44results about How to "Broad cracking spectrum" patented technology

The invention discloses a strong lysis bacteriophage for splitting NDM-1 positive Klebsiella pneumoniae clinical isolates, and an application of the strong lysis bacteriophage used as a biological preparation in the prevention and treatment of NDM-1 Klebsiella pneumoniae induced infection and pollution. The preservation number of the bacteriophage is CCTCC M 2015760, and the bacteriophage has a regularly icosahedral head and a telescopic tail, and belongs to a Myoviridae double-stranded DNA bacteriophage. The bacteriophage has strong kilign activity and wide bacteriophage lysis spectrum, can be used for preparing medicines for preventing and treating NDM-1 Klebsiella pneumoniae induced bacterium infection, and also can be used for killing NDM-1 positive Klebsiella pneumoniae in culture environment and medical environment.

The invention provides a staphylococcus aureus phage strain VB_SavM_JYL01. The staphylococcus aureus phage stain VB_SavM_JYL01 has been preserved in the China Center for Type Culture Collection on 25th March, 2018, the preservation name is Staphylococcus aureus phage VB_SavM_JYL01, and the preservation number is CCTCC M 2018154. The staphylococcus aureus phage strain has a wide lysis spectrum, thus staphylococcus aureus can be lysed, certain staphylococcus epidermidis can also be lysed, host bacteria 206 can be lysed, and staphylococcus clinical strains separated from the environment or fecesof diseased animals can also be lysed; the staphylococcus aureus phage can be used independently or in combination with other substances, and a safe and nontoxic phage disinfecting and killing productis provided for disinfecting and purifying the environment.

The invention relates to the technical field of microorganisms, and particularly relates to a Staphylococcus aureus phage and bacteriostatic application thereof. The Staphylococcus aureus phages107 is sent to China Center for Type Culture Collection for Preservation, the classification name is Staphylococcus aureus phages107, the preservation number is CCTCC NO: M2021125, the preservation date is January 21, 2021, and the address is Wuhan University, Wuhan City, China. The phages107 has high titer up to 2.31 * 10<10>PFU / mL, good pH stability, good temperature stability and wide lysis spectrum, and has lysis ability on 9 strains of Staphylococcus aureus, wherein the 9 strains of Staphylococcus aureus have certain drug resistance and no lysis ability on escherichia coli. The Staphylococcus aureus phage has specific lysis spectrum for Staphylococcus aureus, and has strong lysis ability. The Staphylococcus aureus phages107 can be used for preparation of drugs for treating Staphylococcus aureus induced dairy cow mastitis and other diseases.

The invention discloses a coliphage B_EcoM_C1 with the preservation number of CCTCC NO:M 2017190. The coliphage B_EcoM_C1 is a double-stranded DNA, belongs to a myovirus family, having a regular dodecahedron-shaped head and a telescopic tail, and can form a transparent and bright plaque on a solid medium, no aureoles exist surrounding the plaque, the plaque has clear and regular edge, and the diameter is 0.5-1 mm. The coliphage B_EcoM_C1 has a good eliminating and killing effect on enterohemorrhagic Escherichia coli in the space environment, and especially mcr-1 positive enterohemorrhagic Escherichia coli, and can be applied to the preparation of medicines for controlling the enterohemorrhagic Escherichia coli infection in order to realize a broad-spectrum sterilization effect.

The invention discloses a bacteriophage. DNA (deoxyribonucleic acid) sequences of the bacteriophage are sequences shown as SEQ ID No.1. The bacteriophage has the advantages that the bacteriophage is broad in lysis spectrum, and different types of vibrio parahaemolyticus can be lysed by the bacteriophage; the bacteriophage is high in heat stability and can tolerate high temperatures in environments; the numbers of bacteria in samples can be obvious reduced in the short time after the fish samples contaminated by the vibrio parahaemolyticus are treated by the bacteriophage, accordingly, the factthat the vibrio parahaemolyticus in the samples can be killed by the bacteriophage is illustrated, effects of food safety protective agents can be realized by the bacteriophage, and the bacteriophagehas a broad application prospect.

The invention provides a method for establishing a Klebsiella pneumoniae phage inhibiting mouse mastitis model, comprising the following steps: A, preparation of Klebsiella pneumoniae bacterial liquid, B, preparation of Klebsiella pneumoniae phage culture liquid, C, Experimental animal treatment, D, collection and testing of experimental samples. Through this method, the present invention can more accurately find Klebsiella pneumoniae phages with wide lysis spectrum for mouse mastitis, strong bacteriostasis ability and no toxic or side effects, so as to provide experimental and theoretical basis for treating bovine mastitis.

