides the application of the Chinese medicine composition for preparing the medicines for treating non-bacterial infectious infantile diarrhea. The Chinese medicine composition and the preparation have special effects in treating viral enteritis and noninfective diarrhea in the infantile diarrhea.

The present invention discloses a traditional Chinese medicinal herb preparation, which is prepared with ginseng, Chinese caterpillar fungus, lucid ganoderma, velvet antler, tortoise shell, beef tendon, astragalus root, divaricate saposhnikovia root, kudzuvine root, Indian buead, costus root, semen plantaginis, fried rhizoma atractylodis macrocephalae, medcinal evodia fruit, rhizoma zingiberis, malaytea scurfpea fruit , fossilizid, oyster, hawthorn fruit, goosegrass herb, indigowoad root , eucalyptus robusta smith, common andrographis herb, baical skullcap root, golden thread, borneol and liquoric root. The traditional Chinese medicinal herb preparation can promote infantile digestive absorption, stimulate appetite, protect health, enhance the immunologic function and disease-resisting capability of infants and prevent and treat infantile virus enteritis, and the effective rate can reach 95 percent and 98.6 percent in preventing and curing infantile virus enteritis. The traditional Chinese medicinal herb preparation, which has the advantages of safety, non-toxicity and no generation of drug fastness, is an ideal traditional Chinese medicinal herb preparation for preventing and curing infantile virus enteritis.

The invention discloses drug for the treatment of abdominal pain and diarrhea and belongs to the field of TCM. The drug comprises pharmaceutical effective ingredients, including Chinese granesbill, turtle shell, Eryngium foetidum herb, multibract raspberry root, cutch, birch bark, radish seed, Asiatic pennywort herb, common turczaninowia herb, Russian olive fruit, purple flower holly leaf, coastal heritiera seed, chalk, cucubalus root, Yunnan Pistache leaf, and quince. The drug has the effects of regulating qi to alleviate pain, clearing away heat and eliminating dampness, soothing the liver to dispel melancholy and tonifying the spleen to cure diarrhea, and has the significant effect of the diarrhea, watery stool, abdominal pain and poor appetite caused by damp-heat accumulation and the viral enteritis and non-infectious diarrhea caused by rotavirus. The drug has the advantages that the preparation process is simple, curative effect is good and fast, the drug is convenient to take and available for treating both symptoms and root causes, and the drug is neutral in property and has no toxic and side effects; the drug has good effects for the treatment of inappetence, nausea and vomiting, abdominal distension and diarrhea, and dyspepsia.

The invention discloses a PCR method for detecting duck viral enteritis. The method comprises the following steps: gene fragments of duck viral enteritis UL30 and UL31 are selected; a pair of primers are determined through screening; PCR amplification is carried out by using virus DNA extracted from standard strain and various wild strains as a template to obtain about 446bp strips respectively; sequencing results are compared with those announced in UL30 and UL31 regions, homology reaches 100 percent, variation phenomenon is not found, and 6 duck susceptible viruses and 3 similar viruses do not have response. DNA extract of each tissue can be detected by the established PCR method, and DNA fragments with size about 446bp can be amplified with minimum detection capacity of 2.1fg (or copy). The method is applicable to clinical and port detection, has the advantages of high sensibility, quick specificity, convenient operation and easy standard; the assembled standard kit has the advantages of stable performance, convenient and quick operation and high amplification yield, is safer by using C-KCE strain as positive control, and establishes accurate determination standard.

The invention is specifically used for treating diarrhoea among children caused by viral enteritis or bacterial enteritis. The invention is characterized in that the invention is a powder mixed by an Acetophenone piperidine compound, a Cicang tablet, a silicon-carbon tablet, a DHCT tablet, an amber powder, a single Naerbin and a Marin guanidine tablet or an oxytetracycline tablet. Taking the medicine three times a day, children can stop diarrhoea, defever and then have a good appetite; so that the invention can be described as stopping diarrhoea quickly.

The invention discloses a traditional Chinese medicine additive for preventing and treating porcine viral enteritis, which comprises the following raw materials: Astragalus membranaceus, Radix Codonopsitis, radix sileris, Poria cocos, Atractylodes macrocephala, aucklandia roots, Magnolia officinalis, evodia, dried ginger, Fructus Psoraleae, raw hawkthron, goose grasses, honeysuckle, isatis roots,swamp mahogany, dyers woad leaves, radix scutellariae, coptis roots, pawpaw, garlicin, borneol and liquorice. After being eaten by pigs, the traditional Chinese medicine additive can have the function of health protection, improve the immunologic function and the disease resistance of the pigs, and prevent and treat porcine viral enteritis, wherein the disease rate is 8 percent, and the mortalityrate is 1 percent; and the effective rate for treating porcine viral enteritis is 98 percent, and the cure rate is 97 percent. The raw materials have wide source and low cost, and are safe and nontoxic; no drug resistance is generated; and pork has no drug residue, so that the traditional Chinese medicine additive for preventing and treating porcine viral enteritis is an ideal product for replacing the prior traditional Chinese medicine additive.

