135 results about "Salmonella typhi" patented technology

The invention discloses lactobacillus plantarum GLM101 and an application thereof in preparing a feed additive. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number CGMCC No. 11156. The lactobacillus plantarum GLM101 has very strong inhibiting ability for escherichia coli, staphylococcus aureus, salmonella typhi, vibrio vulnificus and aeromonas hydrophila, and also has excellent acid-resisting and cholate-resisting abilities. The lactobacillus plantarum GLM101 can be used for adjusting the microecological balance inside the intestines of animals, has the action of enhancing the nonspecific immunity function to prevent diseases, and also can provide trophic factors, promote digestive absorption of nutrients, promote growth of animals, and improve the feed conversion ratio.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The present invention belongs to the field of microbe detecting technology. The oligonucleotide probe for detecting common intestinal tract pathogenic bacteria is designed on 16S rRNA and 23S rRNA of bacteria, ipaH of dysentery bacillus giant plasmid, VipR of Salmonella typhi and other gene sequence, has length of 25-50 bp, and relatively high sensitivity and specificity. The oligonucleotide probe is suitable for detection based on nucleic acid hybridization principle, especially detection based on gene chip principle. Under certain use condition, it can detect Listeria, parahemolutic vibrio, Campylobacter, etc. It may be used in many aspects, such as disease diagnosis, environment detection, food poisoning detection, etc.

The present invention relates to methods for preventing or treating infectious diseases caused by extracellular microorganisms, such as bacteria and fungi, by systemically administering to a patient a compound containing gallium. The extracellular microorganisms targeted by the present methods include methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecalis (VRE), E. coli O157:H7, fluoroquinolone-resistant Salmonella typhi, and the like. Furthermore, in the present methods, gallium compounds can be co-administered with one or more conventional antimicrobial agents to treat infectious diseases with reduced risks of creating multi-drug resistant pathogens. The methods of the present invention is also applicable to those microorganisms, such as ulcer-causing Helicobacter pylori, complete eradication of which so far has been difficult to achieve.

The invention provides a broad-spectrum nucleic acid aptamer capable of identifying lipopolysaccharides of different Gram-negative bacterium sources. The broad-spectrum nucleic acid aptamer is obtained by directed screening on the basis of the capture-SELEX technology of label-free target molecules and immobilized nucleic acid libraries. A directed screening method of the broad-spectrum nucleic acid aptamer includes: using mouse salmonella typhi lipopolysaccharides as the unique target, using a biotin-avidin effect to fix random oligonucleotide libraries to Fe3O4 magnetic nano particles wrapped by avidin through biotinylated short complementary chains, performing incubation, separation, amplification, digestion single chain preparation, cloning sequencing and the like, adding complete active bacteria of salmonella and escherichia coli to serve as directed molecules to perform directed screening when the libraries are enriched to a certain degree, and one broad-spectrum nucleic acid aptamer capable of identifying four kinds of lipopolysaccharides is obtained through 15 rounds of repeated screening. The nucleic acid aptamer is applicable to the analysis and detection, separation and enriching, toxicity neutralizing and the like of the lipopolysaccharides in aspects of drinking water, food or clinical medicine and the like.

The invention relates to laundry detergent, in particular to efficient laundry detergent with bacterial inhibition and sterilization functions. The laundry detergent comprises components in percentage by weight as follows: 17%-23% of a surfactant, 1%-3% of an acid aid, 0.2%-0.5% of caustic soda flakes, 0.3%-0.5% of a chelating agent, 3%-6% of heartleaf houttuynia herb extract, 0.2%-0.4% of a preservative, 1%-2% of a thickening agent, 0.5%-1% of acorus gramineus essential oil, 1%-2% of fructus amomi essential oil, 1%-2% of licorice extract, 0.6%-1.5% of tea saponin, 0.4%-0.8% of lavender essential oil, 0.6%-1.2% of mint essential oil and the balance of deionized water. The efficient laundry detergent has an inhibiting function on streptococcus hemolyticus, staphylococcus aureus, hemophilus influenza, catarrhalis, pneumococcus, escherichia coli, shigella dysenteriae and salmonella typhi, also has an inhibiting function on various viruses and further has a body immunity enhancing function and an anti-tumor effect.

