36 results about "Salmonella Paratyphi" patented technology

The invention discloses a specific double antibody sandwich method for detecting salmonella in food based on a monoclonal antibody, and belongs to the field of immunoassay. The specific double antibody sandwich method comprises the following steps: immunizing a 7-week-old BALB / c mouse by using smooth salmonella typhimurium LPS-coupled bovine serum albumin according to a sodium periodate method, and carrying out immunization, fusion and screening to obtain 12 monoclonal antibodies for specifically identifying salmonella LPS; marking the 12 monoclonal antibodies with horseradish peroxidase HRP respectively, and pairing the 12 monoclonal antibodies with salmonella paratyphi bacteria; using 8G3 as a coated antibody and 8G3-HRP as a detection antibody to establish the salmonella detecting specific double antibody sandwich method, so that salmonella paratyphi with the LOD of 1000000 CFU / mL can be detected. The specific double antibody sandwich method has cross reactions with all salmonella bacteria, but does not have cross sections with E.coli, E.coli O157:H7, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes, so that an overall, reliable, fast and efficient analysis method is provided for detection of the salmonella in the food.

The invention provides a salmonella broad-spectrum virulent bacteriophage. The preservation number of the bacteriophage is CCTCC NO: M 2020839. The invention also provides a preparation process and application of the salmonella broad-spectrum virulent bacteriophage, and the preparation process comprises fermentation and extraction; in the step of fermentation, a salmonella gallinarum attenuated vaccine strain is used as host bacteria for bacteriophage fermentation. The bacteriophage XPARCPS02 disclosed by the invention can be used for cracking salmonella of various serotypes and also has different degrees of cracking effects on drug-resistant bacteria; the salmonella typhimurium lysate lysate has a cracking capability on salmonella typhimurium, salmonella typhimurium drug-resistant bacteria, salmonella gallinarum, salmonella enteritidis, salmonella enteritidis drug-resistant bacteria, salmonella typhimurium, salmonella pullorum, salmonella paratyphi A, salmonella paratyphi B and salmonella dubicularis.

The present invention is drawn to a live, attenuated S. Paratyphi A strain, a live, attenuated S. Paratyphi A strain comprising a stabilized plasmid expression system, an S. Paratyphi conjugate vaccine, and methods of using these strains and conjugate vaccine.

ADP-ribosylating toxins from Neisseria meningitidis, Streptomyces coelicolor, Mycoplasma pneumoniae, Salmonella typhimurium, Salmonella paratyphi, and Streptococcus pyogenes are disclosed, together with mutant toxins and uses therefor. There is only a low level of sequence identity between these toxins and toxins such as cholera toxin and E. coli heat labile toxin.

OmpD of non-typhoidal Salmonella (NTS), e.g. as derivable from Salmonella Typhimurium, and immunogenic fragments thereof are proposed for use in treating or preventing NTS infection and / or disease. OmpD of S. paratyphi and fragments thereof are also now of interest for vaccine use.

The present invention is drawn to a live, attenuated S. Paratyphi A strain, a live, attenuated S. Paratyphi A strain comprising a stabilized plasmid expression system, and methods of using these strains.

The invention provides compositions and methods for preventing, treating and diagnosing infection by Salmonella enterica serovar typhi (S. typhi) and / or S. paratyphi, i.e., typhoid fever.

The invention discloses a pill containing anise oil and cinnamon oil. The pill comprises the following active pharmaceutical ingredients in parts by weight: 7-10 parts of anise oil and 3-5 parts of cinnamon oil. The pill has relatively strong inhibiting effects for staphylococcus aureus, pneumococcus, corynebacterium diphtheriae, bacillus subtilis, vibrio cholerae, typhoid bacillus, salmonella paratyphi, shigella dysenteriae, escherichia coli and common pathogenic bacteria, and is suitable for treatment for common diseases including influenza, upper respiratory infection, diarrhea, dysentery and the like. The pill further can promote gastrointestinal motility and relieve abdominal pain; the pill has the effect of raising leukocyte and boosting immunity, and is easy to prepare, and convenient to use.

