The invention relates to a chromatographic method for separating and purifying high-purity
porcine circovirus Cap proteins. The chromatographic method comprises the following steps: (S1)
grinding and breaking
insect cells expressing the
porcine circovirus Cap proteins, extracting supernatant, and filtering by virtue of a filter membrane with a
pore diameter of 0.45 micron, so as to obtain an original sample solution containing the
porcine circovirus Cap proteins; (S2) carrying out weak
anion exchange chromatography on the original sample solution, so as to obtain a first purified solution; (S3) carrying out strong cation exchange exchange
chromatography on the first purified solution, so as to obtain a second purified solution; and (S4) carrying out gel
filtration chromatography on the second purified solution, so as to obtain a high-purity porcine
circovirus Cap
protein solution. By virtue of the chromatographic method,
DNA, pigments and host
cell proteins in a rough original sample solution obtained from the
insect cells expressing the porcine
circovirus Cap proteins can be removed, and the porcine
circovirus Cap proteins are greatly concentrated so as to obtain the high-purity and high-concentration porcine circovirus Cap proteins, so that the
immunogenicity and
reactogenicity of the Cap proteins are improved, and side effects are reduced.