The invention discloses a highly lytic Salmonella phage RDP‑SA‑19009, the preservation number is CGMCCNo.21993, and the gene length is 87591bp. After sequencing the whole gene, it is found that it does not have virulence genes and lysogenic genes; Under the high temperature condition of ℃ for 30min, the titer remains at 1.0×10 6 More than pfu / mL, it has higher tolerance to temperature; the Salmonella phage RDP-SA-19009 provided by the present invention can crack including Salmonella typhimurium, Salmonella Stanley, Salmonella enteritidis, Salmonella Kentucky, Salmonella typhi, Salmonella Shenfudenburg Pathogenic bacteria, including Salmonella, have the characteristics of wide lysis spectrum and high lysis rate. The phage can be used alone or in combination with other Salmonella phages and probiotics, which can effectively treat and prevent poultry salmonellosis .

The invention discloses a high-temperature-resistant potent proteus phage RDP‑SA‑20018, the preservation number is CGMCC No.21405, the full length of the gene is 42626bp, and it is found after sequencing the whole gene that it does not have virulence genes and lysogenic genes, The Proteus phage RDP‑SA‑20018 provided by the present invention has higher temperature tolerance and wider acid-base tolerance range, which is conducive to industrial production and preparation; it is found by lysis rate experiment and RTD experiment that the lysis of the bacteriophage The rate is 98%, and it still has a good cracking effect on pathogenic bacteria. The phage has the characteristics of excellent performance and safety. The phage can be used in the preparation of medicines for preventing and or treating diseases caused by proteus.

The invention discloses a stable and efficient Salmonella virulent phage RDP-SA-21004, the preservation number is CGMCC No. 22483. After the whole gene is sequenced, it is found that it does not have virulence genes and lysogenic genes; Stored for 10 months under conditions, its activity remains at 10 8 Above pfu / mL, it has strong stability; it can efficiently infect host bacteria, and its optimal multiplicity of infection is 0.1; it has a broad spectrum of ability to kill Salmonella, and also has a certain ability to lyse Escherichia coli. The phage can be used alone or in combination with other Salmonella phages and probiotics, which can effectively treat, prevent and reduce poultry salmonellosis.

The present invention discloses a Salmonella phage RDP-SA-21003 capable of lysing Escherichia coli at the same time, and the preservation number is CGMCC No.22494. After sequencing the whole gene, it is found that it does not have virulence genes and lysogenic genes; the present invention obtains The lysing efficiency of the phage to the host bacterium BS20078 is above 98.7%. When used alone, the lysing ability to Salmonella is relatively strong and the lysing spectrum is wide; With cracking ability.

The invention discloses an application of calcium ions in promoting the formation of marine bdellovibrio bdelloplasts, and belongs to the technical field of bdellovibrio. The present invention promotes the formation of Bdellovrio sp. Bdellovrio sp. BDE‑1 high-density Bdellovrio sp. Bdellovrio sp. by utilizing calcium ions, the factor that promotes the formation of Bdellovibrio leech plastids; The formation of hiruplasma all has obvious promoting effect, and the cracking performance of hiruplasma has not declined, and the shelf life of high-density hiruplasma group has been obviously prolonged compared with control group; The Vibrio that causes human infection and food poisoning can also inhibit the Vibrio that can cause biological diseases in aquaculture. At the same time, it also has a good antibacterial effect on other food-borne pathogens. It has a wide cracking spectrum and its cracking Compared with the mixture of Bdellovibrio BDE-1, the effect remains unchanged; the method for promoting the cultivation of the bdeloid plastids of the present invention is simple and easy, and can be popularized and used.

The invention belongs to the technical field of microbial control, and in particular relates to a combination of Vibrio parahaemolyticus phage VP-HYP MCS-1 and Halobacteriovorax sp.MCS-1 and their use in preventing and treating pathogenic Vibrio parahaemolyticus infection of prawns. application. The phage of Vibrio parahaemolyticus is the phage of Vibrio parahaemolyticus VP-HYP MCS-1, which has been deposited in the General Microbiology Center of the China Microorganism Culture Collection and Administration Commission, and the preservation number is CGMCC No.19693. Vibrio parahaemolyticus is Vibrio parahaemolyticus Halobacteriovorax sp.MCS‑1, which has been deposited in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee, and the preservation number is CGMCC No.19694. The virulent bacteriophage VP-HYP MCS-1 isolated and obtained by the present invention has strong host specificity and has the functions of lysing and killing Vibrio parahaemolyticus; the isolated and obtained leech vibrio Halobacteriovorax sp.MCS-1 has a wide host spectrum , has the effect of lysing and killing a variety of Vibrio including Vibrio parahaemolyticus; the Vibrio parahaemolyticus bacteriophage and leech vibrio provided by the present invention have good performance in preventing and controlling pathogenic Vibrio parahaemolyticus infection of prawns application prospects.

