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32results about How to "Strong green fluorescence" patented technology
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Epitope peptide H362 of HN protein in peste des petits ruminants virus (PPRV), and determination, preparation method and application thereof
ActiveCN107216372AStrong green fluorescenceGood reactogenicitySsRNA viruses negative-senseViral antigen ingredientsF proteinHN Protein
The invention relates to an epitope peptide H362 of an HN protein in PPRV, and determination, a preparation method and application thereof. The amino acid sequence of the epitope peptide is H362: <362>EANWVVPSTDVRDL<375>. The invention detects reactogenicity of a monoclonal antibody and PPRV and specificity of the monoclonal antibody; according to detection results, the monoclonal antibody has good reactogenicity to rPPRV-HN-F protein; immunoinformatic technology is cooperatively used for predicating the B cell epitope of the HN protein; an aminated ELISA plate is employed for detecting candidate epitopes and the monoclonal antibody 10E3, and the epitope peptide H362 corresponding to 10E3 is determined; and determination of the epitope peptide lays a theoretical foundation for preparation of epitope vaccine antigens and diagnostic reagent antigens for PPRV.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Method for establishing and identifying cynoglossus semilaevis testis cell line
InactiveCN102304491AFast growthHigh passaging frequencyMicrobiological testing/measurementMicroorganism based processesBottleCynoglossus semilaevis
The invention relates to a method for establishing and identifying a cynoglossus semilaevis testis cell line, which comprises the following steps: adopting a MEM (Minimum Essential Medium), adding 20 percent of fetal calf serum, 0.1 percent of B-mercaptoethanol, 2 ng / ml of human alkaline fibroblastic growth factors, 1 ng / ml of leukemia inhibiting factors, 1 nmol of sodium pyrurate and 1 nmol of glutamine and preparing into a complete medium; inoculating a tissue block into a culture bottle, overturning and inversely placing the bottle to culture for 3 h and positively placing the culture bottle after the tissue block is attached onto a wall, so that the tissue block is soaked into the culture medium for culture. A trypsin digestion method is adopted for subculturing cells. One cynoglossus semilaevis testis cell line is established by adopting the above method and is subcultured to fiftieth generations. Meanwhile, the invention also provides a method for identifying a chromosome of the established cell line and the level of a molecular marker and a method for identifying the sensitivity of the cell line to related viruses. The method for establishing the cynoglossus semilaevis testis cell line can be used for culturing other fish gland cells and establishing the cell line, thereby having wider promotion and application prospect.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Construction method of bastard halibut brain cell system
InactiveCN103789263ANormal growth curveStrong green fluorescenceMicroorganism based processesNervous system cellsCulture fluidToxicology studies
The invention discloses a method for establishing a bastard halibut brain tissue-derived cell system. The method comprises brain tissue primary culture, subculture, cryopreservation, recovery and identification, wherein cell culture fluid adopted is obtained by adding fetal calf serum and human basic fibroblast growth factor to basal cell culture fluid. The established bastard halibut brain cell system is represented as an epithelioid-like astrocytic cell, which can support continuous passage to provide a great amount of bastard halibut brain cells and can be directly used for research of bastard halibut functional gene. The construction method disclosed by the invention not only is expected to serve as a platform for theoretical research of bastard halibut molecule cellular level but also can be used as an ideal material for research on fish hemadenology, reproductive biology and environmental internal-secretion interfering-substance toxicology.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) visual kit for detecting Japanese B encephalitis virus and application of kit
InactiveCN102399909ALow costReduce use costMicrobiological testing/measurementBetaineJapanese B Encephalitis Virus
The invention discloses a reverse transcription loop-mediated isothermal amplification (RT-LAMP) visual kit for detecting Japanese B encephalitis virus and application of the kit. The visual kit contains a primer group and color development substances, wherein primers have sequences shown as SEQ ID NO.1-4, and the color development substances are calcein and manganese chloride. A using method of the kit comprises the following steps of: preparing an RT-LAMP system containing AMV reverse transcriptase, a 1 time reaction buffer solution, strand displacement DNA polymerase, a dNTP mixture, betaine, calcein, manganese chloride, MgSO4, a forward inner primer (FIP), a backward inner primer (BIP) group, an F3 primer, a B3 primer and RNA of a sample to be detected; performing thermostatic reaction on the reaction system at the temperature of between 61 and 65 DEG C, and inactivating; and judging a result under natural light or ultraviolet or natural light and ultraviolet, wherein if the reaction product is green under the natural light, the sample to be detected contains the Japanese B encephalitis virus; and if the reaction product represents obvious green fluorescence under the ultraviolet, the sample to be detected contains the Japanese B encephalitis virus.