This invention relates to conjugates of the Vi polysaccharide of S. typhi with the carrier Pseudomonas aeruginosa recombinant exoprotein A (rEPA), and compositions thereof, and to methods of using of these conjugates and / or compositions thereof for eliciting an immunogenic response in humans, including responses which provide protection against, or reduce the severity of, S. typhi bacterial infections. The conjugates, and compositions thereof, are useful as vaccines to induce serum antibodies againt S. typhi and are useful to prevent and / or treat illnesses caused by S. typhi.

The invention provides an anti-chicken infectious bursal disease (IBD) recombinant protein subunit vaccine. The vaccine is a fusion protein having high immunogenicity of Salmonella typhimurium flagellin and an infectious bursal disease virus (VP2). The above flagellin + VP2 fusion protein is obtained through the expression of a recombinant baculovirus containing a flagellin + VP2 gene by utilizing a Bac-to-Bac baculovirus expression system. The recombinant baculovirus obtained through the system has a short period, and the expressed flagellin + VP2 fusion protein has high immune protection force.

The invention provides a flaggelline-fiber2 fusion protein as well as a preparation method and application thereof. The fusion protein is a fusion protein of a fowl adenovirus type 4 fiber2 protein with relatively high immunoprotection and a salmonella typhimurium flagellin. The preparation method comprises the following steps: cloning an artificially coded flaggin-fiber2 gene into a pFastBac-HA expression vector through fusion; carrying out gene transposition to form recombinant Bacmid, transfecting the recombinant Bacmid into Sf9 insect cells, expressing the fusion protein by utilizing a baculovirus system, and conducting identifying by virtue of indirect immunofluorescence IFA and Western blot. The period of obtaining the recombinant baculovirus through the system is short, and when SPFchicken are immunized by the flaggin-fibe2 fusion protein, results show that the flaggin-fibe2 fusion protein expressed by the baculovirus system has high immunoprotection capability.

The invention belongs to the field of antimicrobial peptides, and discloses an antimicrobial peptide based on a cell penetrating peptide Tat (49-57) and a synthetic method of the antimicrobial peptide. The antimicrobial peptide is Tat (YG), Tat (YY), Tat (FG) or Tat (FF), and a sequence of a peptide chain is successively as shown by SEQ ID No. 2 to No. 5. The antimicrobial peptide is synthesized by adopting Wang resin as a carrier, adopting Fmoc as an amino acid side chain protection base, adopting a DMF solution of piperidine as a deprotection reagent and adopting HBTU, HOBT and DIEA as amino acid condensing agents in a solid-phase synthetic method. The four derivative antimicrobial peptides have good inhibition efficiency for escherichia coli, salmonella typhimurium, Bacillus subtilis and staphylococcus aureus, and the inhibition rate for some bacteria can be higher than that of the cell penetrating peptide Tat (49-57); and moreover, the hemolytic activity of the four derivative microbial peptides is small, and the concentration when the bacteriostatic activity is played cannot reach the lowest hemolytic concentration.

An antigen-presenting system (APS) comprising one or more enterobacterial antigens in combination with a papaya mosaic virus (PapMV) or a virus like particle (VLP) derived from papaya mosaic virus isprovided. The PapMV or VLP included in the APS is capable of potentiating an immune response against said one or more enterobacterial antigens. The APS can be used, for example, as a vaccine against enterobacterial disease, such as typhoid fever. The one or more antigens comprised by the APS can be conjugated to a coat protein of the PapMV or PapMV VLP, or they may be non-conjugated (i.e. separatefrom the PapMV or PapMV VLP), or the APS can comprise both conjugated and non-conjugated antigens. Conjugation can be, for example, by genetic fusion with the coat protein, or binding via covalent, non-covalent or affinity means.

