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442 results about "Avian influenzavirus" patented technology
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H5 subtype avian flu virus hemagglutinin protein monoclonal antibody, and its preparing method and use
This invention relates to a monoclonal antibody capable of combining with H5 subtype avian influenza virus HA protein specifically, the hybridoma cell line secreting said antibody and a preparing method. The invention also relates to a serial test kit for testing H5 subtype avian influenza virus by the antibody and a bit of said antibody in the test sample of the virus and its usage in treatment.
Owner:XIAMEN UNIV
GeXP (Gene Expression Profiler) detection kit for differentiating 11 kinds of duck viral diseases
ActiveCN103773899AStrong specificityImprove throughputMicrobiological testing/measurementDNA/RNA fragmentationDiseaseDuck hepatitis A virus
Owner:GUANGXI VETERINARY RES INST
Primer system and method for detecting and analyzing avian influenza virus
InactiveCN101058833AQuick checkAccurate detectionMicrobiological testing/measurementDNA preparationFowlHypotype
The invention provides a method for detecting fowl influenza virus and parting primer system and augmenting fowl influenza virus RNA asymmetrically. The invention designs and screens 25 pairs multiple asymmetrical RT-PCR primers, a pair general primer, 52 specific probes and 3 quality control probes according to the report fowl influenza virus total hypotype genome sequence in Genebank, wherein every pair in 25 pairs multiple asymmetrical RT-PCR primers is fit for a hypotype of fowl influenza virus, the primer system comprising 25 pairs multiple asymmetrical RT-PCR primers and a pair general primer can be used for detecting and parting fowl influenza virus, the primer system builds the method of asymmetrically augmenting fowl influenza virus RNA and detecting and parting fowl influenza virus. The invention provides strong specificity, high sensibility, which also proceeds the first step application.
Owner:中国检验检疫科学研究院动植物检疫研究所
Method of inhibiting the transmission of influenza virus
ActiveUS20070280901A1Rapid and antiviral effectivenessHigh activityCosmetic preparationsBiocideOrganic acidAlcohol
Antimicrobial compositions having a rapid and persistent antiviral effectiveness against influenza viruses, including avian flu viruses, are disclosed. The antimicrobial compositions contain (a) a disinfecting alcohol, (b) an organic acid, and (c) water, wherein the composition has a pH of about 5 or less and the nonvolatile components of the composition are capable of forming a barrier film or layer on a treated surface.
Owner:HENKEL KGAA
Gene encoding hemagglutinin protein of H5 avian influenza virus and its application
ActiveCN1632124AHigh level of immune responseImproving immunogenicityViral antigen ingredientsAntibody ingredientsHemagglutininHighly pathogenic
The present invention relates to an artificially synthesized gene optiHA containing codons for chicken partial tropism. Its reading frame contains 1707 bp nucleotides and encodes a total of 568 amino acids. The gene is compatible with H5 subtype highly pathogenic avian influenza virus A / Goose / GuangDong / 1 / 96(H5N1)[GD / 1 / 96(H5N1)]hemagglutinin (HA) gene has a nucleotide homology rate of 70%, an amino acid homology rate of 100%, and encodes the H5 subtype Hemagglutinin (HA) protein of avian influenza virus GD / 1 / 96 (H5N1). The invention also relates to the application of the gene as an immunogenic gene of H5 subtype influenza DNA vaccine and other genetic engineering vaccines.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Targets for human micro rnas in avian influenza virus (H5N1) genome
The present invention relates to targets for Human microRNAs in Avian Influenza Virus (H5N1) Genome and provides specific miRNA targets against H5N1 virus. Existing therapies for Avian flu are of limited use primarily due to genetic re-assortment of the viral genome, generating novel proteins, and thus escaping immune response. In animal models, baculovirus-derived recombinant H5 vaccines were immunogenic and protective, but results in humans were disappointing even when using high doses. Currently, two classes of drugs are available with antiviral activity against influenza viruses: inhibitors of the M2 ion channel, amantadine and rimantadine, and inhibitors of neuraminidase, oseltamivir, and zanamivir. There is paucity of information regarding effectiveness of these drugs in H5N1 infection. These drugs are also well known to have side effects like neurotoxicity. Thus there exists a need to develop alternate therapy for targeting the Avian flu virus (H5N1). The present invention addresses this need in the field.