Owner:SOUTH CHINA AGRI UNIV
Copper nano-cluster fluorescent material and preparation method thereof
ActiveCN107603604AThe preparation method is simple and environmentally friendlySimple and fast operationNanotechnologyLuminescent compositionsQuantum yieldLength wave
The invention discloses a copper nano-cluster fluorescent material and a preparation method thereof. Copper nanoparticles protected by ascorbic acid are used as a precursor to synthesize the copper nano-cluster fluorescent material from top to bottom. Ammonia water is used as an etching agent to control the formation of a copper nano-cluster. The preparation method of the novel copper nano-clusterfluorescent material, provided by the invention, has the advantages of simplicity in preparation, environment protection, rapidness, low price and the like. The prepared copper nano-cluster has strong green fluorescent light; the maximum emission wavelength is 498nm and the yield of quanta is 6.63 percent.
Owner:FUJIAN MEDICAL UNIV
Lead-free all-inorganic indium-based zero-dimensional perovskite nanocrystal and manufacturing method thereof
PendingCN113072933AStrong green fluorescenceHigh fluorescence quantum yieldNanoopticsLuminescent compositionsQuantum yieldIndium
The invention relates to a lead-free all-inorganic indium-based zero-dimensional perovskite nanocrystal and a manufacturing method thereof, the chemical formula of the lead-free all-inorganic indium-based zero-dimensional perovskite nanocrystal is Rb3nCs3-3nInX6, the Rb3nCs3-3nInX6 is doped with antimony, the Rb3nCs3-3nInX6 is reacted to form Rb3nCs3-3nInX6: Sb, X is Cl or Br, and n is more than or equal to 0 and less than or equal to 1. The corresponding nanocrystal is obtained through the steps of raw material adding, variable-temperature treatment, centrifugal treatment and the like. According to the invention, a small amount of antimony element is used for doping, so that the prepared lead-free all-inorganic indium-based perovskite nanocrystal has very strong green fluorescence, the fluorescence quantum yield is up to 45.3%, and the lead-free all-inorganic indium-based perovskite nanocrystal is not only suitable for electroluminescence in a light-emitting diode, but also suitable for a fluorescent layer of a green light-emitting diode, and has a very good photoelectric application prospect.
Owner:NANJING UNIV OF SCI & TECH
RT-LAMP (reverse transcription loop-mediated isothermal amplification) nucleic acid detection primers and kit of Hantaan viruses
InactiveCN104946799ALow costReduce use costMicrobiological testing/measurementMicroorganism based processesNucleic acid detectionMicrobiology
The invention discloses RT-LAMP (reverse transcription loop-mediated isothermal amplification) nucleic acid detection primers and a kit of Hantaan viruses and in particular relates to a group of primers for detecting Hantaan viruses, a kit containing the primers and a detection method of the Hantaan viruses. The primers comprise base sequences shown in a sequence table SEQ ID No.1 to a sequence table SEQ ID No.6. The kit has high sensitivity and strong specificity, is low in cost and is simple and convenient to operate.
