Bactericide for preventing and curing crop bacterial diseases as well as preparation method and application thereof
A bacterial and crop technology, applied in the field of inoculants for preventing and treating bacterial diseases of crops and their preparation, can solve problems such as no effective methods for bacterial diseases of crops of leech vibrio, and achieve excellent eradication ability, wide cracking spectrum, and natural enemies. safe effect
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Embodiment 1
[0039] Embodiment 1 prevents and treats rice bacterial blight
[0040] (1) Preparation of Bdellovibrio bacterial liquid
[0041]The fermentation method of the Bdellovibrio liquid adopts the fermentation method of the high-density Bdellovibrio swimming body disclosed in the application number "200710031166.X", and the title is "Fermentation and Culture Technology of High-density Bdellovibrio Swimming Body" National Invention Patent Application: respectively Inoculate Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Institute of Microbiology, No. GIM1.172) in 3 Erlenmeyer flasks equipped with 100mL LB (Luria-Bertani) liquid medium, 200rpm, Cultivate on a shaker at 28°C for 18 hours, centrifuge the culture solution at 4°C and 8000rpm for 10min, and discard the supernatant. Add the precipitates to 3 containers containing 100mL DNB liquid medium (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, NaCl 30g, di...
Embodiment 2
[0053] Embodiment 2 prevents and treats rice stripe disease
[0054] (1) The preparation of Bdellovibrio bacterial liquid is the same as step (1) of Example 1.
[0055] (2) Preparation of bacterial agents for preventing and treating bacterial diseases of crops
[0056] Bdellovibrio BDS01, Bdellovibrio BDS02 and Bdellovibrio BDSM08 were mixed according to the number of bacteria 1:1 in pairs (that is, BDS01 and BDS02, BDS01 and BDSM08, BDS02 and BDSM08), and added to each liter of the mixed bacteria liquid 15g agar oligosaccharides and 15g dextran oligosaccharides were shaken for 30s with a magnetic stirrer to obtain bacterial agents A, B and C for preventing and treating bacterial diseases of crops;
[0057] Mix Bdellovibrio BDS01, Bdellovibrio BDS02 and Bdellovibrio BDSM08 according to the number of bacteria 1:1:1, add 15g agarose oligosaccharide and 15g dextran oligosaccharide to each liter of mixed bacteria solution, shake with a magnetic stirrer 30s, get bacterial agent D...
Embodiment 3
[0061] Embodiment 3 prevents and treats bacterial angular leaf spot of cucumber
[0062] (1) The preparation of Bdellovibrio bacterial liquid is the same as step (1) of Example 1.
[0063] (2) Preparation of bacterial agents for preventing and treating bacterial diseases of crops
[0064] Mix the Bdellovibrio BDS01 and Bdellovibrio BDSM08 bacteria solutions according to the number of bacteria 1:1, dilute with normal saline, the concentration is 10 3 pfu / mL, 10 5 pfu / mL, 10 7 pfu / mL and 10 9 There are four concentration levels of pfu / mL, then add 10g chitosan oligosaccharide and 20g dextran oligosaccharide to each liter of mixed bacterial solution in different concentrations of Bdellovibrio solutions, and shake it with a magnetic stirrer for 30s to obtain the control of bacterial diseases of crops The bacterial agents A, B, C and D of Bdellovibrio BDS01, BDS02, and BDSM08 were mixed according to the number of bacteria 1:1:1, diluted with normal saline, and the concentration...
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