Chromatographic method for separating and purifying high-purity porcine circovirus Cap proteins

Abstract

The invention relates to a chromatographic method for separating and purifying high-purity porcine circovirus Cap proteins. The chromatographic method comprises the following steps: (S1) grinding and breaking insect cells expressing the porcine circovirus Cap proteins, extracting supernatant, and filtering by virtue of a filter membrane with a pore diameter of 0.45 micron, so as to obtain an original sample solution containing the porcine circovirus Cap proteins; (S2) carrying out weak anion exchange chromatography on the original sample solution, so as to obtain a first purified solution; (S3) carrying out strong cation exchange exchange chromatography on the first purified solution, so as to obtain a second purified solution; and (S4) carrying out gel filtration chromatography on the second purified solution, so as to obtain a high-purity porcine circovirus Cap protein solution. By virtue of the chromatographic method, DNA, pigments and host cell proteins in a rough original sample solution obtained from the insect cells expressing the porcine circovirus Cap proteins can be removed, and the porcine circovirus Cap proteins are greatly concentrated so as to obtain the high-purity and high-concentration porcine circovirus Cap proteins, so that the immunogenicity and reactogenicity of the Cap proteins are improved, and side effects are reduced.

Description

technical field [0001] The invention relates to the field of animal vaccines, and more particularly relates to a chromatography method for separating and purifying high-purity porcine circovirus Cap protein. Background technique [0002] Porcine circovirus is the pathogen of postweaning multisystemic wasting syndrome (Postweaning multsystemic wasting syndrome, PMWS), which can cause progressive weight loss, cough, dyspnea, diarrhea, pale or yellow skin, enlarged lymph nodes, Kidney pale edema and other symptoms. At the same time, the virus can also cause a certain degree of immunosuppression, which can easily cause secondary or concurrent infections of other diseases. Since clinical porcine circovirus was first discovered in Canada in 1991, the disease has caused huge economic losses to the swine industry all over the world. [0003] The Cap protein of porcine circovirus is the main structural protein of porcine circovirus, which can self-assemble into viral particles and ...

AI Technical Summary

Problems solved by technology

However, the effects achieved by these methods are not significant, and these methods involve changes in the production process, and the immunogenicity and reactogenicity of the vaccine may also be reduced to varying degrees.

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