31 results about "Turkey Herpesvirus" patented technology

Promoter genes that are derived from Marek's disease virus (MDV). These promoter genes can express two foreign genes when inserted in a recombinant turkey herpesvirus (HVT). A recombinant HVT having said novel promoter gene between two foreign genes. The poultry vaccine consisting of the recombinant turkey herpesvirus described in the present invention.

The present invention provides a recombinant turkey herpesvirus modified by the presence of the cDNA encoding the hemagglutinin protein of avian influenza virus under a promoter. A poultry vaccine comprising the recombinant turkey herpesvirus described in the present invention can induce serological responses that may be easily detected by the hemagglutination inhibition assay but not by commercially available diagnostic ELISA kits; thus enabling easy differentiation between vaccination and field infection.

The invention discloses a recombinant turkey herpes virus expressing H9N2 subtype avian influenza virus H9 protein. The recombinant turkey herpes virus is obtained by carrying out the following transformation on the genome of the turkey herpes virus: the DNA molecule containing the coding gene of the H9N2 subtype avian influenza virus H9 protein is added; the amino acid of the H9N2 subtype avian influenza virus H9 protein The sequence is shown as sequence 2 in the sequence listing. The experiment proved that on the first day of the experiment, the recombinant turkey herpes virus was subcutaneously inoculated into the neck of 1-day-old SPF chickens; on the 35th day of the experiment, the BJ / 15 virus strain was inoculated into the nasal cavity; Neither the cloaca nor the cloaca are detoxified, and the BJ / 15 virus strain cannot efficiently replicate in the organs (such as internal organs, trachea, lung, spleen and kidney) of SPF chickens. It can be seen that the recombinant turkey herpes virus has excellent immune protection effect on H9N2 subtype AIV. The invention has important application value.

The invention discloses construction and application of a recombinant turkey herpesvirus expressing H7N9 subtype highly pathogenic avian influenza virus HA protein. The recombinant turkey herpesvirusis obtained by inserting an exogenous gene expression box between the HVT-065 and HVT-066 genes in a non-essential replication area of a coding sequence of a turkey herpesvirus (HVT), wherein the exogenous gene expression box is formed by connecting an MCMV promoter, an exogenous gene and SV40 poly A in sequence; the exogenous gene is the H7HA gene, and the nucleotide sequence of the exogenous gene is shown as SEQ ID NO:1. The construction method of the recombinant turkey herpesvirus is also provided. An ultrasonic cracking and breaking method is adopted for purification, the screening time isshortened, and the obtained recombinant turkey herpesvirus rHVT-H7HA provides good protection for the highly pathogenic H7N9 subtype avian influenza virus and can be used for subsequent development of new avian influenza virus vaccines.

The invention discloses a method for measuring viral titer of a Marek's disease (MD) turkey herpesvirus vaccine, live. The method comprises the following steps of: (1) preparing chick embryo fibroblast suspension; (2) performing cell culture; (3) diluting a virus solution to be measured; (4) performing virus inoculation and culture; (5) counting plaques; and (6) calculating the viral titer. The method is easy to impellent, uniform in measurement conditions, strong in controllability and small in batch errors, and shortens the period of measuring the viral titer of the MD turkey herpesvirus vaccine, live.

The invention relates to construction of a recombinant turkey herpesvirus used for expressing an F gene of a chicken Newcastle disease virus and an application of the construction. The recombinant turkey herpesvirus used for expressing the F gene of the chicken Newcastle disease virus is named as recombinant turkey herpesvirus rHVT-F strain; the virus strain combines an expression cassette containing the F gene of the chicken Newcastle disease virus with the turkey herpesvirus (HVT), that is, and the F gene controlled by a CMV promoter is inserted into an HVT gene to construct to obtain the brand new promoter rHVT-F strain. The rHVT-F virus strain can induce a chicken to produce lasting protective immunity on MD and ND resistance. The virus strain produces a strain to prepare a bivalent recombinant vaccine for the fowl paralysis and the chicken Newcastle disease.

The present disclosure provides a recombinant viral vector comprising at least one transgene inserted into a Marek's disease viral genome for treatment of diseases in poultry. Also provided are immunogenic compositions comprising such recombinant viral vectors and methods for preventing or inhibiting Marek's disease in combination with at least a second disease in poultry.