The invention relates to the field of veterinary medicines, in particular to a preparation method of a medicinal herb preparation for treatment of live pig viral enteritis. The medicinal herb preparation is prepared from the components in parts by weight: 9-12 parts of fructus forsythiae, 8-10 parts of herba taraxaci, 8-11 parts of flos lonicerae, 6-9 parts of mangnolia officinalis, 7-9 parts of fried semen armeniacae amarae, 5-7 parts of gypsum fibrosum, 6-8 parts of radix isatidis, 4-6 parts of lotus petiole, 5-7 parts of herba houttuyniae, 5-7 parts of Chinese granesbill, 3-5 parts of rhizoma coptidis, 2-4 parts of rhodiola, 3-5 parts of fried fructus crataegi, 3-5 parts of licorice roots northwest origin and proper amount of deionized water. According to the preparation method of the medicinal herb preparation, the raw materials of the medicinal herb preparation for the treatment of the live pig viral enteritis are prepared, the medicinal herb preparation effectively prevents and treats the pig viral enteritis, side effects are avoided, the curative effect is fast, at the same time, the gastrointestinal function can be regulated, appetite of pigs is increased, in addition, preparation is simple, and the medicinal herb preparation is worthy of mass production and use.

The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a preparation device and method of a traditional Chinese medicine fermentation preparation for treating duck viral enteritis. The preparation device comprises a fermentation tank, the inner wall of the fermentation tank is sleeved with a partition plate, a motor is fixedly installed on the inner wall of the bottom of the fermentation tank, and a transmission shaft is fixedly installed on an output shaft of the motor; the tail end of the transmission shaft rotationally penetrates through the partition plate and is fixedly provided with two stirring blades, openings are formed in the surfaces of the stirring blades, the stirring blades are uniformly sleeved with a plurality of same spiral pipes, each spiral pipe comprises a pipe body and wedge-shaped blocks, and a plurality of wedge-shaped blocks are fixedly installed on the inner wall of each pipe body. Openings are formed in the stirring blades, and spiral pipes are arranged on the stirring blades, so that medicine powder can be fully stirred during stirring, and the situation that in the stirring process of the medicine powder, due to inertia of the medicine powder, the medicine powder and ferment powder are relatively static, and the stirring efficiency is reduced is prevented.

The invention discloses drug for the treatment of abdominal pain and diarrhea and belongs to the field of TCM. The drug comprises pharmaceutical effective ingredients, including Chinese granesbill, turtle shell, Eryngium foetidum herb, multibract raspberry root, cutch, birch bark, radish seed, Asiatic pennywort herb, common turczaninowia herb, Russian olive fruit, purple flower holly leaf, coastal heritiera seed, chalk, cucubalus root, Yunnan Pistache leaf, and quince. The drug has the effects of regulating qi to alleviate pain, clearing away heat and eliminating dampness, soothing the liver to dispel melancholy and tonifying the spleen to cure diarrhea, and has the significant effect of the diarrhea, watery stool, abdominal pain and poor appetite caused by damp-heat accumulation and the viral enteritis and non-infectious diarrhea caused by rotavirus. The drug has the advantages that the preparation process is simple, curative effect is good and fast, the drug is convenient to take and available for treating both symptoms and root causes, and the drug is neutral in property and has no toxic and side effects; the drug has good effects for the treatment of inappetence, nausea and vomiting, abdominal distension and diarrhea, and dyspepsia.

The invention discloses a traditional Chinese medicine capsule for treating infantile viral enteritis. The traditional Chinese medicine capsule is prepared from the following medicinal materials in parts by weight: 10 parts of bitter herb, 10 parts of pistacia weinmannifolia, 8 parts of chaenomeles speciosa nakai, 6 parts of pericarpium papaveris, 10 parts of feronia limonia, 6 parts of Mongolian oak bark and 10 parts of orobanche. Clinical experiments prove that the remarkable effect of treating the infantile enteritis is achieved by selecting the appropriate medicinal materials and the appropriate proportion; the treatment effect is definite, the cure time is short, and the capsule is safe and free from toxic and side effects.

