32results about How to "Strong green fluorescence" patented technology

The invention discloses a method for preparing a Hirsutella sinensis transforming strain expressing green fluorescent protein. The method comprises: (1) preparing a Hirsutella sinensis blastospore suspension; (2) preparing Agrobacterium tumefaciens containing recombinant plasmid pRF-AETH; (3) preparing an Agrobacterium tumefaciens liquid; (4) mixing the Hirsutella sinensis blastospore suspension and the Agrobacterium tumefaciens liquid, and co-culturing; and (5) carrying out selective culture, identifying, and obtaining the Hirsutella sinensis capable of stably expressing green fluorescent protein. The invention further provides a Hirsutella sinensis transforming strain. According to the present invention, with the method, the Hirsutella sinensis transforming strain capable of stably expressing egfp gene can be obtained, can provide the convenience for the molecular biology research of the Hirsutella sinensis functional gene, can monitor the growth and reproduction and the development and differentiation of the Hirsutella sinensis in host insects, can provide the direct evidence for the clarification of the interaction between the Hirsutella sinensis and the host, and has excellent application prospects.

The invention discloses a gold ion fluorescence probe, a preparation method and an application thereof. The fluorescence probe has high selectivity and high sensitivity, can be used for detecting gold ions. The gold ion fluorescence probe is a 4,5-dihydrazine pyridyl fluorescein derivative shown in a formula I, the preparation method comprises the following steps of reacting 4,5-fluorescein bi-aldehydes and hydrazinopyridines in an ethanol solution to generate the dihydrazine pyridyl fluorescein derivative which can be taken as the gold ion fluorescence probe.

The invention discloses a reverse transcription loop-mediated isothermal amplification (RT-LAMP) visual kit for detecting Japanese B encephalitis virus and application of the kit. The visual kit contains a primer group and color development substances, wherein primers have sequences shown as SEQ ID NO.1-4, and the color development substances are calcein and manganese chloride. A using method of the kit comprises the following steps of: preparing an RT-LAMP system containing AMV reverse transcriptase, a 1 time reaction buffer solution, strand displacement DNA polymerase, a dNTP mixture, betaine, calcein, manganese chloride, MgSO4, a forward inner primer (FIP), a backward inner primer (BIP) group, an F3 primer, a B3 primer and RNA of a sample to be detected; performing thermostatic reaction on the reaction system at the temperature of between 61 and 65 DEG C, and inactivating; and judging a result under natural light or ultraviolet or natural light and ultraviolet, wherein if the reaction product is green under the natural light, the sample to be detected contains the Japanese B encephalitis virus; and if the reaction product represents obvious green fluorescence under the ultraviolet, the sample to be detected contains the Japanese B encephalitis virus.

The invention discloses a copper nano-cluster fluorescent material and a preparation method thereof. Copper nanoparticles protected by ascorbic acid are used as a precursor to synthesize the copper nano-cluster fluorescent material from top to bottom. Ammonia water is used as an etching agent to control the formation of a copper nano-cluster. The preparation method of the novel copper nano-clusterfluorescent material, provided by the invention, has the advantages of simplicity in preparation, environment protection, rapidness, low price and the like. The prepared copper nano-cluster has strong green fluorescent light; the maximum emission wavelength is 498nm and the yield of quanta is 6.63 percent.

The invention relates to peste des petits ruminant virus F protein epitope peptide. The amino acid sequences of the epitope peptide are respectively as follows: F176: <176>DYINNELVPSVHRMSCEL<193>, or / and F347: <347>MSPLLQECFRGSTKS<361>, or / and F391: <391>CKCYTTETVINQDPDKLL<408>, or / and F417: <417>TVINQDPDKGPVGSREYPD<435>, or / and F430: <430>SREYPDSVYLH<440>, or / and F502: <502>VSLGLVTLICCCKGRCRNK<520>. B cell epitopes of a target protein are predicted, the different predicted epitopes are respectively artificially synthesized, the reactogenicity with an antibody of F protein is detected, and therefore, the B cell epitopes of the PPRV F protein are authenticated.

The invention discloses an RT-LAMP visualkit for detecting goose newcastle disease virus (NDV) and application thereof. The visual kit comprises a primer group and color materials, wherein the primer group is a sequence as shown by SEQ ID No: 1 to 4, and the color materials are calcein and manganese chloride. An application method of the kit comprises the following steps of firstly preparing an RT-LAMP reaction system, which comprises AMV reverse transcriptase, 10-time reaction buffer, strand displacement DNA polymerase, dNTP mixture, glycine betaine, the calcein, the manganese chloride, MgSO4, an FIP primer, a BIP primer, an F3 primer, a B3 primer and a sample RNA to be tested; then reacting the reaction system for 50min at the constant temperature of 61 to 65 DEG C, and finally inactivating for 4min at the temperature of 80 DEG C; interpreting a result through natural illumination or ultraviolet light or interpreting through the natural illumination and the ultraviolet light; if a reaction product is green under the natural light, indicating that the sample to be tested contains the NDV; if the reaction product shows obvious green fluorescence under the ultraviolet light, indicating that the sample to be tested contains the goose NDV.