424 results about "Enteritides" patented technology

The invention discloses a multiple PCR identification kit of salmonella and five serotypes of salmonella. The invention provides a primer group for detecting salmonella and five serotypes of salmonella. The primer group comprises a primer pair 1, a primer pair 2, a primer pair 3, a primer pair 4, a primer pair 5 and a primer pair 6. Aiming at specific fragments of salmonella and its five important serotypes (such as salmonella typhimurium, enteritidis, agona, choleraesuis and pullorum), the six pairs of the primers for specific amplification are designed, the amplified fragments comprise 425bp of salmonella, 198bp of salmonella typhimurium, 302bp of salmonella enteritidis, 145bp of salmonella agona, 368bp of salmonella choleraesuis and 249bp of salmonella pullorum, and a sample gene group can be accordingly determined by multiple PCR and electrophoresis detection. Through six pairs of the specific primers and one-step multiple PCR, the salmonella and its five important serotypes can be identified. The multiple PCR identification kit has the characteristics of fast identification rate, low cost, easy operation and easy result determination.

The invention relates to the technical field of application of bacteroides fragilis, and in particular relates to application of bacteroides fragilis in preparing a composition for preventing and treating acute radiation enteritis. Experiments show that bacteroides fragilis is safe, nontoxic and strong in pharmacologic action and has a good preventing and treating effect to acute radiation enteritis, thereby indicating that the bacteroides fragilis has good edible and officinal prospects. The invention explores novel use of bacteroides fragilis and develops a novel application field. Bacteroides fragilis serving as probiotic can be used for preparing foods or medical compositions for preventing and treating acute radiation enteritis.

The invention relates to a traditional Chinese medicinal composition for treating gastrointestinal tract, in particular to a medicinal composition for treating gastrointestinal tract diseases such as acute and chronic gastritis, enteritis, gastric ulcer, superficial gastritis, gastric acid, gastrectasia, indigestion and the like. The invention also relates to a preparation of the medicinal composition. The traditional Chinese medicinal composition for treating gastrointestinal tract comprises the medicaments in percentage by weight: 10-25 parts of red ginseng, 10-23 parts of poria, 8-20 parts of bighead atractylodes rhizome, 10-25 parts of rhizoma ligustici wallichii, 10-25 parts of rhizoma atractylodis, 10-25 parts of cyperus rotundus, 10-25 parts of medicated leaven, 10-25 parts of gardenia, 10-23 parts of betelnut, 8-20 parts of radix aucklandiae, 5-15 parts of liquorice, 5-8 parts of malt and 10-25 parts of hawthorn. The medicinal composition for treating the gastrointestinal tract is orally taken and has the efficacies of regulating the flow of qi, comforting the stomach, diminishing inflammation, killing pain, repairing gastrointestinal mucosa and ulceration, enhancing gastrointestinal motility, adjusting gastric acid secretion, promoting digestive absorption and the like.

The invention relates to nutrition intestinal flora prevention and / or allergic disease treatment traditional Chinese mineral medicine and inorganic salt compound preparation, which is characterized inthat per 100g of the traditional Chinese mineral medicine and inorganic salt compound preparation is prepared from the following ingredients in proportion by weight (gram): 1 to 98g of traditional Chinese mineral medicine composition, 0 to 98g of oxides, 0 to 10g of sulfate, 0 to 10g of halogenide, 0 to 10g of carbonate, 0 to 10g of phosphate, 0 to 10g of silicate, 0 to 10g of borate, 0 to 10g ofsulfide, 0 to 10g of nitrate and 0 to 10g of molybdate. The body immunity can be improved through improving the diversity of the enteric microorganism and promoting the growth of various probiotics;the body immunity is improved; the allergic disease, inflammatory enteritidis and autism can be further cured.

The present invention relates to a novel bacteriophage, and more specifically to a bacteriophage that can specifically destroy one or more Salmonella spp. selected from a group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum. The present invention further relates to a composition containing the bacteriophage as an active ingredient for the prevention or treatment of infectious diseases such as salmonellosis and salmonella foodborne intoxication induced by Salmonella enteritidis or Salmonella typhimurium, fowl typhoid induced by Salmonella gallinarum, pullorum induced by Salmonella pullorum, etc. Moreover, the present invention relates to animal feeds, drinking water, detergent and disinfectant containing the bacteriophage as an inactive ingredient.