Owner:BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT +1
Peste des petits ruminant virus HN protein epitope peptide as well as determination and preparation method and application thereof
PendingCN107033225AStrong green fluorescenceImprove responseSsRNA viruses negative-senseViral antigen ingredientsProtein targetHN Protein
The invention relates to peste des petits ruminant virus HN protein epitope peptide. The amino acid sequences of the epitope peptide are as follows: H123: <123>KFLNPDREYDFRDLR<137>, or / and H185: <185>GTGCLGRTVTRA<196>, or / and H487: <487>IRGPRGRCH<495>, or / and H569: <569>ECFPWYHKVWCYHDCLI<585>. B cell epitopes of a target protein are predicted by virtue of multiple immunoinformatic software, the different predicted epitopes are respectively artificially synthesized, the reactogenicity of the predicted epitopes is verified by virtue of an indirect ELISA method, an aminated ELISA Plate is coated with different polypeptides, and the reactogenicity with an antibody of HN protein is detected, and therefore, the B cell epitopes of the PPRV HN protein are authenticated.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Peste des petits ruminant virus F protein epitope peptide as well as determination and preparation method and application thereof
ActiveCN107033226AStrong green fluorescenceImprove responseSsRNA viruses negative-senseViral antigen ingredientsProtein targetF protein
The invention relates to peste des petits ruminant virus F protein epitope peptide. The amino acid sequences of the epitope peptide are respectively as follows: F176: <176>DYINNELVPSVHRMSCEL<193>, or / and F347: <347>MSPLLQECFRGSTKS<361>, or / and F391: <391>CKCYTTETVINQDPDKLL<408>, or / and F417: <417>TVINQDPDKGPVGSREYPD<435>, or / and F430: <430>SREYPDSVYLH<440>, or / and F502: <502>VSLGLVTLICCCKGRCRNK<520>. B cell epitopes of a target protein are predicted, the different predicted epitopes are respectively artificially synthesized, the reactogenicity with an antibody of F protein is detected, and therefore, the B cell epitopes of the PPRV F protein are authenticated.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Gold ion probe, and preparation method and application thereof
ActiveCN104710975AStrong green fluorescenceHigh detection sensitivityOrganic chemistryFluorescence/phosphorescenceFluoresceinHigh selectivity
The invention discloses a gold ion fluorescence probe, a preparation method and an application thereof. The fluorescence probe has high selectivity and high sensitivity, can be used for detecting gold ions. The gold ion fluorescence probe is a 4,5-dihydrazine pyridyl fluorescein derivative shown in a formula I, the preparation method comprises the following steps of reacting 4,5-fluorescein bi-aldehydes and hydrazinopyridines in an ethanol solution to generate the dihydrazine pyridyl fluorescein derivative which can be taken as the gold ion fluorescence probe.
Owner:NANJING UNIV OF TECH
Method for detecting quercetin through fluorescent micro-probe and application
ActiveCN113736091AEnhanced system fluorescenceStrong specificityFluorescence/phosphorescenceLuminescent compositionsAluminum IonQuercitrin
The invention discloses a method for detecting quercetin through a fluorescent micro-probe and application. According to the invention, curcumin is taken as a target and phosphonitrilic chloride trimer is taken as a linking group to form a polycyclotriphosphazene co-curcumin fluorescent microsphere; the fluorescent micro-probe is formed on the basis of complexing of the fluorescent microsphere and an aluminum ion; and by virtue of good coordination capability of the quercetin and the metal ion, the quercetin is combined with the aluminum ion in the fluorescent micro-probe to release a fluorescence signal so as to enhance the fluorescence of a system, so rapid detection of the quercetin is realized. The fluorescent micro-probe is a polyphosphazene hyperbranched micron-sized fluorescent micro-probe, fluorescence signal stability and sensing performance under complex background interference are improved by constructing a curcumin-metal ion-quercetin micron-sized reactor, the lowest detection limit of quercetin micromolecules is 2.32 * 10<-8> mol / L, rapid response within 1 min is achieved, high sensitivity is realized, anti-interference capability is strong, identification is rapid, and a detection result is accurate.
Owner:SHANGHAI UNIV OF ENG SCI
Terbium diphenylamine carbonyl benzoic acid rare earth complex as well as preparation method and application thereof
ActiveCN113201001AThe synthesis process is simpleLow costGroup 3/13 organic compounds without C-metal linkagesOrganic chemistry methodsBenzoic acidFluoProbes
The invention provides a terbium diphenylamine carbonyl benzoic acid rare earth complex, which is synthesized by taking 2-diphenylamine carbonyl benzoic acid and terbium nitrate as raw materials through water bath heating and a one-pot method. The preparation method has the advantages of simple synthesis process, low cost, easy control of chemical components, good repeatability, high yield and the like. The terbium diphenylamine carbonyl benzoic acid rare earth complex is good in fluorescence intensity and monochromaticity, and under a three-purpose ultraviolet lamp, when the excitation wavelength is 254 nm, the material emits strong green fluorescence. The terbium diphenylamine carbonyl benzoic acid rare earth complex can be used as a fluorescent probe to identify dichromate ions (Cr2O7<2->) harmful to the environment, and has high selectivity.