Owner:COUNCIL OF SCI & IND RES
Preparation method of avian influenza virus growing in serum-free full-suspended cultured MDCK cells and obtained avian influenza virus
ActiveCN106011083AStable characteristicsGet rid of dependenceVertebrate cellsArtificial cell constructsSerum freeF1 generation
The invention discloses a preparation method of an avian influenza virus suitable for growing in a serum-free full-suspended cultured MDCK cell line and the avian influenza virus obtained through the method. The preparation method comprises the following steps of 1 preparation of the MDCK cells to be inoculated; 2 virus seed preparation, wherein a chick embryo source avian influenza virus is prepared; 3 F1 generation virus domestication; 4 F2 generation virus domestication, wherein a supernatant sample retained at the time point when the blood clotting titer of the avian influenza virus obtained in the F1 generation is highest is taken, and the step 3 is repeated; 5 F3 generation virus domestication, wherein the F3 generation virus culturing temperature is 35 DEG C; 6 F4 generation-F10 generation virus domestication, wherein the step 5 is repeated, and the domesticated avian influenza virus is obtained. According to the preparation method, through a domestication method, the avian influenza virus is directly domesticated to completely adapt to be efficiently reproduced on the serum-free full-suspended cultured MDCK cells from the mode of being cultured by a chick embryo, the domestication efficiency is high, the avian influenza virus can be efficiently infected and copied in the MDCK cells, and the virus characteristic is stable.
Owner:ZHAOQING DAHUANONG BIOLOGIC PHARMA +2
Human anti-H7N9 avian influenza virus neutralizing antibody 1F7L and its use
InactiveCN107226861AEffective neutralizationAvoid infectionImmunoglobulins against virusesAntiviralsNatural Killer Cell Inhibitory ReceptorsNeutralizing antibody
The invention discloses a human anti-H7N9 avian influenza virus neutralizing antibody 1F7L screened by a single cell sorting technology. The amino acid sequences in the light and heavy chain variable regions are shown in the formulas of SEQ ID No. 2 and SEQ ID No. 5. The antibody has the ability to neutralize the H7N9 influenza viruses in vitro and mediates the killing (ADCC) of the H7N9 influenza virus-infected cells with effector cells mainly comprising NK cells. The antibody can be used as a treatment drug for highly pathogenic avian influenza infection and can also be used for the development of H7N9 influenza virus antigen detection reagents.
Owner:THE THIRD PEOPLES HOSPITAL OF SHENZHEN
Recombinant flu vaccines
InactiveUS20090117144A1Sufficient immunogenicityReduce loadSsRNA viruses negative-senseSsRNA viruses positive-senseInfluenza virus vaccinePlant virus
The present invention provides compositions for use as vaccines against the influenza virus, and rapid methods of producing such compositions. The composition include i) at least one peptide derived from an influenza virus, wherein the peptide is fused to a capsid protein derived from a plant virus forming a recombinant capsid fusion peptide and ii) at least one isolated antigenic protein or protein fragment derived from a human or avian influenza virus. The isolated antigenic protein or protein fragment derived from the human or avian influenza virus can be conjugated to the surface of the recombinant capsid fusion peptide.
Owner:PFENEX
Colloidal gold immunity percolation sensitization method for detecting avian influenza virus and its reagent kit
The invention provides a colloidal gold immunity infiltration sensitivity raising method for checking avian influenza virus and a reagent kit therefore. The colloidal gold immuno infiltration sensitivity raising method comprises: (1) preparing an immunity infiltration test strip; (2) fixing influenza virus multi-clonal antibody on the immunity infiltration test strip; (3) preparing avian influenza virus gold marking probes; 4) preparing sensitivity raising agent; (5) detecting avian influenza virus. The invention utilizes the oxidation reduction reaction between chloric acid and ac=scorbic acid, to generate gold atoms which can be absorbed by colloidal gold, to position the deposition part of the colloidal gold to develop and enhance color, thereby improving the detection sensitivity on object antigen by 8 to 50 times. Based on colloidal gold spot immunity infiltration test method (double-antibody sandwich), the invention adopts nanometer gold particle sensitivity raising technique, to improve the detection sensivity on avian influenza virus, screen sample of high flux, and adapt avian influenza virus detection in basic layer or in-situ field.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE
Waterless Peramivir crystal and medicament composition thereof
ActiveCN101314579AGood reproducibilityKeep dryOrganic active ingredientsOrganic chemistryMedicineAvian influenza virus
The invention relates to an anhydrous peramivir crystal compound and a preparation method thereof, and further discloses a pharmaceutical composition comprising the same and the application thereof in the preparation of medicaments for treating the influenza virus and the Avian influenza virus.