The invention discloses a salmonella enteritidis double knockout attenuated mutant and preparation as well as application thereof. The salmonella enteritidis double knockout attenuated mutant is obtained by knocking out crp gene and spiC gene of salmonella enteritidis C50041. The invention further discloses a preparation method as well as application of the salmonella enteritidis double knockout attenuated mutant. Further attenuation of the salmonella enteritidis attenuated mutant is realized. A foundation is laid for researching salmonella enteritidis attenuated live vaccines and live vector vaccines.

The invention discloses a Salmonella enteritidis nucleic acid standard sample as well as a building method and application thereof. The building method comprises the steps: strain culture, identification, preparation of a nucleic acid standard sample, drying of the nucleic acid standard sample, uniformity and stability inspection, qualitative assay, rating of the nucleic acid standard sample and other operations to obtain the Salmonella enteritidis nucleic acid standard sample. According to the invention, the condition that the pathogen nucleic acid standard sample is deficient in domestic food is solved, has important practical significance in realizing preparation technology of the pathogen nucleic acid standard sample in food and stability guarantee technology, positively developing the research and development of pathogen molecular biological detection standard sample in domestic food, and filling up the blank of the measuring field, and has a broad market in developing molecular biological detection working of pathogen in the food and food pathogen nucleic acid standard sample, and has greater economic benefits and social benefits.

The invention discloses a multifunctional probiotic, and belongs to the technical field of the preparation of probiotic preparation. The probiotic contains the following components active substances: Lactobacillus casei, Bacillus licheniformis, Bacillus subtilis, Bacillus cereus, Saccharomyces cerevisiae and Pseudomonas stutzeri. The multifunctional probiotic composition provided by the invention can be prepared into a solution for preparation of beneficial bacteria forage, can be used for deodorization of animal dung, prevention and treatment of enteritis and diarrhea of animals, and has wide range of application.

The invention provides a salmonella broad-spectrum virulent bacteriophage. The preservation number of the bacteriophage is CCTCC NO: M 2020839. The invention also provides a preparation process and application of the salmonella broad-spectrum virulent bacteriophage, and the preparation process comprises fermentation and extraction; in the step of fermentation, a salmonella gallinarum attenuated vaccine strain is used as host bacteria for bacteriophage fermentation. The bacteriophage XPARCPS02 disclosed by the invention can be used for cracking salmonella of various serotypes and also has different degrees of cracking effects on drug-resistant bacteria; the salmonella typhimurium lysate lysate has a cracking capability on salmonella typhimurium, salmonella typhimurium drug-resistant bacteria, salmonella gallinarum, salmonella enteritidis, salmonella enteritidis drug-resistant bacteria, salmonella typhimurium, salmonella pullorum, salmonella paratyphi A, salmonella paratyphi B and salmonella dubicularis.

The invention relates to bacillus subtilis having effects of relieving enteritis and promoting development of intestines. The bacillus subtilis is named as RZ001, the category name is Bacillus subtilis., the preservation No. is CGMCC NO:17116, the preservation date is 7, January, 2019, the preservation address is No.3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, and the preservation unit is China General Microbiological Culture Collection Center(CGMCC). The bacterial strain has the functions of repairing and strengthening mucosal barrier, has the function of treating inflammatory and allergic dermatosis enteritis, and can also promote the development of the intestines. The bacterial strain is hopeful to be used for preparing drugs and fermented foods for preventing andtreating intestine inflammation and promoting development, and has broad application prospect.

The present invention provides the method for the production of the egg containing antipathogenic bacteria specific antibodies (IGY) preventing gastritis, diarrhoea, and food poisoning by immunizing young hens with antigen proteins of E. coli causing enteritis, Helicobacter pylori causing gastritis, and Salmonella enteritidis and Salmonella typhimurium causing food poisoning. Simultaneously this invention also relates to the composition containing the specific IGY antibodies described above and the foodstuff such as the yoghurt and ice cream containing the antipathogenic IGY. Additionally, the present invention provides the separation method of the IGY containing protein powders from egg yolk. Particularly, this separation method involves diluting egg yolk with water at 1:1 ratio and adding the appropriate amount of ammonium sulphatewhich enables water-soluble protein and phospholipid to separate.