Owner:HENGYANG NORMAL UNIV
Green fluorescent diamine-oxazoline zinc complex as well as preparation method and application thereof
ActiveCN112079790AImprove stabilityStrong green fluorescenceOrganic chemistryLuminescent compositionsEthyl groupRing-opening polymerization
The invention provides a green fluorescent diamine oxazoline zinc complex as well as a preparation method and application thereof, and belongs to the technical field of catalysis of luminescent materials. The complex can be obtained by direct reaction and purification of diamine oxazoline ligands containing different substituent groups and diethyl zinc, and the complex is high in yield and purity.The diamine-oxazoline zinc complex shows green fluorescence by taking 380nm as an excitation wavelength, the maximum emission wavelength is 491-495nm, and the fluorescence lifetime of the diamine oxazoline zinc complex is 10-10.75 microseconds. Moreover, the diamine oxazoline zinc complex can be directly used for catalyzing the ring-opening polymerization reaction of cyclic lactone and has relatively high catalytic activity, so that a series of high polymer materials with specific structures are obtained.
Owner:DALIAN UNIV OF TECH
Carbon quantum dot two-photon fluorescent dye for ultrafast cell staining
PendingCN114736675AGood biocompatibilityGood imaging effectNanoopticsFluorescence/phosphorescenceFluorescent imagingLight source
The invention discloses a carbon quantum dot two-photon fluorescent dye for ultrafast cell staining. The carbon quantum dot two-photon fluorescent dye is prepared by the following steps: 1) preparing a carbon quantum solution by taking m-phenylenediamine as a precursor and adopting a one-step solvothermal method; and 2) separating a green fluorescent component from the carbon quantum prepared in the step 1) by adopting silica gel column chromatography, namely the carbon quantum dot two-photon fluorescent dye. The absolute quantum efficiency of the carbon quantum dot two-photon fluorescent dye for ultrafast cell staining provided by the invention reaches up to 58.65%, and the carbon quantum dot two-photon fluorescent dye shows good biocompatibility and shows an excellent imaging effect even at a low concentration; the fluorescent probe can enter cells within 10 seconds, so that a solid foundation is provided for rapid fluorescence imaging; in addition, the G-CDs can also be used for two-photon imaging, a near-infrared excitation light source is used, and the G-CDs also shows strong green fluorescence and shows excellent imaging performance in 4T1 cells.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Hypochlorite ion fluorescent probe, preparation method and application thereof
ActiveCN104710976BStrong green fluorescenceHigh detection sensitivityOrganic chemistryHydrazone dyesHypochloriteAlcohol
The invention discloses a hypochlorite ion fluorescent probe, a preparation method and its application, which can be used for detection of hypochlorite ion content in the environment and fluorescence development and content detection of hypochlorite distribution in biological samples. The fluorescent probe of the present invention only has a fluorescence response to hypochlorite, and has no reaction to various other reactive oxygen species (ROS) including H2O2, NO·,·O2‑, ONOOˉ, ROO·, 1O2,·OH, and has good Selectivity and specificity; the probe preparation process is simple and easy to produce on a large scale. The structure of the probe of the present invention is shown in Formula I; its preparation method is as follows: reacting fluorescein monoaldehyde and hydrazinopyridine in an ethanol solution to generate a hypochlorite ion fluorescent probe.