Owner:天津泰普制药有限公司
Flu/human avian influenza virus detection gene chip and production method and use
InactiveCN101392302AShort detection timeImprove detection accuracyMicrobiological testing/measurementHighly pathogenicTyping
The invention discloses an influenza / human and avian influenza virus detection gene chip and a preparation method and an application thereof, 102 probes used for detecting A type, B type, H1N1 hipotype, H3N2 hipotype, H5N1 hipotype and H9N2 hipotype influenza viruses and 4 highly pathogenic differential diagnosis probes, as well as a process of sample treatment, hybridization, elution and chip identification which can carry out detection, typing or identification over the 6 types of viruses simultaneously and can identify the high pathogenicity of viruses. The invention not only greatly shortens the detection time of influenza viruses, but also improves the detection accuracy and provides a feasible technique support for the early warning mechanism of influenza epidemic situation in fields of clinical diagnosis, inspection quarantine, and the like.
Owner:中国疾病预防控制中心病毒病预防控制所 +1
H9N2 avian influenza virus vaccine strain and application of H9N2 avian influenza virus vaccine strain in immune protection
The present invention relates to the field of animal virology, and provides a recombinant chicken-origin H9N2 avian influenza virus vaccine strain and a method for isolation, identification and purification of the strain. The invention further relates to a research of biological characteristics of the strain, especially to a research of characteristics of the strain adopted as the vaccine strain,and an evaluation of immune effects of the strain on SPF chickens. The preservation number of the strain is CCTCCNO:V201030. According to the present invention, the antigen variation conditions of the virus strain and other isolated virus strains are represented from the molecular level; after the virus strain is prepared into the vaccine, the prepared vaccine is adopted to immunize the 4 week old SPF chickens, with the protection effect analysis of the homologous H9 influenza wild virus strain and the heterologous H9 influenza wild virus strain, the results show that the influenza virus strain can be adopted as the spare vaccine strain of H9 subtype avian influenza. With the present invention, the spare vaccine strain is provided for prevention of the avian influenza outbreak by using the vaccine, the molecular biology technology program is provided for screen of the avian influenza virus vaccine strain, the molecular biology background is provided for study of the mechanism of animal infection by the avian influenza, and the important public health significance is provided.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
Turkey herpesvirus vectored recombinant containing avian influenza genes
InactiveUS20080241188A1Easy to distinguishEasy to detectSsRNA viruses negative-senseVectorsVaccinationElisa kit
The present invention provides a recombinant turkey herpesvirus modified by the presence of the cDNA encoding the hemagglutinin protein of avian influenza virus under a promoter. A poultry vaccine comprising the recombinant turkey herpesvirus described in the present invention can induce serological responses that may be easily detected by the hemagglutination inhibition assay but not by commercially available diagnostic ELISA kits; thus enabling easy differentiation between vaccination and field infection.
Owner:ZEON CORP +1
Fluorescent quantitative PCR primer, probe, kit and detection method for detecting avian influenza subtype
ActiveCN106435024AHigh detection sensitivityHigh sensitivityMicrobiological testing/measurementAvian influenza virusRepeatability
The invention provides a multiplex fluorescent quantitative PCR primer and a probe for detecting H5, H7 and H9 subtype avian influenza viruses. With adoption of the primer and the probe, multiplex fluorescent quantitative PCR can be adopted to simultaneously detect which subtypes the H5, H7 and H9 subtype avian influenza viruses concretely are, no influences exist among the primers of different subtypes, the specificity is strong, the detection sensitivity is 10-50 copy numbers, a target sequence can be accurately detected quantitatively and qualitatively, the repeatability is good, and the reliability is high. The invention provides a multiplex fluorescent quantitative PCR kit for simultaneously detecting the H5, H7 and H9 subtype avian influenza viruses. The kit has the detection sensitivity being 10-50 copy numbers and is high in sensitivity and strong in specificity. The invention also provides a detection method which is good in stability. With adoption of plasmids with gene fusion as a positive standard substance, tedious operation of changing the positive standard substance for multiple times is avoided, the detection time is greatly shortened, the detection times are greatly reduced, and detection of a sample can be finished within 2h.
