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Triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus

A technology for chicken Newcastle disease virus and avian influenza virus, which is applied in the direction of antibodies, antiviral agents, antiviral immunoglobulins, etc., and can solve the problems of drug resistance and drug residues

Active Publication Date: 2013-10-09
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotics can lead to drug residues and drug resistance in animals
The active ingredient of existing vaccines is inactivated virus. Poultry farms usually immunize chickens when they are 1-2 weeks old. It takes about 14 days for the antibodies to be produced in the animal body and play a role. Therefore, when the chickens are 1-4 weeks old During this period, outbreaks of one or more of the above-mentioned diseases are more likely to occur

Method used

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  • Triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus
  • Triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the preparation of liquid egg yolk

[0036] Chicken interferon α (chicken genetically engineered interferon): purchased from Beijing Careinuo Biotechnology Co., Ltd.; it is the protein shown in sequence 1 of the sequence listing; each package is 6ml (containing 30000U chicken interferon α), before use Dilute to 200ml with normal saline and inject 0.1ml per chicken.

[0037] 1. Preparation of egg yolk liquid

[0038] 1. Take a 7-day-old hen and inject chicken interferon-α subcutaneously in the neck, 15U per chicken.

[0039] 2. Select from the hens in step 1 according to the following principles: without artificial immunization; laying has started and laying eggs is normal; after testing, they are negative for Newcastle disease virus, avian influenza virus and chicken infectious bronchitis virus.

[0040] 3. The hen selected in step 2 is immunized as follows:

[0041] Basic immunization: subcutaneous injection of triple inactivated vaccine in the neck, 1...

Embodiment 2

[0062] Embodiment 2, the preparation of egg yolk antibody

[0063] 1. Get the egg yolk liquid prepared by step 1 of embodiment 1 and measure the HI titer of three kinds of viruses as more than 1024 by the method of step 3 of embodiment 1, mix with equal volume of distilled water, shake at 650rpm for 10min, then in In a sealed state, stand at 65°C for 10 minutes, quickly cool to 10°C, then mix with distilled water at a volume ratio of 1:4, adjust the pH to 5.2 with 1M HCl aqueous solution, let stand at 4°C for 6 hours, and then centrifuge at 5000rpm for 20 minutes. Take the supernatant.

[0064] 2. Slowly add an equal volume of saturated ammonium sulfate aqueous solution to the supernatant obtained in step 1 (stir while adding), then let stand at 4°C for 12 hours, centrifuge at 8000rpm for 20 minutes, and take the precipitate.

[0065] 3. Dissolve 1 mass part of the precipitate obtained in step 2 with 3 mass parts of distilled water, then add n-octylic acid to make the volume ...

Embodiment 3

[0071] Embodiment 3, the application of egg yolk antibody

[0072] 1. Application of egg yolk antibody

[0073] Take the 10-day-old sick chickens in the chicken farm, 20 of them have been confirmed to have Newcastle disease (the first group), 20 have been confirmed to have avian influenza (the second group), and 20 have been confirmed to have chicken infectious bronchitis ( The third group), 20 chickens have been confirmed to have Newcastle disease, avian influenza and chicken infectious bronchitis at the same time (the fourth group). The egg yolk antibody prepared in Example 2 was subcutaneously injected into the neck, and each chicken was injected with 1.5 ml. Five days after the egg yolk antibody injection, the cure rate of the first group was 95%, that of the second group was 95%, that of the third group was 100%, and that of the fourth group was 95%.

[0074] 2. Application of egg yolk antibody

[0075] It is applied in multiple chicken farms in Beijing. If a chicken i...

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Abstract

The invention discloses a triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus and a preparation method and application thereof. The preparation method of the egg yolk antibody comprises the following steps of: (1) selecting a hen; (2) performing triple inactivation immunization of the hen obtained by the step (1) by use of newcastle disease virus, infectious bronchitis virus and avian influenza virus; (3) taking the egg laid by the hen and separating the egg yolk liquid; and (4) separating and purifying an egg yolk antibody from the egg yolk liquid obtained by the step (3). The triple egg yolk antibody disclosed by the invention is of great value in treating and preventing newcastle disease, avian influenza and infectious bronchitis.

Description

technical field [0001] The invention relates to a triple yolk antibody against chicken Newcastle disease virus, avian influenza virus and chicken infectious bronchitis virus, its preparation method and application. Background technique [0002] Chicken Newcastle disease is a highly contagious infectious disease caused by Newcastle disease virus, also known as Asian chicken plague or pseudo chicken plague. Its main features are dyspnea, loose stools, nervous disorders, mucous membrane and serosa hemorrhage, high mortality rate, and serious damage to the chicken industry. [0003] Avian influenza is an infectious disease caused by avian influenza virus. It is classified as a Class A infectious disease by the International Office of Epizootics, also known as true chicken plague or European chicken plague. Common symptoms of avian influenza: depression, reduced feed consumption, and emaciation in sick chickens; increased nest performance and decreased egg production in hens; mil...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P31/14A61P31/16
Inventor 刘文军李晶杨利敏李云龙伏显华
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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