Owner:NANJING TECH UNIV
Fusarium moniliforme SiC quantum dot fluorescence labeling method
InactiveCN105779560AEvenly distributedRealize dynamic monitoringMicrobiological testing/measurementLuminescent compositionsFluorescenceCytotoxicity
The invention relates to a fusarium moniliforme SiC quantum dot fluorescence labeling method and particularly relates to a fluorescence labeling method for fusarium moniliforme SiC in different growth stages by cytotoxicity-free aqueous phase silicon carbide quantum dots, and a long-time-period fluorescence imaging technology for living cells. The method comprises the following steps of preparing a mixed solution of slant strain fusarium moniliforme and a PD culture medium; preparing a mixed culture medium of a mycete liquid-SiC quantum dot aqueous phase solution from the mixed solution and an SiC quantum dot aqueous phase solution at a volume ratio of 3:1; performing constant-temperature shaking culture on the mixed culture medium at a temperature of 28 DEG C and a speed of 200r / min for 3-10 days, thereby obtaining a fusarium moniliforme SiC quantum dot fluorescent mark; applying the mark to seedling root dip dyeing, thereby realizing dynamic monitoring, tracing and fluorescence imaging of a process that the fusarium moniliforme infects plant roots. The method is simple and effective, is low in cost and is capable of realizing fluorescence labeling of the fusarium moniliforme in different periods.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Luminous silver nanocluster and preparation method and application thereof
ActiveCN113369489AImprove luminosityEasy to makeMaterial nanotechnologyFluorescence/phosphorescenceAqueous solubilityAqueous solution
The invention relates to a luminous silver nanocluster and a preparation method and application thereof. The preparation method comprises the following steps: mixing 2-14 mmol / L of a 2-sulfydryl-5-benzimidazole sodium sulfonate aqueous solution and 1 mmol / L of a silver nitrate solution in an isovolumetric manner to obtain a mixed solution; and uniformly stirring the mixed solution, adding enough 0.1 mol / L ascorbic acid, uniformly stirring, adding a NaOH solution to adjust the pH value to 7, controlling the heating temperature to be 40-100 DEG C, refluxing for 2-24 hours, cooling, taking out, dialyzing and purifying to obtain the luminous silver nanocluster. The preparation process is simple, reaction conditions are simple, the method is environment-friendly, the prepared luminous silver nanocluster has the advantages of good water solubility, good stability, high quantum yield and the like and can be applied to high-sensitivity and high-selectivity Cu2+ recognition and detection, the detection process is simple, convenient and rapid, and the detection result is accurate.
Owner:SHANXI UNIV
A Chinese Mortierella hirsutella sinensis transformed bacterial strain expressing green fluorescent protein and its preparation method
ActiveCN107418965BSimple materialConvenient for molecular biology researchFungiMicroorganism based processesBacterial strainFunctional genes
Owner:成都图径生物科技有限公司
Gold ion probe and its preparation method and application
ActiveCN104710975BStrong green fluorescenceHigh detection sensitivityOrganic chemistryFluorescence/phosphorescenceFluoresceinHigh selectivity
The invention discloses a gold ion fluorescence probe, a preparation method and an application thereof. The fluorescence probe has high selectivity and high sensitivity, can be used for detecting gold ions. The gold ion fluorescence probe is a 4,5-dihydrazine pyridyl fluorescein derivative shown in a formula I, the preparation method comprises the following steps of reacting 4,5-fluorescein bi-aldehydes and hydrazinopyridines in an ethanol solution to generate the dihydrazine pyridyl fluorescein derivative which can be taken as the gold ion fluorescence probe.
Owner:NANJING TECH UNIV
Guanidine hydrochloride/6-aza-2-thiothymine-gold nanocluster and preparation method thereof
ActiveCN105860963BEasy to makeStrong green fluorescenceLuminescent compositionsQuantum yieldCombinatorial chemistry
The invention discloses a guanidine hydrochloride / 6-aza-2-sulfo-thymine-gold nano cluster and a preparation method thereof. A 6-aza-2-sulfo-thymine-gold nano cluster is adopted as a precursor, guanidine hydrochloride is adopted as a ligand rigidization agent, and a superbright water-soluble gold nano cluster fluorescent material is obtained through synthesis. The preparation method of the novel gold nano cluster fluorescent material has the advantages of being fast to prepare, simple, environmentally friendly and the like. The synthesized guanidine hydrochloride / 6-aza-2-sulfo-thymine-gold nano cluster emits strong green fluorescence (the maximum emitting wave length is 530 nm), and the prominent advantages of being high in quantum yield (71%), wide in excitation spectrum, narrow in emitting spectrum, good in water solubility and the like are achieved.