Owner:NANJING AGRICULTURAL UNIVERSITY
Gene engineering antibody of human source neutrality for anti virus H5N1 of bird flu
This invention provides a human-derived genetic engineering antibody that can specifically bond avian influenza virus H5N1. The genetic engineering antibody is derived from antibody gene library of avian influenza virus H5N1 patients in convalescence. The recombinant antibody is decided by hypervariable region (CDRs) specific gene sequence in the antibody light chain and heavy chain gene variable region, and is effectively expressed in procaryotic cells and eukaryotic cells. The recombinant antibody is a functional neutralizing antibody can specifically bond avian influenza virus haemagglutinin protein HA. Part or all genes of the antibody in CDR can modify and produce genetic engineering antibodies with different forms in procaryotic cells, yeast, insects and eukaryotic cells, and can prevent and treat diseases related to avian influenza virus H5N1.
Owner:中国疾病预防控制中心病毒病预防控制所
Immunogenic peptides of influenza virus
Peptides and polypeptides that elicit immunogenic responses in a mammal; especially neutralizing antibodies, against human and avian influenza strains H1N1, H3N2, H5N1 and H7N7 are disclosed Immunogenic compositions including these peptides, and polypeptides are also provided. Compositions including these peptides and polypeptides with or without adjuvants are disclosed. Nucleic acids and expression cassettes encoding these peptides and polypeptides are also disclosed. Methods of inhibiting infection by influenza, with or without cell entry, are also disclosed using these peptides and polypeptides.
Owner:UNITED STATES OF AMERICA
Humanized avian influenza virus H7N9 resisting neutralizing antibody M5 as well as preparation method and application thereof
ActiveCN104031146AImmunoglobulins against virusesAntiviralsAcute respiratory tract infectionNeutralizing antibody
The invention provides a humanized avian influenza virus H7N9 resisting neutralizing antibody M5 which is obtained through screening by using a phage surface display technology. Amino acid sequences of light-chain and heavy-chain variable regions of the antibody are respectively represented by SEQ ID No. 1 and SEQ ID No. 2. The antibody can specifically identify a particulate antigen of a virus H7N9, can be subjected to remarkable enzyme-linked immunoreaction with the virus H7N9 and has the neutralizing active function of resisting virus H7N9 infection; in addition, the antibody provided by the invention can be prepared into specific antibody drugs for preventing and treating the avian influenza virus H7N9 and sequentially is clinically used for preventing and treating acute respiratory tract infections caused by the virus H7N9.
Owner:INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
Detection kit and detection method for H1, H3 and H9 type avian influenza viruses
ActiveCN105296670AEasy to detectShorten detection timeMicrobiological testing/measurementForward primerRNA extraction
The invention discloses a detection kit and detection method for H1, H3 and H9 type avian influenza viruses and belongs to the technical field of biology. The kit comprises an RNA extraction kit, an RNA reverse transcription kit and an RPA detection kit. The RNA extraction kit is used for extracting RNA of a to-be-detected sample to be used as a detection template. The RNA reverse transcription kit is used for conducting reverse transcription on the RNA in the detection template to obtain cDNA. The RPA detection kit comprises primers and fluorescent dye. The primers are used for amplifying the cDNA to obtain an amplified DNA sequence. The primers comprise the forward primer and the reverse primer. The fluorescent dye is used for conducting fluorescence detection on the amplified DNA sequence. According to the detection kit and the detection method, the avian influenza viruses can be rapidly, easily and conveniently detected.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY SCI SHANXI ACAD OF AGRI SCI SAAS +1
Recombined chicken alpha interferon gene and recombinant vector thereof
InactiveCN101338314AStrong antiviral activityEase of mass productionFungiMicroorganism based processesHigh concentrationAnti virus
The invention relates to a novel gene sequence of a recombinant chicken Alpha interferon, constructs a recombinant expression vector thereof and belongs to a gene engineering biological product obtained by a molecular biology method. The gene sequence of a newly designed chicken Alpha interferon is recombined into a pPICZ Alpha-A vector and then is confirmed on the special position of a microzyme by an electric conversion mode. Besides, a pichia expression system is adopted to express a foreign gene, thus being beneficial to the commercial production of the chicken interferon. The protein expressed by a gene group after being diluted by 4365158.3 times can completely restrain the attraction of vesicular stomatitis virus of 100-1000TCID50. Compared with a natural chicken Alpha interferon, the novel gene sequence of a recombinant chicken Alpha interferon has a higher anti-virus effect; the anti-virus effect thereof is improved by 8 times. Test also detects that the protein expressed by the gene can restrain the proliferation of a newcastle disease virus and an avian influenza virus; besides, the effect of the interferon with high concentration is more remarkable.