The present invention relates to an adjuvants, particularly to the use of a well-characterized plant based iridoid glycoside adjuvant from plant Picrorhiza kurroa, acting as an adjuvant against T-dependent antigen and specifically against HBsAg and typhoid antigens. The present invention also relates to the method of producing the iridoid glycoside adjuvant and the products utilizing such adjuvants for induction of cellular immunity. The adjuvants may be used alone or with specific antigens. The two antigens used in the study represents HBsAg, a recombinant antigen expressed in Pichia pastoris, and typhoid Vi polysaccharide purified from Salmonella typhi broth. These antigens are studied for their immunogenicity with the adjuvant iridoid glycoside adjuvant

The invention discloses a synthesis method of urea peroxide and applications of the urea peroxide in an antibacterial agent. The synthesis method comprises the following steps: adding hydrogen peroxide and urea into a reactor in proportion, and stirring at room temperature to dissolve hydrogen peroxide and urea; adding an additive into the reactor, and stirring at room temperature; and cooling andcrystallizing an solution in the reactor, filtering, and freeze-drying an obtained filter cake. The urea peroxide obtained by the invention is solid powder, is not easy to absorb moisture, is convenient to store and transport, is not easy to deteriorate, has a good sterilization effect, has good inhibition and killing effects on staphylococcus aureus, escherichia coli, bacillus sakazakii, salmonella typhimurium, listeria monocytogenes and vibrio parahaemolyticus, and is a potential broad-spectrum antibacterial agent.

The invention discloses a method for rapidly, quantitatively and simultaneously detecting a plurality of intestinal tract pathogenetic bacteria in an environmental water sample, which applies an intestinal tract pathogenetic bacteria PCR universal primer to build a real-time fluorescence quantitative polymerase chain reaction system for quantitatively measuring bacterial cell densities of Escherichia coli, comma bacilli, dysentery bacilli and salmonella typhi in the environmental water, and the method can shorten the time of water sample analysis from sampling to report completing to a plurality of hours, the measuring results are stable, and the repeatability is good. The standard deviation of the measuring results of the invention are all lower than the MF test results, the test results are stable, and the detecting repeatability is greatly improved.

The invention discloses a Chinese-Western combination sterilizing and disinfecting solution and a preparation method thereof, which belong to the technical field of medicine; the ingredients of the sterilizing and disinfecting solution of the invention are composed of the following raw materials: Puhuang, Campanula, Spirulina, Artemisia arvensis, Peach Blossom and Yahuazi , Jianweifeng, Lotus Pod, Saijingang, Marigold, Eucalyptus blue, Cinnabar Root, Catalpa Bark, Duckweed, Norfloxacin, Cresyl Sulfonic Acid and Triclosan; The treatment method is better than the simple use of traditional Chinese medicine and western medicine. It has the effects of killing insects and disinfecting, clearing away heat and detoxifying, antibacterial and anti-inflammatory, and can maximize the efficacy of Chinese medicine ingredients and western medicine. , has a good inhibitory effect on Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Proteus vulgaris, etc., with remarkable effect and low cost.

The present invention is related with the Microbiology field and particularly with a composition and a method for early detection, identification, differentiation and count of microscopic organisms, concretely Gram-negative microorganisms. The composition described in the invention consist on a mixture of substances of protein origin with a total nitrogen content from 9 to 20% and in relationship between 2:1 to 24:1, concerning to the content of inhibitors of the Gram-positive organisms. It contains a mixture of organic and inorganic substances that facilitate the differentiation of the Gram-negative organisms, being this mixture in a relationship from 0.5:1 to 2:1 concerning to the mixture of substances of protein origin. The referred composition allows the detection and differentiated count of E. coli and other coliform organisms due to the blue-greenish color of the colonies of these microorganisms on the orange bottom of the medium; Salmonella not typhi for the red color of the centers of the colonies on rosy bottom of the medium; Salmonella typhi and Proteus for the transparency of the colonies; Citrobacter and Klebsiella for the violet color of the colonies on the pink to orange bottom of the medium and Pseudomonas aeruginosa for the orange color with darker center of the colony, taking greenish pigmentation after 24 hours and producing greenish fluorescence under low ultraviolet light.