Owner:FUJIAN MEDICAL UNIV
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) visual kit for detecting Japanese B encephalitis virus and application of kit
InactiveCN102399909BLow costReduce use costMicrobiological testing/measurementBetaineJapanese B Encephalitis Virus
Owner:SOUTH CHINA AGRI UNIV
A kind of fluorescent probe for detecting palladium ion, preparation method and application
ActiveCN106749093BHigh detection sensitivityGood selectivity and specificityOrganic chemistryFluorescence/phosphorescenceFluorescenceChemistry
The invention discloses a fluorescent probe for detecting palladium ions, and provides a preparation method and application of the fluorescent probe at the same time. The fluorescent probe is capable of rapidly responding and highly selectively detecting the palladium ions. The fluorescent probe for detecting the palladium ions has a structure as shown in a formula 1 or 2, wherein R is H, CH3 or t-Bu.
Owner:NANJING UNIV OF TECH
A kind of copper nanocluster fluorescent material and preparation method thereof
ActiveCN107603604BThe preparation method is simple and environmentally friendlySimple and fast operationNanotechnologyLuminescent compositionsPhysical chemistryCopper nanoparticle
Owner:FUJIAN MEDICAL UNIV
Glutathione fluorescence probe as well as preparation method and application thereof
InactiveCN103289681BStrong green fluorescenceHigh detection sensitivityOrganic chemistryFluorescence/phosphorescenceFluoresceinDinitrofluorobenzene
The invention relates to a glutathione fluorescence probe as well as a preparation method and an application thereof. A probe structure is shown in a formula (I). The preparation method comprises the following steps of: dissolving 2,4-dinitrofluorobenzene, fluorescein and potassium carbonate into anhydrous dimethyl formamide (DMF); after reaction, preparing the glutathione fluorescence probe; the application of the glutathione fluorescence probe refers to detection on contents of glutathione having non-diagnostic property. According to the glutathione fluorescence probe as well as the preparation method and the application of the glutathione fluorescence probe, the fluorescence reaction only on the fluorescence probe and glutathione is carried out while the fluorescence reaction on the fluorescence probe and other amino acids is avoided, so that the glutathione fluorescence probe has high selectivity and specificity; the preparation process of the probe is easy and feasible, and the probe is easy for scale production.
Owner:NANJING TECH UNIV
A kind of antigenic epitope peptide of F protein of Peste des petits ruminants virus and its determination, preparation method and application
ActiveCN107033226BStrong green fluorescenceImprove responseSsRNA viruses negative-senseViral antigen ingredientsPeptide antigenDisease
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
A rt-lamp visualization kit for detecting goose Newcastle disease virus and its application
The invention discloses an RT-LAMP visualkit for detecting goose newcastle disease virus (NDV) and application thereof. The visual kit comprises a primer group and color materials, wherein the primer group is a sequence as shown by SEQ ID No: 1 to 4, and the color materials are calcein and manganese chloride. An application method of the kit comprises the following steps of firstly preparing an RT-LAMP reaction system, which comprises AMV reverse transcriptase, 10-time reaction buffer, strand displacement DNA polymerase, dNTP mixture, glycine betaine, the calcein, the manganese chloride, MgSO4, an FIP primer, a BIP primer, an F3 primer, a B3 primer and a sample RNA to be tested; then reacting the reaction system for 50min at the constant temperature of 61 to 65 DEG C, and finally inactivating for 4min at the temperature of 80 DEG C; interpreting a result through natural illumination or ultraviolet light or interpreting through the natural illumination and the ultraviolet light; if a reaction product is green under the natural light, indicating that the sample to be tested contains the NDV; if the reaction product shows obvious green fluorescence under the ultraviolet light, indicating that the sample to be tested contains the goose NDV.