The invention belongs to the field of biotechnology, and discloses a pseudomonas aeruginosa bacteriophage and an application thereof in prevention of hemorrhagic pneumonia of a mink. The pseudomonas aeruginosa bacteriophage is preserved in China General Microbiological Culture Collection Center on April 14, 2014, has the preservation number of CGMCC No. 9102, and is classified and named pseudomonas aeruginosa bacteriophage. As for the application of the pseudomonas aeruginosa bacteriophage in prevention of hemorrhagic pneumonia of the mink, assume that the living space of each mink is 1m<3>; an ultrasonic spraying mode is adopted; the titer of the pseudomonas aeruginosa bacteriophage is 10<9>PFU / ml, and the dose for each mink is 10ml; hemorrhagic pneumonia of the mink can be prevented by using the pseudomonas aeruginosa bacteriophage once per month in a disease outbreak period. By adopting the ultrasonic spraying application mode, the bacteriophage can directly arrive in the lung where bacteria is colonized, thus improving the efficiency of controlling bacterial infection of the lung of an animal by the bacteriophage, and improving the breeding economic benefits.

The invention discloses a strong lysis bacteriophage for splitting NDM-1 positive Klebsiella pneumoniae clinical isolates, and an application of the strong lysis bacteriophage used as a biological preparation in the prevention and treatment of NDM-1 Klebsiella pneumoniae induced infection and pollution. The preservation number of the bacteriophage is CCTCC M 2015760, and the bacteriophage has a regularly icosahedral head and a telescopic tail, and belongs to a Myoviridae double-stranded DNA bacteriophage. The bacteriophage has strong kilign activity and wide bacteriophage lysis spectrum, can be used for preparing medicines for preventing and treating NDM-1 Klebsiella pneumoniae induced bacterium infection, and also can be used for killing NDM-1 positive Klebsiella pneumoniae in culture environment and medical environment.

The invention discloses a bactericide for preventing and curing crop bacterial diseasess as well as a preparation method and application thereof. In the preparation method, oligosaccharide is added into bdellovibrio bacteriovorus solution to be evenly mixed to obtain the bactericide for preventing and curing crop bacterial diseasess. The invention applies bdellovibrio bacteriovorus solution for preventing and curing crop bacterial diseases for the first time, fills the vacancy of researching and developing bdellovibrio bacteriovorus at home and abroad and adds new species for biopesticide. Asbdellovibrio bacteriovorus has the characteristics of broad cracking pattern and quick growth and has the outstanding capability of eliminating various pathogenicbacteria and bacteria, after combinedwith oligosaccharide, the bdellovibrio bacteriovorus strengthens crop resistance, greatly improves pesticide effect and enlarges the preventing and curing spectrum of bacterial diseases. The bactericide prepared by the invention has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, pathogenic bacteria have small possibility of producing resistance, so that the invention overcomes 3R problems brought by abusing antibiotics and avoids the defects of serious chemical pesticide pollution and ecological balance damage at present.

The invention relates to the field of microorganisms, and specifically provides Ralstonia solanacearum phage GP1 (Ralstonia solanacearum phage GP1), preservation number CCTCC M 2016633; GP2 (Ralstia solanacearum phage GP2), preservation number CCTCC M 2016634; Ralstonia solanacearum phage GP1; Phage GP3 (Ralstonia solanacearum phage GP3), deposit number CCTCC M 2016635. The advantages of the phage of the present invention include: it is a strict and potent phage and can effectively kill R. solanaceae from different plant host sources, especially R. solanaceae that causes ginger blast; it is non-toxic to normal microbial flora Function; its DNA cannot encode virulence genes; it has strong stability between pH 5 and pH 9; there is no order of magnitude change in potency when stored at 4°C for 1 year; it can effectively inhibit bacteria and kill bacteria at low potency. The bacteriophage of the present invention can prevent and treat crop bacterial wilt caused by R. solanacearum, especially ginger blast, provide excellent strain resources for the development of new bacterial wilt-resistant biological pesticides, and have good application and development prospects.

The invention discloses a bdellovibrio bacteriovorus for preventing and curing rice bacterial diseases and application thereof. The invention can obtain bdellovibrio bacteriovorus BDFM05 by separation and ultraviolet mutation. The bdellovibrio bacteriovorus BDFM05 is combined with oligosaccharide to obtain bdellovibrio bacteriovorus for preventing and curing crop bacterial diseases. The inventionapplies bdellovibrio bacteriovorus BDFM05 to prevent and cure crop bacterial diseases for the first time. The bdellovibrio bacteriovorus prepared by the method has better rice bacterial diseases preventing and curing effect than common Saisentong, has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, produces no pathogenic bacteria resistance, has no toxic or side effects to people and animals, improves the quality and safety of agricultural products and conforms to the requirements of people on healthy and green food and sustainable-development for agriculturalproduction.