The invention relates to mouse typhus salmonella UF110lux and application of mouse typhus salmonella UF110lux in living body imaging. The strain is conserved in the China General Microbiological Culture Collection Centre specified by the State Intellectual Property Office on March 13, 2012, and the conservation number is CGMCCNo.5893. The mouse typhus salmonella UF110 is a strain obtained by the fact that a spv gene on a virulence plasmid is knockout, and can be used for researching the function of mouse typhus salmonella virulence gene spv as a control. The UF110lux is a strain which is constructed on the basis of the mouse typhus salmonella UF110 and has the stable biological luminescence property. According to the invention, the shortage of a traditional fluorescent mark is overcome, and the invasion, proliferation and diffusion of the mouse typhus salmonella in a living body of mice can be monitored dynamically in real time by using a living body imaging instrument. The invention provides a convenient tool for further study on a pathogenic mechanism of mouse typhus salmonella.

The present invention provides a Chinese medicine preparation for killing virus and its production process. Said production process includes the following steps: decocting the Chinese medicinal materials of sweet wormwood, bupleurum root, epimedium, cranesbill, mistletoe and licorice by adding water, filtering to obtain filtrate 1; pressing ginger and garlic bulb, filtering to obtain filtrate 2; uniformly mixing filtrate 1 and filtrate 2 according to weight portion to obtain the invented liquid medicine for killing virus; also can extract the filtrate 1 and filtrate 2 by using alcohol, concentrating and drying so as to obtain solid powder preparation. Said liquid medicine can be directly used for killing several viruses and various fungi of staphylococcus aureus, pneumococcus, salmonella, salmonella typhi, dysentery bacillus, bacillus pyocyaneus, streptococcus and vibrio cholerae, etc.

The invention provides a streptococcus, which is a new species of streptococcus, is named as Streptococcus zengyii sp.nov.HTS25, and is registered and preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC 15344. The Streptococcus zengyii sp.nov.HTS25 is cultured in a variety of fermentation media, and can generate strong bacteriostatic active substances, also has obvious bacteriostatic effect on pseudomonas aeruginosa and salmonella typhi, also has broad development space in control of diseases caused by pseudomonas aeruginosa and salmonella typhi, and has very good development and application prospects in antibacterial agents.

The invention discloses a small rhzomorph MccY and a preparation method and application thereof. The amino acid sequence of the small rhzomorph MccY is GGRGHIAEYFSGPITQVSFYG. Compared with MccJ25 which only has bactericidal activity on a small part of serotype salmonella such as enteritis and the like, the small rhzomorph MccY has a bacteriostatic / bactericidal effect on salmonella pullorum, salmonella typhimurium, salmonella kafenkii, salmonella infantis, salmonella London and shigella sonnei, and can kill other salmonella serotypes which cannot be killed by MccJ25; the small rhzomorph MccY has a remarkable bactericidal effect on salmonella typhimurium and salmonella pullorum which are common in livestock and poultry production, also has a bacteriostatic / bactericidal effect on shigella sonnei, overcomes the defect of narrow spectrum of small rhzomorph, has breakthrough significance, and has the potential of being used as an antibiotic substitute.

The invention discloses a polygonatum sibiricum endophyte and an application thereof. The polygonatum sibiricum endophyte is named HJ-1 and preserved at CCTCC (China Center For Type Culture Collection) in Wuhan University, Wuhan, China on March 28th, 2018, and the preservation number is CCTCC No:M2018159. Research proves that a fermentation product of the endophyte has the function of resisting Pseudomonas aeruginosa, Salmonella typhi and Bacillus thuringiensis.

The invention relates to a making method of a plant enzyme bactericidal agent.According to the making method, an efficient plant enzyme and a mixed plant enzyme are mixed at the mass concentration ratio of 1:4-1:6, the mixture is fermented and purified, and the plant enzyme bactericidal agent is obtained, wherein the content of the plant enzymes in the making method of the plant enzyme bactericidal agent is 70%-85%.The extremely strong bioactivity, catalysis and proteolysis of the plant enzymes are fully utilized for rapidly degrading soil organic matter needed by escherichia coli, salmonella typhi, Klebsiella pneumoniae, staphylococcus aureus and the like for existence into carbon dioxide and water, so that the bacteria die of the lack of living environments and nutrition intake, and thus the purposes of killing and restraining reproduction and propagation of harmful bacteria are achieved.The plant enzyme bactericidal agent is applied to the field of sterilization, purification and treatment of indoor polluted air and articles.