Owner:中科生物技术转移(深圳)有限公司 +1
Targeting peptide modified gold cluster iron oxide assembly material radiotherapy sensitizer
ActiveCN114129726AStrong green fluorescenceHas aggregation-induced luminescent propertiesHeavy metal active ingredientsPharmaceutical non-active ingredientsTumor targetingArginine
The invention discloses a gold cluster iron oxide assembled material radiotherapy sensitizer, and belongs to the crossing field of nanomaterial chemistry and biochemistry. The preparation method comprises the following steps: assembling an Au4 cluster solution with ferric oxide in a cell culture solution, and then modifying the surface of the Au4 cluster solution with cyclic arginine-glycine-aspartic acid-phenylalanine cysteine (cRGD) peptide, so as to obtain Au4-IO NP-cRGD. The gold cluster iron oxide assembled material has strong green fluorescence at room temperature, has good dispersibility and tumor targeting, and can be used for high-quality cell imaging. The gold cluster iron oxide assembly material can be used as a radiotherapy sensitizer to achieve a radiotherapy sensitization effect, the X-ray dosage is reduced, and the high efficiency of killing tumor cells is achieved.
Owner:ZHENGZHOU UNIV
Cultivation method of transgenic zebrafish for detecting organic pollutants in water body
PendingCN114561427AHigh green fluorescence intensityHigh luciferase activityGeneral water supply conservationMicrobiological testing/measurementMosquitofishWater quality
The invention discloses a cultivation method of transgenic zebrafish for detecting organic pollutants in a water body. The transgenic zebrafish is prepared by using a promoter for expressing the mosquito catfish cyp1a gene, plasmids for simultaneously expressing green fluorescent protein and luciferase, and body-color-free zebrafish. In the actual water quality monitoring work, technicians in the field can qualitatively evaluate the pollution condition of organic matters in the water body by directly observing the fluorescence of the transgenic zebra fish, and also can quantitatively analyze pollutants in the water body by measuring the activity of luciferase of the transgenic zebra fish.
Owner:SOUTH CHINA AGRI UNIV
Peptides des petits ruminants virus hn protein antigen epitope peptide h362 and its determination, preparation method and application
ActiveCN107216372BStrong green fluorescenceGood reactogenicitySsRNA viruses negative-senseViral antigen ingredientsHN ProteinF protein
The invention relates to an epitope peptide H362 of an HN protein in PPRV, and determination, a preparation method and application thereof. The amino acid sequence of the epitope peptide is H362: <362>EANWVVPSTDVRDL<375>. The invention detects reactogenicity of a monoclonal antibody and PPRV and specificity of the monoclonal antibody; according to detection results, the monoclonal antibody has good reactogenicity to rPPRV-HN-F protein; immunoinformatic technology is cooperatively used for predicating the B cell epitope of the HN protein; an aminated ELISA plate is employed for detecting candidate epitopes and the monoclonal antibody 10E3, and the epitope peptide H362 corresponding to 10E3 is determined; and determination of the epitope peptide lays a theoretical foundation for preparation of epitope vaccine antigens and diagnostic reagent antigens for PPRV.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Method based on spinach green RNA visualization and application thereof
ActiveCN112280798ARealize visualizationStrong green fluorescenceFluorescence/phosphorescenceVector-based foreign material introductionAptamerSpinacia
The invention discloses a method based on spinach green RNA visualization and application thereof, and belongs to the technical field of biology. The invention comprises the application of plant tRNAin protecting and / or stabilizing the interaction between a spinach green RNA aptamer and a fluorescein molecule DFHBI and realizing the visual application of the spinach green RNA in the plant body. The method comprises the following steps: constructing lysine transport RNA-spinach green RNA-lysine transport RNA, subcloning a product into a pEAQHT vector to obtain pEAQHT / KSK, attacking the pEAQHT / KSK into chloroplast-free onion epidermis cells by using a gene gun method, putting onion epidermis on a hypertonic culture medium, infiltrating the onion epidermis into a DFHBI solution, and observing strong enough green fluorescence under a laser confocal microscope. According to the invention, the visualization of RNA in living cells of plants is realized by utilizing methods such as molecularsignal marking, RNA binding marker protein, the RNA-based aptamer and the like.
Owner:HANGZHOU NORMAL UNIVERSITY
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