The invention discloses a Chinese herbal medicine for preventing and treating pig viral enteritis. The medicine is prepared from, by weight, 9-12 parts of broomjutre sida herb, 8-13 parts of ficus tikoua, 7-9 parts of lotus petiole petioles, 5-7 parts of poria cocos, 3-5 parts of mangnolia officinalis, 4-6 parts of lucid ganoderma, 5-7 parts of codonopsis pilosula, 4-6 parts of walking spleenwort, 5-7 parts of roots of kudzu vine, 3-5 parts of aristolochia impressinervis C.F.Liang, 4-6 parts of geranium strictipes, 5-7 parts of gynostemma pentaphylla, 3-5 parts of kadsura longepedunculata, 1-3 parts of rhizoma polygoni paleacei, 3-5 parts of purslane, 2-4 parts of ash bark, 3-5 parts of radix paeoniae alba, 5-7 parts of honeysuckle flowers, 4-6 parts of Chinese yam, 3-5 parts of peanut oil, 2-4 parts of fried hawthorn fruits, 4-6 parts of bitter gourd, 1-3 parts of dried ginger, 3-5 parts of unprocessed licorice roots and 2-4 parts of borneol. Pure Chinese herbal medicines are adopted as raw materials, the medicine sources are wide, materials are easy to obtain, and the cost is low. Due to the synergistic effect of medicine effects, the Chinese herbal medicine has the effects of activating blood, promoting circulation of qi, tonifying the spleen and the stomach, eliminating dampness, checking diarrhea, promoting digestion to eliminate stagnation and clearing away heat and toxic materials; after a pig takes the medicine, the effect is rapid to achieve, the curative effect is good, and the medicine is safe, non-toxic, capable of effectively preventing and treating pig viral enteritis, adjusting the intestines and stomach functions and enhancing the pig appetite and worthy of mass production and application.

The invention discloses an immunization method of high-yielding laying hens. When the selected laying hens grow to be 1 day old, administrating marek's disease and viral enteritis bivalent yolk fluid to the selected laying hens through the subcutaneous injection, when the selected laying hens grow to be 6-7 days old, inoculating ND-IB living vaccines and ND-IB trivalent oil emulsion vaccines to the selected laying hens, when the selected laying hens grow to be 10-15 days old, inoculating escherichia coli and avian influenza vaccines to the selected laying hens, when the selected laying hens grow to be 25-30 days old, inoculating bursal disease vaccines to the selected laying hens, when the selected laying hens grow to be 40-50 days old, inoculating infectious coryza vaccines to the selected laying hens, and when the selected laying hens grow to be 40-50 days old, inoculating recombinant avian influenza virus H5 subtype bivalent inactivated vaccines to the selected laying hens. According to the immunization method, the corresponding vaccines are used in a pertinent manner aiming at the different diseases possibly caused to the laying hens at different growth stages, so that the occurrence of the diseases of the laying hens can be effectively controlled, the laying hens have a good immunity function at different growth stages, further, the survival rate and the health of the laying hens are guaranteed, and finally, the financial loss caused to the farmer due to the fact that the laying hens are infected with diseases is effectively avoided.

Rotaviral enteritis mostly occurs in autumn, is mainly infected through the digestive tract or the respiratory tract and has the major clinical manifestations: aches, dizziness, diarrhea, and hypodynamia symptoms. People suffering severe rotaviral enteritis even have isotonic dehydration and electrolyte disorder symptoms. Western medicines have poor treatment effects clinically. The invention provides a traditional Chinese medicine for treating viral enteritis to solve the above problem. The traditional Chinese medicine science considers that the basic pathology of the viral enteritis is indigestion and coldness induced spleen deficiency and middle jiao deficiency coldness. The traditional Chinese medicine is mainly used for invigorating spleen, clearing heat and stopping diarrhea, and fundamentally solves the problem of poor clinic treatment effect of the western medicines.

The invention discloses a Chinese herbal preparation for treating infant viral enteritis. The infant viral enteritis belongs to a diarrhea scope in the traditional Chinese medical science, and the diarrhea is caused by transformation failure of the spleen and the stomach because of exogenous pathogenic factors or internal injury diets, so that essence fluid is easily lost after the disease is occurred. According to the Chinese herbal preparation, Chinese medicinal herbs of sphenomeris, agiopteris roots, amur corktree barks, poria cocos, silverweed cinquefoil roots, nutmeg, bird cherry, bighead atractylodes rhizome, glass grass fruits, dried guava and licorice roots are selected and decocted with water to prepare decoction of herbal medicine. The clinical test in hospital shows that the total efficiency is 96.7 percent, and the efficiency is obviously superior to that of a control group; and meanwhile, the treatment effect of excrement rotavirus antigen turn rate is higher than that of a control group after the treatment is carried out for 72 hours, so that the Chinese herbal preparation is worthy of clinical popularization and application.