The invention relates to fermentation production and preparation of an active product, belongs to the field of bioengineering and in particular relates to a prodigiosin (PG) producing strain as well as a preparation method and an application thereof. The PG producing strain is Proteus Vulgaris and is preserved on June 24th, 2013 in CGMCC (China General Microbiological Culture Collection Center) and has the preservation number of CGMCCNo.7815 and the preservation unit address of No.3 No.1 Courtyard, West Beichen Road, Chaoyang District, Beijing, Institute of Microbiology of Chinese Academy of Sciences. The PG producing strain can produce PG through fermentation, and an improved inorganic salt culture medium is adopted and is cultured for 18-24 hours at a culture temperature of 20-28 DEG C and a rotating speed of 150-300rpm, and yield of PG can be 80-120mg / L. The produced prodigiosin has an inhibitory activity to staphylococcus aureus, salmonella paratyphi, tritirachium album and pseudomonas aeruginosa and has a good application prospect in bio-preparation of the PG.

The present invention relates to a new cocktail of bacteriophages with specific lytic activity against Salmonella enteritidis, Salmonella typhimurium and Salmonella paratyphi B., for reducing, eliminating and / or preventing them in farm animals and animals from the poultry sector, such as poultry, hens and breeding hens, in addition to eggs. It may be administered as an additive in the feed, in water or by spray. Moreover, the cocktail may be used as a disinfectant in work areas of farms and abattoirs, and in processed foods, without affecting the organoleptic properties of the product.

The invention discloses a processing method of Baiji powders. The method is characterized in that the method comprises the following steps: 1) raw materials are taken; 2) freeze-dry; 3) sterilizing; 4) pulverize; 5) packaging that powder finished product. The invention can reach the follow quality requirements: 1, Bletilla polysaccharide >= 20%; 2, heavy metal residue <= 20 mg / kg; 3, detecting salmonella paratyphi-free; 4, bile salt-resistant gram-negative bacteria (104 CFU / g.

The invention discloses primers and method for simultaneously detecting various pathogenic bacteria. The nucleotide sequences of the primers are shown in the specification, wherein an invA primer pair is used for detecting salmonella infection and salmonella paratyphi, a hly primer pair is used for detecting Listeria monocytogenes, a toxR primer pair is used for detecting vibrio parahaemolyticus, an ipaH primer pair is used for detecting shigella flexneri, and a vcc primer pair is used for detecting non-O1 Vibrio cholerae. The invention also discloses a multiple PCR (Polymerase Chain Reaction) amplification system built by using all the primers pairs, the system can be used for quickly and accurately detecting whether the corresponding pathogenic bacteria exist in a sample to be detected or not, and the whole detection process costs less than 2 hours, so that the system is simple and convenient to use; as the specificity of each primer pair is very high, a false positive result is avoided; and the primers can be made into a kit and the like, thus being very suitable for the pathogenic bacteria detection of agricultural and sideline products.

The invention discloses a process for extracting salmonella paratyphi A lipopolysaccharide by utilizing a cold phenol method. The extracting process comprises the following steps that (a) thallus treatment is performed; (b) first-time extraction is performed; (c) second-time extraction is performed; (d) LPS extraction is performed. In the mode, LPS purity can be improved. Compared with the prior art, the LPS purity is improved on the premise that new devices and reagents are not increased and the final product yield is not influenced, and the purity, safety and effectiveness of a final product are improved.

The invention discloses a Salmonella phage RDP‑NSA‑19050 with a wide lysis spectrum, the preservation number is CGMCCNo.21415, and the gene length is 87591bp. After sequencing the whole gene, it is found that it does not have virulence genes and lysogenic genes. The invention provides The Salmonella phage RDP‑NSA‑19050 can lyse a variety of species including Stanley, Salmonella gallinarum typhi, Salmonella typhimurium, Salmonella paratyphi A, Salmonella paratyphi B, Salmonella typhi, Salmonella enteritidis, and Salmonella Kentucky. Pathogenic bacteria have the characteristics of wide lysis spectrum and high lysis rate. The phage can be used alone or in combination with other Escherichia coli, Salmonella phages and probiotics, which can effectively reduce the mortality of chickens infected with Salmonella.

The present invention discloses a Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.