The invention provides an alligator mississrppinsis Cathelicidin-AM antibacterial peptide, a polynucleotide for coding the antibacterial peptide and an application of the antibacterial peptide. The antibacterial peptide has an amino acid sequence as shown in SEQ ID NO: 2. The polynucleotide for coding the antibacterial peptide has a nucleotide sequence containing the coded amino acid sequence as shown in SEQ ID NO: 2 or a complementary sequence of the nucleotide sequence. The antibacterial peptide has a remarkable inhibiting effect on various bacteria and has the minimal inhibitory concentration (MIC) of 62.5mug / mL for Echerichia coli (CMCC44102), Escherichia coli (ATCC25922) and Salmonella typhi (CMCC50115). The antibacterial peptide can be used for preparing feed additives, preservatives, agricultural biocontrol agents, therapeutic drug compositions for controlling microorganism infection and the like.

The invention provides a novel chimeric flagellin adjuvant of a helicobacter pylori multi-epitope vaccine. An active ingredient of the novel chimeric flagellin adjuvant of the helicobacter pylori multi-epitope vaccine is a polypeptide chain which mainly consists of regions D0 and D1 of salmonella typhimurium flagellin Flic, and regions D2 and D3 of helicobacter pylori flagellin F1aA. A nucleotide sequence of the protein adjuvant is synthesized through a PCR (polymerase chain reaction) technology, expressed in escherichia coli by utilizing an expression vector, and the adjuvant protein is obtained after protein purification.

The invention relates to Salmonella typhimurium X3337 / pRST98lux and its application in living imaging. The above strain is preserved in China General Microbiological Culture Collection Center assigned by State Intellectual Property Office on Feb., 5, 2013, and has a preservation number of CGMCC No.7252. Lux operons compose a sexual expression luciferase and a substrate, so the bacterial luminescence can be maintained without applying a screened pressure or substrate; and the above joint mutant strain provides conveniences for constructing a cell model and an animal model, and deeply researching the pathogenic mechanisms of Salmonella typhi virulent plasmids, related medicine research and screening, the infectious disease control and the like.

The invention discloses Weissella confuse YM5S2 with antibacterial and antioxidant activity and an application thereof. The Weissella confusa YM5S2 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 07th, 2020 at the address of Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard No.1, Beichen West Road, Chaoyang District, Beijing, and the accession number is CGMCC No:19308. The Weissella confusa YM5S2 with antibacterial and antioxidant activity can inhibit growth and propagation of enteric pathogenic bacteria such as salmonella typhimuriumand the like in vitro, has no hemolytic property, is sensitive to most antibiotics, has high safety and has high survival rate in gastric acid and intestinal high-bile salt environments.

The present invention relates to the field of combined vaccine compositions which are effective against all forms of meningococcal diseases as well as typhoid fever. The vaccine formulations comprising antigens from capsular polysaccharides of Neisseria meningitidis A, Neisseria meningitidis C, Neisseria meningitidis Y, Neisseria meningitidis W135, Neisseria meningitidis X, Salmonella typhi Vi capsular polysaccharide (ViPs) or capsular ViPs conjugated to a carrier protein tetanus toxoid (ViPs-TT). This invention is also related to improved methods, especially the use of an improved feed media and an improved method of downstream processing and industrial purification of capsular polysaccharides. The vaccine is free of any animal component or alcohol and is in absolute compliance with respect to the religious sentiments of various ethnic groups. The composition is highly effective and stable, yet cost-effective and affordable, especially for lower-middle income and low-income countries.

The invention provides an application of a dibenzimidazolamine compound to preparation of an antimicrobial medicine. The dibenzimidazolamine compound has a structure shown as formula (I), and is simple in structure. The dibenzimidazolamine compound is relatively good in in-vitro antimicrobial activity, and performs quite high inhibitory activity of gram positive bacteria, such as staphylococcus aureus, methicillin-resistant staphylococcus aureus, bacillus subtilis, and micrococcus luteus, gram negative bacteria, such as escherichia coli, proteus vulgaris, pseudomonas aeruginosa, and salmonella typhi, and fungi, such as candida utilis, aspergillus flavus, saccharomyces cerevisiae, candida albicans, and candida. The dibenzimidazolamine compound is used for preparing an antibacterial and / or antifungal medicine.