Saccharomyces cerevisiae and culture thereof and application thereof to feed

A technology of Saccharomyces cerevisiae and cultures, applied in animal feed, animal feed, applications, etc., can solve the problems that yeast cannot make full use of solid fermentation raw materials, the number of yeasts is not up to the standard, and the vitality of yeast strains is low, so as to increase feed nutrition value, reduction of animal morbidity, and the effect of enriching enzymes

Inactive Publication Date: 2018-05-29
潘韵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the commonly used solid fermentation method of yeast culture has the following problems: the activity of yeast strains is low, the number of yeasts is not up to the standard, and the yeast cannot make full use of the solid fermentation raw materials, resulting in low fermentation effect and high economic cost. The effect was not significant in animal feeding

Method used

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  • Saccharomyces cerevisiae and culture thereof and application thereof to feed
  • Saccharomyces cerevisiae and culture thereof and application thereof to feed
  • Saccharomyces cerevisiae and culture thereof and application thereof to feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation, screening, identification and preservation of Saccharomyces cerevisiae 5-1

[0035] 1. Obtaining the target strain

[0036] Collect surface soil samples from an apple orchard in Yantai City, Shandong, weigh 10g of the sample, collect it in a 150mL sterilized Erlenmeyer flask, add 90mL of sterile normal saline, add 30 sterile glass beads, and take 1mL of soil after shaking for 3-5 hours Add the suspension to the enrichment medium (enrichment medium: glucose 50g / L, (NH4) 2 SO 4 2g / L, KH 2 PO 4 2.5g / L, MgSO 4 ·7H 2 O1g / L, FeSO 4 ·7H 2 O 0.1g / L, yeast extract 0.5g / L, add sodium sulfite to 30mg / L after the above materials are sterilized. ), cultured at 28°C for 2d to 3d to obtain a proliferation culture medium, diluted the proliferation culture medium gradiently and then absorbed 0.1mL into the yeast extract powder peptone glucose medium, cultured at a constant temperature of 28°C for 2d to 3d, after the growth of colonies , Select a single c...

Embodiment 2

[0046] Embodiment 2: the preparation method of Saccharomyces cerevisiae 5-1 liquid fermented liquid

[0047] The preparation method of Saccharomyces cerevisiae 5-1 liquid fermentation liquid is as follows:

[0048] (1) Streak Saccharomyces cerevisiae 5-1 on yeast extract powder peptone glucose agar medium by streaking on a plate, culture at 30° C. for 48 hours, and obtain a single clone of the strain;

[0049] (2) Pick a single clone and inoculate it in yeast extract powder peptone glucose liquid medium, culture at 30°C for 15h, and the number of yeast reaches 2.0×10 8 CFU / mL, to obtain the seed solution;

[0050] (3) Get the above-mentioned seed liquid, inoculate it in the Saccharomyces cerevisiae liquid fermentation medium, the inoculum size is 2% (v / v), 30 ℃, 150rpm cultivates 24h, and the number of yeast reaches 3.0×10 8 CFU / mL.

Embodiment 3

[0051] Embodiment 3: the preparation method of Saccharomyces cerevisiae 5-1 culture

[0052] The preparation method of S. cerevisiae 5-1 culture is as follows:

[0053] (1) Weigh 60% of corn, 5% of bran, 5% of corn gluten, 5% of soybean meal, and 25% of distiller's grains according to the following mass percentages, then pulverize them, measure their water content, calculate the weight of dry matter, and then mix well , to obtain a substrate, adding glucose into the substrate according to 5% of dry matter weight, and adding water to adjust the water content to 40%.

[0054] (2) The fermented liquid prepared in Example 2 was inoculated with 1 billion yeasts per gram of dry matter.

[0055] (3) Ferment the substrate inoculated with the strains at 30-40°C for 72 hours, and then dry the solid fermented product at 50°C through a tube bundle dryer and an active drying tower at two stages of low temperature to obtain a yeast culture.

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Abstract

The invention discloses saccharomyces cerevisiae 5-1 which is collected in the Guangdong Microbial Culture Collection Center, with the collection number of GDMCC No: 60243. The invention further discloses a fermentation solution, a culture and a culture dilution prepared from the saccharomyces cerevisiae 5-1. A metabolite of the saccharomyces cerevisiae 5-1 has a very strong inhibitory effect on pathogenic bacteria (escherichia coli, staphylococcus aureus and salmonella enteritidis); when the saccharomyces cerevisiae 5-1 is used as a feed additive and is added into feed for feeding animals, the disease incidence of the animals can be effectively reduced, the growth performance of the animals can be improved, and the intestinal microflora of the animals can be significantly improved, so that the saccharomyces cerevisiae 5-1 is of a great significance in development of the breeding industry.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to the application of a strain of Saccharomyces cerevisiae and its culture in feed. Background technique [0002] With the continuous improvement of the scale and specialization of animal husbandry, while promoting the rapid development of animal husbandry, livestock and poultry diseases have become more and more complicated, causing great losses to the breeding industry. At present, adding antibiotics and other drugs to feed is a common means of disease prevention and treatment. However, this not only easily causes the normal flora of the gastrointestinal tract of animals to be out of balance, making the animals resistant to drugs, but also the residues of drugs will bring serious harm to the health of animals and humans. Among them, yeast culture, as a feed additive, has attracted widespread attention due to its advantages of no toxic side effects, no residual pollution, no drug res...

Claims

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Application Information

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IPC IPC(8): C12N1/18A23K10/12A23K10/18A23K50/30C12R1/865
CPCA23K10/12A23K10/18A23K50/30C12N1/18C12N1/185C12R2001/865
Inventor 李莉玲
Owner 潘韵
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