The invention provides a recombinant duck viral enteritis virus vaccine for expressing secretory duck Tembusu virus E protein, and a construction method and application thereof. The collection number is CCTCC V201214, and the name is rDEV-TE-tPAS. By using a recombinant clone technique, a gene segment SV40-E-tPA comprising an SV40 promoter, a duck Tembusu virus E protein and a tPA (Tissue plasminogen activator) signal peptide sequence is inserted into a spacer between the US7 and US8 genes of the duck viral enteritis virus to construct a cosmid in which the SV40-E-tPA expression frame is inserted between the US7 and US8 genes, thereby obtaining the recombinant duck viral enteritis virus vaccine CCTCC V201214 for expressing secretory duck Tembusu virus E protein. The invention also provides a method for constructing a recombinant duck viral enteritis virus vaccine strain and application of the recombinant duck viral enteritis virus vaccine strain in preparing a vaccine for preventing infectious diseases caused by duck viral enteritis virus and duck Tembusu virus.

The present invention discloses a traditional Chinese medicinal herb preparation, which is prepared with ginseng, Chinese caterpillar fungus, lucid ganoderma, velvet antler, tortoise shell, beef tendon, astragalus root, divaricate saposhnikovia root, kudzuvine root, Indian buead, costus root, semen plantaginis, fried rhizoma atractylodis macrocephalae, medcinal evodia fruit, rhizoma zingiberis, malaytea scurfpea fruit , fossilizid, oyster, hawthorn fruit, goosegrass herb, indigowoad root , eucalyptus robusta smith, common andrographis herb, baical skullcap root, golden thread, borneol and liquoric root. The traditional Chinese medicinal herb preparation can promote infantile digestive absorption, stimulate appetite, protect health, enhance the immunologic function and disease-resisting capability of infants and prevent and treat infantile virus enteritis, and the effective rate can reach 95 percent and 98.6 percent in preventing and curing infantile virus enteritis. The traditional Chinese medicinal herb preparation, which has the advantages of safety, non-toxicity and no generation of drug fastness, is an ideal traditional Chinese medicinal herb preparation for preventing and curing infantile virus enteritis.

The invention provides a method for quickly detecting duck viral enteritis by real-time fluorescence quantitative PCR. The method selects a gene fragment of duck viral enteritis UL31 to design a primer and a probe, and order-checking results on an amplified fragment proves that homologies of the amplified fragment and the gene fragment are both 100 percent without a cross reaction, thereby eliminating problems of virus variation and false negative caused by region factors, and improving detectable positive rate and compatibility of the method. The method constructs a quantitative positive quality control standard (recombinant plasmid pMD18-T-DEV76bp), establishes a standard curve of fluorescence quantitative detection, and can minimally detect 2.1*10 degrees copy / [mu]liter. The assembled standard kit reacts under a standard reaction condition, and determines according to accurate quality control standard and determination standard. The method has the advantages of strong specificity, high sensibility, safe quantitative positive quality control stand, simple operation and low cost, is applicable to large-scale quarantine inspection, port quarantine inspection, epidemiological investigation and laboratory quick diagnose of field duck viral enteritis.

The invention provides a recombinant duck viral enteritis virus vaccine for expressing secretory duck Tembusu virus M / E protein, and a construction method and application thereof. The collection number is CCTCC NO:V201215, and the name is rDEV-TME-tPAS. By using a recombinant clone technique, a gene segment rDEV-TME-tPAS comprising an SV40 promoter, duck Tembusu virus M and E proteins and a tPA (Tissue plasminogen activator) signal peptide sequence is inserted into a spacer between the US7 and US8 genes of the duck viral enteritis virus to construct a cosmid in which the rDEV-TME-tPAS expression frame is inserted between the US7 and US8 genes, thereby obtaining the recombinant duck viral enteritis virus vaccine CCTCC V201215 for expressing secretory duck Tembusu virus M / E protein. The invention also provides a method for constructing a recombinant duck viral enteritis virus vaccine strain and application of the recombinant duck viral enteritis virus vaccine strain in preparing a vaccine for preventing infectious diseases caused by duck viral enteritis virus and duck Tembusu virus.