Owner:NANJING AGRICULTURAL UNIVERSITY
Gene chip for detection and typing of bird flu virus
The present invention relates to gene chip for the detection and subtyping of bird flu virus. The present invention establishes gene chip with high specificity, high sensitivity and great practicability for detection and subtyping of bird flu virus through designing and screening 50 pairs of specific primers, 2 universal primers, 52 specific probes and 3 quality control probes for the multiple RT-PCR subtyping detection of bird flu virus genes based on the gene sequences of different bird flu virus subtypes Genebank reports; and performing the effectiveness test, specificity test, sensitivity test and condition optimization of gene chip detection. The present invention establishes also the detection and subtyping process with the chip and gives the initial application.
Owner:中国检验检疫科学研究院动植物检疫研究所
Triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus
ActiveCN103342749ADecreased levels of maternal antibodiesInvulnerableEgg immunoglobulinsImmunoglobulins against virusesInfectious bronchitisInfectious laryngotracheitis virus
The invention discloses a triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus and a preparation method and application thereof. The preparation method of the egg yolk antibody comprises the following steps of: (1) selecting a hen; (2) performing triple inactivation immunization of the hen obtained by the step (1) by use of newcastle disease virus, infectious bronchitis virus and avian influenza virus; (3) taking the egg laid by the hen and separating the egg yolk liquid; and (4) separating and purifying an egg yolk antibody from the egg yolk liquid obtained by the step (3). The triple egg yolk antibody disclosed by the invention is of great value in treating and preventing newcastle disease, avian influenza and infectious bronchitis.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Recombined newcastle disease virus LaSota attenuated vaccine strain for expressing avian influenza virus H9 subtype HA protein
The invention relates to a recombinant newcastle pestilence LaSota HCLV expressing the bird flu virus H9 subtype hemagglutinin (HA) protein, in particular the recombinant newcastle pestilence LaSota is rL-H9HA. The invention further discloses a method for preparing the recombinant newcastle pestilence LaSota HCLV and the application of the HCLV in preparing a bivalent vaccine for preventing bird flu and newcastle pestilence.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Method of inhibiting the transmission of influenza virus
Owner:HENKEL KGAA
Antigen-antibody complex for preventing and/or treating avian influenza
ActiveCN101732716AChange submission pathEnhance immune functionAntiviralsAntibody ingredientsAvian influenza virusOrganism
The invention provides an antigen-antibody complex for preventing and / or treating avian influenza, which comprises inactivation avian influenza totiviruses which are used as antigen and an immune body thereof, and the mass concentration ratio of the antigen and the immune body is more than 1. After entering organisms to perform initial immunization, the antigen-antibody complex stimulates the organisms again to induce immunoreaction, and immune response is quick in speed and strong in reaction; the antigen-antibody complex used as a carrier is more favorable for capturing and presenting antigen presenting cells and can also strengthen a breeder reaction of T cells effectively; purified totiviruses used as the antigen increase the molecular weight of the complex, are more favorable for ingestion of immunocyte of the organisms on the antigen, cause the more extensive immunoreaction quickly, and have a significance for preventing avian influenza viruses of which the antigen is easy for variation. A preparation of the antigen-antibody complex does not need to use solid carriers, does not cause intense stimulus on the organisms or initiates the organisms to generate an adverse reaction, and is simple to prepare and safe and convenient to use.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
Isothermal amplication rapid detection method of H7N9 avian influenza virus
InactiveCN103305634AMicrobiological testing/measurementDNA/RNA fragmentationDirect observationAvian virus
The invention discloses a method for detecting H7N9 subtype avian influenza virus through loop-mediated isothermal amplification (LAMP). The method is used for detecting avian influenza virus in the fields of foods and raw materials, environmental samples, medical samples and health and disease prevention. According to the technical scheme, the method is characterized in that a specific primer is designed with regional gene sequences respectively coded as HA and NA in an avian influenza genome serving as target sequences, and a reaction system is optimized, so as to perform target gene specific amplification. The method only needs a constant-temperature device, does not need the thermal denaturation and long-term temperature cycle of a template, realizes the direct observation of the results, and has the characteristics of being low in cost, high in efficiency and simple in operation. The LAMP detection method of the H7N9 subtype avian influenza virus nucleic acid, created by the invention, has the characteristics of being high in specificity, high in sensitivity and convenient and quick, can be carried out in a grass-roots level or a small test site, and brings a novel technology and method for detecting the H7N9 subtype avian influenza virus.
