Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof

A technology of PPR and protein antigens, applied in the fields of bioinformatics and immunology, can solve problems such as unfavorable PPR global eradication plan, vaccine thermal stability and virulence reversion

Active Publication Date: 2017-09-29
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main prevention method of the disease is the attenuated vaccine immunization, but the vaccine has problems such as thermal stability and anti-virulence, and it is not conducive to the global eradication plan of Peste des petits ruminants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof
  • Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof
  • Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0056] A kind of Peste des petits ruminants virus HN protein epitope peptide of the present invention, the aminoacid sequence of described epitope peptide is H362: 362 EANWVVPSTDVRDL 375 .

[0057] The method for determining and preparing the antigenic epitope of the present invention comprises the following steps:

[0058] Calculation simulation:

[0059] Step 1: Use molecular simulation software to construct the 3D structure of the virtual HN protein head: Use Discovery Studio V4.5 to perform homology modeling on the two target proteins——PPRV HN of wild strain and PPRV HN protein of vaccine strain; among them, PPRVHw, GenBank The accession number is FJ905304; PPRV Hv, GenBank accession number: X74443;

[0060] Step 2, search for templates: search the PDB database (www.pdb.org), find the structure of homologous proteins resolved through experiments, and select the protein structure with a sequence identity of more than 30% and a relatively long sequence as the template str...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an epitope peptide H362 of an HN protein in PPRV, and determination, a preparation method and application thereof. The amino acid sequence of the epitope peptide is H362: <362>EANWVVPSTDVRDL<375>. The invention detects reactogenicity of a monoclonal antibody and PPRV and specificity of the monoclonal antibody; according to detection results, the monoclonal antibody has good reactogenicity to rPPRV-HN-F protein; immunoinformatic technology is cooperatively used for predicating the B cell epitope of the HN protein; an aminated ELISA plate is employed for detecting candidate epitopes and the monoclonal antibody 10E3, and the epitope peptide H362 corresponding to 10E3 is determined; and determination of the epitope peptide lays a theoretical foundation for preparation of epitope vaccine antigens and diagnostic reagent antigens for PPRV.

Description

technical field [0001] The invention belongs to the technical fields of bioinformatics and immunology, and in particular relates to an antigenic epitope peptide H362 of HN protein of Peste des petits ruminants virus and its determination, preparation method and application. Background technique [0002] Peste des petits ruminants virus (PPRV) belongs to the genus Morbillivirus and is the pathogen that causes acute infectious diseases of small ruminants such as goats and sheep. The disease is characterized by high morbidity and mortality, and is recognized by the World Organization for Animal Health (OIE) Listed as a legally notifiable animal infectious disease, my country classifies it as a Class I animal disease. Peste des petits ruminants virus was initially reported to only infect goats and sheep, but in recent years, cases of interspecies transmission of the virus have been continuously reported. At present, the disease is mainly distributed in Africa and Asia, and is thr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/12A61K39/165A61P31/14G01N33/569G01N33/68
CPCA61K39/12A61K2039/552C07K14/005C12N2760/18322C12N2760/18334G01N33/56983G01N33/6854G01N2469/20
Inventor 窦永喜梁忠祥朱学亮蒙学莲张志东才学鹏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com