Owner:ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Virus detector
ActiveCN104165906AThe detection process is fastOperation semi-automaticMaterial impedanceBird fluMicroelectrode
The invention discloses a virus detector, which comprises an interdigital array microelectrode (1), a micro fluidic detection pool (2) and an impedance detection module (3); when a sample containing virus is injected into the micro fluidic detection pool (2), an antibody or other biological identification materials fixed on the surface of the interdigital array microelectrode (1) embedded in the micro fluidic detection pool (2) can be combined with virus, impedance change is generated, impedance measure is carried out through an impedance detection module (3), data processing is carried out by using a standard curve, so that bird flu virus content can be rapidly and quantitatively determined. The virus detector has the advantages of fast detection speed, convenient operation, quantitative analysis and on-site detection.
Owner:CHINA AGRI UNIV
Method for silencing IFNARI gene in DF-1 cell line
ActiveCN104694576AReduce manufacturing costSolve the insufficient amount of antigenFermentationPlant genotype modificationGene expressionTiter
The invention discloses a method for silencing an IFNARI gene in a DF-1 cell line. The method includes the steps that according to the IFNARI gene, multiple pieces of siRNA of an RNA interference target spot sequence are designed, and the siRNA knocking down IFNARI gene expression at the mRNA level is screened out; a lentiviral vector containing an IFNARI RNAi gene is built; by means of the lentiviral vector, the IFNARI RNAi gene is mediated into the DF-1 cell line to silence IFNARI gene expression, and the proliferation titer of an avian influenza virus in the DF-1 cell line is improved. The method can increase the antigen amount of the avian influenza virus in the DF-1 cell line by 5-10 times, the production cost of a vaccine is greatly reduced, the problems that the vaccine is insufficient in antigen amount and high in production cost can be solved, and the research and development process of an avian influenza bioreactor cell culture technology is promoted.
Owner:POULTRY INST SHANDONG ACADEMY OF AGRI SCI
Surface enhanced Raman spectra substrate for detecting avian influenza virus, and application thereof
ActiveCN104406954AAchieve enrichmentQuick checkRaman scatteringGold particlesSurface-enhanced Raman spectroscopy
The invention discloses a surface enhanced Raman spectra substrate for detecting an avian influenza virus and application of the surface enhanced Raman spectra substrate. The application provided by the invention specifically relates to the application of nano-gold particles coupled with alpha (2 and 3) sialyloligosaccharide in the surface enhanced Raman spectra substrate for detecting the avian influenza virus. Experiments show that the substrate can specifically capture the avian influenza virus in a sample to be detected; the enrichment of avian influenza viruses in the sample can be realized through centrifugal collection, and then the enriched avian influenza viruses are used for SERS (Surface Enhanced Raman Scattering) spectra detection. An interference wave crest caused by sample impurities is effectively removed because of the enrichment of the viruses, and a spectral signal can also be effectively amplified by the nano-gold particles, so that the substrate can be used for the SERS spectra detection of the avian influenza virus. The substrate has a great significance to the rapid detection and identification of the avian influenza virus.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
Triple rapid detection kit of three avian respiratory diseases, and application thereof
ActiveCN104007261AStrong specificityHigh sensitivityBiological material analysisImmunoglobulins against virusesInfectious DisorderRespiratory tract disease
The invention belongs to the field of infectious disease detection of animals, and concretely relates to a triple rapid detection kit of three avian respiratory diseases, and an application thereof. The kit comprises a test paper strip, a test card shell and a sample dilution, and the test paper strip is formed by sequentially pasting an absorbing pad, a cellulose nitrate membrane, a colloidal-gold pad and a sample pad on a non-absorbent support slice; a combination pad is coated with an anti-avian influenza virus monoclonal antibody-colloidal gold marker, an anti-Newcastle disease virus monoclonal antibody-colloidal gold marker and an anti-chicken infectious bronchitis virus monoclonal antibody-colloidal gold marker mixture, detection lines T1, T2 and T3 are coated with the anti-avian influenza monoclonal antibody, the anti-Newcastle disease virus monoclonal antibody and the chicken infectious bronchitis monoclonal antibody, and the cellulose nitrate membrane is coated with a rabbit anti-rat IgG quality control line. The kit can simultaneously complete the disease cause detection of bird flu H5 type, H9 subtype, Newcastle diseases and chicken infectious bronchitis, and has strong specificity and high sensitivity.
Owner:HUAZHONG AGRI UNIV +1
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